连钱草乙酸乙酯部位化学成分研究
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摘要
目的:研究连钱草的化学成分。方法:采用硅胶柱层析、Sephadex LH-20柱、HPLC分离技术分离纯化连钱草乙酸乙酯部位化学成分,根据化合物的理化性质以及波谱数据鉴定化合物结构。结果:从连钱草乙酸乙酯部位分离并鉴定出15个化合物,分别为:迷迭香酸(1)、迷迭香酸甲酯(2)、丹酚酸A(3)、caffeoylglycolic acid(4)、trichocarpin(5)、isorinic acid(6)、3,4-二咖啡酰基奎尼酸甲酯(7)、(E)-3-((2S,3R)-3-(3,4-dihydroxyphenyl)-2-((E)-3-(3,4-dihydroxyphenyl)acryloyloxy)-2,3-dihydrobenzo[b][1,4]dioxin-6-yl)acrylic acid(8)、eucommin(9)、柯伊利素-7-O-β-D-葡萄糖苷(10)、芹菜素-7-O-β-D-吡喃葡萄糖苷(11)、shimobashiraside C(12)、2-[3-hydroxy-4-(4-hydroxyphenoxy)phenyl]-1-(methoxycarbonyl)ethyl(E)-3-(3,4-dihydroxyphenyl)prop-2-enoate(13)、latifolicinin D(14)、原儿茶酸(15)。结论:其中,化合物3～10、12～14均为首次从该植物中分离得到。
ObJective:To study the chemical constituents from Herba Glechomae.Methods:The columnl chromatography,Sephadex LH-20 and HPLC were used to isolate and purify the compounds of ethyl acetate extract of Herba Glechomae.The chemical structures were identified on basis of physic-chemical properties and spectral data.Results:15 compounds were isolated identified as rosmarinic acid(1),methyl rosmarinate(2),salvianolic acid A(3),caffeoylglycolic acid(4),trichocarpin(5),isorinic acid(6),methyl 3,4-dicaffeoylquinate(7),(E)-3-((2S,3R)-3-(3,4-dihydroxyphenyl)-2-((E)-3-(3,4-dihydroxyphenyl)acryloyloxy)-2,3-dihydrobenzo[b][1,4]dioxin-6-yl)acrylic acid(8),eucommin(9),chrysoeriol-7-O-β-D-glucopyranoside(10),apigenin-7-O-β-D-glucopyranoside(11),shimobashiraside C(12),2-[3-hydroxy-4-(4-hydroxyphenoxy)phenyl]-1-(methoxycarbonyl)ethyl(E)-3-(3,4-dihydroxyphenyl)prop-2-enoate(13),latifolicinin D(14)and protocatechuic acid(15).Conclusion:Compounds 3～10,12～14 are isolated from this plant for the first time.
ObJective:To study the chemical constituents from Herba Glechomae.Methods:The columnl chromatography,Sephadex LH-20 and HPLC were used to isolate and purify the compounds of ethyl acetate extract of Herba Glechomae.The chemical structures were identified on basis of physic-chemical properties and spectral data.Results:15 compounds were isolated identified as rosmarinic acid(1),methyl rosmarinate(2),salvianolic acid A(3),caffeoylglycolic acid(4),trichocarpin(5),isorinic acid(6),methyl 3,4-dicaffeoylquinate(7),(E)-3-((2S,3R)-3-(3,4-dihydroxyphenyl)-2-((E)-3-(3,4-dihydroxyphenyl)acryloyloxy)-2,3-dihydrobenzo[b][1,4]dioxin-6-yl)acrylic acid(8),eucommin(9),chrysoeriol-7-O-β-D-glucopyranoside(10),apigenin-7-O-β-D-glucopyranoside(11),shimobashiraside C(12),2-[3-hydroxy-4-(4-hydroxyphenoxy)phenyl]-1-(methoxycarbonyl)ethyl(E)-3-(3,4-dihydroxyphenyl)prop-2-enoate(13),latifolicinin D(14)and protocatechuic acid(15).Conclusion:Compounds 3～10,12～14 are isolated from this plant for the first time.
引文
[1] 国家药典委员会.中华人民共和国药典[S].一部.北京:中国医药科技出版社,2015:170.
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[11] 普建新,赵静峰,羊晓东,等.藏药心叶兔儿风中黄酮成分的研究[J].中草药,2005,36(6):819-820.
[12] 谢朋飞,邹录惠,邱莉,等.华山矾地上部分的化学成分研究[J].中草药,2014,45(20):2895-2899.
[13] Choudhary MI,Begum A,Abbaskhan A,et al.Two new antioxidant phenylpropanoids from Lindelofia stylosa[J].Chemistry & Biodiversity,2008,5(12):2676-2683.
[14] Siddiqui BS,Perwaiz S,Begum S.Studies on the chemical constituents of the fruits of Cordia latifolia[J].Natural Product Research,2006,20(2):131-137.
[15] 刘轩,张彬锋.白茅根的化学成分研究[J].中国中药杂志,2012,37(15):2296-2300.Chemical Constituents of Ethyl Acetate Extract Part of the Herba Glechomae ………………… DENG Hui-yun,YUAN Ming-ming,DENG Yu,et al.(1103)Chemical Constituents of Caryophylli Flos…………… DAN Chun,JIAO Wei(1108)
[2] 吴小军,宋建晓,赵爱华,等.香青兰酚酸性化学成分的研究[J].天然产物研究与开发,2011,23(3):446-448.
[3] 黄婕,王国才,李桃,等.黄荆的化学成分研究[J].中草药,2013,44(10):1237-1240.
[4] 许枬,曹跃,周翎,等.免疫复方的酚酸类成分研究[J].中国实验方剂学杂志,2011,17(23):85-88.
[5] Stromeier S,Petereit F,Nahrstedt A.Phenolic esters from the rhizomes of Cimicifuga racemosa do not cause proliferation effects in MCF-7 cells[J].Planta Medica,2005,71(6):495-500.
[6] Yang D,Xie H,Jiang Y,et al.Phenolics from strawberry cv.Falandi and their antioxidant and α-glucosidase inhibitory activities[J].Food Chemistry,2016,194:857-863.
[7] 孙照翠,郑庆霞,吴海峰,等.猫须草水溶性化学成分的研究[J].中国药学杂志,2014,49(1):22-25.
[8] 刘静,丁广治,庾石山.毒根斑鸠菊茎皮的化学成分研究[J].中国中药杂志,2010,35(11):1421-1424.
[9] Tsai SF,Lee SS.Neolignans as xanthine oxidase inhibitors from Hyptis rhomboides[J].Phytochemistry,2014,101(9):121-127.
[10] 南泽东,赵明波,姜勇,等.塔中栽培荒漠肉苁蓉中的木脂素类成分[J].中国中药杂志,2015,40(3):463-468.
[11] 普建新,赵静峰,羊晓东,等.藏药心叶兔儿风中黄酮成分的研究[J].中草药,2005,36(6):819-820.
[12] 谢朋飞,邹录惠,邱莉,等.华山矾地上部分的化学成分研究[J].中草药,2014,45(20):2895-2899.
[13] Choudhary MI,Begum A,Abbaskhan A,et al.Two new antioxidant phenylpropanoids from Lindelofia stylosa[J].Chemistry & Biodiversity,2008,5(12):2676-2683.
[14] Siddiqui BS,Perwaiz S,Begum S.Studies on the chemical constituents of the fruits of Cordia latifolia[J].Natural Product Research,2006,20(2):131-137.
[15] 刘轩,张彬锋.白茅根的化学成分研究[J].中国中药杂志,2012,37(15):2296-2300.Chemical Constituents of Ethyl Acetate Extract Part of the Herba Glechomae ………………… DENG Hui-yun,YUAN Ming-ming,DENG Yu,et al.(1103)Chemical Constituents of Caryophylli Flos…………… DAN Chun,JIAO Wei(1108)