甘遂入药对比呋塞米对带瘤癌性腹水大鼠AQP-1表达及心肌损伤影响研究
|
摘要
目的:分析甘遂入药对比呋塞米对癌性腹水大鼠心肌受损与水通道蛋白-1(AQP-1)表达状况影响。方法:选取SPF级Wistar雄性大鼠60只,随机数字表法分成四组,正常组、模型组、西药组和观察组,每组各15只,除正常组外其它各组制备癌性腹水大鼠模型;造模以后第四天开始灌胃,西药组灌胃呋塞米药液,剂量为1ml/100g,观察组大鼠灌胃甘遂半夏汤和呋塞米药液,剂量为1ml/100g,模型组、正常组灌胃同剂量蒸馏水,各组大鼠均连续灌胃10天。记录大鼠体重、腹围/体重、尿量及腹水量,ELISA法检测血清醛固酮、钠离子、钾离子、Ure、血清肌酐(Cre)、尿酸(UA)、肌酸激酶(CK)、乳酸脱氢酶(LDH)、丙二醛(MDA)及超氧化物歧化酶(SOD)含量,同时检测大鼠腹膜组织AQP-1mRNA表达状况。结果:模型组、西药组及观察组大鼠体重较正常组下降,腹围/体重较正常上升,差异均有统计学意义(P<0.05);模型组、西药组大鼠醛固酮、钾离子及钠离子含量比正常组上升,AQP-1mRNA表达量比正常组下降,观察组醛固酮、钾离子及钠离子含量比模型组下降,AQP-1mRNA表达量比模型组上升,差异均有统计学意义(P<0.05);模型组、西药组及观察组大鼠Cre含量比正常组上升,Ck含量比正常组下降,模型组与西药组大鼠Ure、UA、LDH比正常组上升,观察组比模型组下降差异均有统计学意义(P<0.05),各组大鼠MDA、SOD差异均无统计学意义(P>0.05)。结论:甘遂半夏汤结合呋塞米可提升癌性腹水大鼠腹膜组织内AQP-1表达量,改善心肌受损状况。
Objective:To analyze the effect of Gansui Banxia Decoction on the expression of myocardial damage and aquaporin-1(AQP-1) for rats with cancerous ascites.Methods:Sixty Wistar male rats of SPF grade were randomly divided into four groups:normal group,model group,western medicine group and observation group,with 15 rats in each group.The other groups except the normal group prepared cancerous ascites.The fourth day after modeling,the stomach was administered,and the western medicine group was given the furosemide solution at a dose of 1 ml/100 g.The rats in the observation group were intragastrically administered with Ganzi Banxia Decoction and furosemide solution at a dose of 1 ml/100 g,the model group and the normal group were given the same amount of distilled water,and the rats in each group were continuously intragastrically administered for 10 days.Rat body weight,abdominal circumference/body weight,urine volume and ascites volume were recorded.Serum aldosterone,sodium ion,potassium ion,Ure,serum creatinine(Cre),uric acid(UA),creatine kinase(Ck),lactate were detected by ELISA.The contents of hydrogenase(LDH),malondialdehyde(MDA) and superoxide dismutase(SOD) were detected simultaneously,and the expression of AQP-1 mRNA in rat peritoneal tissues was detected.Results:The weight of the rats in the model group,the western medicine group and the observation group decreased compared with the normal group,and the abdominal circumference/body weight increased compared with normal(P<0.05).The aldosterone and potassium ions in the model group and western medicine group.The content of sodium ion was higher than that of the normal group.The expression of AQP-1 mRNA was lower than that of the normal group.The levels of aldosterone,potassium and sodium in the observation group were lower than those in the model group,and the expression of AQP-1 mRNA was higher than that in the model group.The difference was statistically significant(P<0.05);The content of Cre in the model group,western medicine group and observation group increased compared with the normal group,and the Ck content decreased compared with the normal group.The Ure,UA and LDH of the model group and the western medicine group were higher than the normal group,and the observation group was compared with the model group.The difference was statistically significant(P<0.05).There was no significant difference in MDA and SOD between the groups(P>0.05).Conclusion:Ganzi Banxia Decoction combined with furosemide can increase the expression of AQP-1 in peritoneal tissues of rats with cancerous ascites and improve myocardial damage.
Objective:To analyze the effect of Gansui Banxia Decoction on the expression of myocardial damage and aquaporin-1(AQP-1) for rats with cancerous ascites.Methods:Sixty Wistar male rats of SPF grade were randomly divided into four groups:normal group,model group,western medicine group and observation group,with 15 rats in each group.The other groups except the normal group prepared cancerous ascites.The fourth day after modeling,the stomach was administered,and the western medicine group was given the furosemide solution at a dose of 1 ml/100 g.The rats in the observation group were intragastrically administered with Ganzi Banxia Decoction and furosemide solution at a dose of 1 ml/100 g,the model group and the normal group were given the same amount of distilled water,and the rats in each group were continuously intragastrically administered for 10 days.Rat body weight,abdominal circumference/body weight,urine volume and ascites volume were recorded.Serum aldosterone,sodium ion,potassium ion,Ure,serum creatinine(Cre),uric acid(UA),creatine kinase(Ck),lactate were detected by ELISA.The contents of hydrogenase(LDH),malondialdehyde(MDA) and superoxide dismutase(SOD) were detected simultaneously,and the expression of AQP-1 mRNA in rat peritoneal tissues was detected.Results:The weight of the rats in the model group,the western medicine group and the observation group decreased compared with the normal group,and the abdominal circumference/body weight increased compared with normal(P<0.05).The aldosterone and potassium ions in the model group and western medicine group.The content of sodium ion was higher than that of the normal group.The expression of AQP-1 mRNA was lower than that of the normal group.The levels of aldosterone,potassium and sodium in the observation group were lower than those in the model group,and the expression of AQP-1 mRNA was higher than that in the model group.The difference was statistically significant(P<0.05);The content of Cre in the model group,western medicine group and observation group increased compared with the normal group,and the Ck content decreased compared with the normal group.The Ure,UA and LDH of the model group and the western medicine group were higher than the normal group,and the observation group was compared with the model group.The difference was statistically significant(P<0.05).There was no significant difference in MDA and SOD between the groups(P>0.05).Conclusion:Ganzi Banxia Decoction combined with furosemide can increase the expression of AQP-1 in peritoneal tissues of rats with cancerous ascites and improve myocardial damage.
引文
[1] 姚芳,曹雨诞,张楷承,等.癌性腹水模型制备方法及在中医药作用机制研究中的应用[J].中国药房,2018,7(6):203～208
[2] 张桥,曹亮亮,楼坚伟,等.甘遂醋制前后对癌性腹水模型大鼠泻水逐饮功效比较[J].中草药,2016,47(14):2492～2496
[3] Emoto S,Kurano M,Kano K,et al.Analysis of glycero-lysophospholipids in gastric cancerous ascites[J].Journal of Lipid Research,2017,58(4):763～766
[4] 赵健明,吴博.参芪抑瘤方联合腹腔热化疗对胃癌性腹水大鼠一般状态的观察[J].解放军医药杂志,2017,29(5):21～24
[5] 曾元儿.中国药典2010年版(一部) 化学成分分析简明手册[M].广州:中山大学出版社,2010:142～146
[6] 张建美,许皖,钟赣生,等.甘遂半夏汤加减甘遂甘草不同给药次数对腹水大鼠水通道蛋白-2基因及肾凋亡的影响[J].环球中医药,2016,9(5):539～543
[7] Seydi E,Salimi A,Rasekh H R,et al.Selective Cytotoxicity of Luteolin and Kaempferol on Cancerous Hepatocytes Obtained from Rat Model of Hepatocellular Carcinoma:Involvement of ROS-Mediated Mitochondrial Targeting.[J].Microbial Pathogenesis,2018,19(4):1～9
[8] 蔡亚红,洪佳娜.中药脐疗联合盐包热熨治疗癌性腹水20例[J].中国针灸,2016,36(5):497～498
[9] Teke K,Guzel N,Uslubas A K,et al.Monitoring the response of urothelial precancerous lesions to Bacillus Calmette-Guerin at the proteome level in an in vivo rat model[J].Cancer Immunology Immunotherapy,2016,15(10):1～11
[10] 许皖,柳海艳,钟赣生,等.甘遂半夏汤加减甘遂及不同品种甘草对腹水模型大鼠肝脏CYP450mRNA的影响[J].环球中医药,2016,9(5):521～527
[11] 张虹,徐悦涛,邹巧玲,等.华蟾素注射液联合顺铂腔内灌注治疗癌性胸腹水临床研究[J].中医学报,2016,31(8):1096～1098
[12] 聂卢赛娜,郑亮.从“血不利则为水”论治癌性腹水[J].辽宁中医药大学学报,2017,9(12):177～180
[13] 赵若琳,殷佩浩,倪振华,等.芝类提取物改善Walker-256腹水大鼠免疫功能[J].第二军医大学学报,2017,38(8):1016～1022
[14] Kawada J,Nishino M,Hata T,et al.A Case of Effective Palliative Care with CART for Refractory Ascites Associated with Cancerous Peritonitis of Gastric Cancer[J].Gan to Kagaku Ryoho,2018,45(4):700～702
[15] 顾芳红,周建伟,张翔.行气利水散敷脐治疗癌性腹水的临床观察[J].上海中医药杂志,2016,8(5):39～41
[16] 宋凤丽,朱旭,高辰,等.温阳消水方治疗癌性腹水的临床观察及机制研究[J].中华中医药杂志,2016,4(4):1513～1516
[2] 张桥,曹亮亮,楼坚伟,等.甘遂醋制前后对癌性腹水模型大鼠泻水逐饮功效比较[J].中草药,2016,47(14):2492～2496
[3] Emoto S,Kurano M,Kano K,et al.Analysis of glycero-lysophospholipids in gastric cancerous ascites[J].Journal of Lipid Research,2017,58(4):763～766
[4] 赵健明,吴博.参芪抑瘤方联合腹腔热化疗对胃癌性腹水大鼠一般状态的观察[J].解放军医药杂志,2017,29(5):21～24
[5] 曾元儿.中国药典2010年版(一部) 化学成分分析简明手册[M].广州:中山大学出版社,2010:142～146
[6] 张建美,许皖,钟赣生,等.甘遂半夏汤加减甘遂甘草不同给药次数对腹水大鼠水通道蛋白-2基因及肾凋亡的影响[J].环球中医药,2016,9(5):539～543
[7] Seydi E,Salimi A,Rasekh H R,et al.Selective Cytotoxicity of Luteolin and Kaempferol on Cancerous Hepatocytes Obtained from Rat Model of Hepatocellular Carcinoma:Involvement of ROS-Mediated Mitochondrial Targeting.[J].Microbial Pathogenesis,2018,19(4):1～9
[8] 蔡亚红,洪佳娜.中药脐疗联合盐包热熨治疗癌性腹水20例[J].中国针灸,2016,36(5):497～498
[9] Teke K,Guzel N,Uslubas A K,et al.Monitoring the response of urothelial precancerous lesions to Bacillus Calmette-Guerin at the proteome level in an in vivo rat model[J].Cancer Immunology Immunotherapy,2016,15(10):1～11
[10] 许皖,柳海艳,钟赣生,等.甘遂半夏汤加减甘遂及不同品种甘草对腹水模型大鼠肝脏CYP450mRNA的影响[J].环球中医药,2016,9(5):521～527
[11] 张虹,徐悦涛,邹巧玲,等.华蟾素注射液联合顺铂腔内灌注治疗癌性胸腹水临床研究[J].中医学报,2016,31(8):1096～1098
[12] 聂卢赛娜,郑亮.从“血不利则为水”论治癌性腹水[J].辽宁中医药大学学报,2017,9(12):177～180
[13] 赵若琳,殷佩浩,倪振华,等.芝类提取物改善Walker-256腹水大鼠免疫功能[J].第二军医大学学报,2017,38(8):1016～1022
[14] Kawada J,Nishino M,Hata T,et al.A Case of Effective Palliative Care with CART for Refractory Ascites Associated with Cancerous Peritonitis of Gastric Cancer[J].Gan to Kagaku Ryoho,2018,45(4):700～702
[15] 顾芳红,周建伟,张翔.行气利水散敷脐治疗癌性腹水的临床观察[J].上海中医药杂志,2016,8(5):39～41
[16] 宋凤丽,朱旭,高辰,等.温阳消水方治疗癌性腹水的临床观察及机制研究[J].中华中医药杂志,2016,4(4):1513～1516