25～45 Gy γ射线辐照对红细胞制品质量的影响研究
|
摘要
目的研究25～45 Gy γ射线辐照对红细胞制品质量的影响。方法选取20袋健康志愿者新鲜全血(每袋400mL),每袋分为4份,将其设为对照组(无γ射线辐照)、观察1组(25 Gy γ射线辐照)、观察2组(35 Gy γ射线辐照)和观察3组(45 Gy γ射线辐照),对比分析4组红细胞制品第0、7、14、21、28天的红细胞活性等。结果第0、7、14、21、28天4组的腺苷三磷酸(ATP)、2,3-二磷酸甘油酸(2,3-DPG)、游离血红蛋白(FHb)、红细胞最小抵抗力和最大抵抗力比较,差异均无统计学意义(P>0.05);观察1、2、3组的淋巴细胞增殖抑制率均较高,但组间比较,差异均无统计学意义(P>0.05);第7、14、21、28天观察1、2、3组的钾离子(K~+)水平比较,差异均无统计学意义(P>0.05),但均明显高于对照组,差异均有统计学意义(P<0.05)。结论应用25～45 Gy γ射线辐照红细胞制品对红细胞活性、功能均无明显影响,但能显著抑制淋巴细胞增殖,防止输血相关性移植物抗宿主病(TA-GVHD)发生。
Objective To studied the effect of 25-45 Gy gamma irradiation on the quality of red blood cell products.Methods A total of 20 bags of healthy volunteers were selected as fresh whole blood 400 mL each bag,and each bag was divided into 4 parts.The control group(no gamma ray irradiation),observation 1 groups(25 Gy gamma ray irradiation),observation 2 groups(35 Gy gamma ray irradiation)and observation 3 groups(45 Gy gamma ray irradiation)were observed,the erythrocyte activity of four groups of red blood cell products at 7th,14th,21th and 28th day were collected and compared.Results There was no significant difference in ATP,2,3-diphosphate glyceric acid(2,3-DPG),free hemoglobin(FHb),erythrocyte minimum resistance and maximum resistance between groups at 7th,14 th,21th and 28th day(P>0.05).The proliferation inhibition rate of the lymphocyte in the observation 1,2,3 groups were higher,however,there were no significant difference between groups(P>0.05).On the 7th,14th,21th and 28th day,there were no significant difference in potassium ion(K~+)levels among the three observation groups(P>0.05),but they were significantly higher than those in the control group,and the differences were statistically significant(P<0.05).Conclusion Radiation of erythrocyte products by 25-45 Gy gamma rays has no significant effect on erythrocyte activity and function,but it can inhibit the proliferation of lymphocytes and prevent the occurrence of TA-GVHD.
Objective To studied the effect of 25-45 Gy gamma irradiation on the quality of red blood cell products.Methods A total of 20 bags of healthy volunteers were selected as fresh whole blood 400 mL each bag,and each bag was divided into 4 parts.The control group(no gamma ray irradiation),observation 1 groups(25 Gy gamma ray irradiation),observation 2 groups(35 Gy gamma ray irradiation)and observation 3 groups(45 Gy gamma ray irradiation)were observed,the erythrocyte activity of four groups of red blood cell products at 7th,14th,21th and 28th day were collected and compared.Results There was no significant difference in ATP,2,3-diphosphate glyceric acid(2,3-DPG),free hemoglobin(FHb),erythrocyte minimum resistance and maximum resistance between groups at 7th,14 th,21th and 28th day(P>0.05).The proliferation inhibition rate of the lymphocyte in the observation 1,2,3 groups were higher,however,there were no significant difference between groups(P>0.05).On the 7th,14th,21th and 28th day,there were no significant difference in potassium ion(K~+)levels among the three observation groups(P>0.05),but they were significantly higher than those in the control group,and the differences were statistically significant(P<0.05).Conclusion Radiation of erythrocyte products by 25-45 Gy gamma rays has no significant effect on erythrocyte activity and function,but it can inhibit the proliferation of lymphocytes and prevent the occurrence of TA-GVHD.
引文
[1]肖欢,韩燕,翟建青,等.(60)Co-γ射线和电子束辐照对冷鲜鸡保鲜效果的异同性研究[J].核农学报,2018,32(7):1358-1367.
[2]赵彦涛,刘思扬,尹惠琼,等.γ射线终末辐照对同种异体肌腱病毒灭活效果及生物力学性能影响的研究[J].中国骨与关节杂志,2018,7(5):389-393.
[3]胡连训.高海拔地区悬浮红细胞经γ射线辐照后的储存探讨[J].中国输血杂志,2015,28(11):1370-1371.
[4]肖乐宇.60Coγ射线辐照对去白细胞悬浮红细胞质量的影响[J].新乡医学院学报,2017,34(9):813-814.
[5]曹荣祎,苏适,于洪敏,等.γ射线对红细胞辐照时机及辐照后保存的研究分析[J].中国实验诊断学,2015,19(12):2082-2086.
[6]何梅,高杰,贾凯,等.60 Coγ射线辐照对大蒜农艺性状与叶片特征的影响[J].中国农学通报,2018,34(1):56-60.
[7]宋建,赵鹃,张利,等.国产某型137Csγ射线辐(下转第2125页)照仪辐照血液制品的应用研究[J].临床血液学杂志(输血与检验),2015,28(6):1037-1039.
[8]周炜鑫,郭天虹,黄远帅.γ射线和紫外线应用于血液制剂中病原体和白细胞的灭活[J].重庆医学,2016,45(28):4005-4007.
[9]金宇林,吴洁莹,陆琰,等.6.0Gy X射线辐照所致BALB/c小鼠造血系统损伤的动态检测[J].中国医药导报,2017,14(13):13-16.
[10]WINTER KM,JOHNSON L,WEBB RG,et al.Gamma-irradiation of deglycerolized red cells does not significantly affect in vitro quality[J].Vox Sanguinis,2015,109(3):231-238.
[11]KHOSHBIN AR,MOHAMADABADI F,VAFAEIAN F,et al.The effect of radiotherapy and chemotherapy on osmotic fragility of red blood cells and plasma levels of malondialdehyde in patients with breast cancer[J].Rep Pract Oncol Radiother,2015,20(4):305-308.
[12]MELI A,HANCOCK V,DOUGHTY H,et al.Investigation of the quality of stored red blood cells after simulated air drop in the maritime environment[J].Transfusion,2018,58(2):423-429.
[13]ACKER JP,ALMIZRAQ RJ,MILLAR D,et al.Screening of red blood cells for extracellular vesicle content as a product quality indicator[J].Transfusion,2018,58(9):2217-2226.
[14]SUN K,D′ALESSANDRO A,XIA Y.Purinergic control of red blood cell metabolism:novel strategies to improve red cell storage quality[J].Blood Transfus,2017,15(6):535-542.
[15]AMIRAL J,SEGHATCHIAN J.Blood derived products in pediatrics:new laboratory tools for optimizing potency assignment and reducing side effects[J].Transfus Apheresis Sci,2017,56(2):107-117.
[2]赵彦涛,刘思扬,尹惠琼,等.γ射线终末辐照对同种异体肌腱病毒灭活效果及生物力学性能影响的研究[J].中国骨与关节杂志,2018,7(5):389-393.
[3]胡连训.高海拔地区悬浮红细胞经γ射线辐照后的储存探讨[J].中国输血杂志,2015,28(11):1370-1371.
[4]肖乐宇.60Coγ射线辐照对去白细胞悬浮红细胞质量的影响[J].新乡医学院学报,2017,34(9):813-814.
[5]曹荣祎,苏适,于洪敏,等.γ射线对红细胞辐照时机及辐照后保存的研究分析[J].中国实验诊断学,2015,19(12):2082-2086.
[6]何梅,高杰,贾凯,等.60 Coγ射线辐照对大蒜农艺性状与叶片特征的影响[J].中国农学通报,2018,34(1):56-60.
[7]宋建,赵鹃,张利,等.国产某型137Csγ射线辐(下转第2125页)照仪辐照血液制品的应用研究[J].临床血液学杂志(输血与检验),2015,28(6):1037-1039.
[8]周炜鑫,郭天虹,黄远帅.γ射线和紫外线应用于血液制剂中病原体和白细胞的灭活[J].重庆医学,2016,45(28):4005-4007.
[9]金宇林,吴洁莹,陆琰,等.6.0Gy X射线辐照所致BALB/c小鼠造血系统损伤的动态检测[J].中国医药导报,2017,14(13):13-16.
[10]WINTER KM,JOHNSON L,WEBB RG,et al.Gamma-irradiation of deglycerolized red cells does not significantly affect in vitro quality[J].Vox Sanguinis,2015,109(3):231-238.
[11]KHOSHBIN AR,MOHAMADABADI F,VAFAEIAN F,et al.The effect of radiotherapy and chemotherapy on osmotic fragility of red blood cells and plasma levels of malondialdehyde in patients with breast cancer[J].Rep Pract Oncol Radiother,2015,20(4):305-308.
[12]MELI A,HANCOCK V,DOUGHTY H,et al.Investigation of the quality of stored red blood cells after simulated air drop in the maritime environment[J].Transfusion,2018,58(2):423-429.
[13]ACKER JP,ALMIZRAQ RJ,MILLAR D,et al.Screening of red blood cells for extracellular vesicle content as a product quality indicator[J].Transfusion,2018,58(9):2217-2226.
[14]SUN K,D′ALESSANDRO A,XIA Y.Purinergic control of red blood cell metabolism:novel strategies to improve red cell storage quality[J].Blood Transfus,2017,15(6):535-542.
[15]AMIRAL J,SEGHATCHIAN J.Blood derived products in pediatrics:new laboratory tools for optimizing potency assignment and reducing side effects[J].Transfus Apheresis Sci,2017,56(2):107-117.