苓泽合剂对急性痛风性关节炎大鼠血清IL-6、TNF-α含量的影响
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摘要
目的观察苓泽合剂对尿酸钠诱导的大鼠痛风性关节炎的抗炎作用,并探讨其作用机制。方法将80只Wistar大鼠随机分为空白组、模型组、芬必得组、苯溴马隆组、痛风定组及苓泽合剂低、中、高剂量组,每组10只。用尿酸钠诱导大鼠痛风性关节炎,测定血清中白细胞介素-6(interlenkin 6, IL-6)、肿瘤坏死因子-α(tumor necrosis factor-α, TNF-α)的含量。结果与模型组比较,各给药组血清中IL-6与TNF-α的含量均显著降低(P<0.01)。苓泽合剂中剂量组、高剂量组与低剂量组比较,血清中IL-6、TNF-α的含量明显下降(P<0.05)。结论苓泽合剂可抑制急性痛风性关节炎大鼠IL-6、TNF-α的表达,治疗痛风性关节炎。
Objective To explore the anti-inflammatory effect of Lingze Mixture on monosodium urate(MSU) crystal-induced gouty arthritis in rats and its mechanism of action. Methods Eighty Wistar rats were randomly divided into blank group, model group,fenbid group, benzbromarone group, Tongfengding group, and traditional Chinese medicine(TCM) Lingze Mixture groups(low, medium, and high doses), with ten rats in each group. A rat model of gouty arthritis was established by MSU crystals. Then the serum levels of interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) were determined. Results Compared with the model group, levels of IL-6 and TNF-α in serum in each model group were significantly decreased(P<0.01). Compared with the low-dose Lingze Mixture group, the medium-and high-dose Lingze Mixture groups had significantly reduced serum levels of IL-6 and TNF-α(P<0.05). Conclusion Lingze Mixture can inhibit the expression of IL-6 and TNF-α in rats with acute gouty arthritis, thus achieving the aim of preventing and alleviating gouty arthritis.
Objective To explore the anti-inflammatory effect of Lingze Mixture on monosodium urate(MSU) crystal-induced gouty arthritis in rats and its mechanism of action. Methods Eighty Wistar rats were randomly divided into blank group, model group,fenbid group, benzbromarone group, Tongfengding group, and traditional Chinese medicine(TCM) Lingze Mixture groups(low, medium, and high doses), with ten rats in each group. A rat model of gouty arthritis was established by MSU crystals. Then the serum levels of interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) were determined. Results Compared with the model group, levels of IL-6 and TNF-α in serum in each model group were significantly decreased(P<0.01). Compared with the low-dose Lingze Mixture group, the medium-and high-dose Lingze Mixture groups had significantly reduced serum levels of IL-6 and TNF-α(P<0.05). Conclusion Lingze Mixture can inhibit the expression of IL-6 and TNF-α in rats with acute gouty arthritis, thus achieving the aim of preventing and alleviating gouty arthritis.
引文
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[19]李振彬,马旭,杨静,等.白芍总苷治疗大鼠急性痛风性关节炎的作用及对血清IL-1β、IL-6、TNF-α的影响[J].新医学,2017,48(6):380-384.
[2] TONY M. Genomic Influences on Hyperuricemia and Gout[J].Rheumatic Disease Clinics of North America, 2017,43(3):389-399.
[3]陆小龙,曾朋,向黎黎,等.蠲痹历节清酊对大鼠急性痛风性关节炎模型滑膜组织中ICAM-1、NF-κB表达的影响[J].湖南中医药大学学报,2017,37(12):1299-1302.
[4]周彪,郭玉星,陆小龙,等.蠲痹历节清方对大鼠痛风性关节炎关节肿胀指数和滑膜组织中炎症因子的影响[J].云南中医学院学报,2017,40(3):15-18,32.
[5]张剑勇,王辉,谢静静,等.痛风泰颗粒对急性痛风大鼠IL-1、IL-6及TNF-α含量的影响[J].新中医,2016,48(12):201-203.
[6] DHANASEKAR C,RASOOL M. Morin, a dietary bioflavonol suppresses mon-osodium urate crystal-induced inflammation in an animal model of a-cute gouty arthritis with reference to NLRP3 inflammasome,hypo-xan-thine phosphor-ribosyl transferase,and inflammatory mediators[J]. Eur J Pharmacol,2016,7(86):116-127.
[7] FERNANDO M, GIEMBYCZ M, MC K. Bidirectional crosstalk via IL-6,PGE2 and PGD2 between murine myofibroblasts and alternative-ly activated macrophages enhances anti-inflammatory phenotype in both cells[J]. Br J Pharmacol,2016,173(5):899-912.
[8]刘昌盛,陈昉,何颖,等.白子菜提取物对急性痛风性关节炎大鼠模型抗炎作用的研究[J].湖南中医药大学学报,2016,36(8):26-29,81.
[9] GAO F, YANG Y, FENG X, et al. Interleukin-27 is elevated in sepsis-induced myocardial dysfunction and mediates inflammation[J]. Cyto-kine,2016,8(8):1-11.
[10]谢兴文,王春亮,徐世红,等.忍冬藤痛风颗粒对痛风性关节炎模型大鼠MMP-3和LP-PLA2的影响[J].中国中医骨伤科杂志,2016,24(2):6-8.
[11]侯玮婷,罗佳波.复方茯苓多糖口服液抗肿瘤作用和免疫调节功能的初步研究[J].中药药理与临床,2017,32(2):78-81.
[12]汪锦飘,刘永茂,何志超,等.泽泻乙醇提取物对氧嗪酸钾盐致大鼠高尿酸血症模型的影响[J].中成药,2017,39(3):605-608.
[13]林彬.黄柏生品与各种炮制品滋阴及抗痛风作用比较[J].医学理论与实践,2017,29(19):3304-3305.
[14]庞雪,刘玉强,才谦,等.苍术麸炒前后活性部位药效比较研究[J].中国药房,2016,27(10):1308-1311.
[15]司晓丽,王燕,党文呈,等.百合知母汤对支气管哮喘大鼠气道炎症的影响[J].中医杂志,2017,58(14):1499-1502.
[16]李琴.当归拈痛汤加减联合刺络放血法治疗急性痛风性关节炎临床观察[J].云南中医中药杂志,2017,38(3):18-19.
[17]张彦丽,张硕峰,华国栋,等.红花对华法林抗凝作用的影响[J].中医药信息,2016,33(1):32-35.
[18]姜卫星,王海燕,索成云,等.生姜醇提取物对肝脏缺血再灌注大鼠氧化应激损伤的保护作用[J].中医药信息,2017,34(2):9-13.
[19]李振彬,马旭,杨静,等.白芍总苷治疗大鼠急性痛风性关节炎的作用及对血清IL-1β、IL-6、TNF-α的影响[J].新医学,2017,48(6):380-384.