细胞分裂周期蛋白-6、同源异型盒基因-5蛋白表达水平与乳腺癌患者组织学分级的关联性分析
|
摘要
目的:探讨同源异型盒基因-5(HOXA5)、细胞分裂周期蛋白-6(CDC6)蛋白表达水平与乳腺癌患者组织学分级的关联性。方法:选取乳腺癌患者119例与癌前病变119例患者为研究对象。所有乳腺癌患者均采取手术治疗,并于术中切取病变组织,用免疫组化法测定HOXA5及CDC6阳性表达情况。统计乳腺癌组与癌前病变组、乳腺癌组不同组织分化程度患者间HOXA5及CDC6阳性表达情况。结果:与癌前病变组比较,乳腺癌组CDC6(74.79%vs 19.33%,P<0.05))和HOXA5(68.07%vs 26.05%,P<0.05)阳性表达率显著增加;不同组织分化程度乳腺癌患者间HOXA5及CDC6阳性表达率存在显著差异(P<0.05),且高分化患者CDC6阳性率(53.85%)低于中分化(79.59%)及低分化患者(93.55%),高分化患者HOXA5阳性率(51.28%)低于低分化患者(87.10%)(P<0.05)。结论:乳腺癌患者肿瘤组织中HOXA5及CDC6过表达与乳腺癌组织学分级密切相关。
Objective:To investigate the relationship between the expression levels of homeobox gene-5(HOXA5)and cell division cyclin-6(CDC6)and histological grade in breast cancer patients.Methods:119breast cancer patients and 119 patients with precancerous lesions were selected as breast cancer patients.All patients with breast cancer were treated with surgery,and the diseased tissue was harvested during operation.The positive expression of HOXA5 and CDC6was determined by immunohistochemistry.The positive expressions of HOXA5 and CDC6in patients with different degrees of tissue differentiation in breast cancer group and precancerous lesion group and breast cancer group were analyzed.Results:The positive expression rates of CDC6(74.79%)and HOXA5(68.07%)in breast cancer group were significantly higher than those in precancerous lesions(19.33%,26.05%)(P<0.05).HOXA5 and CDC6were positive among breast cancer patients with different degrees of tissue differentiation.There was a significant difference in expression rate(P<0.05),and the positive rate of CDC6 in highly differentiated patients(53.85%)was lower than that in moderate differentiation(79.59%)and poorly differentiated(93.55%).The positive rate of HOXA5 in highly differentiated patients(51.28%)was lower than that in poor differentiation(87.10%)(P<0.05).Conclusion:The overexpression of HOXA5 and CDC6in breast cancer patients is closely related to the histological grade of breast cancer.
Objective:To investigate the relationship between the expression levels of homeobox gene-5(HOXA5)and cell division cyclin-6(CDC6)and histological grade in breast cancer patients.Methods:119breast cancer patients and 119 patients with precancerous lesions were selected as breast cancer patients.All patients with breast cancer were treated with surgery,and the diseased tissue was harvested during operation.The positive expression of HOXA5 and CDC6was determined by immunohistochemistry.The positive expressions of HOXA5 and CDC6in patients with different degrees of tissue differentiation in breast cancer group and precancerous lesion group and breast cancer group were analyzed.Results:The positive expression rates of CDC6(74.79%)and HOXA5(68.07%)in breast cancer group were significantly higher than those in precancerous lesions(19.33%,26.05%)(P<0.05).HOXA5 and CDC6were positive among breast cancer patients with different degrees of tissue differentiation.There was a significant difference in expression rate(P<0.05),and the positive rate of CDC6 in highly differentiated patients(53.85%)was lower than that in moderate differentiation(79.59%)and poorly differentiated(93.55%).The positive rate of HOXA5 in highly differentiated patients(51.28%)was lower than that in poor differentiation(87.10%)(P<0.05).Conclusion:The overexpression of HOXA5 and CDC6in breast cancer patients is closely related to the histological grade of breast cancer.
引文
[1]董娇娇,吕志栋,李福年,等.同源异型核基因1诱导乳腺癌细胞发生上皮-间充质转化对三苯氧胺耐药的研究[J].中华实验外科杂志,2018,35(6):1002-1004.
[2]王文娟,吕志栋,张琳,等.同源异型核基因1过表达诱导上皮-间充质转化促进乳腺癌细胞转移能力的研究[J].中华实验外科杂志,2017,34(6):959-962.
[3]马杜丰,周家德.多种影像学联合检查诊断早期乳腺癌的临床分析[J].中国现代普通外科进展,2016,19(7):564-566.
[4]吕可君.乳腺癌组织中CDC6及HOXA5蛋白的表达及临床意义[J].现代实用医学,2017,29(9):1150-1153.
[5]张立美,战军,张宏权,等.转录因子HOXA5在乳腺癌细胞中的下游信号通路[J].解剖学报,2015,46(5):634-640.
[6]伦淑敏.HOXA5基因真核表达质粒的构建及在乳腺癌细胞中的功能研究[J].天津医科大学学报,2014,20(5):337-341.
[7]张慧,赵瑞珂,顾国浩,等.乳腺癌患者部分外周血生化指标的变化及其意义[J].检验医学与临床,2016,13(15):2153-2155.
[8]范光磊,万仁明,彭鸣亚,等.癌胚抗原、糖类抗原15-3及骨转换生化标志物检测对乳腺癌骨转移的诊断意义[J].中华核医学与分子影像杂志,2013,33(3):203-206.
[9]郭红艳,孙晓杰,李淑艳,等.蛋白激酶SGK3与乳腺癌临床病理相关性研究[J].现代预防医学,2016,43(6):1142-1145.
[10]齐攀,金艳,曹兴玥,等.CDC6蛋白在乳腺癌中表达及与病理指标的关系[J].中国现代药物应用,2015,9(16):37-39.
[11]耿强,钱晓龙,付丽.乳腺癌组织CDC6表达及其临床意义的研究[J].中华肿瘤防治杂志,2014,21(11):836-840.
[12]张立美,战军,张宏权,等.转录因子HOXA5在乳腺癌细胞中的下游信号通路研究[J].中国民康医学,2015,27(14):156-156.
[13]叶柳青,丁金旺,张敏,等.同源盒基因转录反义RNA(HOTAIR)在乳腺癌临床诊疗中的研究进展[J].中华全科医学,2018,16(2):286-290.
[2]王文娟,吕志栋,张琳,等.同源异型核基因1过表达诱导上皮-间充质转化促进乳腺癌细胞转移能力的研究[J].中华实验外科杂志,2017,34(6):959-962.
[3]马杜丰,周家德.多种影像学联合检查诊断早期乳腺癌的临床分析[J].中国现代普通外科进展,2016,19(7):564-566.
[4]吕可君.乳腺癌组织中CDC6及HOXA5蛋白的表达及临床意义[J].现代实用医学,2017,29(9):1150-1153.
[5]张立美,战军,张宏权,等.转录因子HOXA5在乳腺癌细胞中的下游信号通路[J].解剖学报,2015,46(5):634-640.
[6]伦淑敏.HOXA5基因真核表达质粒的构建及在乳腺癌细胞中的功能研究[J].天津医科大学学报,2014,20(5):337-341.
[7]张慧,赵瑞珂,顾国浩,等.乳腺癌患者部分外周血生化指标的变化及其意义[J].检验医学与临床,2016,13(15):2153-2155.
[8]范光磊,万仁明,彭鸣亚,等.癌胚抗原、糖类抗原15-3及骨转换生化标志物检测对乳腺癌骨转移的诊断意义[J].中华核医学与分子影像杂志,2013,33(3):203-206.
[9]郭红艳,孙晓杰,李淑艳,等.蛋白激酶SGK3与乳腺癌临床病理相关性研究[J].现代预防医学,2016,43(6):1142-1145.
[10]齐攀,金艳,曹兴玥,等.CDC6蛋白在乳腺癌中表达及与病理指标的关系[J].中国现代药物应用,2015,9(16):37-39.
[11]耿强,钱晓龙,付丽.乳腺癌组织CDC6表达及其临床意义的研究[J].中华肿瘤防治杂志,2014,21(11):836-840.
[12]张立美,战军,张宏权,等.转录因子HOXA5在乳腺癌细胞中的下游信号通路研究[J].中国民康医学,2015,27(14):156-156.
[13]叶柳青,丁金旺,张敏,等.同源盒基因转录反义RNA(HOTAIR)在乳腺癌临床诊疗中的研究进展[J].中华全科医学,2018,16(2):286-290.