醋酸对草莓果实成熟影响及其生理机制
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摘要
【目的】为了研究有机酸对草莓果实成熟的影响及开发成熟调控技术。【方法】首先分析草莓果实发育期间pH值的变化;其次以清水为对照,用0.5%的醋酸对草莓大绿果进行喷施处理并结合酶联免疫法检测处理后草莓果实中内源激素水平的变化。【结果】结果表明,随着草莓果实的着色成熟,pH逐渐增加。同对照相比,0.5%醋酸处理显著抑制草莓果实的成熟;除GA_3外,0.5%醋酸处理显著促进了多种激素的积累,如IAA、IPA、GA4、ZR、MeJA、ABA和BR,尤其ABA和IAA成倍数增加。【结论】醋酸可有效延缓草莓果实的成熟,这种作用与ABA和IAA密切相关。
【Objective】The study is focused on the effect of organic acids on ripening of strawberry fruit and the development of ripening-regulation techniques.【Methods】0.5% acetic acid was applied to spray green fruit,the pH value during strawberry fruit development and levels of endogenous hormones were detected in the treated fruits by enzyme-linked immune sorbent assay(ELISA).【Results】The results showed that the pH values gradually increased with red-coloring and ripening of strawberry fruit.Compared with the control,0.5% acetic acid treatment significantly inhibited the ripening;0.5% acetic acid significantly promoted the accumulation of various hormones including IAA,IPA,GA4,ZR,MeJA,ABA,or BR with the exception of GA_3,ABA or IAA increased in multiplcation.【Conclusion】Acetic acid could effectively delay strawberry fruit ripening,which was closely correlated with ABA and IAA.
【Objective】The study is focused on the effect of organic acids on ripening of strawberry fruit and the development of ripening-regulation techniques.【Methods】0.5% acetic acid was applied to spray green fruit,the pH value during strawberry fruit development and levels of endogenous hormones were detected in the treated fruits by enzyme-linked immune sorbent assay(ELISA).【Results】The results showed that the pH values gradually increased with red-coloring and ripening of strawberry fruit.Compared with the control,0.5% acetic acid treatment significantly inhibited the ripening;0.5% acetic acid significantly promoted the accumulation of various hormones including IAA,IPA,GA4,ZR,MeJA,ABA,or BR with the exception of GA_3,ABA or IAA increased in multiplcation.【Conclusion】Acetic acid could effectively delay strawberry fruit ripening,which was closely correlated with ABA and IAA.
引文
[1]Alexander L,Grierson D.Ethylene biosynthesis and action in tomato:a model for climacteric fruit ripening[J].Journal of Experimental Botany,2002,53:2039-2055
[2]Chunli Li,Haifeng Jia,Yemao Chai,Yuanyue Shen.Abscisic acid perception and signaling transduction in strawberry:Amodel for non-climacteric fruit ripening[J].Plant Signaling&Amp;Behavior,2011,6:1-4
[3]Chai Y M,Jia H F,Li CL,et al.FaPYR1is involved in strawberry fruit ripening[J].Plant Growth Regulation,2013,69:63-69
[4]Concha C M,Figueroa N E,Poblete L A,et al.Methyl jasmonate treatment induces changes in fruit ripening by modifying the expression of several ripening genes in Fragaria chiloensis fruit[J].Plant Physiol Biochem,2013,70(1):433-444
[5]Catharina Merchante,Jose M Alonso,Anna N Stepanova.Ethylene signaling:simple ligand,complex regulation[J].Current Opinion in Plant Biology,2013,16(5):554-560
[6]Symons G M,Chua Y J,Ross J J,Quittenden L J,Davies NW,Reid J B.Hormonal changes during non-climacteric ripening in strawberry[J].Journal of Experimental Botany,2012,63(13):4741-4750
[7]Catharina M,Vallarino J G,Sonia O,et al.Ethylene is involved in strawberry fruit ripening in an organ-specific manner[J].Journal of Experimental Botany,2013,64:4421-4439
[8]Guo J,Wang S,Yu X,et al.Polyamines Regulate Strawberry Fruit Ripening by Abscisic Acid,Auxin,and Ethylene[J].Plant Physiol,2018,177:339-351
[9]Jia H,Wang Y,Sun M,et al.Sucrose functions as a signal involved in the regulation of strawberry fruit development and ripening[J].New Phytologist,2013,198:453-465
[10]Jia H F,Dong L,Sun J H,et al.Type 2Cprotein phosphatase ABI1is a negative regulator of strawberry fruit ripening[J].Journal of Experimental Botany,2013,64:1677-1687
[11]李青云,葛会波,张广华,等.日光温室草莓果实生长发育过程中糖、酸和V-C变化动态的研究[J].河北农业大学学报,2002,25:46-48
[12]Given K,Venis M A,Gierson D.Hormonal regulation of ripening in the strawberry,a non-climacteric fruit[J].Planta,1988,174:402-406
[13]Manning K.Changes in gene expression during strawberry fruit ripening and their regulation by auxin[J].Planta,1994,194:62-68
[14]Davies C,Boss P K.Treatment of Grape Berries,a nonclimacteric fruit with a synthetic auxin,retards ripening and alters the expression of developmentally regulated genes[J].Plant Physiology,1997,115:1155-1161
[2]Chunli Li,Haifeng Jia,Yemao Chai,Yuanyue Shen.Abscisic acid perception and signaling transduction in strawberry:Amodel for non-climacteric fruit ripening[J].Plant Signaling&Amp;Behavior,2011,6:1-4
[3]Chai Y M,Jia H F,Li CL,et al.FaPYR1is involved in strawberry fruit ripening[J].Plant Growth Regulation,2013,69:63-69
[4]Concha C M,Figueroa N E,Poblete L A,et al.Methyl jasmonate treatment induces changes in fruit ripening by modifying the expression of several ripening genes in Fragaria chiloensis fruit[J].Plant Physiol Biochem,2013,70(1):433-444
[5]Catharina Merchante,Jose M Alonso,Anna N Stepanova.Ethylene signaling:simple ligand,complex regulation[J].Current Opinion in Plant Biology,2013,16(5):554-560
[6]Symons G M,Chua Y J,Ross J J,Quittenden L J,Davies NW,Reid J B.Hormonal changes during non-climacteric ripening in strawberry[J].Journal of Experimental Botany,2012,63(13):4741-4750
[7]Catharina M,Vallarino J G,Sonia O,et al.Ethylene is involved in strawberry fruit ripening in an organ-specific manner[J].Journal of Experimental Botany,2013,64:4421-4439
[8]Guo J,Wang S,Yu X,et al.Polyamines Regulate Strawberry Fruit Ripening by Abscisic Acid,Auxin,and Ethylene[J].Plant Physiol,2018,177:339-351
[9]Jia H,Wang Y,Sun M,et al.Sucrose functions as a signal involved in the regulation of strawberry fruit development and ripening[J].New Phytologist,2013,198:453-465
[10]Jia H F,Dong L,Sun J H,et al.Type 2Cprotein phosphatase ABI1is a negative regulator of strawberry fruit ripening[J].Journal of Experimental Botany,2013,64:1677-1687
[11]李青云,葛会波,张广华,等.日光温室草莓果实生长发育过程中糖、酸和V-C变化动态的研究[J].河北农业大学学报,2002,25:46-48
[12]Given K,Venis M A,Gierson D.Hormonal regulation of ripening in the strawberry,a non-climacteric fruit[J].Planta,1988,174:402-406
[13]Manning K.Changes in gene expression during strawberry fruit ripening and their regulation by auxin[J].Planta,1994,194:62-68
[14]Davies C,Boss P K.Treatment of Grape Berries,a nonclimacteric fruit with a synthetic auxin,retards ripening and alters the expression of developmentally regulated genes[J].Plant Physiology,1997,115:1155-1161