IL-33及其受体sST2在SLE患儿血清中的表达及意义
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摘要
目的探讨系统性红斑狼疮(SLE)患儿血清白细胞介素(IL)33及其受体可溶性致癌抑制因子2(sST2)的表达及意义。方法采用酶联免疫吸附试验(ELISA)检测32例SLE患儿(SLE组)和50名体检健康儿童(正常对照组)的血清IL-33、sST2水平,并分析二者与疾病活动度[系统性红斑狼疮疾病活动度指数2000(SLEDAI-2000)、抗双链DNA(抗dsDNA)抗体]的关系。结果 SLE组血清IL-33、sST2水平均明显高于正常对照组(P<0.01)。Spearman相关分析结果显示,SLE患儿血清IL-33、sST2水平与SLEDAI-2000评分均呈正相关(r值分别为0.399、0.502,P值分别为0.024、0.003),与抗dsDNA抗体滴度也均呈正相关(r值分别为0.395、0.529,P值分别为0.025、0.002)。结论 IL-33、s ST2可能在儿童SLE的发病过程中起重要作用。
Objective To investigate the expressions and roles of serum interleukin 33(IL-33)and soluble suppression of tumorigenicity 2(sST2) in children with systemic lupus erythematosus(SLE). Methods Serum levels of IL-33 and sST2 in 32 children with SLE(SLE group) and 50 healthy children(healthy control group)were determined by enzyme-linked immunosorbent assay(ELISA). The correlations between IL-33,sST2 and systemic lupus erythematosus disease activity index 2000(SLEDAI-2000),anti-double-stranded DNA(antidsDNA) antibody were evaluated. Results Serum levels of IL-33 and sST2 were higher in SLE group than those in healthy control group(P<0.01). Spearman correlation analysis showed that serum levels of IL-33 and sST2 in children with SLE were positively correlated with SLEDAI-2000(r=0.399 and 0.502,P=0.024 and 0.003,respectively). Serum levels of IL-33 and sST2 in children with SLE were positively correlated with anti-dsDNA antibody(r=0.395 and 0.529,P=0.025 and 0.002,respectively). Conclusions Serum IL-33 and sST2 may play roles in the pathogenesis of SLE among children.
Objective To investigate the expressions and roles of serum interleukin 33(IL-33)and soluble suppression of tumorigenicity 2(sST2) in children with systemic lupus erythematosus(SLE). Methods Serum levels of IL-33 and sST2 in 32 children with SLE(SLE group) and 50 healthy children(healthy control group)were determined by enzyme-linked immunosorbent assay(ELISA). The correlations between IL-33,sST2 and systemic lupus erythematosus disease activity index 2000(SLEDAI-2000),anti-double-stranded DNA(antidsDNA) antibody were evaluated. Results Serum levels of IL-33 and sST2 were higher in SLE group than those in healthy control group(P<0.01). Spearman correlation analysis showed that serum levels of IL-33 and sST2 in children with SLE were positively correlated with SLEDAI-2000(r=0.399 and 0.502,P=0.024 and 0.003,respectively). Serum levels of IL-33 and sST2 in children with SLE were positively correlated with anti-dsDNA antibody(r=0.395 and 0.529,P=0.025 and 0.002,respectively). Conclusions Serum IL-33 and sST2 may play roles in the pathogenesis of SLE among children.
引文
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[11]AKCAY A,NGUYEN Q,HE Z,et al.IL-33 exacerbates acute kidney injury[J].J Am Soc Nephrol,2011,22(11):2057-2067.
[12]YANG Z,LIANG Y,XI W,et al.Association of increased serum IL-33 levels with clinical and laboratory characteristics of systemic lupus erythematosus in Chinese population[J].Clin Exp Med,2011,11(2):75-80.
[2]李池慧,郑冰,俞翀曌.895例系统性红斑狼疮患者自身抗体检测结果的分析[J].检验医学,2018,33(5):463-465.
[3]AGGARWAL A,SRIVASTAVA P.Childhood onest systemic lupus erythematosus:how is it different from adult SLE?[J].Int J Rheum Dis,2015,8(2):182-191.
[4]胡坚.儿童系统性红斑狼疮去激素化治疗的思考[J].中华实用儿科临床杂志,2016,31(21):1608-1611.
[5]PETRI M,ORBAI A M,ALARCóN G S,et al.Derivation and validation of the systemic lupus international collaborating clinics classification criteria for systemic lupus erythematosus[J].Arthritis Rheum,2012,64(8):2677-2686.
[6]MOK M Y,HUANG F P,IP W K,et al.Serum levels of IL-33 and soluble ST2 and their association with disease activity in systemic lupus erythematosus[J].Rheumatology(Oxford),2010,49(3):520-527.
[7]BOUFFI C,ROCHMAN M,ZUST C B,et al.IL-33 markedly activates murine eosinophils by an NF-κB-dependent mechanism differentially dependent upon an IL-4-driven autoinflammatory loop[J].J Immunol,2013,191(8):4317-4325.
[8]LIVINGSTON B,BONNER A,POPE J.Differences in clinical manifestations between childhood-onset lupus and adult-onset lupus:a meta-analysis[J].Lupus,2011,20(15):1345-1355.
[9]赵银霞,朱红娟,梁丽俊.儿童系统性红斑狼疮的临床特点[J].宁夏医学杂志,2012,34(12):1251-1252.
[10]LI P,LIN W,ZHENG X.IL-33 neutralization suppresses lupus disease in lupus-prone mice[J].Inflammation,2014,37(3):824-832.
[11]AKCAY A,NGUYEN Q,HE Z,et al.IL-33 exacerbates acute kidney injury[J].J Am Soc Nephrol,2011,22(11):2057-2067.
[12]YANG Z,LIANG Y,XI W,et al.Association of increased serum IL-33 levels with clinical and laboratory characteristics of systemic lupus erythematosus in Chinese population[J].Clin Exp Med,2011,11(2):75-80.