茄子种质资源对黄萎病的抗性鉴定
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摘要
为筛选抗黄萎病茄子种质资源、对比野生茄或近缘野生茄与栽培茄对黄萎病抗性水平是否存在差异,利用苗期人工接种技术对15份野生或近缘野生茄和285份栽培茄种质资源的抗黄萎病性进行鉴定。结果共筛选获得抗病材料11份,占种质资源比例为3.67%,表明茄子种质资源整体对黄萎病抗性水平较差。野生或近缘野生茄资源中有10份材料表现抗病,占野生或近缘野生茄资源比例为66.67%,表明野生或近缘野生茄对黄萎病整体抗性较好。栽培茄资源中有1份材料表现抗病,占栽培茄资源比例为0.35%,表明栽培茄对黄萎病整体抗性较差。野生或近缘野生茄和栽培茄中抗性种质资源占所有鉴定材料的比例分别为3.33%和0.33%,表明野生或近缘野生茄对黄萎病的抗性水平远高于栽培茄。
To screen germplasm resources resistant to Verticillium wilt,and compare the difference in resis?tance to Verticillium wilt between wild eggplant or its wild relatives and cultivated eggplant,the resistance to Verticillium wilt of 15 wild eggplant or its wild relatives and 285 cultivated eggplant germplasm resources were identified using the artificial inoculation technique at the seedling stage.As a result,a total of 11 resistant mate?rials were screened,which accounted for 3.67% of the germplasm resources,indicating that the germplasm re?sources of eggplant had poor overall resistance to Verticillium wilt.10 wild eggplants or their wild relatives showed resistance to the disease,accounting for 66.67% of the wild eggplants or its wild relatives,indicating that the wild eggplants or its wild relatives had better overall resistance to Verticillium wilt.1 cultivated eggplant material showed resistance to the disease,accounting for 0.35% of the cultivated eggplant resources,indicating that cultivated eggplant had a poor overall resistance to Verticillium wilt.The percentages of resistant germplasm resources in wild eggplants or their wild relatives and cultivated eggplants were 3.33% and 0.33%,respectively,indicating that the resistance level of wild eggplants or their wild relatives to Verticillium wilt was much higher than that of cultivated eggplants.
To screen germplasm resources resistant to Verticillium wilt,and compare the difference in resis?tance to Verticillium wilt between wild eggplant or its wild relatives and cultivated eggplant,the resistance to Verticillium wilt of 15 wild eggplant or its wild relatives and 285 cultivated eggplant germplasm resources were identified using the artificial inoculation technique at the seedling stage.As a result,a total of 11 resistant mate?rials were screened,which accounted for 3.67% of the germplasm resources,indicating that the germplasm re?sources of eggplant had poor overall resistance to Verticillium wilt.10 wild eggplants or their wild relatives showed resistance to the disease,accounting for 66.67% of the wild eggplants or its wild relatives,indicating that the wild eggplants or its wild relatives had better overall resistance to Verticillium wilt.1 cultivated eggplant material showed resistance to the disease,accounting for 0.35% of the cultivated eggplant resources,indicating that cultivated eggplant had a poor overall resistance to Verticillium wilt.The percentages of resistant germplasm resources in wild eggplants or their wild relatives and cultivated eggplants were 3.33% and 0.33%,respectively,indicating that the resistance level of wild eggplants or their wild relatives to Verticillium wilt was much higher than that of cultivated eggplants.
引文
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[9]胡建坤,黄蓉,方荣,等.茄子黄萎病苗期人工接种技术研究[J].江西农业大学学报,2017,39(6):1131-1138.Hu J K,Huang R,Fang R,et al.Study on artificial inoculation technique of eggplant Verticillium wilt at seedling stage[J].Acta Agriculturae Universitatis Jiangxiensis,2017,39(6):1131-1138.
[10]肖蕴华,林柏青.茄子种质资源黄萎病抗性鉴定[J].中国蔬菜,1995(1):32-33.Xiao Y H,Lin B Q.Identification of resistance to Verticillium wilt of eggplant germplasm resources[J].CHINA VEGETA?BLES,1995(1):32-33.
[11]林密,付余,卢淑雯,等.黑龙江省茄子品种资源黄萎病抗性鉴定[J].北方园艺,2000(2):42-43.Lin M,Fu Y,Lu S W,et al.Identification of Verticillium wilt resistance of eggplant variety in Heilongjiang Province[J].Northern Horticulture,2000(2),42-43.
[12]曹必好,雷建军,王勇,等.栽培茄与野生茄种间杂交研究[J].园艺学报,2009,36(2):209-214.Cao B H,Lei J J,Wang Y,et al.Inter-specific hybridization between solanum and solanum torvum[J].Acta Horticulturae Si?nica,2009,36(2):209-214.
[13]孙日飞.蔬菜高效育种技术研究进展[J].中国蔬菜,2005(9):1-3.Sun R F.Research progress on highly effective breeding techniques of vegetables[J].China Vegetables,2005(9):1-3.
[14]于淼.河北省茄子黄萎病菌的分离鉴定及抗性资源筛选[D].保定:河北农业大学,2013.Yu M.Identification of the pathogens of Verticillium wilt on eggplant and screening of resistant germplasm[D].Baoding:He?bei Agricultural University,2013.
[15]邢国明,亢秀萍,姬青云,等.茄果类蔬菜嫁接栽培研究进展[J].沈阳农业大学学报,2000,31(1):144-146.Xing G M,Kang X P,Ji Q Y,et al.Advances in the research of cultivation of grafting aubergine and fruit vegetable[J].Jour?nal of Shenyang Agricultural University,2000,31(1):144-146.
[16]陈胜萍,邢国明,亢秀萍,等.嫁接栽培对茄子产量、品质和黄萎病抗性的影响[J].山西农业大学学报(自然科学版),2009,29(4):335-339.Chen S P,Xing G M,Kang X P,et al.Effect of grafting cultivation on eggplant’s yield,quality and resistance to Verticilliumwilt[J].J Shanxi Agric Univ(Natural Science Edition),2009,29(4):335-339.
[2]杨华,蔡立旺,潘群斌,等.棉花黄萎病研究进展浅述[J].江西棉花,2007,28(6):3-6.Yang H,Cai L W,Pan Q B,et al.The research progress of cotton Verticillium wilt[J].Jiangxi Cotton,2007,28(6):3-6.
[3]郭岩,田娟,王进涛.茄子黄萎病流行学研究概述[J].北方园艺,1999(5):32-33.Guo Y,Tian J,Wang J T.Summary of epidemiological study on eggplant Verticillium wilt[J].Northern Horticulture,1999(5):32-33.
[4]李宁.茄子黄萎病生物学特性级生物防治的初步研究[D].雅安:四川农业大学,2006.Li N.Preliminary study on biological characteristics and biological control of eggplant Verticillium wilt[D].Ya’an:SichuanAgricultural University,2006.
[5]沈火明,吴国民,凌明华,等.茄子黄萎病的发生与综合防治技术[J].蔬菜,2002(3):28-29.Shen H M,Wu G M,Ling M H,et al.Occurrence and integrated control of eggplant Verticillium wilt[J].Vegetables,2002(3):28-29.
[6]Hashimoto K.Studies on Verticillium wilt of eggplant[J].Bulletin of the Saitama Horticultural,1989,2(3):110.
[7]曾华兰,叶鹏盛,何炼,等.茄子品种资源抗黄萎病性鉴定评价[J].西南农业学报,2008,21(3):655-658.Zeng H L,Ye P S,He L.Resistance evaluation of eggplant resources to Verticillium wilt[J].Southwest China Journal of Agri?cultural Sciences,2008,21(3):655-658.
[8]Tzima A K,Paplomatas E J,Rauyaree P,et al.VdSNF1,the sucrose nonfermenting protein kinsae gene of Verticilliumdahliae,is required for virulence and expression of genes involved in cellwall degradation[J].Mol Plant Microbe Interact,2011,24(1):129-142.
[9]胡建坤,黄蓉,方荣,等.茄子黄萎病苗期人工接种技术研究[J].江西农业大学学报,2017,39(6):1131-1138.Hu J K,Huang R,Fang R,et al.Study on artificial inoculation technique of eggplant Verticillium wilt at seedling stage[J].Acta Agriculturae Universitatis Jiangxiensis,2017,39(6):1131-1138.
[10]肖蕴华,林柏青.茄子种质资源黄萎病抗性鉴定[J].中国蔬菜,1995(1):32-33.Xiao Y H,Lin B Q.Identification of resistance to Verticillium wilt of eggplant germplasm resources[J].CHINA VEGETA?BLES,1995(1):32-33.
[11]林密,付余,卢淑雯,等.黑龙江省茄子品种资源黄萎病抗性鉴定[J].北方园艺,2000(2):42-43.Lin M,Fu Y,Lu S W,et al.Identification of Verticillium wilt resistance of eggplant variety in Heilongjiang Province[J].Northern Horticulture,2000(2),42-43.
[12]曹必好,雷建军,王勇,等.栽培茄与野生茄种间杂交研究[J].园艺学报,2009,36(2):209-214.Cao B H,Lei J J,Wang Y,et al.Inter-specific hybridization between solanum and solanum torvum[J].Acta Horticulturae Si?nica,2009,36(2):209-214.
[13]孙日飞.蔬菜高效育种技术研究进展[J].中国蔬菜,2005(9):1-3.Sun R F.Research progress on highly effective breeding techniques of vegetables[J].China Vegetables,2005(9):1-3.
[14]于淼.河北省茄子黄萎病菌的分离鉴定及抗性资源筛选[D].保定:河北农业大学,2013.Yu M.Identification of the pathogens of Verticillium wilt on eggplant and screening of resistant germplasm[D].Baoding:He?bei Agricultural University,2013.
[15]邢国明,亢秀萍,姬青云,等.茄果类蔬菜嫁接栽培研究进展[J].沈阳农业大学学报,2000,31(1):144-146.Xing G M,Kang X P,Ji Q Y,et al.Advances in the research of cultivation of grafting aubergine and fruit vegetable[J].Jour?nal of Shenyang Agricultural University,2000,31(1):144-146.
[16]陈胜萍,邢国明,亢秀萍,等.嫁接栽培对茄子产量、品质和黄萎病抗性的影响[J].山西农业大学学报(自然科学版),2009,29(4):335-339.Chen S P,Xing G M,Kang X P,et al.Effect of grafting cultivation on eggplant’s yield,quality and resistance to Verticilliumwilt[J].J Shanxi Agric Univ(Natural Science Edition),2009,29(4):335-339.