摘要
目的探讨含微乳头结构(MPP)肺腺癌具有的CT重建征象特征,上皮间质转化(EMT)相关分子表达情况以及它们之间的关系。方法选取含MPP侵袭性肺腺癌(IAC) 37例、不含MPP 80例及其对应的癌旁组织,比较临床病理特征、CT重建征象;应用Real-time PCR及Western blotting检测EMT有关标识分子表达变化情况。结果含MPP组淋巴结转移比例较高、毛刺、胸膜凹陷征、实性及分叶比例较高(均P<0.001)。含MPP组E-钙黏蛋白(cadherin)及β-连环蛋白(β-catenin)表达均下调(P<0.05),N-cadherins、波形蛋白(vimentin)、Snail及转化生长因子(TGF)-β表达均上调(P<0.05)。有毛刺组E-cadherin及β-catenin表达下调者所占比例较高,而vimentin表达上调者所占比例较高(均P<0.05);实性结节组E-cadherin表达下调者所占比例较高,而N-cadherin及vimentin表达上调者所占比例较高(均P<0.05)。结论含MPP浸润性肺腺癌具有特征性CT重建征象,发生了EMT改变。其CT重建征象与EMT相关分子表达存在联系。
Objective To investigate the features of CT reconstruction imags,expressions of genes associated with epithelial-mesenchymal transition(EMT) in lung adenocarcinoma with micropapillary pattern(MPP) and the relationships between them.Methods Thirty-seven cases of invasive lung invasive adenocarcinoma(IAC) with MPP,80 cases without MPP and their corresponding para-cancerous tissues were selected.The clinicopathological features and features of CT reconstruction images were compared.Real-time PCR and Western blotting were used to detect the expression of EMT markers.Results In MPP group,the proportions of lymph node metastasis,spicule,pleural indentation,solid type and lobulation were higher(P < 0.001).In MPP group,the expressions of E-cadherin,β-catenin were decreased(P < 0.05),and the expressions of N-cadherin,vimentin,Snail and transforming growth factor(TGF)-β were increased(P<0.05).In spicule group,the percentage of decreased expression of E-cadherin and β-catenin was higer,while the percentage of increased expression of vimentin was higher(P<0.05).In the group of solid type,the percentage of decreased expression of E-cadherin was higher,while the percentages of increased expression of N-cadherin and vimentin were higher(P< 0.05).Conclusion Lung invasive adenocarcinoma has characteristics of CT reconstruction images and EMT changes.CT reconstruction images are associated with the expression of the related molecules associated of EMT.
引文
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