含微乳头结构肺腺癌CT重建征象特征及上皮间质转化相关基因表达
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摘要
目的探讨含微乳头结构(MPP)肺腺癌具有的CT重建征象特征,上皮间质转化(EMT)相关分子表达情况以及它们之间的关系。方法选取含MPP侵袭性肺腺癌(IAC) 37例、不含MPP 80例及其对应的癌旁组织,比较临床病理特征、CT重建征象;应用Real-time PCR及Western blotting检测EMT有关标识分子表达变化情况。结果含MPP组淋巴结转移比例较高、毛刺、胸膜凹陷征、实性及分叶比例较高(均P<0.001)。含MPP组E-钙黏蛋白(cadherin)及β-连环蛋白(β-catenin)表达均下调(P<0.05),N-cadherins、波形蛋白(vimentin)、Snail及转化生长因子(TGF)-β表达均上调(P<0.05)。有毛刺组E-cadherin及β-catenin表达下调者所占比例较高,而vimentin表达上调者所占比例较高(均P<0.05);实性结节组E-cadherin表达下调者所占比例较高,而N-cadherin及vimentin表达上调者所占比例较高(均P<0.05)。结论含MPP浸润性肺腺癌具有特征性CT重建征象,发生了EMT改变。其CT重建征象与EMT相关分子表达存在联系。
Objective To investigate the features of CT reconstruction imags,expressions of genes associated with epithelial-mesenchymal transition(EMT) in lung adenocarcinoma with micropapillary pattern(MPP) and the relationships between them.Methods Thirty-seven cases of invasive lung invasive adenocarcinoma(IAC) with MPP,80 cases without MPP and their corresponding para-cancerous tissues were selected.The clinicopathological features and features of CT reconstruction images were compared.Real-time PCR and Western blotting were used to detect the expression of EMT markers.Results In MPP group,the proportions of lymph node metastasis,spicule,pleural indentation,solid type and lobulation were higher(P < 0.001).In MPP group,the expressions of E-cadherin,β-catenin were decreased(P < 0.05),and the expressions of N-cadherin,vimentin,Snail and transforming growth factor(TGF)-β were increased(P<0.05).In spicule group,the percentage of decreased expression of E-cadherin and β-catenin was higer,while the percentage of increased expression of vimentin was higher(P<0.05).In the group of solid type,the percentage of decreased expression of E-cadherin was higher,while the percentages of increased expression of N-cadherin and vimentin were higher(P< 0.05).Conclusion Lung invasive adenocarcinoma has characteristics of CT reconstruction images and EMT changes.CT reconstruction images are associated with the expression of the related molecules associated of EMT.
Objective To investigate the features of CT reconstruction imags,expressions of genes associated with epithelial-mesenchymal transition(EMT) in lung adenocarcinoma with micropapillary pattern(MPP) and the relationships between them.Methods Thirty-seven cases of invasive lung invasive adenocarcinoma(IAC) with MPP,80 cases without MPP and their corresponding para-cancerous tissues were selected.The clinicopathological features and features of CT reconstruction images were compared.Real-time PCR and Western blotting were used to detect the expression of EMT markers.Results In MPP group,the proportions of lymph node metastasis,spicule,pleural indentation,solid type and lobulation were higher(P < 0.001).In MPP group,the expressions of E-cadherin,β-catenin were decreased(P < 0.05),and the expressions of N-cadherin,vimentin,Snail and transforming growth factor(TGF)-β were increased(P<0.05).In spicule group,the percentage of decreased expression of E-cadherin and β-catenin was higer,while the percentage of increased expression of vimentin was higher(P<0.05).In the group of solid type,the percentage of decreased expression of E-cadherin was higher,while the percentages of increased expression of N-cadherin and vimentin were higher(P< 0.05).Conclusion Lung invasive adenocarcinoma has characteristics of CT reconstruction images and EMT changes.CT reconstruction images are associated with the expression of the related molecules associated of EMT.
引文
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[11]Zhou S,Sun X,Yu L,et al.Differential expression and clinical significance of epithelial-mesenchymal transition markers among different histological types of triple-negative breast cancer[J].JCancer,2018,9(3):604-613.
[12]Cai YR,Dong YJ,Wu HB,et al.Micropapillary:a component more likely to harbour heterogeneous EGFR mutations in lung adenocarcinomas[J].Sci Rep,2016,6:23755.
[13]Zhang Y,Wang R,Cai D,et al.A comprehensive investigation of molecular features and prognosis of lung adenocarcinoma with micropapillary component[J].J Thorac Oncol,2014,9(12):1772-1778.
[14]Dai C,Xie H,Kadeer X,et al.Relationship of lymph node micrometastasis and micropapillary component and their joint influence on prognosis of patients with stageⅠlung adenocarcinoma[J].Am J Surg Pathol,2017,41(9):1212-1220.
[15]Kawakami T,Nabeshima K,Makimoto Y,et al.Micropapillary pattern and grade of stromal invasion in p T1 adenocarcinoma of the lung:usefulness as prognostic factors[J].Mod Pathol,2007,20(5):514-521.
[16]Lee HJ,Eom DW,Kang GH,et al.Colorectal micropapillary carcinomas are associated with poor prognosis and enriched in markers of stem cells[J].Mod Pathol,2013,26(8):1123-1131.
[17]Hong M,Kim JW,Shin MK,et al.Poorly differentiated clusters in colorectal adenocarcinomas share biological similarities with micropapillary patterns as well as tumor buds[J].J Korean Med Sci,2017,32(10):1595-1602.
[18]Ruparel M,Quaife SL,Navani N,et al.Pulmonary nodules and CT screening:the past,present and future[J].Thorax,2016,71(4):367-375.
[2]Wang ShJ.Value of multi-slice spiral CT reconstruction in diagnosis of peripheral lung cancer[J].China Modern Doctor,2011,49(23):68-69.(in Chinese)王善军.多层螺旋CT重建技术在周围型肺癌诊断中的价值[J].中国现代医生,2011,49(23):68-69.
[3]Fu YS,Hao AG,Zhou WY,et al.Value of diagnosis with multislice spiral CT in peripheral small lung carcinoma[J].Journal of Medical Imaging,2008,18(8):855-858.(in Chinese)傅延森,郝爱国,周万勇,等.多层螺旋CT薄层扫描MPR重建对周围型小肺癌的诊断价值[J].医学影像学杂志,2008,18(8):855-858.
[4]Yan QH,LüYG,Bao JH,et al.Small peripheral lung cancer diagnostic with MSCT's post-processing techniques[J].Hebei Medical Journal.2009,31(2):143-144.(in Chinese)闫巧焕,吕英刚,暴军辉,等.多层螺旋CT重建术在周围型小肺癌诊断中的应用[J].河北医药,2009,31(2):143-144.
[5]Brambilla E,Gazdar A.Pathogenesis of lung cancer signalling pathways:roadmap for therapies[J].Eur Resp Jo,2009,33(6):1485-1497.
[6]Piva F,Giulietti M,Santoni M,et al.Epithelial to mesenchymal transition in renal cell carcinoma:implications for cancer therapy[J].Mol Diagn Ther,2016,20(2):111-117.
[7]Lin X,Wang Zh Y,Wu JF,et al.Effect and mechanism of trefoil factor 3 on epithelial-mesenchymal transition of papillary thyroid carcinoma TPC-1 cells[J].Acta Anatomica Sinica,2018,49(2):204-211.(in Chinese)林旭,王志勇,吴靖芳,等.三叶因子3在甲状腺乳头状癌TPC-1细胞上皮间质转化中的作用及机制[J].解剖学报,2018,49(2):204-211.
[8]Cai HK,Chen X,Tang YH,et al.MicroRNA-194 modulates epithelial-mesenchymal transition in human colorectal cancer metastasis[J].Onco Targets Thera,2017,10:1269-1278.
[9]Liu J,Li W,Liu S,et al.Knockdown of collagen triple helix repeat containing 1(CTHRC1)inhibits epithelial-mesenchymal transition and cellular migration in glioblastoma Cells[J].Oncol Res,2017,26;25(2):225-232.
[10]Du L,Ning Z,Zhang H,et al.Corepressor metastasis-associated protein 3 modulates epithelial-to-mesenchymal transition and metastasis[J].Chin J Cancer,2017,36(1):28.
[11]Zhou S,Sun X,Yu L,et al.Differential expression and clinical significance of epithelial-mesenchymal transition markers among different histological types of triple-negative breast cancer[J].JCancer,2018,9(3):604-613.
[12]Cai YR,Dong YJ,Wu HB,et al.Micropapillary:a component more likely to harbour heterogeneous EGFR mutations in lung adenocarcinomas[J].Sci Rep,2016,6:23755.
[13]Zhang Y,Wang R,Cai D,et al.A comprehensive investigation of molecular features and prognosis of lung adenocarcinoma with micropapillary component[J].J Thorac Oncol,2014,9(12):1772-1778.
[14]Dai C,Xie H,Kadeer X,et al.Relationship of lymph node micrometastasis and micropapillary component and their joint influence on prognosis of patients with stageⅠlung adenocarcinoma[J].Am J Surg Pathol,2017,41(9):1212-1220.
[15]Kawakami T,Nabeshima K,Makimoto Y,et al.Micropapillary pattern and grade of stromal invasion in p T1 adenocarcinoma of the lung:usefulness as prognostic factors[J].Mod Pathol,2007,20(5):514-521.
[16]Lee HJ,Eom DW,Kang GH,et al.Colorectal micropapillary carcinomas are associated with poor prognosis and enriched in markers of stem cells[J].Mod Pathol,2013,26(8):1123-1131.
[17]Hong M,Kim JW,Shin MK,et al.Poorly differentiated clusters in colorectal adenocarcinomas share biological similarities with micropapillary patterns as well as tumor buds[J].J Korean Med Sci,2017,32(10):1595-1602.
[18]Ruparel M,Quaife SL,Navani N,et al.Pulmonary nodules and CT screening:the past,present and future[J].Thorax,2016,71(4):367-375.