中药提取物“域发生发液”促进小鼠毛发生长的研究
摘要
目的观察中药提取物"域发生发液"促进小鼠毛发生长的作用及其机制。方法小鼠随机分为5个组,外用"域发生发液",与外用"米诺地尔酊"及空白组做对照,观察小鼠毛发生长的进展,同时进行HE染色及免疫组化实验进一步说明其作用机制。结果治疗后第7天,空白组小鼠毛囊仍在静止期,其余4组小鼠毛囊明显提前进入生长期;治疗后第14天,空白组小鼠毛囊才进入生长期,对每组进入生长期的毛囊进行计数统计,每个治疗组与空白组间比较有统计学意义。在毛囊生长期检测到毛发生长相关通路蛋白β-catenin及Shh蛋白的特异性表达。结论 "域发生发液"通过使静止期毛发提前进入生长期,促进毛发生长。
引文
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[2]Nguyen H,Merrill BJ,Polak L,et al.Tcf3 and Tcf4 are essential forlong-term homeostasis of skin epithelia[J].Nat Genet,2009,41(10):1068-1075.
[3]Slominski A1,Paus R,Plonka P,et al.Melanogenesis during the anagen-catagen-telogen transformation of the murine hair cycle.1994,102(6):862-869.
[4]Slominski A,Paus R.Melanogenesis is coupled to murine anagen:toward new concepts for the role of melanocytes and the regulation of melanogenesis in hair growth[J].J Invest Dermatol,1993,(101):90-97.
[5]De Villez RL.The therapeutic use of topical minoxidil[J].DermatolClin,1990,8(2):3673-3675.
[6]Nan-nan Zhang,Dong Ki Park,Hye-Jin Park.Hair growth-promoting activity of hot water extract of Thuja orientalis[J].BMC Complementary and Alternative Medicine,2013,(13):9.
[7]胡衡.獐芽菜属药用植物的生药鉴别及图像分析研究[D].湖北中医学院,2003.
[8]张晓丽,朱自平.绞股蓝总苷对小鼠微循环障碍的改善作用[J].中国农业,2005,8(5):19-20.
[9]何红梅,朱红霞,刘强,等.何首乌提取物对C57BL/6L小鼠毛囊生长和毛发生长周期的影响[J].中国实验方剂学杂志,2012,18(23):216-219.
[10]Yunfei Li,Mingnuan Han,Pei Lin,et al.Hair Growth Promotion Activity and Its Mechanism of Polygonum multiflorum[J].Evidence-Based Complementary and Alternative Medicine,2015,1-10.
[11]刘莉,陈育,张璐,等.人参提取物对C57小鼠生发作用的影响[J].中国实验方剂学杂志,2013,19(7):250-254.
[12]吴巧云,吴贤杰,吕中法,等.红花和当归等中药煎剂对体外培养的鼠毛囊毛发生长与毛球部细胞增殖的影响[J].浙江大学学报,2006,35(4):435-439.
[13]Gyeong-Hun Park,Ki-young Park,Hong-il Cho,et al.Red Ginseng Extract Promotes the Hair Growth in Cultured Human Hair Follicles[J].J Med Food,2015,18(3):354-362.
[14]Reddy S,Andl T,Bagasra A,et al.Characterization of Wnt gene expression in developing and postnatal hair follicles and identification of Wnt5a as a target of Sonichedgehog in hair follicle morphogenesis[J].Mech Dev,2001,(107):69-82.
[15]Joerg Huelsken,Regina Vogel,Erdmann B,et al.β-Catenin Controls Hair Follicle Morphogenesis and Stem Cell Differentiation in the Skin[J].Cell,2001,(105):533-545.
[16]Wang L C,Liu Z Y,Gambardella L,et al.Regular articles:conditional disruption of hedgehog signaling pathway defines its critical role in hair development and regeneration[J].J Invest Dermatol,2000,114(5):901-908.