胞壁酸研究进展
|
摘要
胞壁酸是细菌细胞壁肽聚糖的组成成分,也是原核微生物细胞的生物标志化合物,常作为检测食品、空气、组织等细菌含量的重要指标。胞壁酸在临床医学、免疫佐剂活性、与植物凝集素的相互作用、睡眠促进因子等方面具有重要的生理功能。胞壁酸含量的检测方法多种多样,目前文献报道的方法有薄层层析法(TLC)、分光光度法、高效液相色谱法(HPLC)、气相色谱-质谱联用法(GC-MS)、自动氨基酸分析法、高效毛细管电泳法等,可根据样品处理方法的不同,以及试验条件的不同选用适当的检测方法。本文概述了胞壁酸的理化性质、生理功能应用和检测方法。
Muramic acid, the component of bacterial cell wall peptidoglycan, is the biomarker compounds of procaryote microbiology cell. It is often used as an important indicator for the bacteria content in food, air, and tissues. It has important physiological functions in clinical medicine, immune adjuvant activity, interaction with plant lectins, sleeppromoting factor and so on. The detection methods of muramic acid content are various. The main determination methods for muramic acid include thin-layer chromatography(TLC), spectrophotometry, high performance liquid chromatography(HPLC), gas chromatography-mass spectrography(GC-MS), automatic amino acid analysis, and high performance capillary electrophoresis. The detection methods could be selected according to the sample treatments and experimental conditions. In this review, the physical and chemical properties, physiological application, and detection methods for muramic acid were summarized.
Muramic acid, the component of bacterial cell wall peptidoglycan, is the biomarker compounds of procaryote microbiology cell. It is often used as an important indicator for the bacteria content in food, air, and tissues. It has important physiological functions in clinical medicine, immune adjuvant activity, interaction with plant lectins, sleeppromoting factor and so on. The detection methods of muramic acid content are various. The main determination methods for muramic acid include thin-layer chromatography(TLC), spectrophotometry, high performance liquid chromatography(HPLC), gas chromatography-mass spectrography(GC-MS), automatic amino acid analysis, and high performance capillary electrophoresis. The detection methods could be selected according to the sample treatments and experimental conditions. In this review, the physical and chemical properties, physiological application, and detection methods for muramic acid were summarized.
引文
[1]MATSUSHIMA Y,PARK J T.Stereospecific synthesis of 2-amino-3-O-(D-1′-carboxyethyl)-2-deoxyD-glucose(muramic acid)and related compounds[J].Journal of Organic Chemistry,1962,27(10):3581-3583.
[2]王秀丽.重金属复合污染对土壤环境微生物群落的影响机理研究[D].杭州:浙江大学,2002.
[3]楊永年.细菌细胞壁(中篇)[J].郑州大学学报(医学版),1964,2:36-52.
[4]MILLAR W N,JR C L.Evidence for muramic acid in soil[J].Canadian Journal of Microbiology,1970,16(5):299-304.
[5]PETERSON D,DAVID E,BERNLOHR R W.Determination of muramic acid,ornithine,and diaminopimelic acid during automatic amino acid analysis[J].Analytical Biochemistry,1970,33(2):238-243.
[6]HADZIJA O.A simple method for the quantitative determination of muramicacid[J].Analytical Biochemistry,1974,60(2):512-517.
[7]MORIARTY D J W.Improved method using muramic acid to estimate biomass of bacteria in sediments[J].Oecologia,1977,26(4):317-323.
[8]SEN Z,KARNOVSKY M L.Qualitative detection of muramic acid in normal mammalian tissues[J].Infection and Immunity,1984,43(3):937-941.
[9]CHRISTENSSON B,GILBART J,FOX A,et al Mass spectrometric quantitation of muramic acid,a bacterial cell wall component,in septic synovial fluids[J].Arthritis and Rheumatology,1989,32(10):1268-1272.
[10]MIELNICZUK Z,MIELNICZUK E,LARSSON LDetermination of muramic acid in organic dust by gas chromatography-mass spectrometry[J].Journal of Chromatography B Biomedical Applications,1995,670(1):167-172.
[11]MIELNICZUK Z,POGORZELSKA Z.Detection of microbial contamination of packaging for foodstuffs by gas chromatography-mass spectrometry method[J].Packaging Technology and Science,2002,15(1):47-51.
[12]LIANG C,TERIC B.Mass spectrometric characterization of amino sugar aldononitrile acetate derivatives used for isotope enrichment assessment of microbial residues[J].Soil Biology and Biochemistry,2010,42(6):904-909.
[13]INDORF C,DYCKMANS J,KHAN K S,et al Optimisation of amino sugar quantification by HPLCin soil and plant hydrolysates[J].Biology and Fertility of Soils,2011,47(4):387-396.
[14]ADHIKARI A,POPHAM D,SCHAFFER C,et al.Muramic acid in indoor air and dust:Relationship with home characteristics[J].Journal of Allergy and Clinical Immunology,2013,131(2):AB13.
[15]ZHANG B,YANG X,DRURY C F,et al.A midinfrared spectroscopy method to determine the glucosamine,galactosamine,and muramic acid concentrations in soil hydrolysates[J].Soil Science Society of America Journal,2013,77(3):842-849.
[16]李树新.胞壁酰二肽的神经免疫调节作用[J].中国药理学通报,1994,10(1):5-8.
[17]蒋新英.谷氨酸棒状杆菌菌渣中胞壁酸的提取、纯化及检测[D].济南:山东师范大学,2015.
[18]潘孝彰,翁心华,徐肇玥.胞壁酸抗体及其临床应用[J].国外医学(内科学分册),1982,9(4):178-179.
[19]王晶,张旭东,解宏图,等.现代土壤有机质研究中新的量化指标概述[J].应用生态学报,2003,14(10):1809-1812.
[20]ZHANG X D,AMELUNG W.Gas chromatographic determination of muramic acid,glucosamine,mannosamine,and galactosamine in soil[J].Soil Biol Biochem,1996,28(9):1201-1206.
[21]POOLE J A,DOOLEY G P,SAITO R,et al.Muramic acid,endotoxin,3-hydroxy fatty acids,and ergosterol content explain monocyte and epithelial cell inflammatory responses to agricultural dusts[J].Journal of Toxicology and Environmental Health Part A,2010,73(10):684-700.
[22]AYOUBA A,CHATELAIN C,ROUGEP.Legume lectins interact with muramic acid and N-acetylmuramicacid[J].FEBS Letters,1991,289(1):102-104.
[23]BOURNE Y,AYOUBA A,ROUGEP,et al.Interaction of a legume lectin with two components of the bacterial cell wall.A crystallographic study[J].Journal of Biological Chemistry,1994,269(13):9429-9435.
[24]骆成榆.胞壁酸抗体试验[J].国外医学参考资料(流行病学传染病学分册),1978(3):141-142.
[25]徐杰诚,张明珠.胞壁酰二肽(MDP)及其类似物的合成[J].化学学报,1995(5):509-515.
[26]王立.增强人体免疫力[J].开卷有益-求医问药,2015,15(9):22.
[27]KRUEGER M L.The composition of sleep-promoting factor isolated from human urine[J].Journal of Biological Chemistry,1982,257(4):1664-1669.
[28]OTTAVIANI E,FANTIN A M B,BOLOGNANI LMuramic acid as aglycoconjugate component in Mollusca Gastropoda[J].Comparative Biochemistry and Physiology Part B:Comparative Biochemistry,1990,96(4):627-632.
[29]于宏晓,赵曰利,徐海涛,等.氨基酸分析技术的研究进展[J].农产品加工(学刊),2011(3):44-47.
[30]张祝兰,林善,唐文力,等.红色诺卡氏菌细胞壁骨架的理化性质、化学成分及含量测定的研究[J].中国抗生素杂志,2002(9):532-534.
[31]张惟杰.糖复合物生化研究技术[M].2版.杭州:浙江大学出版社,1999:9-72.
[32]苏华,张友逊,李荣德.应用色-质联用法证实沙眼衣原体中胞壁酸的存在[J].科学通报,1984(15):950-952.
[33]陆德培,黄克武,李荣春.糖的高效液相色谱分析研究---Ⅰ中性糖、氨基糖和双糖的分析[J].生物化学与生物物理学报,1982(5):73-78.
[34]李敬慈.高效液相色谱法测糖[J].河北大学学报(自然科学版),1997(4):89-94.
[35]ZELLES L.The simultaneous determination of muramic acid and glucosamine in soil by high-performance liquid chromatography with precolumn fluorescence derivatization[J].Biology and Fertility of Soils,1988,6(2):125-130.
[36]FAZIO S D,MAYBERRY W R,WHITE D CMuramic acid assay in sediments[J].Applied and Environmental Microbiology,1979,38(2):349-350.
[37]TAYLOR K A C C.A simple colorimetric assay for muramic acid and lactic acid[J].Applied Biochemistry and Biotechnology,1996,56(1):49-58.
[2]王秀丽.重金属复合污染对土壤环境微生物群落的影响机理研究[D].杭州:浙江大学,2002.
[3]楊永年.细菌细胞壁(中篇)[J].郑州大学学报(医学版),1964,2:36-52.
[4]MILLAR W N,JR C L.Evidence for muramic acid in soil[J].Canadian Journal of Microbiology,1970,16(5):299-304.
[5]PETERSON D,DAVID E,BERNLOHR R W.Determination of muramic acid,ornithine,and diaminopimelic acid during automatic amino acid analysis[J].Analytical Biochemistry,1970,33(2):238-243.
[6]HADZIJA O.A simple method for the quantitative determination of muramicacid[J].Analytical Biochemistry,1974,60(2):512-517.
[7]MORIARTY D J W.Improved method using muramic acid to estimate biomass of bacteria in sediments[J].Oecologia,1977,26(4):317-323.
[8]SEN Z,KARNOVSKY M L.Qualitative detection of muramic acid in normal mammalian tissues[J].Infection and Immunity,1984,43(3):937-941.
[9]CHRISTENSSON B,GILBART J,FOX A,et al Mass spectrometric quantitation of muramic acid,a bacterial cell wall component,in septic synovial fluids[J].Arthritis and Rheumatology,1989,32(10):1268-1272.
[10]MIELNICZUK Z,MIELNICZUK E,LARSSON LDetermination of muramic acid in organic dust by gas chromatography-mass spectrometry[J].Journal of Chromatography B Biomedical Applications,1995,670(1):167-172.
[11]MIELNICZUK Z,POGORZELSKA Z.Detection of microbial contamination of packaging for foodstuffs by gas chromatography-mass spectrometry method[J].Packaging Technology and Science,2002,15(1):47-51.
[12]LIANG C,TERIC B.Mass spectrometric characterization of amino sugar aldononitrile acetate derivatives used for isotope enrichment assessment of microbial residues[J].Soil Biology and Biochemistry,2010,42(6):904-909.
[13]INDORF C,DYCKMANS J,KHAN K S,et al Optimisation of amino sugar quantification by HPLCin soil and plant hydrolysates[J].Biology and Fertility of Soils,2011,47(4):387-396.
[14]ADHIKARI A,POPHAM D,SCHAFFER C,et al.Muramic acid in indoor air and dust:Relationship with home characteristics[J].Journal of Allergy and Clinical Immunology,2013,131(2):AB13.
[15]ZHANG B,YANG X,DRURY C F,et al.A midinfrared spectroscopy method to determine the glucosamine,galactosamine,and muramic acid concentrations in soil hydrolysates[J].Soil Science Society of America Journal,2013,77(3):842-849.
[16]李树新.胞壁酰二肽的神经免疫调节作用[J].中国药理学通报,1994,10(1):5-8.
[17]蒋新英.谷氨酸棒状杆菌菌渣中胞壁酸的提取、纯化及检测[D].济南:山东师范大学,2015.
[18]潘孝彰,翁心华,徐肇玥.胞壁酸抗体及其临床应用[J].国外医学(内科学分册),1982,9(4):178-179.
[19]王晶,张旭东,解宏图,等.现代土壤有机质研究中新的量化指标概述[J].应用生态学报,2003,14(10):1809-1812.
[20]ZHANG X D,AMELUNG W.Gas chromatographic determination of muramic acid,glucosamine,mannosamine,and galactosamine in soil[J].Soil Biol Biochem,1996,28(9):1201-1206.
[21]POOLE J A,DOOLEY G P,SAITO R,et al.Muramic acid,endotoxin,3-hydroxy fatty acids,and ergosterol content explain monocyte and epithelial cell inflammatory responses to agricultural dusts[J].Journal of Toxicology and Environmental Health Part A,2010,73(10):684-700.
[22]AYOUBA A,CHATELAIN C,ROUGEP.Legume lectins interact with muramic acid and N-acetylmuramicacid[J].FEBS Letters,1991,289(1):102-104.
[23]BOURNE Y,AYOUBA A,ROUGEP,et al.Interaction of a legume lectin with two components of the bacterial cell wall.A crystallographic study[J].Journal of Biological Chemistry,1994,269(13):9429-9435.
[24]骆成榆.胞壁酸抗体试验[J].国外医学参考资料(流行病学传染病学分册),1978(3):141-142.
[25]徐杰诚,张明珠.胞壁酰二肽(MDP)及其类似物的合成[J].化学学报,1995(5):509-515.
[26]王立.增强人体免疫力[J].开卷有益-求医问药,2015,15(9):22.
[27]KRUEGER M L.The composition of sleep-promoting factor isolated from human urine[J].Journal of Biological Chemistry,1982,257(4):1664-1669.
[28]OTTAVIANI E,FANTIN A M B,BOLOGNANI LMuramic acid as aglycoconjugate component in Mollusca Gastropoda[J].Comparative Biochemistry and Physiology Part B:Comparative Biochemistry,1990,96(4):627-632.
[29]于宏晓,赵曰利,徐海涛,等.氨基酸分析技术的研究进展[J].农产品加工(学刊),2011(3):44-47.
[30]张祝兰,林善,唐文力,等.红色诺卡氏菌细胞壁骨架的理化性质、化学成分及含量测定的研究[J].中国抗生素杂志,2002(9):532-534.
[31]张惟杰.糖复合物生化研究技术[M].2版.杭州:浙江大学出版社,1999:9-72.
[32]苏华,张友逊,李荣德.应用色-质联用法证实沙眼衣原体中胞壁酸的存在[J].科学通报,1984(15):950-952.
[33]陆德培,黄克武,李荣春.糖的高效液相色谱分析研究---Ⅰ中性糖、氨基糖和双糖的分析[J].生物化学与生物物理学报,1982(5):73-78.
[34]李敬慈.高效液相色谱法测糖[J].河北大学学报(自然科学版),1997(4):89-94.
[35]ZELLES L.The simultaneous determination of muramic acid and glucosamine in soil by high-performance liquid chromatography with precolumn fluorescence derivatization[J].Biology and Fertility of Soils,1988,6(2):125-130.
[36]FAZIO S D,MAYBERRY W R,WHITE D CMuramic acid assay in sediments[J].Applied and Environmental Microbiology,1979,38(2):349-350.
[37]TAYLOR K A C C.A simple colorimetric assay for muramic acid and lactic acid[J].Applied Biochemistry and Biotechnology,1996,56(1):49-58.