不同CaCl_2浓度对低温胁迫下豇豆幼苗生理指标的影响
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摘要
为研究CaCl_2对蔬菜低温胁迫的缓解效应,以豇豆幼苗为试验材料,在10℃低温处理条件下用不同浓度(0、5、10、20、40 mmol·L~(-1))CaCl_2喷施豇豆幼苗叶片。结果表明,喷施CaCl_2能普遍提高豇豆幼苗生物量、相对含水量、光合色素含量、脯氨酸含量、可溶性糖含量及抗氧化酶活性,降低其相对电导率及丙二醛含量。且10 mmol·L~(-1)CaCl_2处理后,豇豆幼苗各部分生物量、光合色素含量达到最大,超氧化物岐化酶活性较对照增加99.6%、过氧化氢酶活性较对照增加29.49%,且相对电导率、丙二醛含量分别较对照降低62.07%、36.15%。因此,CaCl_2能够缓解低温胁迫对豇豆生理生态的影响,且浓度为10 mmol·L~(-1)的CaCl_2效果最好。
In order to study the alleviation effect of different concentrations of CaCl_2 on physiological characteristics of cowpea seedlings under low temperature.The leaves of cowpea seedlings were foliage sprayed with different concentrations(0,5,10,20,40 mmol·L~(-1))of CaCl_2 under low temperature treatment(10℃).The results showed that the biomass,relative water content,photosynthetic pigment content,proline content,soluble sugar content and the activities of antioxidant enzymes of cowpea seedlings were all increased after foliage spraying with CaCl_2,the relative conductivity and malondialdehyde content of cowpea seedlings were decreased.The biomass and content of photosynthetic pigment reached the maximum under 10 mmol·L~(-1)CaCl_2 concentration treatment,compared with the control,the activity of superoxide dismutase increased 99.6%,the catalase activity increased 29.49%,the relative conductivity and malondialdehyde content decreased 62.07%,36.15% respectively.In conclusion,10 mmol·L~(-1)CaCl_2 showed the best alleviation effect on the cowpea seedlings under low temperature.
In order to study the alleviation effect of different concentrations of CaCl_2 on physiological characteristics of cowpea seedlings under low temperature.The leaves of cowpea seedlings were foliage sprayed with different concentrations(0,5,10,20,40 mmol·L~(-1))of CaCl_2 under low temperature treatment(10℃).The results showed that the biomass,relative water content,photosynthetic pigment content,proline content,soluble sugar content and the activities of antioxidant enzymes of cowpea seedlings were all increased after foliage spraying with CaCl_2,the relative conductivity and malondialdehyde content of cowpea seedlings were decreased.The biomass and content of photosynthetic pigment reached the maximum under 10 mmol·L~(-1)CaCl_2 concentration treatment,compared with the control,the activity of superoxide dismutase increased 99.6%,the catalase activity increased 29.49%,the relative conductivity and malondialdehyde content decreased 62.07%,36.15% respectively.In conclusion,10 mmol·L~(-1)CaCl_2 showed the best alleviation effect on the cowpea seedlings under low temperature.
引文
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[21]周茹宝,张有福,张满效,等.两种圆柏属植物叶片代谢产物季节变化与抗冻性的关系[J].冰川冻土,2008,30(2):351-353.
[22]吴雪霞,查丁石,邰翔.低温胁迫对茄子幼苗生长、抗氧化酶活性和渗透调节物质的影响[J].江苏农业学报,2008,24(4):471-475.
[23]徐传保,戴庆敏.低温胁迫对竹子3种渗透调节物质的影响[J].河南农业科学,2011,40(1):127-130.
[24]高媛,齐晓花,杨景华,等.高等植物对低温胁迫的响应研究[J].北方园艺,2007(10):58-61.
[25]张继澍.植物生理学[M].西安:世界图书出版公司,1999:370-376.
[26]李春燕,陈思思,徐雯.苗期低温胁迫对扬麦16叶片抗氧化酶和渗透调节物质的影响[J].作物学报,2011,37(12):2293-2298.
[27]ALSCHER RG,ERTURK N.Role of superoxide dismutases(SODs)in controlling oxidative stress in plants[J].Journal of Experimental Botany,2002,53(372):1331-1341.
[28]尚相莲.蔬菜低温胁迫与抗冷性研究进展[J].长江蔬菜,2002(S1):18-20.
[2]艾琳,张萍,胡成志.低温胁迫对葡萄根系膜系统和可溶性糖及脯氨酸含量的影响[J].新疆农业大学学报,2004,27(4):47-50.
[3]龚鹏,李秀霞,李增萍,等.低温胁迫对扁桃枝条细胞膜系统和渗透调节物质的影响[J].新疆农业科学,2007,44(5):561-566.
[4]王华,王飞,陈登文,等.低温胁迫对杏花SOD活性和膜脂过氧化的影响[J].果树科学,2000,17(3):197-201.
[5]邵怡若,许建新,薛立,等.低温胁迫时间对4种幼苗生理生化及光合特性的影响[J].生态学报,2013,33(14):4237-4247.
[6]刘荣梅,胡国富,魏琪,等.低温对转CBF3基因烟草叶绿体超微结构和生理特性的影响[J].安徽农业科学,2010,38(18):9501-9503.
[7]王春利.低温胁迫下喷施CaCl2对油菜抗寒性的影响[J].湖北农业科学,2001(5):29-30.
[8]王小媚,任惠,刘业强,等.不同浓度氯化钙喷施番木瓜幼苗低温胁迫下的生理响应[J].中国南方果树,2015,44(4):45-47.
[9]张琳,王甲辰,左强,等.外源钙对低温胁迫下番茄幼苗生化指标及膜伤害的研究[J].北方园艺,2011(12):24-26.
[10]唐宽强,刘守伟,吴凤芝.等.外源喷施CaCl2对低温逆境下番茄抗冷性及开花结果的影响[J].北方园艺,2013(11):10-14.
[11]康国章,王正询,孙谷畴.几种外源物质提高植物抗冷性的生理机制[J].植物生理学通讯,2002,38(2):193-197.
[12]谢彦超,蒋生发.豇豆秋延后优质丰产栽培技术[J].南方园艺,2009,20(2):48-50.
[13]于锡宏,蒋欣梅,刁艳,等.脱落酸、水杨酸和氯化钙对番茄幼苗抗冷性的影响[J].东北农业大学学报,2010,41(5):42-45.
[14]廉洁,谷建田,张喜春.氯化钙对低温胁迫下番茄幼苗的影响[J].中国农学通报,2015,31(16):47-51.
[15]张永吉,李爱民,徐中友,等.不同浓度水杨酸对西瓜幼苗抗冷性的诱导效应[J].江苏农业科学,2016,44(3):183-185.
[16]罗芳芳,周睿,苏文华,等.干旱对滇中高原典型植物含水量的影响[J].安徽农业科学,2012,40(26):12745-12747.
[17]张以顺,黄霞,陈云凤.植物生理学实验教程[M].北京:高等教育出版社,2009.
[18]王学奎,黄见良.植物生理生化实验原理与技术[M].北京:高等教育出版社,2015.
[19]熊庆娥.植物生理学实验教程[M].成都:四川科学技术出版社,2003.
[20]MOKANY K,RAISON R J,PROKUSHKIN A S.Critical analysis of root:shoot ratios in terrestrial biomes[J].Global Change Biology,2006,12:84-96.
[21]周茹宝,张有福,张满效,等.两种圆柏属植物叶片代谢产物季节变化与抗冻性的关系[J].冰川冻土,2008,30(2):351-353.
[22]吴雪霞,查丁石,邰翔.低温胁迫对茄子幼苗生长、抗氧化酶活性和渗透调节物质的影响[J].江苏农业学报,2008,24(4):471-475.
[23]徐传保,戴庆敏.低温胁迫对竹子3种渗透调节物质的影响[J].河南农业科学,2011,40(1):127-130.
[24]高媛,齐晓花,杨景华,等.高等植物对低温胁迫的响应研究[J].北方园艺,2007(10):58-61.
[25]张继澍.植物生理学[M].西安:世界图书出版公司,1999:370-376.
[26]李春燕,陈思思,徐雯.苗期低温胁迫对扬麦16叶片抗氧化酶和渗透调节物质的影响[J].作物学报,2011,37(12):2293-2298.
[27]ALSCHER RG,ERTURK N.Role of superoxide dismutases(SODs)in controlling oxidative stress in plants[J].Journal of Experimental Botany,2002,53(372):1331-1341.
[28]尚相莲.蔬菜低温胁迫与抗冷性研究进展[J].长江蔬菜,2002(S1):18-20.