饲养层细胞作用机制研究进展
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摘要
饲养层细胞法被广泛应用于干细胞及其他哺乳动物细胞的体外培养,通过分泌生长因子和物理支持可以一定程度模拟细胞增殖的体内环境。根据培养目的的不同,可以有选择性的挑选饲养层细胞种类、处理方法,或通过基因编辑对饲养层细胞进行改良,从而达到最佳的培养效果。
Feeder cells have been widely used as an assistant method for the in vitro growth of mammal cells.It can simulate the condition of in vivo culture to a certain extent by the secretion of growth factors and the physical support. According to different culture purposes,we may choose specific feeder cells and dealing methods or modify feeder cells through gene editing to obtain the best culture results.
Feeder cells have been widely used as an assistant method for the in vitro growth of mammal cells.It can simulate the condition of in vivo culture to a certain extent by the secretion of growth factors and the physical support. According to different culture purposes,we may choose specific feeder cells and dealing methods or modify feeder cells through gene editing to obtain the best culture results.
引文
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[23] Abdal Dayem A,Lee SY,Choi H,et al.The impact of adhesion molecules on the in vitro culture and differentia-tion of stem cells[J].Biotechnol J.doi:10.1022/biot.201700575.
[2] Ghasemi-Dehkordi P,Allahbakhshian-Farsani M,Abdian N,et al.Comparison between the cultures of human induced pluripotent stem cells (hiPSCs) on feeder-and serum-free system (Matrigel matrix),MEF and HDF feeder cell lines[J].J Cell Commun Signal,2015,9:233-246.
[3] Chugh RM,Chaturvedi M,Yerneni LK.An evaluation of the choice of feeder cell growth arrest for the production of cultured epidermis[J].Burns,2015,41:1788-1795.
[4] Le-Bel G,Giasson CJ,Deschambeault A,et al.The presence of a feeder layer improves human corneal endothelial cell proliferation by altering the expression of the transcription factors Sp1 and NFI[J].Exp Eye Res,2018,176:161-173.
[5] Sevilla A,Forero E,Zimmer M,et al.Derivation and characterization of the NYSCFe003-A human embryonic stem cell line[J].Stem Cell Res,2017,25:217-220.
[6] Tan X,Dai Q,Guo T,et al.Efficient generation of transgene- and feeder-free induced pluripotent stem cells from human dental mesenchymal stem cells and their chemically defined differentiation into cardiomyocytes[J].Biochem Biophys Res Commun,2018,495:2490-2497.
[7] Hansen M,Varga E,Aarts C,et al.Efficient production of erythroid,megakaryocytic and myeloid cells,using single cell-derived iPSC colony differentiation[J].Stem Cell Res,2018,29:232-244.
[8] Zeng Z,Xiang S,Xue W,et al.The cell death and DNA damages caused by the Tet-On regulating HSV-tk/GCV suicide gene system in MCF-7 cells[J].Biomed Pharmacother,2014,68:887-892.
[9] Llames S,García-Pérez E,Meana á,et al.Feeder layer cell actions and applications[J].Tissue Eng Part B Rev.2015,21:345-353.
[10] Browning LM,Xu XN.Electric pulses to prepare feeder cells for sustaining and culturing of undifferentiated embryonic stem cells[J].Biotechnol J,2010,5:588-595.
[11] Alkhilaiwi F,Wang L,Zhou D,et al.Long-term expansion of primary equine keratinocytes that maintain the ability to differentiate into stratified epidermis[J].Stem Cell Res Ther,2018,9;181-193.
[12] Horie M,Ito A,Maki T,et al.Magnetically labeled feeder system for mouse pluripotent stem cell culture[J].J Biosci Bioeng,2015,119:614-616.
[13] Yu G,Okawa H,Okita K,et al.Gingival fibroblasts as autologous feeders for induced pluripotent stem cells[J].J Dent Res,2015,95:110-118.
[14] Zhang R,Mjoseng HK,Hoeve MA,et al.A thermoresponsive and chemically defined hydrogel for long-term culture of human embryonic stem cells[J].Nat Commun,2013,4:1335-1345.
[15] Guo R,Ye X,Yang J,et al.Feeders facilitate telomere maintenance and chromosomal stability of embryonic stem cells[J].Nat Commun,2018,9:2620-2636.
[16] Li D,Wang L,Hou J,et al.Optimized approaches for generation of integration-free iPSCs from human urine-derived cells with small molecules and autologous feeder[J].Stem Cell Reports,2016,6:717-728.
[17] Kitchen JR,Cysyk RL.Synthesis and release of hyaluronic acid by Swiss 3T3 fibroblasts[J].Biochem J,1995,309:649-656.
[18] Sevilla A,Forero E,Zimmer M,et al.Derivation and characterization of the NIH registry human stem cell line NYSCF101 under defined feeder-free conditions[J].Stem Cell Res,2018,29:197-201.
[19] Wattanapanitch M,Damkham N,Potirat P,et al.One-step genetic correction of hemoglobin E/beta-thalassemia patient-derived iPSCs by the CRISPR/Cas9 system[J].Stem Cell Res Ther,2018,9:46-57.
[20] Chapman S,Liu X,Meyers C,et al.Human keratino-cytes are efficiently immortalized by a Rho kinase inhibitor[J].J Clin Invest,2010,120:2619-2626.
[21] Zhou Q,Duan H,Wang Y,et al.ROCK inhibitor Y-27632 increases the cloning efficiency of limbal stem/progenitor cells by improving their adherence and ROS-scavenging capacity[J].Tissue Eng Part C Methods,2013,19:531-537.
[22] Zhao Z,Ma Y,Chen Z,et al.Effects of feeder cells on dopaminergic differentiation of human embryonic stem cells[J].Front Cell Neurosci,2016,10:291-307.
[23] Abdal Dayem A,Lee SY,Choi H,et al.The impact of adhesion molecules on the in vitro culture and differentia-tion of stem cells[J].Biotechnol J.doi:10.1022/biot.201700575.