摘要
目的:以抗Aβ1-42淀粉样蛋白功效指标为核心,结合化学成分和毒性效应对不同产地的姜黄进行综合质量评价。方法:以全国范围内7个省份41个样品采集点收集药材,参考2015版《中国药典》规定测定姜黄的指标成分(姜黄素和挥发油)含量;开展抗Aβ1-42淀粉样蛋白功效试验:表面等离子共振技术分析样品与Aβ1-42及其纤维体fAβ1-42的结合力、fAβ1-42致PC12细胞损伤模型分析样品对损伤细胞的保护作用;以正常生长的PC12细胞检测药物毒性;以功效指标为核心分别对样品成分指标和毒性效应做相关性分析并对不同产地的姜黄抗淀粉样蛋白活性进行综合质量评价。结果:姜黄抗Aβ1-42淀粉蛋白活性功效指标与化学成分姜黄素、挥发油含量成正相关,与毒性效应不相关;各产区中,四川产姜黄抗Aβ1-42淀粉样蛋白活性最强、功效成分姜黄素与挥发油含量最高、毒性效应与其余产地无差异,可认为四川产姜黄质量最佳。结论:以功效为核心的中药质量评价模式可明确中药材在具体功效上的质量优劣,相对以传统感官及化学评价,中药整体品质评价更能体现复杂体系整体控制的设计思想。
Objective: To evaluate the quality of Curcuma longae from different areas by using the efficacy index of anti-Aβ1-42 amyloid protein,combining with its chemical composition and toxicity effect. Methods: Medicinal materials were collected from 41 sampling sites in seven provinces,and the contents of curcumin and volatile oil of Curcuma longae were determined according to Chinese Pharmacopoeia( 2015 edition). The efficacy test of anti-Aβ1-42 amyloid protein was performed,Surface Plasmon Resonanc( SPR) technique was used to analyze the binding of samples with Aβ1-42 and its fiber fA β1-42. The protective effects of samples on PC12 cell injury model induced by fA β1-42 were analyzed. The drug toxicity was detected by normal-growing PC12 cells,and the correlation between chemical composition and toxic effects of samples was analyzed by efficacy index. The anti-amyloid activity of Curcuma longae from different areas was evaluated comprehensively. Result: Efficacy index of anti-Aβ1-42 amyloid protein of Curcuma longae had positive correlation to the content of Curcumin and volatile oil,but was not related to the toxicity effect. Curcuma longae from Sichuan showed the best quality. Conclusion: The quality evaluation of traditional Chinese medicines focused on efficacy can determine the quality of the specific efficacy of Chinese medicines. Compared with the traditional sensory and chemical evaluation,the overall quality evaluation of traditional Chinese medicines can reflect the overall control of complex systems better.
引文
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