红外光谱三级鉴别法联合高效液相色谱-质谱法研究不同采收期黄芩药材的质量
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摘要
目的:研究不同生长期春秋两季采收黄芩药材中有效成分种类及含量的差异,为确定黄芩的最佳采收期提供技术支持。方法:对赤峰产黄芩不同采收期药材样品(一年、二年、三年生长期,在各年份的春季和秋季分别采挖),应用红外光谱三级鉴别技术(IR-TL)分析其整体成分构成变化,以高效液相色谱-质谱联用技术(HPLC-MS)分析药材中6种黄酮类成分(千层纸素A、白杨素、黄芩素、汉黄芩素、黄芩苷、汉黄芩苷)含量的变化。结果:IR-TL分析结果显示,不同生长期黄芩所含黄酮类、苷类、糖类、酯类等成分均有所不同,其中二年生黄芩中的苷类物质含量最高、药材质量更稳定;6种黄酮类成分含量测定结果显示,不同采收期对上述指标成分含量有较大影响,其中二年生春季采收样品的整体含量高于同年份秋季采收样品。结论:本研究可为黄芩药材质量的控制提供可靠的分析方法;二年生长期春季采收可能为黄芩的最佳采收时间。
OBJECTIVE:To study effective component kinds and content difference of Scutellaria baicalensis with different harvesting periods collected in spring and autumn,and to determine the optimal harvesting time. METHODS:IR-IL was used to analyze the constitutions change of the components in S. baicalensis collected in different harvesting periods(annual,biennial and triennial,in spring and autumn,respectively). HPLC-MS method was used to analyze the contents change of 6 flavonoids(qroxylin A, chrysin, wogonin, wogonoside, baicalin, baicalein). RESULTS: Results of IR-TL analysis showed that S.baicalensis contained different components such as flavonoids,glycosides,saccharides,easters in different growth stages,among which the contents of glycosides in biennial S. baicalensis were the highest with more stable quality. The results of content determination of 6 flavonoids showed that different harvesting periods had a great influence on the contents of the above indicators.The overall content of biennial samples collected in spring was higher than that of autumn samples collected in the same year.CONCLUSIONS:This study provides a reliable analytical method for the quality control of S. baicalensis;spring harvest in two-year growth period may be the best harvesting time for S. baicalensis.
OBJECTIVE:To study effective component kinds and content difference of Scutellaria baicalensis with different harvesting periods collected in spring and autumn,and to determine the optimal harvesting time. METHODS:IR-IL was used to analyze the constitutions change of the components in S. baicalensis collected in different harvesting periods(annual,biennial and triennial,in spring and autumn,respectively). HPLC-MS method was used to analyze the contents change of 6 flavonoids(qroxylin A, chrysin, wogonin, wogonoside, baicalin, baicalein). RESULTS: Results of IR-TL analysis showed that S.baicalensis contained different components such as flavonoids,glycosides,saccharides,easters in different growth stages,among which the contents of glycosides in biennial S. baicalensis were the highest with more stable quality. The results of content determination of 6 flavonoids showed that different harvesting periods had a great influence on the contents of the above indicators.The overall content of biennial samples collected in spring was higher than that of autumn samples collected in the same year.CONCLUSIONS:This study provides a reliable analytical method for the quality control of S. baicalensis;spring harvest in two-year growth period may be the best harvesting time for S. baicalensis.
引文
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[13]张春晖,张贵君,孙素琴,等.黄芩及其提取物红外光谱与二维相关光谱的鉴别[J].光谱学与光谱分析,2010,30(7):1774-1779.
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[16]徐杨璐.基于液质联用技术的黄芩物质基础研究[D].承德:承德医学院,2015.
[17]缪晓素,宋国虎,刘容秀,等.不同栽培年限及采收期对黄芩药材有效成分含量的影响研究[J].中国现代中药,2015,17(8):836-839.
[18]安瑜,王旭鹏,赵建军.宁夏六盘山栽培黄芩适宜采收期研究[J].中国实验方剂学杂志,2013,19(13):161-164.
[19]刘素丽,陈建波,周群,等.黄芩采收季节的红外光谱三级鉴别与主成分分析[J].光谱学与光谱分析,2012,32(10):2669-2673.
[2]刘佳,黄晓,余坤,等.云南野生和湖北引种鞘蕊苏有效成分含量与其生态环境的相关性研究[J].中药材,2015,38(6):1141-1143.
[3]胡云飞,吴德玲,徐国兵,等.GC-MS结合化学计量学对5个产地牡丹皮提取物的比较分析[J].中成药,2015,37(9):2003-2007.
[4]叶菊,邱黛玉,曾擎义,等.不同采收期各品种甘草产量和有效成分的比较[J].中成药,2016,38(5):1088-1092.
[5]宋旦哥.基于不同来源的黄芩物质基础与其药效学相关性研究[D].北京:北京中医药大学,2010.
[6]国家药典委员会.中华人民共和国药典:一部[S].2015年版.北京:中国医药科技出版社,2015:301-302.
[7]马芳.红外光谱分析在茯苓资源研究中的应用[D].武汉:武汉轻工大学,2013.
[8]GAJBHIYE NA,MAKASANA J,KUMAR S.Accumulation of three important bioactive compounds in different plant parts of Withania somnifera and its determination by the LC-ESI-MS-MS(MRM)method[J].J Chromatogr Sci,2015,53(10):1749-1756.
[9]刘素丽.黄芩药材的质量控制研究[D].承德:承德医学院,2013.
[10]王鹏,郭庆梅,赵启韬,等.白花丹参不同部位的红外光谱三级鉴定[J].中国实验方剂学杂志,2011,17(9):113-117.
[11]金哲雄,徐胜艳,孙素琴,等.刺五加不同部位的红外光谱分析与鉴定[J].光谱学与光谱分析,2008,28(12):2859-2863.
[12]徐荣,孙素琴,刘友刚,等.红外光谱法用于肉苁蓉属中药鉴别研究[J].光谱学与光谱分析,2010,30(4):897-900.
[13]张春晖,张贵君,孙素琴,等.黄芩及其提取物红外光谱与二维相关光谱的鉴别[J].光谱学与光谱分析,2010,30(7):1774-1779.
[14]LEI Y,ZHOU Q,ZHANG YL,et al.Analysis of crystallized lactose in milk powder by Fourier-transform infrared spectroscopy combined with two-dimensional correlation infrared spectroscopy[J].J Mol Struct,2010,974(1/2/3):88-93.
[15]WU YW,SUN SQ,ZHAO J,et al.Rapid discrimination of extracts of Chinese propolis and poplar buds by FT-IRand 2D IR correlation spectroscopy[J].J Mol Struct,2008.DOI:10.1016/j.molstruc.2007.12.009.
[16]徐杨璐.基于液质联用技术的黄芩物质基础研究[D].承德:承德医学院,2015.
[17]缪晓素,宋国虎,刘容秀,等.不同栽培年限及采收期对黄芩药材有效成分含量的影响研究[J].中国现代中药,2015,17(8):836-839.
[18]安瑜,王旭鹏,赵建军.宁夏六盘山栽培黄芩适宜采收期研究[J].中国实验方剂学杂志,2013,19(13):161-164.
[19]刘素丽,陈建波,周群,等.黄芩采收季节的红外光谱三级鉴别与主成分分析[J].光谱学与光谱分析,2012,32(10):2669-2673.