硫营养对春油菜幼苗生长及生理生化指标的影响
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摘要
为了研究硫对春油菜幼苗生长及生理生化指标的影响,以春油菜品种"青杂5号"为试材,采用单因素完全随机设计盆栽试验,研究6个不同施硫量(0,3.3,6.6,13.3,20,26.6 mg/kg)对春油菜幼苗的生长和叶片生理生化指标的影响。结果表明:随着施硫量的增加,叶片MDA含量逐渐升高,SOD活性逐渐下降,其他农艺性状和生理生化指标均呈先升高后降低趋势;当施硫量为6.6 mg/kg时,幼苗植株干重、根长、根系活力、叶绿素b、叶绿素a+b、类胡萝卜素、可溶性糖、可溶性蛋白和硝酸还原酶活性均显著高于对照(P<0.05);当施硫量为20.0 mg/kg时,POD活性达到最高,显著高于对照71.15%(P<0.05),MDA含量在施硫量为3.3 mg/kg时达到最低,显著低于对照52.5%(P<0.05)。因此,施硫量为3.3～6.6 mg/kg时可明显促进春油菜幼苗生长和提高幼苗叶片相关酶活性。
The present study evaluated the effects of different sulfur application on the growth and physiological and biochemical indicators of spring rape seedlings. Cultivar 'Qingza 5 was used as testing material,testing was designed randomly with single factor and 6 sulfur fertilizer levels(0,3.3,6.6,13.3,20,26.6 mg/kg) were set. The results showed that with the increase of sulfur application,the content of MDA increased gradually and the activity of SOD decreased gradually. The agronomic characters and physiological and biochemical indicator showed the increasing first and decreasing later. When the sulfur application was 6.6 mg/kg,the dry weight,root length,root activity,chlorophyll b,chlorophyll a+b,carotenoids,soluble sugar,soluble protein and NR activity were significantly higher than CK(P<0.05). When the sulfur content was 20.0 mg/kg,the activity of POD was the highest,which was 71.15% higher than that of CK(P<0.05). When the sulfur application was 3.3 mg/kg,MDA content was the lowest,which was 52.5% lower than that of CK(P<0.05). Sulfur application with 3.3～6.6 mg/kg would promote the growth of spring rape seedlings significantly and the enzyme activity of leaves would be effectively improved.
The present study evaluated the effects of different sulfur application on the growth and physiological and biochemical indicators of spring rape seedlings. Cultivar 'Qingza 5 was used as testing material,testing was designed randomly with single factor and 6 sulfur fertilizer levels(0,3.3,6.6,13.3,20,26.6 mg/kg) were set. The results showed that with the increase of sulfur application,the content of MDA increased gradually and the activity of SOD decreased gradually. The agronomic characters and physiological and biochemical indicator showed the increasing first and decreasing later. When the sulfur application was 6.6 mg/kg,the dry weight,root length,root activity,chlorophyll b,chlorophyll a+b,carotenoids,soluble sugar,soluble protein and NR activity were significantly higher than CK(P<0.05). When the sulfur content was 20.0 mg/kg,the activity of POD was the highest,which was 71.15% higher than that of CK(P<0.05). When the sulfur application was 3.3 mg/kg,MDA content was the lowest,which was 52.5% lower than that of CK(P<0.05). Sulfur application with 3.3～6.6 mg/kg would promote the growth of spring rape seedlings significantly and the enzyme activity of leaves would be effectively improved.
引文
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[14] 高中超,张喜林,马星竹.植株体内硫素的生理功能及作用研究进展[J].黑龙江农业科学,2009(5):153-155.
[15] 周莉娜,曲东,邵丽丽,等.干旱胁迫下硫营养对小麦光合色素及MDA含量的影响[J].西北植物学报,2005,25(8):1579-1583.
[16] 徐瑶,何玲莉,黄思杰,等.施硫方式及时期对不结球白菜营养品质与光合特性的影响[J].江苏农业科学,2017,45(23):115-120.
[17] 盛艳敏,尹金植,石德成,等.盐碱协同胁迫对向日葵抗氧化酶系统的影响[J].中国生物化学与分子生物学报,2008,24(8):704-711.
[18] 曲东,邵丽丽,王保莉,等.干旱胁迫下硫对玉米叶绿素及MDA含量的影响[J].干旱地区农业研究,2004,22(2):91-94.
[19] 朱英华,屠乃美,肖汉乾,等.硫对烤烟生长发育及生理生化指标的影响[J].中国烟草学报,2008,4(14):28-30.
[20] 李金凤,陈洪斌,张玉龙,等.辽宁大豆主产区土壤硫素状况及不同硫肥肥效研究[J].土壤通报,2004,35(4):470-473.
[21] 许建,贾凯,朱君芳,等.氮硫互作提高大蒜氮、硫含量及其关键同化酶活性[J].植物营养与肥料学报,2017,23(2):434-443 .
[22] 刘开昌,李爱芹.施硫对高油、高淀粉玉米品质的影响及生理生化特性[J].玉米科学,2004,12(专刊):111-113.
[2] 杜德志,肖麓,赵志,等.我国春油菜遗传育种研究进展[J].中国油料作物学报,2018,40(5):633-639.
[3] 郑诗樟.硫肥对土壤性质、重金属形态和作物生长的影响[D].武汉:华中农业大学,2012.
[4] 张毅,卢俊宇,骆玉林,等.硫肥对油菜生长及产量的影响[J].四川农业科技,2014(6):47-48.
[5] DAWOOD M.硫化氢缓解铝、镉和盐胁迫及硫营养影响大麦生长与品质的生理机制[D].杭州:浙江大学,2012.
[6] 王庆仁,林葆.硫胁迫对油菜超微结构及超细胞水平硫分布的影响[J].植物营养与肥料学报,1999,5(1):46-49.
[7] 吴宇,高蕾,曹民杰,等.植物硫营养代谢、调控与生物学功能[J].植物学报,2007,24(6):735-761.
[8] 祝小捷.氮硫配施对小麦产量和品质的调控及其生理学基础[D].郑州:河南农业大学,2008.
[9] 成凯.硒硫互作对青花菜幼苗生理及光合特性的影响[D].成都:四川农业大学,2016.
[10] 白灯莎·买买提艾力,阿依夏木,徐舫,等.3种含硫肥料对油菜干物质累积、氮硫吸收及产量的影响[J].中国油料作物学报,2009,31(1):86-89.
[11] 崔岩山,王庆仁.不同种类硫肥对油菜吸锌的影响[J].中国生态农业学报,2008,16(1):113-116.
[12] KATARZYNA P,BOGDAN K,WIKTOR H,et al.Response of oilseed rape leaves to sulfur and boron foliar application[J].ACTA Physiol Plant,2018,40:169.
[13] 苍晶,赵会杰.植物生理学试验教程[M].北京:高等教育出版社,2014:145-153.
[13] 田华,段美洋,王兰.植物硝酸还原酶功能的研究进展[J].中国农学通报,2009,25(10):96-99.
[14] 高中超,张喜林,马星竹.植株体内硫素的生理功能及作用研究进展[J].黑龙江农业科学,2009(5):153-155.
[15] 周莉娜,曲东,邵丽丽,等.干旱胁迫下硫营养对小麦光合色素及MDA含量的影响[J].西北植物学报,2005,25(8):1579-1583.
[16] 徐瑶,何玲莉,黄思杰,等.施硫方式及时期对不结球白菜营养品质与光合特性的影响[J].江苏农业科学,2017,45(23):115-120.
[17] 盛艳敏,尹金植,石德成,等.盐碱协同胁迫对向日葵抗氧化酶系统的影响[J].中国生物化学与分子生物学报,2008,24(8):704-711.
[18] 曲东,邵丽丽,王保莉,等.干旱胁迫下硫对玉米叶绿素及MDA含量的影响[J].干旱地区农业研究,2004,22(2):91-94.
[19] 朱英华,屠乃美,肖汉乾,等.硫对烤烟生长发育及生理生化指标的影响[J].中国烟草学报,2008,4(14):28-30.
[20] 李金凤,陈洪斌,张玉龙,等.辽宁大豆主产区土壤硫素状况及不同硫肥肥效研究[J].土壤通报,2004,35(4):470-473.
[21] 许建,贾凯,朱君芳,等.氮硫互作提高大蒜氮、硫含量及其关键同化酶活性[J].植物营养与肥料学报,2017,23(2):434-443 .
[22] 刘开昌,李爱芹.施硫对高油、高淀粉玉米品质的影响及生理生化特性[J].玉米科学,2004,12(专刊):111-113.