样本溶血对血浆中16种氨基酸测定准确性的探究
摘要
为探究样本溶血对国标酸解法测定血浆中16种氨基酸含量的影响,采集60份断奶仔猪颈动脉血液并分为3组,即普通溶血组、机械溶血组与对照组,普通溶血组为血液采集后溶血样本,机械溶血组为血液采集后经超声波处理溶血再离心样本,未溶血血浆为对照组。采用赛卡姆S433D全自动氨基酸分析仪检测血浆样本中天冬氨酸(Asp)、苏氨酸(Thr)、丝氨酸(Ser)、谷氨酸(Glu)、甘氨酸(Gly)、丙氨酸(Ala)、缬氨酸(Val)、蛋氨酸(Met)、异亮氨酸(Ile)、亮氨酸(Leu)、酪氨酸(Tyr)、苯丙氨酸(Phe)、脯氨酸(Pro)、赖氨酸(Lys)、组氨酸(His)、精氨酸(Arg)的含量。结果表明,普通溶血组Asp、Ala、Val、Leu、His、Lys含量相较于其余两组显著升高(P<0.05),其余10种氨基酸含量相较于对照组偏高或偏低(P>0.05)。说明血样溶血对其氨基酸含量存在较大影响,且受非血红蛋白因素影响较小,为了尽可能避免或减少溶血的发生,建议采血操作以及样品的运送、分离检测严格规范,以提高测定的准确性。
引文
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[3]张春燕,程昱璇,赵振宇,等.基于代谢组学评价血液生物标志物对中枢神经系统肿瘤的诊断价值[J].标记免疫分析与临床,2017,24(6):601-605.
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[6]蔡万存,李向飞,蒋广震.大米蛋白替代鱼粉对团头鲂生长、肠道消化吸收功能以及氨基酸代谢的影响[J].南京农业大学学报,2017(3):529-538.
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[8]谢绍霞.溶血对生化检验结果准确性的影响及校正方法分析[J].中外女性健康研究,2018,9(17):111,166.
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[13]刘小娟,杜晓琳,黄娟,等.生物分析中溶血对检测的影响及其对策[J].中国新药与临床杂志,2017(4):7-11.
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[2]周艳和.新生儿疾病筛查足跟采血法的护理研究进展[J].中西医结合护理(中英文),2016,2(6):217-219.
[3]张春燕,程昱璇,赵振宇,等.基于代谢组学评价血液生物标志物对中枢神经系统肿瘤的诊断价值[J].标记免疫分析与临床,2017,24(6):601-605.
[4]修风民,张凤,矫健鹏,等.胃癌患者血浆24种氨基酸代谢物水平变化[J].第二军医大学学报,2018,39(1):62-67.
[5]祝倩,查伟,姬玉娇.母猪饲粮添加脯氨酸对哺乳环江香猪血浆生化参数和氨基酸含量的影响[J].华南农业大学学报,2018,38(1):7-11..
[6]蔡万存,李向飞,蒋广震.大米蛋白替代鱼粉对团头鲂生长、肠道消化吸收功能以及氨基酸代谢的影响[J].南京农业大学学报,2017(3):529-538.
[7]吴小平.探讨溶血对临床生化检验结果的影响[J].中国医药指南,2018(10):42-43.
[8]谢绍霞.溶血对生化检验结果准确性的影响及校正方法分析[J].中外女性健康研究,2018,9(17):111,166.
[9]赵东.溶血对生化检验结果准确性的影响及校正方法分析[J].检验医学与临床,2012,9(16):2024-2025.
[10]陈红琴.溶血对生化检验准确性的影响及纠正探析[J].现代诊断与治疗,2014,1(2):387.
[11]蔡君.猪血生物法制备血红素及珠蛋白肽的工艺研究[D].武汉:湖北工业大学,2013.
[12]GB/T 18246-2000饲料中氨基酸的测定[S].
[13]刘小娟,杜晓琳,黄娟,等.生物分析中溶血对检测的影响及其对策[J].中国新药与临床杂志,2017(4):7-11.
[14]LAGA A,CHEVES T,MAROTO S,et al.The suitability of hemolyzed specimens for compatibility testing using automated technology[J].Transfusion,2008,48(8):8.
[15]WU G,BAZER F W,DAI Z,et al.Amino acid nutrition in animals:Protein synthesis and beyond[J].Annual Review of Animal Biosciences,2014,2(1):387.
[16]FAN Q,YAN X,YIN Y,et al.Amino Acids,Gene Expression,and Cell Signaling in the Pig Intestine[C].Nutritional and Physiological Functions of Amino Acids in Pigs[M].Vienna:Springer,2013.
[17]MAVROMICHALIS I,KERR B J,PARR T M,et al.Valine requirement of nursery pigs[J].Journal of Animal Science,2001,79(5):1223-1229.
[18]ESCOBAR J,FRANK J W,SURYAWAN A,et al.Physiological rise in plasma leucine stimulates muscle protein synthesis in neonatal pigs by enhancing translation initiation factor activation[J].American Journal of Physiology Endocrinology&Metabolism,2005,288(5):E914-921..
[19]PAN L,MA X K,WANG H L,et al.Enzymatic feather meal as an alternative animal protein source in diets for nursery pigs[J].Animal Feed Science&Technology,2016,212:112-121.
[20]ROMAN Y,BOMSEL-DEMONTOY M C,LEVRIER J,et al.Effect of hemolysis on plasma protein levels and plasma electrophoresis in birds[J].Journal of Wildlife Diseases,2009,45(1):73-80.
[21]TAN A,GAGN魪S,L魪VESQUE I A,et al.Impact of hemolysis during sample collection:How different is drug concentration in hemolyzed plasma from that of normal plasma?[J].Journal of Chromatography B,Analytical Technologies in the Biomedical and Life Sciences,2012,901(none):79-84.
[22]TANG D,THOMAS E.Strategies for dealing with hemolyzed samples in regulated LC-MS/MS bioanalysis[J].Bioanalysis,2012,4(22):2715-2724.