滑子菇多糖的结构及体外生物活性探究
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摘要
以滑子菇水提醇沉多糖(PNP)为对象,通过傅里叶红外光谱(FT-IR)、核磁共振(1H NMR)、刚果红试验等试验对PNP结构进行分析。体外试验测定了PNP对脾淋巴细胞的增殖和H2O2损伤保护的作用。结构分析表明,PNP具有多糖的特征吸收峰,由4种单糖组成,以β型糖苷键为主不含乙酰基、糖醛酸的吡喃环结构,具有较长的侧链和较多的分枝结构。质量浓度为400μg/mL的PNP对淋巴细胞的增殖作用最大,增殖率为5.93%,100μg/mL及以下浓度时则表现抑制作用。质量浓度为20μg/mL的PNP对H2O2损伤的保护作用最大,保护率为54.66%。
The polysaccharide of PNP was isolated from Pholiota nameko by ethanol subsiding method.The PNP research on structure were analyzed by FT-IR,1 HNMR,Congo red,et al,and the PNP on Spleen lymphocyte proliferation and H_2O_2 damage of the cells in vitro immune activity were measured.The result showed that PNP had the typical characteristics of polysaccharides absorption peaks,and PNP was composed of four monosaccharides,and the sugar chain was mainly β-configuration pyranoside,no acetyl and uronic acid,and contained a long side chain and branches.PNP had the effect on Spleen lymphocyte proliferation,the best effect on lymphocyte proliferation with 400 μg/mL.The proliferation activity of PNP reached 5.93%,low concentration had no effect on lymphocyte proliferation,the best effect on protection cells of H_2O_2 damage with 20 μg/mL,reached the best protection activity 54.66%.
The polysaccharide of PNP was isolated from Pholiota nameko by ethanol subsiding method.The PNP research on structure were analyzed by FT-IR,1 HNMR,Congo red,et al,and the PNP on Spleen lymphocyte proliferation and H_2O_2 damage of the cells in vitro immune activity were measured.The result showed that PNP had the typical characteristics of polysaccharides absorption peaks,and PNP was composed of four monosaccharides,and the sugar chain was mainly β-configuration pyranoside,no acetyl and uronic acid,and contained a long side chain and branches.PNP had the effect on Spleen lymphocyte proliferation,the best effect on lymphocyte proliferation with 400 μg/mL.The proliferation activity of PNP reached 5.93%,low concentration had no effect on lymphocyte proliferation,the best effect on protection cells of H_2O_2 damage with 20 μg/mL,reached the best protection activity 54.66%.
引文
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[2]李海平,张树海,张坤生.滑菇多糖抗氧化活性研究[J].食品研究与开发,2008,29(4):56-60.
[3]LI H,ZHANG M,MA G.Hypolipidemic effect of the polysaccharide from Pholiota nameko[J].Nutrition,2010,26(5):556-562.
[4]WU Y,LI W,CUI W,et al.A molecular modeling approach to understand conformation-functionality relationships of galactomannans with different mannose/galactose ratios[J].Food Hydrocolloids,2012,26(2):359-364.
[5]李石军,王凯平,汪柳,等.香菇多糖LNT_2的提取分离纯化,结构及体外抗肿瘤活性研究[J].中草药,2014,45(9):1232-1237.
[6]李蓉,孙书娟,江晓路.虫草多糖的分离纯化及结构鉴定[J].中国药师,2015,18(3):407-409,419.
[7]史晨杉,孙桂清,张亚卓,等.碱处理对龙须菜非琼胶多糖的提取及体外免疫活性的影响[J].食品科技,2015,40(3):196-199.
[8]朱科学,聂少平,李文娟,等.黑灵芝多糖对小鼠脾淋巴细胞增殖及诱生细胞因子的影响[J].食品科学,2010,31(19):351-354
[9]章世元,徐春燕,董晓芳,等.苜蓿多糖和黄芪多糖对肉仔鸡淋巴细胞增殖的影响[J].动物营养学报,2010,22(3):670-674.
[10]李雪丹.滑子菇不同潮期营养成分的测定及其多糖的提取纯化研究[D].石家庄:河北农业大学,2015.
[11]YAN C,YIN Y,ZHANG D,et al.Structural characterization and in vitro antitumor activity of a novel polysaccharide from Taxus yunnanensis[J].Carbohydrate Polymers,2013,96(2):389-395.
[12]LIU X,WANG L,ZHANG C,et al.Structure characterization and antitumor activity of a polysaccharide from the alkaline extract of king oyster mushroom[J].Carbohydrate Polymers,2015,118:101-106.
[13]张卓然.培养细胞学与细胞培养技术[M].上海:上海科学技术出版社,2004:39-41,92-94.
[14]CHEN J R,YANG Z Q,HU T J,et al.Immunomodulatory activity in vitro and in vivo of polysaccharide from Potentilla anserina[J].Fitoterapia,2010,81(8):1117-1124.
[15]张元,林强,宋玥.壳寡糖及其磷酸化修饰产物对小鼠脾淋巴细胞增殖及诱生细胞因子的影响[J].食品科技,2012,37(2):11-15.
[16]WEI J,WANG S,LIU G,et al.Polysaccharides from Enteromorpha prolifera enhance the immunity of normal mice[J].International Journal of Biological Macromolecules,2014,64:1-5.
[17]刘晶.Viili中乳酸菌的分离及其胞外多糖的研究[D]石家庄:河北农业大学,2015.
[18]张惟杰.糖复合物生化研究技术[M].2版.杭州:浙江大学出版社,1999:197-203.
[19]ROUT D,MONDAL S,CHAKRABORTY I,et al The structure and conformation of a water-insoluble(1→3)-,(1→6)-β-d-glucan from the fruiting bodies of Pleurotus florida[J].Carbohydrate Research,2008,343(5):982-987.
[20]CHO C W,HAN C,RHEE Y K,et al.Cheonggukjang polysaccharides enhance immune activities and prevent cyclophosphamide-induced immunosuppression[J].International Journal of Biological Macromolecules,2015,72:519-525.
[21]段小群,焦杨,张士军,等.玉郎伞多糖对过氧化氢诱导大鼠原代肝细胞损伤的保护作用[J].时珍国医国药,2007,18(7):1592-1593.
[22]SONG G,DU Q.Structure characterization and antitumor activity of anαβ-glucan polysaccharide from Auricularia polytricha[J].Food Research International,2012,45(1):381-387.