党参多糖和黄芪多糖对鸡球虫疫苗免疫增强作用的研究
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摘要
为研究党参多糖(codonopsis pilosula polysaccharides,CPP)和黄芪多糖(astragalus polysaccharides,APS)对鸡球虫四价五株早熟弱毒活疫苗的免疫增强作用,采用免疫攻虫试验,对添加和不添加CPP和APS的鸡球虫疫苗免疫攻虫鸡的增重和免疫效果进行比较。结果表明:二次免疫后,添加CPP和APS免疫组鸡的相对增重率均高于不加药组;中剂量CPP和高剂量APS(25.0 mg/L和14.4 mg/L,T6组)在攻虫后相对增重率(95.85%)大于不加药免疫组,相比其余加药免疫组最高;T6组在三个肠段的病变记分中均最低,盲肠病变记分显著低于不加药免疫组(P<0.05),但在十二指肠的病变记分和空肠的病变记分相比不加药免疫组差异不显著(P>0.05);T6组三个肠段的病变记分减少率最高,分别为十二指肠83.87%、空肠86.36%和盲肠80.00%。说明中剂量CPP和高剂量APS可以缓解鸡球虫疫苗免疫对鸡增重的不良影响,提高鸡球虫疫苗的免疫效果。
The aim of the present study was to investigate the immune enhancement effect of codonopsis pilosula polysaccharides( CPP) and astragalus polysaccharides( APS) on five strains of eimeria quadrivalence immune synergism precocious vaccine. Chicken coccidiosis vaccine with or without CPP and APS was used to immunize the chicks. Then the weight gain and immune effect of the challenged chickens were compared by using the immune challenge test. The results showed that the relative weight gain rate of chickens immunized with CPP and APS was higher than that of the control group after secondary immunization. The relative weight gain rate( RWR) of middle dose CPP and high dose APS( T6)group was 95. 85%,which was higher than that of the control group,and was the highest compared with the other groups. The lesion score of T6 group was the lowest among the three intestinal segments,and the score of caecum lesion was significantly lower than that of the control group( P<0. 05),but the score of duodenal lesion and jejunal lesion was not significantly different from that of the control group( P>0. 05). In the T6 group,the lesion score reduction rate of the three intestinal segments was the highest( 83. 87% in duodenum,86. 36% in jejunum and80. 00% in cecum). The results indicated that middle dose CPP and high dose APS could alleviate the adverse effect of chicken coccidiosis vaccine immunization on chicken weight gain and improve the immune effect of chicken coccidiosis vaccine.
The aim of the present study was to investigate the immune enhancement effect of codonopsis pilosula polysaccharides( CPP) and astragalus polysaccharides( APS) on five strains of eimeria quadrivalence immune synergism precocious vaccine. Chicken coccidiosis vaccine with or without CPP and APS was used to immunize the chicks. Then the weight gain and immune effect of the challenged chickens were compared by using the immune challenge test. The results showed that the relative weight gain rate of chickens immunized with CPP and APS was higher than that of the control group after secondary immunization. The relative weight gain rate( RWR) of middle dose CPP and high dose APS( T6)group was 95. 85%,which was higher than that of the control group,and was the highest compared with the other groups. The lesion score of T6 group was the lowest among the three intestinal segments,and the score of caecum lesion was significantly lower than that of the control group( P<0. 05),but the score of duodenal lesion and jejunal lesion was not significantly different from that of the control group( P>0. 05). In the T6 group,the lesion score reduction rate of the three intestinal segments was the highest( 83. 87% in duodenum,86. 36% in jejunum and80. 00% in cecum). The results indicated that middle dose CPP and high dose APS could alleviate the adverse effect of chicken coccidiosis vaccine immunization on chicken weight gain and improve the immune effect of chicken coccidiosis vaccine.
引文
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[3]曹丽,罗崇念,卞庆亚,等.党参多糖对鸡IL-2活性和淋巴细胞增殖反应的促进作用[J].中兽医医药杂志,2004,23(1):3-4.
[4]司昌德,闵亚宏.黄芪多糖对肉仔鸡免疫功能的影响[J].中国预防兽医学报,2008,30(12):978-980.
[5]周建强,潘琦,王涛,等.党参多糖对免疫雏鸡抗体效价和淋巴细胞增殖的影响[J].江苏农业科学,2010(3):263-264.
[6]罗燕,邵永斌,谷新利,等.黄芪多糖佐剂对鸡禽流感-新城疫重组二联苗免疫效果的影响[J].中兽医医药杂志,2008,27(5):13-15.
[7]吕强华.鸡球虫病四价五株活疫苗实验室效力和安全性研究[D].太谷:山西农业大学,2016.
[8]索勋,李国清.鸡球虫病学[M].北京:中国农业大学出版社,1998.
[9]王乐元.兽用生物制品质量标准汇编[M].北京:中国农业出版社,2013.
[10]李达,李在林.党参多糖作为免疫佐剂的研究综述[J].中国医药指南,2013,11(28):56-57.
[11]周金星,李升和,宁康健,等.不同剂量党参、淫羊藿提取物对青脚麻鸡盲肠组织结构的影响[J].安徽科技学院学报,2012,26(1):14-18.
[12]周建强,潘琦,王涛,等.党参多糖对免疫雏鸡抗体效价和淋巴细胞增殖的影响[J].江苏农业科学,2010(3):263-264.
[13]石轶男,杨绒娟,扆妍妍,等.党参多糖可溶性粉对肉仔鸡血清ND抗体水平、Ig G及肠道SIg A含量的影响[J].中国兽药杂志,2016,50(9):47-52.
[14]张伟,朱连勤,王吉才,等.黄芪多糖在增强家禽免疫功能和抗病毒方面的应用[J].家禽科学,2007(1):41-43.
[15]樊福好,谢明权.免疫调节剂与抗球虫药联合应用的抗球虫效果[J].中国动物传染病学报,2000,8(3):1-3.