响应面法优化刺五加根废弃物多糖提取工艺的研究
|
摘要
目的通过响应面法研究和优化刺五加根废弃物多糖的提取工艺。方法采用苯酚-硫酸法对刺五加根废弃物多糖进行定量分析,在单因素实验的基础上,运用响应面法设计对刺五加根废弃物多糖的提取工艺进行优化。结果响应面优化所得最好条件分别为温度80℃、提取时间1.5 h、液料比21,在最优条件下多糖的平均提取率为10.14%。结论响应面法对刺五加根废弃物多糖的提取条件优化合理,为后续刺五加根废弃物的再资源化开发利用提供一定的理论基础。
Objective To study and optimize the extraction technology of polysaccharides from Acanthopanax senticosus(AS) waste residue by response surface methodology(RSM). Methods Phenol-sulfuric acid method was used to quantitatively analyze polysaccharides from AS waste residue. Based on single factor, the extraction process factors of polysaccharides from AS waste residue were optimized by RSM. Results The best conditions for the response surface optimization were as follows: extraction temperature was 80 ℃, extracting time was 1.5 h, and liquid-material ratio was 21. The extraction rate of polysaccharide was 10.14% under the optimal conditions. Conclusion The extraction conditions of the polysaccharide from AS are optimized by response surface method. This data can provide a theoretical basis for the further exploitation and utilization of the subsequent waste residue of AS.
Objective To study and optimize the extraction technology of polysaccharides from Acanthopanax senticosus(AS) waste residue by response surface methodology(RSM). Methods Phenol-sulfuric acid method was used to quantitatively analyze polysaccharides from AS waste residue. Based on single factor, the extraction process factors of polysaccharides from AS waste residue were optimized by RSM. Results The best conditions for the response surface optimization were as follows: extraction temperature was 80 ℃, extracting time was 1.5 h, and liquid-material ratio was 21. The extraction rate of polysaccharide was 10.14% under the optimal conditions. Conclusion The extraction conditions of the polysaccharide from AS are optimized by response surface method. This data can provide a theoretical basis for the further exploitation and utilization of the subsequent waste residue of AS.
引文
[1]范惠霞,邓志鹏,王福文,等.刺五加注射液在正常大鼠与脑缺血-再灌注损伤疾病大鼠体内药动学比较[J].中成药,2015,37(6):1215-1221.
[2]Meng Q,Pan J,Liu Y,et al.Anti-tumour effects of polysaccharide extracted from Acanthopanax senticosus and cell-mediated immunity[J].Exp Ther Med,2018,15(2):1694-1701.
[3]靳祎,肖锋,武均宜,等.刺五加多糖下调survivin蛋白表达诱导He La细胞凋亡[J].中成药,2016,38(4):902-904.
[4]张静,温暖,刘阳洋,等.响应面法优化蒲公英根多糖超声辅助提取工艺[J].哈尔滨商业大学学报:自然科学版,2018,34(2):209-215.
[5]郝颖,东方,金莉娜,等.响应面法优化黄芪多糖提取工艺[J].哈尔滨商业大学学报:自然科学版,2013,29(1):22-26.
[6]张婷婷,李艺丹,郑凯旋,等.多指标综合加权评分法结合D-最优设计响应面法优选酒川芎的炮制工艺[J].中草药,2018,49(15):3639-3644.
[7]何晨阳,刘明月,时振伟,等.响应面法优化蛇莓花色苷浸提工艺及稳定性研究[J].中草药,2018,49(8):1829-1834.
[8]Braünlich P M,Inngjerdingen K T,Inngjerdingen M,et al.Polysaccharides from the South African medicinal plant Artemisia afra:Structure and activity studies[J].Fitoterapia,2018,124:182-187.
[9]金兰,张海晶,陈大忠.星点设计-响应面法优化北五味子多糖提取工艺[J].哈尔滨商业大学学报:自然科学版,2013,29(6):649-652.
[10]张娜,毛迪,安柏松,等.刺五加多糖对免疫性肝损伤BALB/c小鼠IL-2、IL-4、INF-γ细胞因子及m RNA表达的影响[J].药物评价研究,2018,41(4):557-561.
[11]刘喜舞,郑礼胜,谢霞,等.细柱五加叶多糖提取分离纯化及保湿性能研究[J].中草药,2015,46(8):1167-1173.
[12]张英楠.刺五加多糖对鸡的免疫调节作用及其机理研究[D].长春:吉林农业大学,2016.
[13]Udoetok I A,Wilson L D,Headley J V.Self-assembled and cross-linked animal and plant-based polysaccharides:Chitosan-cellulose composites and their anion uptake properties[J].ACS Appl Mater Interf,2016,8(48):33197-33209.
[14]劳凤云,张志斐,刑朝斌.高效液相色谱法测定不同产地刺五加根、茎中绿原酸的含量[J].中国煤炭工业医学杂志,2013,16(6):930-931.
[15]何晨阳,刘明月,时振伟,等.响应面法优化蛇莓花色苷浸提工艺及稳定性研究[J].中草药,2018,49(8):1829-1834.
[16]孟庆龙.长白山刺五加多糖开发的药理基础研究[D].长春:吉林农业大学,2018.
[17]俞娟,刘劲松,王刚,等.响应面法优化马兰多糖提取工艺研究[J].中成药,2015,37(1):222-225.
[2]Meng Q,Pan J,Liu Y,et al.Anti-tumour effects of polysaccharide extracted from Acanthopanax senticosus and cell-mediated immunity[J].Exp Ther Med,2018,15(2):1694-1701.
[3]靳祎,肖锋,武均宜,等.刺五加多糖下调survivin蛋白表达诱导He La细胞凋亡[J].中成药,2016,38(4):902-904.
[4]张静,温暖,刘阳洋,等.响应面法优化蒲公英根多糖超声辅助提取工艺[J].哈尔滨商业大学学报:自然科学版,2018,34(2):209-215.
[5]郝颖,东方,金莉娜,等.响应面法优化黄芪多糖提取工艺[J].哈尔滨商业大学学报:自然科学版,2013,29(1):22-26.
[6]张婷婷,李艺丹,郑凯旋,等.多指标综合加权评分法结合D-最优设计响应面法优选酒川芎的炮制工艺[J].中草药,2018,49(15):3639-3644.
[7]何晨阳,刘明月,时振伟,等.响应面法优化蛇莓花色苷浸提工艺及稳定性研究[J].中草药,2018,49(8):1829-1834.
[8]Braünlich P M,Inngjerdingen K T,Inngjerdingen M,et al.Polysaccharides from the South African medicinal plant Artemisia afra:Structure and activity studies[J].Fitoterapia,2018,124:182-187.
[9]金兰,张海晶,陈大忠.星点设计-响应面法优化北五味子多糖提取工艺[J].哈尔滨商业大学学报:自然科学版,2013,29(6):649-652.
[10]张娜,毛迪,安柏松,等.刺五加多糖对免疫性肝损伤BALB/c小鼠IL-2、IL-4、INF-γ细胞因子及m RNA表达的影响[J].药物评价研究,2018,41(4):557-561.
[11]刘喜舞,郑礼胜,谢霞,等.细柱五加叶多糖提取分离纯化及保湿性能研究[J].中草药,2015,46(8):1167-1173.
[12]张英楠.刺五加多糖对鸡的免疫调节作用及其机理研究[D].长春:吉林农业大学,2016.
[13]Udoetok I A,Wilson L D,Headley J V.Self-assembled and cross-linked animal and plant-based polysaccharides:Chitosan-cellulose composites and their anion uptake properties[J].ACS Appl Mater Interf,2016,8(48):33197-33209.
[14]劳凤云,张志斐,刑朝斌.高效液相色谱法测定不同产地刺五加根、茎中绿原酸的含量[J].中国煤炭工业医学杂志,2013,16(6):930-931.
[15]何晨阳,刘明月,时振伟,等.响应面法优化蛇莓花色苷浸提工艺及稳定性研究[J].中草药,2018,49(8):1829-1834.
[16]孟庆龙.长白山刺五加多糖开发的药理基础研究[D].长春:吉林农业大学,2018.
[17]俞娟,刘劲松,王刚,等.响应面法优化马兰多糖提取工艺研究[J].中成药,2015,37(1):222-225.