不同钙离子浓度对低温下降香黄檀幼苗生长及生理特性的影响
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摘要
以降香黄檀(Dalbergia odorifera T. Chen)为材料,通过人工控制温度的方法,探讨在不同温度条件下施加不同浓度Ca~(2+)对降香黄檀幼苗生长及生理特性的影响。结果显示:常温条件下,施加不同浓度的Ca~(2+)对降香黄檀的茎高、叶长、净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)和光合色素含量均有显著影响;而对叶片相对含水量、露点水势、相对电导率、丙二醛(MDA)和可溶性蛋白含量影响较小;促进生长发育的最适Ca~(2+)浓度为5 mmol/L。低温对降香黄檀上述生理指标以及水分利用效率(WUE)均有显著影响;低温条件下施加不同浓度的Ca~(2+)能使上述指标得到不同程度的缓解,提高降香黄檀低温胁迫耐受性的最适浓度为5 mmol/L。隶属函数分析结果表明,常温和低温条件下,外源Ca~(2+)浓度对降香黄檀的调控影响依次为5、10、15、2.5 mmol/L。
Dalbergia odorifera T. Chen was used to study the effects of different concentrations of exogenous Ca~(2+) on seedling growth and physiological characteristics under different artificial temperature conditions. Significant effects were found on stem height,leaf length,Pn,Gs,Tr,and photosynthetic pigment content under different concentrations of Ca~(2+) at normal temperature. However,limited effects were found on the relative water content and dew point water potential of leaves, relative conductivity, MDA, and soluble protein content. The optimum Ca~(2+) concentration to improve plant growth was 5 mmol/L. In addition,stem height,leaf length,Pn,Gs,Tr,WUE,content of photosynthetic pigment,relative water content,dew point water potential,relative conductivity,MDA,and soluble protein content were significantly affected by cold stress. These negative effects could be alleviated by applying exogenous Ca~(2+) ,with the optimal Ca~(2+) concentration again found to be 5 mmol/L,followed by 10,15,and 2.5 mmol/L under normal temperature and cold stress. The results of this study provide scientific guidance in regard to the adaptation of D. odorifera to cold stress and its cultivation in low temperature regions of subtropical areas.
Dalbergia odorifera T. Chen was used to study the effects of different concentrations of exogenous Ca~(2+) on seedling growth and physiological characteristics under different artificial temperature conditions. Significant effects were found on stem height,leaf length,Pn,Gs,Tr,and photosynthetic pigment content under different concentrations of Ca~(2+) at normal temperature. However,limited effects were found on the relative water content and dew point water potential of leaves, relative conductivity, MDA, and soluble protein content. The optimum Ca~(2+) concentration to improve plant growth was 5 mmol/L. In addition,stem height,leaf length,Pn,Gs,Tr,WUE,content of photosynthetic pigment,relative water content,dew point water potential,relative conductivity,MDA,and soluble protein content were significantly affected by cold stress. These negative effects could be alleviated by applying exogenous Ca~(2+) ,with the optimal Ca~(2+) concentration again found to be 5 mmol/L,followed by 10,15,and 2.5 mmol/L under normal temperature and cold stress. The results of this study provide scientific guidance in regard to the adaptation of D. odorifera to cold stress and its cultivation in low temperature regions of subtropical areas.
引文
[1]赵霞,徐大平,杨曾奖,刘小金,张宁南.养分胁迫对降香黄檀幼苗生长及叶片养分状况的影响[J].生态学杂志,2017,36(6):1503-1508.Zhao X,Xu DP,Yang ZJ,Liu XJ,Zhang NN.Effects of nutrient stress on seedling growth and foliar nutrient status of Dalbergia odorifera[J].Chinese Journal of Ecology,2017,36(6):1503-1508.
[2]梁建平,蒋军林,秦武明,吴道念,严理,等.广西南宁46年生降香黄檀人工林生长规律[J].浙江农林大学学报,2015,32(4):523-528.Liang JP,Jiang JL,Qin WM,Wu DN,Yan L,et al.Growth rule for a 46-year-old Dalbergia odorifera in Nanning,Guangxi[J].Journal of Zhejiang Forestry College,2015,32(4):523-528.
[3]连辉明,张谦,殷祚云,蔡燕灵,曾令海,等.广东降香黄檀人工林生长及寒害调查分析[J].中南林业科技大学学报,2014,34(10):26-31.Lian HM,Zhang Q,Yin ZY,Cai YL,Zeng LH,et al.Survey and analysis on growth and chilling injury of Dalbergia odorifera plantation in Guangdong province[J].Journal of Central South Forestry University,2014,34(10):26-31.
[4]叶水西.降香黄檀扦插育苗技术初步研究[J].安徽农学通报,2008,14(9):128-129.Ye SX.Preliminary study on cutting of Dalbergia odorifera[J].Auhui Agricultural Science Bulletin,2008,14(9):128-129.
[5]倪臻,王凌晖,吴国欣,刘运华,秦武明,等.降香黄檀引种栽培技术研究概述[J].福建林业科技,2008,35(2):265-268.Ni Z,Wang LH,Wu GX,Liu YH,Qin WM,et al.A review of studies on the introduction and cultivation-technology of Dalbergia odorifera[J].Journal of Fujian Forestry Science and Technology,2008,35(2):265-268.
[6]黄建红.川南地区引种降香黄檀造林试验初报[J].四川林业科技,2018,39(2):37-39,107.Huang JH.A preliminary report on afforestation with Dalbergia odorifera in Yibin[J].Journal of Sichuan Forestry Science and Technology,2018,39(2):37-39,107.
[7]Zhang S,Jiang H,Peng S,Korpelainen H,Li C.Sexrelated differences in morphological,physiological,and ultrastructural responses of Populus cathayana to chilling[J].J Exp Bot,2011,62:675-686.
[8]Huseynova IM.Photosynthetic characteristics and enzymatic antioxidant capacity of leaves from wheat cultivars exposed to drought[J].BBA-Bioenergetics,2012,1817(8):1516-1523.
[9]Yang F,Miao LF.Adaptive responses to progressive drought stress in two poplar species originating from different altitudes[J].Silva Fenn,2010,44(1):23-37.
[10]Yang F,Wang Y,Miao LF.Comparative physiological and proteomic responses to drought stress in two poplar species originating from different altitudes[J].Physiol plantarum,2010,139(4):388-400.
[11]Chinnusamy V,Zhu JK,Sunkar R.Gene regulation during cold stress acclimation in plants[J].Methods Mol Biol,2010,639(1):39-55.
[12]Cramer GR.Sodium-calcium interactions under salinity stress[M]//Luchli A,Lüttge U,eds.Salinity:Environment-Plants-Molecules.New York:Kluwer Academic Publishers,2002.
[13]Srivastava AK,Rai AN,Patade VY,Suprasanna P.Calcium signaling and its significance in alleviating salt stress in plants[M]//Ahmad P,Azooz MM,Prasad MNV,eds.Salt Stress in Plants:Signalling,Omics and Adaptations.New York:Springer,2013.
[14]Monroy AF,Sarhan F,Dhindsa RS.Cold-induced changes in freezing tolerance,protein phosphorylation,and gene expression(evidence for a role of calcium)[J].Plant Physiol,1993,102(4):1227-1235.
[15]Shi YX,Guan AM,Li BJ.Accumulation and activity of phytoalexins in cucumber induced by pathogenicityimparired strain of Cladosporium cucumernium[J].Acta Horticulturae Sinica,2007,34(2):361-365.
[16]Mahajan S,Pandey GK,Tuteja N.Calcium-and saltstress signaling in plants:shedding light on SOS pathway[J].Arch Biochem Biophys,2008,471(2):146-158.
[17]Gao HB,Chen GL.Effect of calcium on chilling resistance of grafted eggplant seedlings[J].J Plant Nutr,2004,27(8):1327-1339.
[18]廖金柯,赵克,胡小燕,杨龙,马媛,朱新霞.外源钙对棉花幼苗抗冷性的影响[J].新疆农业科学,2012,49(4):687-693.Liao JK,Zhao K,Hu XY,Yang L,Ma Y,Zhu XX.Influence of exogenous Ca2+on chilling resistance of cotton seedlings[J].Xinjiang Agricultural Sciences,2012,49(4):687-693.
[19]闫秋洁,黄丹,辜英琼,李旭锋,杨毅.外源钙对自然低温下富贵竹叶片生长的影响[J].北方园艺,2015(3):66-69.Yan QJ,Huang D,Gu YQ,Li XF,Yang Y.Comparative analysis of drought-resistant ability of three ornamental bamboo[J].Northern Horticulture,2015(3):66-69.
[20]赵娟,王芳,李永生,姚海梅,张同祯,等.钙对低温胁迫下玉米种子萌发及幼苗生长的影响[J].甘肃农业大学学报,2016,51(6):30-35.Zhao J,Wang F,Li YS,Yao HM,Zhang TZ,et al.Effects of calcium on maize seed germination and seedling growth under low temperature stress[J].Journal of Gansu Agricultural University,2016,51(6):30-35.
[21]刘国花,兰建彬,刘奕清,朱菁,郑春花.外源钙对低温胁迫下尾巨桉幼苗生理特性及内源激素的影响[J].西北林学院学报,2017,32(6):101-106.Liu GH,Lan JB,Liu YQ,Zhu J,Zheng CH.Effects of exogenous calcium on seedling physiological characteristics and endogenous hormone of Eucalyptus urophylla×E.grandis under low temperature stress[J].Journal of Northwest forestry university,2017,32(6):101-106.
[22]刘玲.外源钙离子(Ca2+)对万寿菊幼苗抗冷性的影响[J].安徽农学通报,2008,14(18):113-113.Liu L.Effect of exogenous calcium ion(Ca2+)on chilling resistance of Tagetes erecta L.seedlings[J].Auhui Agricultural Science Bulletin,2008,14(18):113-113.
[23]Xu X,Yang F,Xiao XW,Zhang S,Korpelainen H,Li CY.Sex-specific responses of Populus cathayana to drought and elevated temperatures[J].Plant Cell Environ,2008,31(6):850-860.
[24]Dong JF,Yu Q,Lu L,Xu MJ.Effect of yeast saccharide treatment on nitric oxide accumulation and chilling injury in cucumber fruit during cold storage[J].Postharvest Biol Tec,2012,68:1-7.
[25]Yang F,Wang Y,Wang J,Deng WQ,Liao L,Li M.Different eco-physiological responses between male and female Populus deltoides clones to waterlogging stress[J].Forest Ecol Manag,2011,262(11):1963-1971.
[26]苗灵凤,张丽佳,蒲玉瑾,杨帆.WP4C露点水势仪测定植物叶片水势时的影响因素:以降香黄檀为例[J].植物科学学报,2017,35(1):93-98.Miao LF,Zhang LJ,Pu YJ,Yang F.Factors that affect measurement using the WP4C dewpoint potential meter to determine water potential:Illustrated by the case of Dalbergia odorifera[J].Plant Science Journal,2017,35(1):93-98.
[27]Han C,Chan Z,Yang F.Comparative analyses of universal extraction buffers for assay of stress related biochemical and physiological parameters[J].Prep Biochem Biotech,2015,45(7):684-695.
[28]李广鲁,王文果,陈志新,胡增辉,冷平生.钙对盐胁迫下冰叶日中花不同器官离子含量和根部K+、Na+吸收的影响[J].植物科学学报,2018,36(2):282-290.Li GL,Wang WG,Chen ZX,Hu ZH,Leng PS.Effect of calcium onion contents in different organs and absorption of K+and Na+in the root tips of Mesembryanthemum crystallinum L.under Na Cl stress[J].Plant Science Journal,2018,36(2):282-290.
[29]付晓凤,王莉姗,朱原,许倩,王凌晖,滕维超.不同施肥处理对海南风吹楠幼苗生长及生理特性影响[J].植物科学学报,2018,36(2):273-281.Fu XF,Wang LS,Zhu Y,Xu Q,Wang LH,Teng WC.Effects of different fertilization treatments on the growth and physiological characteristics of Horsfieldia hainanensis Merr.Seedlings[J].Plant Science Journal,2018,36(2):273-281.
[30]刘铁铮,王献革,索相敏,刘国胜.外源钙及钙离子抑制剂对杏扁雌蕊抗寒性的影响[J].吉林农业科学,2015,40(5):90-92,101.Liu TZ,Wang XG,Suo XM,Liu GS.Effects of exogenous calcium and calcium ion inhibitor on cold resistance of pistil of kernel apricot[J].Jilin Agricultural Science,2015,40(5):90-92,101.
[31]严蓓,孙锦,束胜,郭世荣.外源钙对Na Cl胁迫下黄瓜幼苗叶片光合特性及碳水化合物代谢的影响[J].南京农业大学学报,2014,37(1):31-36.Yan B,Sun J,Su S,Guo SR.Effects of exogenous calcium on photosynthetic characteristics and carbohydrate metabolism in leaves of cucumber(Cucumis sativus L.)seedlings under Na Cl stress[J].Journal of Nanjing Agricultural University,2014,37(1):31-36.
[32]车秀霞,陈惠萍,严巧娣,李永慧,杨蓓芬,等.外源钙离子与南方菟丝子寄生对喜旱莲子草茎形态结构的影响[J].生态学报,2013,33(9):2695-2702.Che XX,Chen HP,Yan QD,Li YH,Yang BF,et al.Antagonistic interactive effects of exogenous calcium ions and parasitic Cuscuta australis on the morphology and structure of Alternanthera philoxeroides stem[J].Acta Ecologica Sinica,2013,33(9):2695-2702.
[33]Yang BZ,Liu ZB,Zhou SD,Ou LJ,Dai XZ,et al.Exogenous Ca2+alleviates waterlogging caused damages to pepper[J].Photosynthetica,2016,54(4):1-11.
[2]梁建平,蒋军林,秦武明,吴道念,严理,等.广西南宁46年生降香黄檀人工林生长规律[J].浙江农林大学学报,2015,32(4):523-528.Liang JP,Jiang JL,Qin WM,Wu DN,Yan L,et al.Growth rule for a 46-year-old Dalbergia odorifera in Nanning,Guangxi[J].Journal of Zhejiang Forestry College,2015,32(4):523-528.
[3]连辉明,张谦,殷祚云,蔡燕灵,曾令海,等.广东降香黄檀人工林生长及寒害调查分析[J].中南林业科技大学学报,2014,34(10):26-31.Lian HM,Zhang Q,Yin ZY,Cai YL,Zeng LH,et al.Survey and analysis on growth and chilling injury of Dalbergia odorifera plantation in Guangdong province[J].Journal of Central South Forestry University,2014,34(10):26-31.
[4]叶水西.降香黄檀扦插育苗技术初步研究[J].安徽农学通报,2008,14(9):128-129.Ye SX.Preliminary study on cutting of Dalbergia odorifera[J].Auhui Agricultural Science Bulletin,2008,14(9):128-129.
[5]倪臻,王凌晖,吴国欣,刘运华,秦武明,等.降香黄檀引种栽培技术研究概述[J].福建林业科技,2008,35(2):265-268.Ni Z,Wang LH,Wu GX,Liu YH,Qin WM,et al.A review of studies on the introduction and cultivation-technology of Dalbergia odorifera[J].Journal of Fujian Forestry Science and Technology,2008,35(2):265-268.
[6]黄建红.川南地区引种降香黄檀造林试验初报[J].四川林业科技,2018,39(2):37-39,107.Huang JH.A preliminary report on afforestation with Dalbergia odorifera in Yibin[J].Journal of Sichuan Forestry Science and Technology,2018,39(2):37-39,107.
[7]Zhang S,Jiang H,Peng S,Korpelainen H,Li C.Sexrelated differences in morphological,physiological,and ultrastructural responses of Populus cathayana to chilling[J].J Exp Bot,2011,62:675-686.
[8]Huseynova IM.Photosynthetic characteristics and enzymatic antioxidant capacity of leaves from wheat cultivars exposed to drought[J].BBA-Bioenergetics,2012,1817(8):1516-1523.
[9]Yang F,Miao LF.Adaptive responses to progressive drought stress in two poplar species originating from different altitudes[J].Silva Fenn,2010,44(1):23-37.
[10]Yang F,Wang Y,Miao LF.Comparative physiological and proteomic responses to drought stress in two poplar species originating from different altitudes[J].Physiol plantarum,2010,139(4):388-400.
[11]Chinnusamy V,Zhu JK,Sunkar R.Gene regulation during cold stress acclimation in plants[J].Methods Mol Biol,2010,639(1):39-55.
[12]Cramer GR.Sodium-calcium interactions under salinity stress[M]//Luchli A,Lüttge U,eds.Salinity:Environment-Plants-Molecules.New York:Kluwer Academic Publishers,2002.
[13]Srivastava AK,Rai AN,Patade VY,Suprasanna P.Calcium signaling and its significance in alleviating salt stress in plants[M]//Ahmad P,Azooz MM,Prasad MNV,eds.Salt Stress in Plants:Signalling,Omics and Adaptations.New York:Springer,2013.
[14]Monroy AF,Sarhan F,Dhindsa RS.Cold-induced changes in freezing tolerance,protein phosphorylation,and gene expression(evidence for a role of calcium)[J].Plant Physiol,1993,102(4):1227-1235.
[15]Shi YX,Guan AM,Li BJ.Accumulation and activity of phytoalexins in cucumber induced by pathogenicityimparired strain of Cladosporium cucumernium[J].Acta Horticulturae Sinica,2007,34(2):361-365.
[16]Mahajan S,Pandey GK,Tuteja N.Calcium-and saltstress signaling in plants:shedding light on SOS pathway[J].Arch Biochem Biophys,2008,471(2):146-158.
[17]Gao HB,Chen GL.Effect of calcium on chilling resistance of grafted eggplant seedlings[J].J Plant Nutr,2004,27(8):1327-1339.
[18]廖金柯,赵克,胡小燕,杨龙,马媛,朱新霞.外源钙对棉花幼苗抗冷性的影响[J].新疆农业科学,2012,49(4):687-693.Liao JK,Zhao K,Hu XY,Yang L,Ma Y,Zhu XX.Influence of exogenous Ca2+on chilling resistance of cotton seedlings[J].Xinjiang Agricultural Sciences,2012,49(4):687-693.
[19]闫秋洁,黄丹,辜英琼,李旭锋,杨毅.外源钙对自然低温下富贵竹叶片生长的影响[J].北方园艺,2015(3):66-69.Yan QJ,Huang D,Gu YQ,Li XF,Yang Y.Comparative analysis of drought-resistant ability of three ornamental bamboo[J].Northern Horticulture,2015(3):66-69.
[20]赵娟,王芳,李永生,姚海梅,张同祯,等.钙对低温胁迫下玉米种子萌发及幼苗生长的影响[J].甘肃农业大学学报,2016,51(6):30-35.Zhao J,Wang F,Li YS,Yao HM,Zhang TZ,et al.Effects of calcium on maize seed germination and seedling growth under low temperature stress[J].Journal of Gansu Agricultural University,2016,51(6):30-35.
[21]刘国花,兰建彬,刘奕清,朱菁,郑春花.外源钙对低温胁迫下尾巨桉幼苗生理特性及内源激素的影响[J].西北林学院学报,2017,32(6):101-106.Liu GH,Lan JB,Liu YQ,Zhu J,Zheng CH.Effects of exogenous calcium on seedling physiological characteristics and endogenous hormone of Eucalyptus urophylla×E.grandis under low temperature stress[J].Journal of Northwest forestry university,2017,32(6):101-106.
[22]刘玲.外源钙离子(Ca2+)对万寿菊幼苗抗冷性的影响[J].安徽农学通报,2008,14(18):113-113.Liu L.Effect of exogenous calcium ion(Ca2+)on chilling resistance of Tagetes erecta L.seedlings[J].Auhui Agricultural Science Bulletin,2008,14(18):113-113.
[23]Xu X,Yang F,Xiao XW,Zhang S,Korpelainen H,Li CY.Sex-specific responses of Populus cathayana to drought and elevated temperatures[J].Plant Cell Environ,2008,31(6):850-860.
[24]Dong JF,Yu Q,Lu L,Xu MJ.Effect of yeast saccharide treatment on nitric oxide accumulation and chilling injury in cucumber fruit during cold storage[J].Postharvest Biol Tec,2012,68:1-7.
[25]Yang F,Wang Y,Wang J,Deng WQ,Liao L,Li M.Different eco-physiological responses between male and female Populus deltoides clones to waterlogging stress[J].Forest Ecol Manag,2011,262(11):1963-1971.
[26]苗灵凤,张丽佳,蒲玉瑾,杨帆.WP4C露点水势仪测定植物叶片水势时的影响因素:以降香黄檀为例[J].植物科学学报,2017,35(1):93-98.Miao LF,Zhang LJ,Pu YJ,Yang F.Factors that affect measurement using the WP4C dewpoint potential meter to determine water potential:Illustrated by the case of Dalbergia odorifera[J].Plant Science Journal,2017,35(1):93-98.
[27]Han C,Chan Z,Yang F.Comparative analyses of universal extraction buffers for assay of stress related biochemical and physiological parameters[J].Prep Biochem Biotech,2015,45(7):684-695.
[28]李广鲁,王文果,陈志新,胡增辉,冷平生.钙对盐胁迫下冰叶日中花不同器官离子含量和根部K+、Na+吸收的影响[J].植物科学学报,2018,36(2):282-290.Li GL,Wang WG,Chen ZX,Hu ZH,Leng PS.Effect of calcium onion contents in different organs and absorption of K+and Na+in the root tips of Mesembryanthemum crystallinum L.under Na Cl stress[J].Plant Science Journal,2018,36(2):282-290.
[29]付晓凤,王莉姗,朱原,许倩,王凌晖,滕维超.不同施肥处理对海南风吹楠幼苗生长及生理特性影响[J].植物科学学报,2018,36(2):273-281.Fu XF,Wang LS,Zhu Y,Xu Q,Wang LH,Teng WC.Effects of different fertilization treatments on the growth and physiological characteristics of Horsfieldia hainanensis Merr.Seedlings[J].Plant Science Journal,2018,36(2):273-281.
[30]刘铁铮,王献革,索相敏,刘国胜.外源钙及钙离子抑制剂对杏扁雌蕊抗寒性的影响[J].吉林农业科学,2015,40(5):90-92,101.Liu TZ,Wang XG,Suo XM,Liu GS.Effects of exogenous calcium and calcium ion inhibitor on cold resistance of pistil of kernel apricot[J].Jilin Agricultural Science,2015,40(5):90-92,101.
[31]严蓓,孙锦,束胜,郭世荣.外源钙对Na Cl胁迫下黄瓜幼苗叶片光合特性及碳水化合物代谢的影响[J].南京农业大学学报,2014,37(1):31-36.Yan B,Sun J,Su S,Guo SR.Effects of exogenous calcium on photosynthetic characteristics and carbohydrate metabolism in leaves of cucumber(Cucumis sativus L.)seedlings under Na Cl stress[J].Journal of Nanjing Agricultural University,2014,37(1):31-36.
[32]车秀霞,陈惠萍,严巧娣,李永慧,杨蓓芬,等.外源钙离子与南方菟丝子寄生对喜旱莲子草茎形态结构的影响[J].生态学报,2013,33(9):2695-2702.Che XX,Chen HP,Yan QD,Li YH,Yang BF,et al.Antagonistic interactive effects of exogenous calcium ions and parasitic Cuscuta australis on the morphology and structure of Alternanthera philoxeroides stem[J].Acta Ecologica Sinica,2013,33(9):2695-2702.
[33]Yang BZ,Liu ZB,Zhou SD,Ou LJ,Dai XZ,et al.Exogenous Ca2+alleviates waterlogging caused damages to pepper[J].Photosynthetica,2016,54(4):1-11.