HOX转录反义RNA在乳腺癌中的表达及意义
摘要
目的探讨乳腺癌组织中HOX转录反义RNA(HOTAIR)和其调控基因HOXD10的表达情况及HOTAIR基因的甲基化情况。方法对45对乳腺癌组织、癌旁组织、前哨淋巴结组织、腋窝淋巴结组织进行研究,采用实时荧光定量PCR法测定HOTAIR和HOXD10的表达情况,甲基化特异性聚合酶链反应测定HOTAIR非甲基化情况。结果乳腺癌组织中HOTAIR相对表达量(10. 22±3. 65)显著高于癌旁组织(5. 43±2. 19,P<0. 05),但两组HOXD10相对表达量比较差异无统计学意义(P>0. 05)。乳腺癌组织中HOTAIR基因非甲基化率(88. 9%)显著高于癌旁组织(44. 4%,P<0. 05)。乳腺癌组织中HOTAIR基因与Ki-67和孕激素受体呈正相关(r=0. 810、0. 623,P均<0. 05)。前哨淋巴结组织中HOTAIR相对表达量(9. 27±1. 19)显著高于腋窝淋巴结组织(6. 86±1. 24,t=3. 397,P<0. 05)。前哨淋巴结组织中HOTAIR与Ki-67和淋巴结转移率呈正相关(r=0. 803、0. 687,P均<0. 05)。结论乳腺癌组织和前哨淋巴结组织中HOTAIR基因表达水平升高,乳腺癌组织中HOTAIR与Ki-67和孕激素受体呈正相关,其调控基因HOXD10表达量无明显变化,HOTAIR基因非甲基化水平升高。
引文
1夏明,黄卫人,蔡志明,等.长链非编码RNA HOTAIR对肾癌细胞增殖和凋亡的影响[J].安徽医科大学学报,2015;50(8):1095-9.
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4洪伟,陈世良,陈祥锦,等.同源盒基因转录反义RNA调控乳腺癌细胞增殖和转移研究[J].中国临床药理学杂志,2015;31(17):1740-1.
5 Xue X,Yang YA,Zhang A,et al.LncRNA HOTAIR enhances ER signaling and confers tamoxifen resistance in breast cancer[J].Oncogene,2016;35(21):2746-55.
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7崔广晖,崔明威,李宇航,等.长链非编码RNA HOTAIR对食管鳞癌细胞EC9706恶性生物学行为的影响[J].中华实验外科杂志,2014;31(8):1657-8.
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9 Bhan A,Hussain I,Ansari KI,et al.Bisphenol-A and diethylstilbestrol exposure induces the expression of breast cancer associated long noncoding RNA HOTAIR in vitro and in vivo[J].J Steroid Biochem Mol Biol,2014;141:160-70.
10 Srensen KP,Thomassen M,Tan Q,et al.Long non-coding RNA HO-TAIR is an independent prognostic marker of metastasis in estrogen receptor-positive primary breast cancer[J].Breast Cancer Res Treat,2013;142(3):529-36.
11张开炯,张毅,罗正莲,等.血浆长链非编码RNA HOTAIR是乳腺癌诊断的潜在生物标志物[J].南方医科大学学报,2016;36(4):488-92.
12杨韬,李俊堂,王丽娟,等.干涉lncRNAsHOTAIR对人乳腺癌细胞MDA-MB-231的影响[J].细胞与分子免疫学杂志,2012;28(1):97-8.
13 Lu L,Zhu G,Zhang C,et al.Association of large noncoding RNA HO-TAIR expression and its downstream intergenic CpG island methylation with survival in breast cancer[J].Breast Cancer Res Treat,2012;136(3):875-83.
14 Sato K,Miyashita M,Ishida T,et al.Prognostic significance of the progesterone receptor status in Ki67-high and-low Luminal B-like HER2-negative breast cancers[J].Breast Cancer,2016;23(2):310-7.
15 Yagi T,Inoue N,Yanai A,et al.Prognostic significance of geminin expression levels in Ki67-high subset of estrogen receptor-positive and HER2-negative breast cancers[J].Breast Cancer,2016;23(2):224-30.
2 Wang Y,Wang H,Song T,et al.HOTAIR is a potential target for the treatment of cisplatin resistant ovarian cancer[J].Mol Med Rep,2015;12(2):2211-6.
3 Yan R,Cao J,Song C,et al.Polymorphisms in lncRNA HOTAIR and susceptibility to breast cancer in a Chinese population[J].Cancer Epidemiol,2015;39(6):978-85.
4洪伟,陈世良,陈祥锦,等.同源盒基因转录反义RNA调控乳腺癌细胞增殖和转移研究[J].中国临床药理学杂志,2015;31(17):1740-1.
5 Xue X,Yang YA,Zhang A,et al.LncRNA HOTAIR enhances ER signaling and confers tamoxifen resistance in breast cancer[J].Oncogene,2016;35(21):2746-55.
6 Kim HJ,Lee DW,Yim GW,et al.Long non-coding RNA HOTAIR is associated with human cervical cancer progression[J].Int J Oncol,2015;46(2):521-30.
7崔广晖,崔明威,李宇航,等.长链非编码RNA HOTAIR对食管鳞癌细胞EC9706恶性生物学行为的影响[J].中华实验外科杂志,2014;31(8):1657-8.
8 Zhang L,Song X,Wang X,et al.Circulating DNA of HOTAIR in serum is a novel biomarker for breast cancer[J].Breast Cancer Res Treat,2015;152(1):199-208.
9 Bhan A,Hussain I,Ansari KI,et al.Bisphenol-A and diethylstilbestrol exposure induces the expression of breast cancer associated long noncoding RNA HOTAIR in vitro and in vivo[J].J Steroid Biochem Mol Biol,2014;141:160-70.
10 Srensen KP,Thomassen M,Tan Q,et al.Long non-coding RNA HO-TAIR is an independent prognostic marker of metastasis in estrogen receptor-positive primary breast cancer[J].Breast Cancer Res Treat,2013;142(3):529-36.
11张开炯,张毅,罗正莲,等.血浆长链非编码RNA HOTAIR是乳腺癌诊断的潜在生物标志物[J].南方医科大学学报,2016;36(4):488-92.
12杨韬,李俊堂,王丽娟,等.干涉lncRNAsHOTAIR对人乳腺癌细胞MDA-MB-231的影响[J].细胞与分子免疫学杂志,2012;28(1):97-8.
13 Lu L,Zhu G,Zhang C,et al.Association of large noncoding RNA HO-TAIR expression and its downstream intergenic CpG island methylation with survival in breast cancer[J].Breast Cancer Res Treat,2012;136(3):875-83.
14 Sato K,Miyashita M,Ishida T,et al.Prognostic significance of the progesterone receptor status in Ki67-high and-low Luminal B-like HER2-negative breast cancers[J].Breast Cancer,2016;23(2):310-7.
15 Yagi T,Inoue N,Yanai A,et al.Prognostic significance of geminin expression levels in Ki67-high subset of estrogen receptor-positive and HER2-negative breast cancers[J].Breast Cancer,2016;23(2):224-30.