鸡源志贺氏菌的分离鉴定及耐药性研究
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摘要
昆明地区某鸡场部分鸡群突发痢疾,并有小部分鸡群死亡,疑似肠道病原菌感染所致,将采集的12份病死鸡病料进行志贺氏菌的分离鉴定、生化试验及PCR鉴定,并检测分离菌的耐药性。结果表明,共分离鉴定出志贺氏菌5株,分离菌均能发酵葡萄糖、麦芽糖、甘露醇,其中有1株菌能迟缓发酵发酵乳糖,有2株菌不发酵蔗糖;针对志贺氏菌特异性ipaH基因设计的1对引物,扩增出393 bp的目的条带,与预期大小一致;抗生素抑菌结果显示,5株志贺氏菌对加替沙星、头孢氨苄、恩诺沙星、氨苄西林和庆大霉素均产生了耐药性,并且是多重耐药,对卡那霉素和四环素的耐药程度处于中介,对氧氟沙星具有较高的敏感性。中药抑菌方面,5株分离菌对诃子、五味子和黄芩高度敏感,但是对大黄和白头翁处于低敏程度。
Some chickens on chicken farms in kunming region a sudden diarrhea, and there are a few chickens died, suspected intestinal pathogenic bacteria infection, throughout the full text,the purpose of this article is not the case, but merely the separation of bacteria identification and drug resistance analysis will collect 12 dead chicken epidemic materials for volunteers hayes bacteria isolation, biochemical test and PCR identification, identification and detection of isolated bacteria resistance. The results showed that 5 strains of shigella were isolated and identified, all of which could ferment glucose, maltose and mannitol, among which 1 strain could ferment lactose slowly, and 2 strains could not ferment sucrose. A pair of primers designed for shigella specific ipaH gene amplified the target band of 393 bp, which was consistent with the expected size. Antibiotic inhibition results showed that 5 strains of shigella were resistant to gatifloxacin, cefalexin, enoxacin, ampicillin and gentamicin, and were multidrug resistant, with intermediate resistance to kanamycin and tetracycline, and high sensitivity to ofloxacin. In the aspect of bacteriostasis of traditional Chinese medicine, 5 strains of isolates were highly sensitive to khov, schisandra, and scutellaria baicalensis, but to rhubarb and radix pulvis.
Some chickens on chicken farms in kunming region a sudden diarrhea, and there are a few chickens died, suspected intestinal pathogenic bacteria infection, throughout the full text,the purpose of this article is not the case, but merely the separation of bacteria identification and drug resistance analysis will collect 12 dead chicken epidemic materials for volunteers hayes bacteria isolation, biochemical test and PCR identification, identification and detection of isolated bacteria resistance. The results showed that 5 strains of shigella were isolated and identified, all of which could ferment glucose, maltose and mannitol, among which 1 strain could ferment lactose slowly, and 2 strains could not ferment sucrose. A pair of primers designed for shigella specific ipaH gene amplified the target band of 393 bp, which was consistent with the expected size. Antibiotic inhibition results showed that 5 strains of shigella were resistant to gatifloxacin, cefalexin, enoxacin, ampicillin and gentamicin, and were multidrug resistant, with intermediate resistance to kanamycin and tetracycline, and high sensitivity to ofloxacin. In the aspect of bacteriostasis of traditional Chinese medicine, 5 strains of isolates were highly sensitive to khov, schisandra, and scutellaria baicalensis, but to rhubarb and radix pulvis.
引文
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[5]饶桂波,孟菲,王力波,等.猪伪狂犬病毒变异株与大肠杆菌、志贺氏菌混合感染的病原鉴定及分析[J].南方农业学报,2015,46(6):1111-1116.
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[9]曾幼玲.煮沸制备质粒DNA的方法完善[J].新疆农垦科技,2006(,6):50-51.
[10]谭瑶,赵清,舒为群,等. K-B纸片扩散法药敏试验[J].检验医学与临床,2010,20(7):2290-2291.
[11]谭才邓,朱美娟,杜淑霞,等.抑菌试验中抑菌圈法的比较研究[J].食品工业,2016(,11):122-125.
[12]卫文强,吴润,刘磊.四味中草药体外抗菌作用研究[J].安徽农业科学,2009,37(6):2530-2531.
[13]庞歌.河南省部分地区鸡源志贺氏菌的分离鉴定、耐药性及PCR检测[D].郑州:河南农业大学,2010.
[14]徐司雨,李军朝,许梦怡,等.三株志贺氏菌的鉴定和药敏试验[J].黑龙江畜牧兽医,2016(,6):100-102.
[15]陈莉,朱凤静,张春枝. 30种中药水提取物的体外抗菌活性试验[J].大连工业大学学报,2005,24(4):269-271.
[2]胡秀彩,胡宏晓,吕爱军.鲤鱼肠道中沙门氏菌、志贺氏菌分离鉴定及药敏试验[J].中国畜牧兽医,2011,38(4):210-213.
[3]杨宇容.食蟹猴肠道志贺氏菌感染情况的调查[J].中国实验动物学报,2000,8(1):31-35.
[4]陈希文,郭晓萍,王雄清,等.圈养猕猴志贺氏菌的分离鉴定及药敏分析[J].四川动物,2012,31(2):32-37.
[5]饶桂波,孟菲,王力波,等.猪伪狂犬病毒变异株与大肠杆菌、志贺氏菌混合感染的病原鉴定及分析[J].南方农业学报,2015,46(6):1111-1116.
[6]尹照玉.泰安地区病死鸭胚中志贺氏菌分离鉴定及耐药性调查[D].泰安:山东农业大学,2014.
[7]王莉梅,洪淑梅.志贺菌引起暴发性食物中毒[J].中外健康文摘,2010,35(7):180-181.
[8]胡兴义,张双翔,王开功,等.一株鸡源志贺氏菌的分离、鉴定及耐药性分析[J].中国家禽,2017,39(4):56-58.
[9]曾幼玲.煮沸制备质粒DNA的方法完善[J].新疆农垦科技,2006(,6):50-51.
[10]谭瑶,赵清,舒为群,等. K-B纸片扩散法药敏试验[J].检验医学与临床,2010,20(7):2290-2291.
[11]谭才邓,朱美娟,杜淑霞,等.抑菌试验中抑菌圈法的比较研究[J].食品工业,2016(,11):122-125.
[12]卫文强,吴润,刘磊.四味中草药体外抗菌作用研究[J].安徽农业科学,2009,37(6):2530-2531.
[13]庞歌.河南省部分地区鸡源志贺氏菌的分离鉴定、耐药性及PCR检测[D].郑州:河南农业大学,2010.
[14]徐司雨,李军朝,许梦怡,等.三株志贺氏菌的鉴定和药敏试验[J].黑龙江畜牧兽医,2016(,6):100-102.
[15]陈莉,朱凤静,张春枝. 30种中药水提取物的体外抗菌活性试验[J].大连工业大学学报,2005,24(4):269-271.