陕西区域茯砖茶中“金花”含量分析
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摘要
为了研究陕西省区域内茯砖茶中"金花"含量分布情况,采用国标方法 GB 4789.15-2016的第一法对FZC-1～FZC-50共50份茯砖茶中"金花"含量进行检测。结果表明,不同茶叶品种基质上的冠突散囊菌在同一培养基上的生长速率不同,增殖方式为有性繁殖和无性繁殖,菌落呈现黄色、暗黄色、褐色或淡黄色。"金花"含量最高的为FZC-35,350×10~4CFU/g;含量最低的为FZC-2和FZC-4,均为20×10~4CFU/g。有48份茯砖茶"金花"含量均符合GB/T9833.3-2013《紧压茶第三部分:茯砖茶》中对冠突散囊菌含量的最低要求(20×10~4CFU/g); 44份茯砖茶"金花"含量符合DBS61/0006-2014《食品安全地方标准泾阳茯砖茶》中对冠突散囊菌含量的最低要求(30×10~4CFU/g)。研究表明,陕西省区域内不同品种茯砖茶中"金花"含量差异较大。
Objective: To analyze the content distribution of"Golden Flower"in Fuzhuan Tea in Shaanxi Province. Methods:According to the method of GB 5413.15,the content of"Golden Flower"of fifty samples FZC-1～ FZC-50 were tested.Results:The growth rates of different strains on the same medium were different,the reproductive made of the strains was sexual reproduction and asexual reproduction,the colonies were yellow,dark yellow,brown or yellowish. The highest content of"Golden Flower"was FZC-35,350 × 10~4 CFU/g. The lowest content was FZC-2 and FZC-4,which was 20 × 10~4 CFU/g.48 samples met the standard GB/T 9833.3-2013 minimum requirement (20 × 10~4 CFU/g).44 samples met the standard DBS61/0006-2014 minimum requirement (30 × 10~4 CFU/g). The results showed that there were great differences in the content of"Golden Flower"among different brick tea in Shaanxi area.
Objective: To analyze the content distribution of"Golden Flower"in Fuzhuan Tea in Shaanxi Province. Methods:According to the method of GB 5413.15,the content of"Golden Flower"of fifty samples FZC-1～ FZC-50 were tested.Results:The growth rates of different strains on the same medium were different,the reproductive made of the strains was sexual reproduction and asexual reproduction,the colonies were yellow,dark yellow,brown or yellowish. The highest content of"Golden Flower"was FZC-35,350 × 10~4 CFU/g. The lowest content was FZC-2 and FZC-4,which was 20 × 10~4 CFU/g.48 samples met the standard GB/T 9833.3-2013 minimum requirement (20 × 10~4 CFU/g).44 samples met the standard DBS61/0006-2014 minimum requirement (30 × 10~4 CFU/g). The results showed that there were great differences in the content of"Golden Flower"among different brick tea in Shaanxi area.
引文
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[17]陈然,郝彬秀,田海霞,等.六堡茶真菌分布浅析及金花菌筛选鉴定[J].食品科技,2016,41(4):19-23.
[18]陕西省卫生和计划生育委员会.DBS61/0006-2014,食品安全地方标准泾阳茯砖茶[S].2014.
[19]彭晓赟,章卫民,刘淑云,等.湖南地区茯砖茶中金花菌的分离鉴定[J].菌物研究,2011,9(3):157-161.
[20]陈桂梅,黄亚亚,梁艳,等.陕西省茯砖茶“金花”菌的鉴定分析[J].湖北农业科学,2013,55(2):345-348.
[21]谭玉梅,王亚萍,葛永怡,等.贵州地区茯砖茶“金花菌”的分离和分子鉴定[J].菌物学报,2017,36(2):154-163.
[2]齐祖同,孙曾美.茯砖茶中优势菌种的鉴定[J].真菌学报,1990,9(3):176-179.
[3]黄彦,石瑞,苏二正.冠突散囊菌的研究与应用进展[J].生物加工过程,2017,15(1):49-56.
[4]蔡正安,刘素纯,刘仲华,等.茯砖茶中冠突散囊菌纤维素酶的酶学性质研究[J].茶叶科学,2010,30(1):57-62.
[5]刘丽萍,唐雨薇,王若娴,等.茯茶冠突散囊菌发酵生产吲哚生物碱的研究[J].茶叶科学,2017,37(5):503-512.
[6]刘闫,胡茂丰,刘素纯.冠突散囊菌产阿魏酸酯酶及其发酵麸皮和黑毛茶形成阿魏酸的研究[J].农产品加工,2015,4:1-4.
[7]李玉婷,吕嘉枥,李良凤,等.冠突散囊菌固态发酵产消化酶活力的研究[J].中国酿造,2012,31(10):93-95.
[8]吕嘉枥,郑欣欣,马亚宁.冠突散囊菌液体发酵产降脂类物质的初步研究[J].陕西科技大学学报,2014,32(6):115-118.
[9]覃金球,罗美玲,张旋,林发全.冠突散囊菌医药价值研究进展[J/OL].食品工业科技:1-8[2018-12-03].http://kns.cnki.net/kcms/detail/11.1759.TS.20180717.1545.016.html.
[10]吴凯为,杨立娜,朱力杰,等.冠突散囊菌在发酵茶中应用的研究进展[J].食品研究与开发,2018,39(1):189-193.
[11]徐永立,赵云林,曾红远,等.不同类型冠突散囊菌对茯砖散茶主要化学成分的影响[J].中国农学通报,2012,29(18):200-205.
[12]李适,龚雪,刘仲华,等.冠突散囊菌对茶叶品质成分的影响研究[J].菌物学报,2014,33(3):713-718.
[13]黄浩,刘仲华,黄建安,等.“发花”散茶中“金花”菌的分离鉴定[J].茶叶科学,2010,30(5):350-354.
[14]胡治远,赵云林,刘素纯,等.不同品种茯砖茶中优势微生物的分离鉴定[J].江西农业学报,2011,23(12):60-64.
[15]中国国家标准化管理委员会.GB/T 9833.3-2013,紧压茶第三部分:茯砖茶[S].2010.
[16]李宏梁,辛乐,舒静,等.茯砖茶中冠突散囊菌检测过程关键步骤探讨[J].食品科学,2017,42(11):331-335.
[17]陈然,郝彬秀,田海霞,等.六堡茶真菌分布浅析及金花菌筛选鉴定[J].食品科技,2016,41(4):19-23.
[18]陕西省卫生和计划生育委员会.DBS61/0006-2014,食品安全地方标准泾阳茯砖茶[S].2014.
[19]彭晓赟,章卫民,刘淑云,等.湖南地区茯砖茶中金花菌的分离鉴定[J].菌物研究,2011,9(3):157-161.
[20]陈桂梅,黄亚亚,梁艳,等.陕西省茯砖茶“金花”菌的鉴定分析[J].湖北农业科学,2013,55(2):345-348.
[21]谭玉梅,王亚萍,葛永怡,等.贵州地区茯砖茶“金花菌”的分离和分子鉴定[J].菌物学报,2017,36(2):154-163.