结肠癌细胞裂解物致敏的树突状细胞-细胞因子诱导杀伤细胞抗自体结肠癌细胞效应研究
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摘要
目的探讨负载结肠癌细胞裂解产物的树突状细胞(DC)-细胞因子诱导的杀伤细胞(CIK)对自体结肠癌细胞的体外杀伤作用。方法选取结肠癌患者切除的肿瘤组织反复冻融,制备结肠癌细胞裂解产物,将结肠癌细胞裂解产物与自体DC共培养。将负载自体结肠癌细胞裂解产物致敏的DC与CIK共培养,形成抗原(Ag)-DC-CIK细胞,检测Ag-DC-CIK细胞的免疫活性以及对自体结肠癌细胞的杀伤效应。结果 Ag-DC-CIK组和DC-CIK组CD3+、CD56+双阳性细胞比例分别为(31.0%±4.1%)和(29.0%±3.4%),显著高于CIK组的(19.0%±2.5%),组间比较,差异有统计学意义(P<0.05)。在同等效靶比条件下,Ag-DC-CIK组对自体肿瘤细胞的杀伤能力强于DC-CIK组和CIK组,组间比较,差异有统计学意义(P<0.05)。结论 Ag-DC-CIK细胞对自体结肠癌细胞的杀伤效应显著强于DC-CIK和CIK细胞。
Objective To evaluate the activity that tumor lysate-pulsed dendritic cell( DC) co-cultured with cytokine-induced killer( CIK) cells in vitro to suppress and kill autologous colon cancer cells. Methods The tumor tissue resected by colon cancer patients was repeatedly freeze-thawed and thawed. The sensitized DC and CIK cells loaded with the cleft product of autogenous colon cancer cells were co-cultured to form Ag-DC-CIK cells,and the immunological activity of Ag-DC-CIK cells and the killing effect on autogenous colon cancer cells were detected. Results The positive CIK cells CD3 + and CD56 + in the Ag-DC-CIK group and the DC-CIK group were( 31. 0% ± 4. 1%) and( 29. 0% ± 3. 4%),which were significantly higher than( 19. 0% ± 2. 5%) in the CIK group,and the difference between the groups was statistically significant( P < 0. 05). Under the condition of the same equivalent target ratio,the killing ability of autologous colon cancer cells in the Ag-DC-CIK group was better than that of the DC-CIK group and the CIK group,and the difference was statistically significant( P < 0. 05). Conclusion Ag-DC-CIK cells are more efficacious than DC-CIK cells and CIK cells on the effects and activity to kill autologous colon cancer cells in vitro.
Objective To evaluate the activity that tumor lysate-pulsed dendritic cell( DC) co-cultured with cytokine-induced killer( CIK) cells in vitro to suppress and kill autologous colon cancer cells. Methods The tumor tissue resected by colon cancer patients was repeatedly freeze-thawed and thawed. The sensitized DC and CIK cells loaded with the cleft product of autogenous colon cancer cells were co-cultured to form Ag-DC-CIK cells,and the immunological activity of Ag-DC-CIK cells and the killing effect on autogenous colon cancer cells were detected. Results The positive CIK cells CD3 + and CD56 + in the Ag-DC-CIK group and the DC-CIK group were( 31. 0% ± 4. 1%) and( 29. 0% ± 3. 4%),which were significantly higher than( 19. 0% ± 2. 5%) in the CIK group,and the difference between the groups was statistically significant( P < 0. 05). Under the condition of the same equivalent target ratio,the killing ability of autologous colon cancer cells in the Ag-DC-CIK group was better than that of the DC-CIK group and the CIK group,and the difference was statistically significant( P < 0. 05). Conclusion Ag-DC-CIK cells are more efficacious than DC-CIK cells and CIK cells on the effects and activity to kill autologous colon cancer cells in vitro.
引文
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[11]Lin M,Liang S,Jiang F,et al.2003-2013,a valuable study:autologous tumor lysate-pulsed dendritic cell immunotherapy with cytokine-induced killer cells improves survival in stageⅣbreast cancer[J].Immunol Lett,2017,183:37-43.
[12]Bohnenkamp HR,Coleman J,Burchell JM,et al.Breast carcinoma cell lysate-pulsed dendritic cells cross-prime MUC1-specific CD8+T cells identified by peptide-MHC-class-I tetramers[J].Cellular Immunology,2004,231(1):112-125.
[13]李坚,李卫党,藏磊,等.树突状细胞与肿瘤细胞融合后对肿瘤发生和生长的抑制作用[J].实用癌症杂志,2000,15(5):453-455.
[2]王子红,鲍云华.奥沙利铂联合替吉奥对晚期结肠癌患者血清相关标志物影响[J].创伤与急危重病医学,2018,6(2):119-121.
[3]陈万青,郑荣寿,曾红梅,等.2011年中国恶性肿瘤发病和死亡分析[J].中国肿瘤,2015,24(1):1-10.
[4]杜娟,刘宝瑞.胃癌免疫治疗新趋势:个体化肽疫苗[J].中国肿瘤生物治疗杂志,2015,22(4):514-518.
[5]Palmer DH,Midgley RS,Mirza N,et al.A phaseⅡstudy of adoptive immunotherapy using dendritic cells pulsed with tumor lysate in patients with hepatocellular carcinoma[J].Hepatology,2009,49(1):124-132.
[6]Matsushita H,Enomoto Y,Kume H,et al.A pilot study of autologous tumor lysate-loaded dendritic cell vaccination combined with sunitinib for metastatic renal cell carcinoma[J].J Immunother Cancer,2014,19(2):30.
[7]方慧云,程伟民,李晓玲,等.CIK细胞治疗晚期大肠癌的临床研究[J].实用癌症杂志,2014,29(8):1008-1012.
[8]卞晋荣.结直肠癌术后化疗联合DC-CIK细胞治疗的效果评价[J].中国肿瘤外科杂志,2013,5:306-308.
[9]Chiang CL,Benencia F,Coukos G.Whole tumor antigen vaccines[J].Semin Immunol,2010,22(3):132-143.
[10]Wang H,Zhou FJ,Wang QJ,et al.Efficacy of autologous renal tumor cell lysate-loaded dendritic cell vaccine in combination with cytokine-induced killer cells on advanced renal cell carcinoma-a report of ten cases[J].Ai Zheng,2006,25(5):625-630.
[11]Lin M,Liang S,Jiang F,et al.2003-2013,a valuable study:autologous tumor lysate-pulsed dendritic cell immunotherapy with cytokine-induced killer cells improves survival in stageⅣbreast cancer[J].Immunol Lett,2017,183:37-43.
[12]Bohnenkamp HR,Coleman J,Burchell JM,et al.Breast carcinoma cell lysate-pulsed dendritic cells cross-prime MUC1-specific CD8+T cells identified by peptide-MHC-class-I tetramers[J].Cellular Immunology,2004,231(1):112-125.
[13]李坚,李卫党,藏磊,等.树突状细胞与肿瘤细胞融合后对肿瘤发生和生长的抑制作用[J].实用癌症杂志,2000,15(5):453-455.