基于抗Aβ1-42淀粉样蛋白活性的不同产地姜黄质量评价研究
|
摘要
目的:以抗Aβ1-42淀粉样蛋白功效指标为核心,结合化学成分和毒性效应对不同产地的姜黄进行综合质量评价。方法:以全国范围内7个省份41个样品采集点收集药材,参考2015版《中国药典》规定测定姜黄的指标成分(姜黄素和挥发油)含量;开展抗Aβ1-42淀粉样蛋白功效试验:表面等离子共振技术分析样品与Aβ1-42及其纤维体fAβ1-42的结合力、fAβ1-42致PC12细胞损伤模型分析样品对损伤细胞的保护作用;以正常生长的PC12细胞检测药物毒性;以功效指标为核心分别对样品成分指标和毒性效应做相关性分析并对不同产地的姜黄抗淀粉样蛋白活性进行综合质量评价。结果:姜黄抗Aβ1-42淀粉蛋白活性功效指标与化学成分姜黄素、挥发油含量成正相关,与毒性效应不相关;各产区中,四川产姜黄抗Aβ1-42淀粉样蛋白活性最强、功效成分姜黄素与挥发油含量最高、毒性效应与其余产地无差异,可认为四川产姜黄质量最佳。结论:以功效为核心的中药质量评价模式可明确中药材在具体功效上的质量优劣,相对以传统感官及化学评价,中药整体品质评价更能体现复杂体系整体控制的设计思想。
Objective: To evaluate the quality of Curcuma longae from different areas by using the efficacy index of anti-Aβ1-42 amyloid protein,combining with its chemical composition and toxicity effect. Methods: Medicinal materials were collected from 41 sampling sites in seven provinces,and the contents of curcumin and volatile oil of Curcuma longae were determined according to Chinese Pharmacopoeia( 2015 edition). The efficacy test of anti-Aβ1-42 amyloid protein was performed,Surface Plasmon Resonanc( SPR) technique was used to analyze the binding of samples with Aβ1-42 and its fiber fA β1-42. The protective effects of samples on PC12 cell injury model induced by fA β1-42 were analyzed. The drug toxicity was detected by normal-growing PC12 cells,and the correlation between chemical composition and toxic effects of samples was analyzed by efficacy index. The anti-amyloid activity of Curcuma longae from different areas was evaluated comprehensively. Result: Efficacy index of anti-Aβ1-42 amyloid protein of Curcuma longae had positive correlation to the content of Curcumin and volatile oil,but was not related to the toxicity effect. Curcuma longae from Sichuan showed the best quality. Conclusion: The quality evaluation of traditional Chinese medicines focused on efficacy can determine the quality of the specific efficacy of Chinese medicines. Compared with the traditional sensory and chemical evaluation,the overall quality evaluation of traditional Chinese medicines can reflect the overall control of complex systems better.
Objective: To evaluate the quality of Curcuma longae from different areas by using the efficacy index of anti-Aβ1-42 amyloid protein,combining with its chemical composition and toxicity effect. Methods: Medicinal materials were collected from 41 sampling sites in seven provinces,and the contents of curcumin and volatile oil of Curcuma longae were determined according to Chinese Pharmacopoeia( 2015 edition). The efficacy test of anti-Aβ1-42 amyloid protein was performed,Surface Plasmon Resonanc( SPR) technique was used to analyze the binding of samples with Aβ1-42 and its fiber fA β1-42. The protective effects of samples on PC12 cell injury model induced by fA β1-42 were analyzed. The drug toxicity was detected by normal-growing PC12 cells,and the correlation between chemical composition and toxic effects of samples was analyzed by efficacy index. The anti-amyloid activity of Curcuma longae from different areas was evaluated comprehensively. Result: Efficacy index of anti-Aβ1-42 amyloid protein of Curcuma longae had positive correlation to the content of Curcumin and volatile oil,but was not related to the toxicity effect. Curcuma longae from Sichuan showed the best quality. Conclusion: The quality evaluation of traditional Chinese medicines focused on efficacy can determine the quality of the specific efficacy of Chinese medicines. Compared with the traditional sensory and chemical evaluation,the overall quality evaluation of traditional Chinese medicines can reflect the overall control of complex systems better.
引文
[1]国家药典委员会编.中华人民共和药典(一部). 北京:中国医药科技出版社,2015∶264.
[2]李青苗,杨文钰,唐雪梅,等.姜黄素类化合物在不同品系姜黄根茎内积累规律研究 .中国中药杂志,2014,39(11)∶2000～2004.
[3]曹柳,赵军宁,王晓宇,等.不同加工方法对不同产地姜黄、郁金药材中姜黄素类成分含量的影响 .中国实验方剂学杂志,2016,(4)∶50～56.
[4]周丽娟,赵军宁,徐海波,等.不同商品规格、等级姜黄提取物抗氧化作用比较研究 .中药药理与临床,2016,(1)∶110～112.
[5]唐宜轩,马旻新,朱宁,等.不同产地姜黄中挥发油及姜黄色素的含量分析 . 中国实验方剂学杂志,2016,(5)∶30～35.
[6]廖涛,王飞,张占军,等.姜黄素对痴呆模型小鼠学习记忆能力的改善作用及对细胞凋亡的影响 .中国药理学通报,2009,25(10)∶1359～1363.
[7]王运良,尹红蕾,娄季宇,等.姜黄素对β淀粉样蛋白诱导的老年性痴呆大鼠小胶质细胞活化的影响 .中华临床医师杂志(电子版),2011,5(19)∶5578～5582.
[8]韩玉生,侯志涛,纪显玥,等.姜黄素调控β-分泌酶基因表达抑制老年性痴呆大鼠Aβ蛋白生成的机制 .中医学报,2015,(4)∶536～538.
[9]赵军宁,鄢良春,宋军.建立以"功效"为核心的新型中药质量评价模式 .中药药理与临床,2010,26(5)∶158～161.
[10]陈雪,孙婧霞,刘源劼,等.β-淀粉样蛋白1-42寡聚体诱导的阿尔茨海默病大鼠海马的BCl-2表达和caspase-3活性变化 .解剖学杂志,2014,37(2)∶181～184.
[11]Garcez M L, Mina F, Bellettini-Santos T, et al. Minocycline reduces inflammatory parameters in the brain structures and serum and reverses memory impairment caused by the administration of amyloid β (1-42) in mice . Progress in Neuro-Psychopharmacology and Biological Psychiatry, 2017, 77∶23～31.
[12]Li X, Zhao X, Xu X, et al. Schisantherin A recovers Aβ-induced neurodegeneration with cognitive decline in mice. . Physiology & Behavior, 2014, 132(132)∶10～16.
[13]Eun Ju Jeong, Hee Kyoung Lee, Ki Yong Lee, et al. The effects of lignan-riched extract of Shisandra chinensis, on amyloid-β-induced cognitive impairment and neurotoxicity in the cortex and hippocampus of mouse . Journal of Ethnopharmacology, 2013, 146(1)∶347～354.
[14]曾瑾,罗霞,郑林用,等.灵芝发酵液诱导PC12细胞分化及β淀粉样肽细胞毒性的保护作用 .中药药理与临床,2009(5)∶82～85.
[15]Esposito G, De F D, Maiuri M C, et al. Cannabidiol inhibits inducible nitric oxide synthase protein expression and nitric oxide production in beta-amyloid stimulated PC12 neurons through p38 MAP kinase and NF-kappaB involvement. Neuroscience Letters, 2006, 399(1)∶91～95.
[16]Wang C, Ma H, Zhang S, et al. Safflor yellow B suppresses pheochromocytoma cell (PC12) injury induced by oxidative stress via antioxidant system and BCl-2 /Bax pathway. . Naunyn Schmiedebergs Arch Pharmacol,2009,380(2)∶135～142.
[17]Cheng S B, Wu L C, Hsieh Y C, et al. Supercritical carbon dioxide extraction of aromatic turmerone from Curcuma longa Linn. induces apoptosis through reactive oxygen species-triggered intrinsic and extrinsic pathways in human hepatocellular carcinoma HepG2 cells . Journal of Agricultural & Food Chemistry, 2012, 60(38)∶9620～9630.
[18]Su J Y, Tan L R, Lai P, et al. Experimental study on anti-inflammatory activity of a TCM recipe consisting of the supercritical fluid CO2, extract of Chrysanthemum indicum, Patchouli Oil and Zedoary Turmeric Oil in vivo . Journal of Ethnopharmacology, 2012, 141(2)∶608～614.
[19]刘不悔,顾一煌,涂玥,等. 衰老的分子调控机制及中药的干预作用 .中国中药杂志,2017,42(16)∶3065～3071.
[20]王颖,郭兰萍,黄璐琦,等. 姜黄、莪术、郁金的化学成分与药理作用研究进展 .中国药房,2013,24(35)∶3338～3342.
[21]薛月萃,周英,康颖倩,等.姜黄挥发油的研究进展 .华西药学杂志,2016,31(02)∶212～215.
[22]张姗,曾常茜.姜黄提取物与阿尔茨海默病研究进展 .中国合理用药探索,2017,14(5)∶61～63.
[23]杨融辉,张宁.姜黄素对阿尔茨海默病作用机制的研究进展 .东南大学学报(医学版),2015(1)∶152～155.
[24]狄建彬,顾振纶,赵笑东,等.姜黄素的抗氧化和抗炎作用研究进展 .中草药, 2010, 41(5)∶854～857.
[25]唐小卿,聂亚雄,冯鉴强,等.姜黄素对H2O2损伤PC12细胞的保护作用 .中国药理学通报,2004,20(6)∶672～676.
[26]Mhillaj E, Morgese M G, Tucci P, et al. Celecoxib Prevents Cognitive Impairment and Neuroinflammation in Soluble Amyloid β-treated rats. Neuroscience, 2018,372∶ 58～73.
[27]Cao C, Li Y, Liu H, et al. The potential therapeutic effects of THC on Alzheimer's disease. Journal of Alzheimers Disease Jad, 2014, 42(3)∶973.
[28]Omtri R S, Thompson K, Tang X, et al. Differential Effects of Alzheimer's Disease Aβ40 and 42 on Endocytosis and Intraneuronal Trafficking. Neuroscience, 2018, 373∶159～168.
[29]Murray M M, Bernstein S L, Nyugen V, et al. Amyloid beta protein: Abeta40 inhibits Abeta42 oligomerization. Journal of the American Chemical Society, 2009, 131(18)∶6316～6317.
[30]Zou K, Kim D, Kakio A, et al. Amyloid beta-protein (Abeta)1-40 protects neurons from damage induced by Abeta1-42 in culture and in rat brain. Journal of Neurochemistry, 2003, 87(3)∶609～619.
[31]Craig Osborne, Ewan West, William Nolan,等. Glimepiride protects neurons against amyloid-β-induced synapse damage. Neuropharmacology, 2016, 101∶225～236.
[2]李青苗,杨文钰,唐雪梅,等.姜黄素类化合物在不同品系姜黄根茎内积累规律研究 .中国中药杂志,2014,39(11)∶2000～2004.
[3]曹柳,赵军宁,王晓宇,等.不同加工方法对不同产地姜黄、郁金药材中姜黄素类成分含量的影响 .中国实验方剂学杂志,2016,(4)∶50～56.
[4]周丽娟,赵军宁,徐海波,等.不同商品规格、等级姜黄提取物抗氧化作用比较研究 .中药药理与临床,2016,(1)∶110～112.
[5]唐宜轩,马旻新,朱宁,等.不同产地姜黄中挥发油及姜黄色素的含量分析 . 中国实验方剂学杂志,2016,(5)∶30～35.
[6]廖涛,王飞,张占军,等.姜黄素对痴呆模型小鼠学习记忆能力的改善作用及对细胞凋亡的影响 .中国药理学通报,2009,25(10)∶1359～1363.
[7]王运良,尹红蕾,娄季宇,等.姜黄素对β淀粉样蛋白诱导的老年性痴呆大鼠小胶质细胞活化的影响 .中华临床医师杂志(电子版),2011,5(19)∶5578～5582.
[8]韩玉生,侯志涛,纪显玥,等.姜黄素调控β-分泌酶基因表达抑制老年性痴呆大鼠Aβ蛋白生成的机制 .中医学报,2015,(4)∶536～538.
[9]赵军宁,鄢良春,宋军.建立以"功效"为核心的新型中药质量评价模式 .中药药理与临床,2010,26(5)∶158～161.
[10]陈雪,孙婧霞,刘源劼,等.β-淀粉样蛋白1-42寡聚体诱导的阿尔茨海默病大鼠海马的BCl-2表达和caspase-3活性变化 .解剖学杂志,2014,37(2)∶181～184.
[11]Garcez M L, Mina F, Bellettini-Santos T, et al. Minocycline reduces inflammatory parameters in the brain structures and serum and reverses memory impairment caused by the administration of amyloid β (1-42) in mice . Progress in Neuro-Psychopharmacology and Biological Psychiatry, 2017, 77∶23～31.
[12]Li X, Zhao X, Xu X, et al. Schisantherin A recovers Aβ-induced neurodegeneration with cognitive decline in mice. . Physiology & Behavior, 2014, 132(132)∶10～16.
[13]Eun Ju Jeong, Hee Kyoung Lee, Ki Yong Lee, et al. The effects of lignan-riched extract of Shisandra chinensis, on amyloid-β-induced cognitive impairment and neurotoxicity in the cortex and hippocampus of mouse . Journal of Ethnopharmacology, 2013, 146(1)∶347～354.
[14]曾瑾,罗霞,郑林用,等.灵芝发酵液诱导PC12细胞分化及β淀粉样肽细胞毒性的保护作用 .中药药理与临床,2009(5)∶82～85.
[15]Esposito G, De F D, Maiuri M C, et al. Cannabidiol inhibits inducible nitric oxide synthase protein expression and nitric oxide production in beta-amyloid stimulated PC12 neurons through p38 MAP kinase and NF-kappaB involvement. Neuroscience Letters, 2006, 399(1)∶91～95.
[16]Wang C, Ma H, Zhang S, et al. Safflor yellow B suppresses pheochromocytoma cell (PC12) injury induced by oxidative stress via antioxidant system and BCl-2 /Bax pathway. . Naunyn Schmiedebergs Arch Pharmacol,2009,380(2)∶135～142.
[17]Cheng S B, Wu L C, Hsieh Y C, et al. Supercritical carbon dioxide extraction of aromatic turmerone from Curcuma longa Linn. induces apoptosis through reactive oxygen species-triggered intrinsic and extrinsic pathways in human hepatocellular carcinoma HepG2 cells . Journal of Agricultural & Food Chemistry, 2012, 60(38)∶9620～9630.
[18]Su J Y, Tan L R, Lai P, et al. Experimental study on anti-inflammatory activity of a TCM recipe consisting of the supercritical fluid CO2, extract of Chrysanthemum indicum, Patchouli Oil and Zedoary Turmeric Oil in vivo . Journal of Ethnopharmacology, 2012, 141(2)∶608～614.
[19]刘不悔,顾一煌,涂玥,等. 衰老的分子调控机制及中药的干预作用 .中国中药杂志,2017,42(16)∶3065～3071.
[20]王颖,郭兰萍,黄璐琦,等. 姜黄、莪术、郁金的化学成分与药理作用研究进展 .中国药房,2013,24(35)∶3338～3342.
[21]薛月萃,周英,康颖倩,等.姜黄挥发油的研究进展 .华西药学杂志,2016,31(02)∶212～215.
[22]张姗,曾常茜.姜黄提取物与阿尔茨海默病研究进展 .中国合理用药探索,2017,14(5)∶61～63.
[23]杨融辉,张宁.姜黄素对阿尔茨海默病作用机制的研究进展 .东南大学学报(医学版),2015(1)∶152～155.
[24]狄建彬,顾振纶,赵笑东,等.姜黄素的抗氧化和抗炎作用研究进展 .中草药, 2010, 41(5)∶854～857.
[25]唐小卿,聂亚雄,冯鉴强,等.姜黄素对H2O2损伤PC12细胞的保护作用 .中国药理学通报,2004,20(6)∶672～676.
[26]Mhillaj E, Morgese M G, Tucci P, et al. Celecoxib Prevents Cognitive Impairment and Neuroinflammation in Soluble Amyloid β-treated rats. Neuroscience, 2018,372∶ 58～73.
[27]Cao C, Li Y, Liu H, et al. The potential therapeutic effects of THC on Alzheimer's disease. Journal of Alzheimers Disease Jad, 2014, 42(3)∶973.
[28]Omtri R S, Thompson K, Tang X, et al. Differential Effects of Alzheimer's Disease Aβ40 and 42 on Endocytosis and Intraneuronal Trafficking. Neuroscience, 2018, 373∶159～168.
[29]Murray M M, Bernstein S L, Nyugen V, et al. Amyloid beta protein: Abeta40 inhibits Abeta42 oligomerization. Journal of the American Chemical Society, 2009, 131(18)∶6316～6317.
[30]Zou K, Kim D, Kakio A, et al. Amyloid beta-protein (Abeta)1-40 protects neurons from damage induced by Abeta1-42 in culture and in rat brain. Journal of Neurochemistry, 2003, 87(3)∶609～619.
[31]Craig Osborne, Ewan West, William Nolan,等. Glimepiride protects neurons against amyloid-β-induced synapse damage. Neuropharmacology, 2016, 101∶225～236.