玉屏风散合四物汤对慢性阻塞性肺疾病气道重塑的影响
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摘要
目的:观察玉屏风散合四物汤对气道重塑的影响。方法:将SPF级雄性Wistar大鼠利用随机数字表分为正常组,模型组,玉屏风散合四物汤低、中、高剂量组,每组10只。慢性阻塞性肺疾病(COPD)大鼠模型采用气管滴注脂多糖加烟熏的方法构建。收集大鼠肺组织匀浆进行分泌型免疫球蛋白A(SIg A)、分泌成分(SC)、转化生长因子β_1(TGF-β_1)、血管内皮生长因子(VEGF)的检测。结果:与正常组相比,模型组SIg A、SC、TGF-β_1、VEGF含量均显著升高,差异有统计学意义(P<0.05);与模型组相比,玉屏风散合四物汤低剂量组SIg A、SC、TGF-β_1、VEGF含量显著降低,差异有统计学意义(P<0.01);中剂量组对TGF-β_1作用不明显,对其余3种指标效果较好(P<0.05,P<0.01),高剂量组只对VEGF有作用(P<0.01)。结论:玉屏风散合四物汤能有效降低COPD大鼠SIg A、SC、TGF-β_1、VEGF的含量,这可能是其治疗COPD的重要机制之一。
Objective:To investigate the effects of YuPingFeng powder joined with SiWu decoction on airway remodeling of COPD patients. Methods: The SPF male Wistar rats were assigned to the normal group, the model group, low, moderate and high doses groups of YuPingFeng powder joined with SiWu decoction using random number table method, ten rats each group. COPD rat model was established using intratracheal instillation of lipopolysaccharide and fumigation. The contents of SIgA, SC, TGF-β_1, and VEGF were measured after collecting lung tissue homogenate of the rats. Results: Compared with the normal group, the contents of SIgA, SC, TGF-β_1, and VEGF rose notably in the model group, and the difference showed statistical meaning(P< 0.05); Compared with the model group, the contents of SIgA, SC, TGF-β_1 and VEGF decreased remarkably in low dose group of YuPingFeng powder joined with SiWu decoction,and the difference was statistically significant(P < 0.01); moderate dose group showed slight effects on TGF-β_1, better effects on other three indexes(P< 0.05, P< 0.01), high dose group only had effects on VEGF(P < 0.01). Conclusion: Y uPingFeng powder joined with SiWu decoction could effectively reduce the contents of SIgA, SC, TGF-β_1, and VEGF in COPD rats, which might be one of the important mechanisms of treating COPD.
Objective:To investigate the effects of YuPingFeng powder joined with SiWu decoction on airway remodeling of COPD patients. Methods: The SPF male Wistar rats were assigned to the normal group, the model group, low, moderate and high doses groups of YuPingFeng powder joined with SiWu decoction using random number table method, ten rats each group. COPD rat model was established using intratracheal instillation of lipopolysaccharide and fumigation. The contents of SIgA, SC, TGF-β_1, and VEGF were measured after collecting lung tissue homogenate of the rats. Results: Compared with the normal group, the contents of SIgA, SC, TGF-β_1, and VEGF rose notably in the model group, and the difference showed statistical meaning(P< 0.05); Compared with the model group, the contents of SIgA, SC, TGF-β_1 and VEGF decreased remarkably in low dose group of YuPingFeng powder joined with SiWu decoction,and the difference was statistically significant(P < 0.01); moderate dose group showed slight effects on TGF-β_1, better effects on other three indexes(P< 0.05, P< 0.01), high dose group only had effects on VEGF(P < 0.01). Conclusion: Y uPingFeng powder joined with SiWu decoction could effectively reduce the contents of SIgA, SC, TGF-β_1, and VEGF in COPD rats, which might be one of the important mechanisms of treating COPD.
引文
[1]朱晓奕,洪庆,徐卫东,等.玉屏风散及原药材免疫调节活性研究进展[J].亚太传统医药,2017,13(9):66-68.
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[11]黄少君,傅汝梅.培土生金方对慢性阻塞性肺疾病稳定期患者气道重塑机制的观察[J].中国实验方剂学杂志,2018,24(1):181-186.
[12]刘领,吴文杰,杨霁,等.TNF-α、TGF-β及IL-10在慢性阻塞性肺疾病患者中的表达及临床意义[J].中国当代医药,2016,23(9):60-62.
[13]吕倩,王昌明,蒋明,等.HIF-1α和VEGF在大鼠COPD中的表达及与肺血管重构的关系研究[J].中国药理学通报,2012,28(6):772-777.
[14]向本旭,刘婷婷,孙芳玲,等.VEGF相关信号通路在血管新生中的研究进展[J].中国比较医学杂志,2015,25(12):81-86.
[2]康云峰.玉屏风散防治反复上呼吸道感染作用机制研究[J].临床医学研究与实践,2017,24(1):126-127.
[3]胡琦,郭平.四物汤补血机制研究进展[J].山东中医杂志,2017,36(9):819-821.
[4]何然,汪宏锦,周莹,等.四物汤对幼鼠缺铁性贫血的改善及其对铁代谢的调节作用[J].中国中药杂志,2017,42(5):944-950.
[5]陈平.慢性阻塞性肺疾病气道重塑的中医病机分析及防治探讨[J].辽宁中医杂志,2009,36(8):1296-1297.
[6]张伟,韩佳.慢性阻塞性肺疾病从气论治的病因病机探讨[J].中华中医药学刊,2012,10(30):2151-2153.
[7]何海滨,张艳丽,陈孝银.慢性阻塞性肺疾病稳定期从气论治[J].时珍国医国药,2008,19(2):475-476.
[8]姜腾,刘冬妍.呼吸道分泌型Ig A研究进展[J].中国免疫学杂志,2011,27(11):1041-1043.
[9]张宝中,冉多良,童贻刚.分泌型免疫球蛋白A的研究进展[J].生物技术通讯,2009,20(2):263-265.
[10]付金龙,刘沛.SC结构、功能及其临床研究进展[J].世界华人消化杂志,2009,17(3):293-298.
[11]黄少君,傅汝梅.培土生金方对慢性阻塞性肺疾病稳定期患者气道重塑机制的观察[J].中国实验方剂学杂志,2018,24(1):181-186.
[12]刘领,吴文杰,杨霁,等.TNF-α、TGF-β及IL-10在慢性阻塞性肺疾病患者中的表达及临床意义[J].中国当代医药,2016,23(9):60-62.
[13]吕倩,王昌明,蒋明,等.HIF-1α和VEGF在大鼠COPD中的表达及与肺血管重构的关系研究[J].中国药理学通报,2012,28(6):772-777.
[14]向本旭,刘婷婷,孙芳玲,等.VEGF相关信号通路在血管新生中的研究进展[J].中国比较医学杂志,2015,25(12):81-86.