2种石山苣苔属植物叶插繁育研究
|
摘要
以河池石山苣苔Petrocodon hechiensis (Y.G.Wei,Yan Liu&F.Wen) Y.G.Wei&Mich.M?ller、靖西石山苣苔Pet. jingxiensis (Yan Liu,H.S.Gao&W.B.Xu) A.Weber&Mich.M?ller这2种石山苣苔属植物的叶片为试验材料,在不同基质上进行扦插繁育,并对3种基质组合的扦插效果进行对比分析,比较各扦插基质的优劣,筛选高效的扦插繁育方法。结果表明,在喷施0.2%多菌灵并对插穗经100 mg/L IAA溶液浸泡1 h后,采用水苔作为扦插基质时,2种石山苣苔属植物扦插生根情况较好,生根率也最高(河池石山苣苔为59%,靖西石山苣苔为62%),但出芽阶段会由于营养不良导致部分插穗出现死亡的现象;而采用水苔+蛭石+珍珠岩+泥炭土(体积比0.5∶1∶1∶1)为基质时,插穗成苗率较高(河池石山苣苔为50%,靖西石山苣苔为60%)。可见,河池石山苣苔与靖西石山苣苔的叶插繁殖可以将水苔作为生根基质,待插穗出芽后,以水苔+蛭石+珍珠岩+泥炭土(体积比0.5∶1∶1∶1)作为移栽基质,可获得较好的成苗效果。
The leaves of Petrocodon hechiensis(Y.G.Wei,Yan Liu & F.Wen) Y.G.Wei & Mich.M?ller and Pet.jingxiensis(Yan Liu,H.S.Gao & W.B.Xu) A.Weber & Mich.M?ller were used as experimental materials to carry out cutting propagation on different substrates,and the cutting effects of the three matrix combinations were compared and analyzed. The advantages and disadvantages of each cutting matrix were analyzed,and the efficient cutting propagation method was screened. The results showed that when 0.2% carbendazim was sprayed and the cuttings were immersed in 100 mg/L IAA solution for 1 h and the sphagnum moss was used as the cutting substrate,the rooting of the two species of the genus Petrcodon Hance was better,and the rooting rate was also the highest(59% in Petrocodon hechiensis,and 62% in Petrocodon jingxiensis),but some of the cuttings will die due to nutritional deficiency during the budding stage. When using sphagnum moss + vermiculite + perlite + peat soil(volume ratio 0.5∶1∶1∶1) as the substrate,the seedling rate of cuttings was higher(50% for Petrocodon hechiensis and 60% for Petrocodon jingxiensis). It can be seen that the sphagnum moss can be used as the rooting substrate,and after the sprouting of the cuttings,the mulberry + vermiculite + perlite + peat soil(volume ratio 0.5∶1∶1∶1)is used as the transplanting substrate,a better seedling effect can be obtained.
The leaves of Petrocodon hechiensis(Y.G.Wei,Yan Liu & F.Wen) Y.G.Wei & Mich.M?ller and Pet.jingxiensis(Yan Liu,H.S.Gao & W.B.Xu) A.Weber & Mich.M?ller were used as experimental materials to carry out cutting propagation on different substrates,and the cutting effects of the three matrix combinations were compared and analyzed. The advantages and disadvantages of each cutting matrix were analyzed,and the efficient cutting propagation method was screened. The results showed that when 0.2% carbendazim was sprayed and the cuttings were immersed in 100 mg/L IAA solution for 1 h and the sphagnum moss was used as the cutting substrate,the rooting of the two species of the genus Petrcodon Hance was better,and the rooting rate was also the highest(59% in Petrocodon hechiensis,and 62% in Petrocodon jingxiensis),but some of the cuttings will die due to nutritional deficiency during the budding stage. When using sphagnum moss + vermiculite + perlite + peat soil(volume ratio 0.5∶1∶1∶1) as the substrate,the seedling rate of cuttings was higher(50% for Petrocodon hechiensis and 60% for Petrocodon jingxiensis). It can be seen that the sphagnum moss can be used as the rooting substrate,and after the sprouting of the cuttings,the mulberry + vermiculite + perlite + peat soil(volume ratio 0.5∶1∶1∶1)is used as the transplanting substrate,a better seedling effect can be obtained.
引文
[1]M?LLER M,韦毅刚,温放,等.得与失:苦苣苔科新的属级界定与分类系统——中国该科植物之变[J].广西植物,2016,36(1):44-60.
[2]中国科学院中国植物志编辑委员会.中国植物志:第69卷[M].北京:科学出版社,1990:271-273.
[3]温放,李湛东.苦苣苔科(Gesneriaceae)植物研究进展[J].中国野生植物资源,2006,25(1):1-6.
[4]付传明,江海涛,黄宁珍,等.桂黔吊石苣苔的组织培养与快速繁殖[J].广西植物,2014,34(6):874-878.
[5]姚绍嫦,蓝祖栽,凌征柱.线柱苣苔无菌萌发及快速繁殖研究[J].中国农学通报,2014,30(31):280-284.
[6]黄宁珍,付传明,赵志国,等.桂林小花苣苔离体快速繁殖技术[J].植物学报,2010,45(6):744-750.
[7]文弢,娄丽,侯娜,等.大苞短毛唇柱苣苔离体培养和快速繁殖[J].天然产物研究与开发,2016,28:350-353.
[8]温放,李湛东,张启翔.牛耳朵的离体培养和快速繁殖[J].植物生理学通讯,2006,42(5):906.
[9]潘春柳,彭玉德,吕惠珍.广西特有药用植物弄岗唇柱苣苔叶插繁殖研究[J].北方园艺,2011(1):205-207.
[10]周太久,黄仕训,邓涛,等.6种唇柱苣苔属植物叶插繁殖试验[J].南方农业学报,2012,43(3):356-359.
[11]杨平,陆婷,邱志敬.濒危植物秦岭石蝴蝶的叶插繁殖研究[J].北方园艺,2016(11):57-60.
[12]邱志敬,邹纯清,谭小龙,等.苦苣苔科植物的扦插繁育研究[J].北方园艺,2015(11):60-65.
[13]邓涛,何永艳,周太久,等.外源IBA对5种苦苣苔科植物扦插繁殖的影响[J].经济林研究,2013,31(3):68-71.
[14]唐文秀,黄仕训,隗红燕,等.石灰岩植物桂林唇柱苣苔叶插繁殖研究[J].西南农业学报,2009,22(5):1395-1399.
[15]刘伟,曹晓慧.吊石苣苔扦插繁殖研究[J].北方园艺,2010(2):116-118.
[16]卢永彬,黄俞松,许为斌,等.石山苣苔属(苦苣苔科)花型态演化及分类学意义[J].广西植物,2017,37(10):1227-1239.
[17]吴昊天,徐双双,杨世勇,等.岩溶穴居性陆氏石山苣苔群落物种组成与生态为分析研究[J].广西植物,2017,10(37):1240-1249.
[2]中国科学院中国植物志编辑委员会.中国植物志:第69卷[M].北京:科学出版社,1990:271-273.
[3]温放,李湛东.苦苣苔科(Gesneriaceae)植物研究进展[J].中国野生植物资源,2006,25(1):1-6.
[4]付传明,江海涛,黄宁珍,等.桂黔吊石苣苔的组织培养与快速繁殖[J].广西植物,2014,34(6):874-878.
[5]姚绍嫦,蓝祖栽,凌征柱.线柱苣苔无菌萌发及快速繁殖研究[J].中国农学通报,2014,30(31):280-284.
[6]黄宁珍,付传明,赵志国,等.桂林小花苣苔离体快速繁殖技术[J].植物学报,2010,45(6):744-750.
[7]文弢,娄丽,侯娜,等.大苞短毛唇柱苣苔离体培养和快速繁殖[J].天然产物研究与开发,2016,28:350-353.
[8]温放,李湛东,张启翔.牛耳朵的离体培养和快速繁殖[J].植物生理学通讯,2006,42(5):906.
[9]潘春柳,彭玉德,吕惠珍.广西特有药用植物弄岗唇柱苣苔叶插繁殖研究[J].北方园艺,2011(1):205-207.
[10]周太久,黄仕训,邓涛,等.6种唇柱苣苔属植物叶插繁殖试验[J].南方农业学报,2012,43(3):356-359.
[11]杨平,陆婷,邱志敬.濒危植物秦岭石蝴蝶的叶插繁殖研究[J].北方园艺,2016(11):57-60.
[12]邱志敬,邹纯清,谭小龙,等.苦苣苔科植物的扦插繁育研究[J].北方园艺,2015(11):60-65.
[13]邓涛,何永艳,周太久,等.外源IBA对5种苦苣苔科植物扦插繁殖的影响[J].经济林研究,2013,31(3):68-71.
[14]唐文秀,黄仕训,隗红燕,等.石灰岩植物桂林唇柱苣苔叶插繁殖研究[J].西南农业学报,2009,22(5):1395-1399.
[15]刘伟,曹晓慧.吊石苣苔扦插繁殖研究[J].北方园艺,2010(2):116-118.
[16]卢永彬,黄俞松,许为斌,等.石山苣苔属(苦苣苔科)花型态演化及分类学意义[J].广西植物,2017,37(10):1227-1239.
[17]吴昊天,徐双双,杨世勇,等.岩溶穴居性陆氏石山苣苔群落物种组成与生态为分析研究[J].广西植物,2017,10(37):1240-1249.