40种苦苣苔科植物毛蕊花糖苷与总黄酮含量的测定
|
摘要
为给苦苣苔科植物的药用研究提供依据,采用反相高效液相色谱法和紫外分光光度法测定苦苣苔科4属40种植物的毛蕊花糖苷与总黄酮含量。结果表明:40种苦苣苔科植物中有30种含有毛蕊花糖苷,其中只有半蒴苣苔属11种植物全部含有,吊石苣苔属不含有毛蕊花糖苷,且半蒴苣苔属在4个属间分布含量较高,平均含量为0.078%;吊石苣苔属分布含量最低,平均含量为0.004 4%。40种苦苣苔科植物中均含有丰富的黄酮类化合物,其含量为0.31%~7.84%。报春苣苔属、半蒴苣苔属、吊石苣苔属和芒毛苣苔属的黄酮含量分布均无明确规律,含量高低差异较大。其中,半蒴苣苔属植物毛蕊花糖苷和总黄酮平均含量均最高,分别为0.078%和4.53%。
To provide references for studying on Gesneriaceae plants,the acteoside and total flavonoids contents in 40 kinds(four genera)of Gesneriaceae plants were measured by reversed phase high performance liquid chromatography and UV spectrophotometry determination separately. Results:Acteoside contained in 30 kinds of Gesneriaceae plants,all the 11 plant species of the genus Hemiboea contained acteoside while Aeschynanthus contained no acteoside.And the content in Hemiboea was higher among the four genera,0.078% on average,while that in Aeschynanthuswas the lowest,merely 0.0044% on average.All the 40 Gesneriaceae plants contained abundant flavonoids(0.31% ~7.84%).Flavonoids contents in Primulina,Hemiboea,Lysionotus and Aeschynanthus varied greatly and had no obvious regulation.Acteoside and total flavonoids contents were all the highest in Hemiboea plants,0.078% and 4.53%respectively.
To provide references for studying on Gesneriaceae plants,the acteoside and total flavonoids contents in 40 kinds(four genera)of Gesneriaceae plants were measured by reversed phase high performance liquid chromatography and UV spectrophotometry determination separately. Results:Acteoside contained in 30 kinds of Gesneriaceae plants,all the 11 plant species of the genus Hemiboea contained acteoside while Aeschynanthus contained no acteoside.And the content in Hemiboea was higher among the four genera,0.078% on average,while that in Aeschynanthuswas the lowest,merely 0.0044% on average.All the 40 Gesneriaceae plants contained abundant flavonoids(0.31% ~7.84%).Flavonoids contents in Primulina,Hemiboea,Lysionotus and Aeschynanthus varied greatly and had no obvious regulation.Acteoside and total flavonoids contents were all the highest in Hemiboea plants,0.078% and 4.53%respectively.
引文
[1]李振宇,王印政.中国苦苣苔科植物[M].郑州:河南科学技术出版,2004.
[2]李从瑞,安明态,姜运力,等.贵州省苦苣苔科植物资源现状与利用研究[J].种子,2014,33(10):58-60.
[3]吕惠珍,余丽莹,黄宝优,等.广西苦苣苔科药用植物资源[J].中国民族民间医药,2010(7):6-10.
[4]白贞芳,王晓琴,肖培根,等.广西苦苣苔科植物传统药物学调查[J].中药材,2012,35(1):20-23.
[5]徐文芬,何顺志.贵州唇形科、苦苣苔科药用植物资源的调查研究[J].贵州科学,2005(4):48-53.
[6]陈林.四种苦苣苔科植物的生物活性研究[D].郑州:河南大学,2010.
[7]白贞芳,王晓琴,肖培根,等.苯乙醇苷类化合物在苦苣苔科药用植物中分布规律[J].中国中药杂志,2013,38(24):4267-4270.
[8]高莉,彭晓明,霍仕霞,等.毛蕊花糖苷改善D-半乳糖致亚急性衰老小鼠脑损伤的作用[J].中草药,2013,44(24):81-85.
[9]陈薇娜,李飞,朱盼盼,等.毛蕊花糖苷对新生大鼠体外培养成骨细胞增殖与分化作用研究[J].海峡药学,2012,24(4):23-24.
[10]李婷婷,易超凡.不同来源菊花总黄酮含量及其抗氧化活性研究[J].辽宁中医药大学学报,2015,15(7):72-74.
[11]曹秦,吴辉,张蓓蓓,等.黄酮类化合物在防治神经退行性疾病中作用的研究进展[J].中国药理学与毒理学杂志,2015,29(3):457-463.
[12]程欢欢,刘晓平,陈红,等.生物类黄酮抗癌作用研究综述[J].医学理论与实践,2015,28(15):1997-1999.
[13]王绍云,唐文华,曹晖.不同采收期吊石苣苔中石吊兰素含量的动态研究[J].凯里学院学报,2007,25(6):46-47.
[14]陈娟,刘霞.高效液相色谱法测定毛泡桐花中麦角甾苷(毛蕊花糖苷)的含量[J].药物分析杂志,2004,24(2):123-125.
[15]李文斌.HPLC法测定麦味地黄丸中毛蕊花糖苷的含量[J].中国药房,2014,28(25):2665-2667.
[16]郑晓珂,李军,冯卫生.苦苣苔科植物研究进展[J].中国新药杂志,2003,12(4):261-263.
[17]刘辉鑫,袁柯,程存归.红花芒毛苣苔总黄酮提取工艺研究[J].中成药,2012,34(5):956-958.
[18]邓志军,刘若轩,李常青,等.毛蕊花糖苷对高脂模型大鼠血脂及血管内皮细胞Bax-Bcl2表达的影响[J].广东药学院学报,2015,31(5):625-628.
[19]陈微娜,李飞.毛蕊花糖苷对新生大鼠体外培养成骨细胞增殖与分化作用研究[J].海峡药学,2012,24(4):23-24.
[20]吕慧珍,潘春柳.苦苣苔科植物资源分布及其研究利用概况[C].2009中国植物园学术年会论文集,2009:100-103.
[2]李从瑞,安明态,姜运力,等.贵州省苦苣苔科植物资源现状与利用研究[J].种子,2014,33(10):58-60.
[3]吕惠珍,余丽莹,黄宝优,等.广西苦苣苔科药用植物资源[J].中国民族民间医药,2010(7):6-10.
[4]白贞芳,王晓琴,肖培根,等.广西苦苣苔科植物传统药物学调查[J].中药材,2012,35(1):20-23.
[5]徐文芬,何顺志.贵州唇形科、苦苣苔科药用植物资源的调查研究[J].贵州科学,2005(4):48-53.
[6]陈林.四种苦苣苔科植物的生物活性研究[D].郑州:河南大学,2010.
[7]白贞芳,王晓琴,肖培根,等.苯乙醇苷类化合物在苦苣苔科药用植物中分布规律[J].中国中药杂志,2013,38(24):4267-4270.
[8]高莉,彭晓明,霍仕霞,等.毛蕊花糖苷改善D-半乳糖致亚急性衰老小鼠脑损伤的作用[J].中草药,2013,44(24):81-85.
[9]陈薇娜,李飞,朱盼盼,等.毛蕊花糖苷对新生大鼠体外培养成骨细胞增殖与分化作用研究[J].海峡药学,2012,24(4):23-24.
[10]李婷婷,易超凡.不同来源菊花总黄酮含量及其抗氧化活性研究[J].辽宁中医药大学学报,2015,15(7):72-74.
[11]曹秦,吴辉,张蓓蓓,等.黄酮类化合物在防治神经退行性疾病中作用的研究进展[J].中国药理学与毒理学杂志,2015,29(3):457-463.
[12]程欢欢,刘晓平,陈红,等.生物类黄酮抗癌作用研究综述[J].医学理论与实践,2015,28(15):1997-1999.
[13]王绍云,唐文华,曹晖.不同采收期吊石苣苔中石吊兰素含量的动态研究[J].凯里学院学报,2007,25(6):46-47.
[14]陈娟,刘霞.高效液相色谱法测定毛泡桐花中麦角甾苷(毛蕊花糖苷)的含量[J].药物分析杂志,2004,24(2):123-125.
[15]李文斌.HPLC法测定麦味地黄丸中毛蕊花糖苷的含量[J].中国药房,2014,28(25):2665-2667.
[16]郑晓珂,李军,冯卫生.苦苣苔科植物研究进展[J].中国新药杂志,2003,12(4):261-263.
[17]刘辉鑫,袁柯,程存归.红花芒毛苣苔总黄酮提取工艺研究[J].中成药,2012,34(5):956-958.
[18]邓志军,刘若轩,李常青,等.毛蕊花糖苷对高脂模型大鼠血脂及血管内皮细胞Bax-Bcl2表达的影响[J].广东药学院学报,2015,31(5):625-628.
[19]陈微娜,李飞.毛蕊花糖苷对新生大鼠体外培养成骨细胞增殖与分化作用研究[J].海峡药学,2012,24(4):23-24.
[20]吕慧珍,潘春柳.苦苣苔科植物资源分布及其研究利用概况[C].2009中国植物园学术年会论文集,2009:100-103.