橡胶树回枯病病原菌鉴定及其生物学特性测定
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摘要
对海南万宁新中农场7-33-97橡胶幼树上发生的回枯病进行病原菌鉴定、致病性及生物学特性测定。结果表明:橡胶幼树回枯病病原菌为可可毛色二孢[Lasiodiplodia theobromae(Pat.)Criff.et Maubl.]。生物学特性测定结果显示,病菌最适生长条件为温度30℃,p H值为4,碳源为葡萄糖,氮源为大豆蛋白胨;且不同光照条件对菌丝生长影响不明显。
Pathogen identification, pathogenicity and biological characteristics were carried out on dieback occurring on young rubber trees of 7-33-97 in Xinzhong Farm, Wanning, Hainan were reported in this study. The results of morphological characteristics of the pathogen and identification of molecular biology techniques showed that the pathogen was Lasiodiplodia theobromae(Pat.) Criff.et Maub. The results of biological characteristics test showed that 30 ℃, p H 4, glucose as carbon source and soybean peptone as nitrogen source were the optimum conditions for the pathogen growth. Different light conditions had little effect on the growth rate of the pathogen hypha.
Pathogen identification, pathogenicity and biological characteristics were carried out on dieback occurring on young rubber trees of 7-33-97 in Xinzhong Farm, Wanning, Hainan were reported in this study. The results of morphological characteristics of the pathogen and identification of molecular biology techniques showed that the pathogen was Lasiodiplodia theobromae(Pat.) Criff.et Maub. The results of biological characteristics test showed that 30 ℃, p H 4, glucose as carbon source and soybean peptone as nitrogen source were the optimum conditions for the pathogen growth. Different light conditions had little effect on the growth rate of the pathogen hypha.
引文
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[2]肖倩莼,余卓桐.芒果幼树回枯病研究[J].热带作物研究,1990(1):58-61.
[3]肖倩莼,黄国标,杨明兴,等.芒果树流胶病病原菌鉴定和防治研究[J].热带作物研究,1995(2):25-27.
[4]赵桂华,宋桢,何文龙.橡胶木变色菌和霉菌的研究[J].热带作物学报,1991,12(2):63-67.
[5]Encinas O,Daniell D.Degradation of the gelatinous layer in aspen and rubberwood by the blue stain fungus Lasiodiplodia theobromae[J].IAWA Journal,1997,18:107-115.
[6]Cardoso J E,Vidal J C,Dos S A A.First report of black branch dieback of cashew caused by Lasiodiplodia theobromae in Brazil[J].Plant Disease,2002,86(5):558.
[7]赵桂华.马尾松蓝变木材中长喙壳属的两个新种[J].南京林业大学学报,2005(3):113-117.
[8]郑晓慧,徐彪,何平,等.四川石榴枯萎病的病原菌[J].菌物学报,2012,31(4):523-530.
[9]方中达.植病研究方法[M].北京:中国农业出版社,1998:46,50,179-181.
[10]高秀兵,李增平,李晓娜,等.橡胶树几种根病的人工接种方法[J].热带作物学报,2010,31(4):626-630.
[11]高波,王容燕,马娟,等.甘薯爪哇黑腐病的病原鉴定[J].植物保护,2016,42(05):200-204,209.
[12]崔朝宇.江西葡萄生长后期3种烂果病害病原菌的初步研究[D].南昌:江西农业大学,2015.
[13]郭立佳,黄俊生,王国芬,等.香蕉黑腐病菌(Botryodiplodia theobromae)的PCR检测[J].植物病理学报,2007(3):248-254.
[14]陆家云.植物病原真菌学[M].北京:中国农业出版社,2001:501-502.
[15]戚佩坤.广东果树真菌病害志[M].北京:中国农业出版社,2000:18-208.
[16]胡美姣,杨冬平,杨波,等.芒果树回枯病病原菌鉴定及其生物学特性研究[J].热带作物学报,2012,33(1):122-126.
[17]彭军,张贺,张欣,等.橡胶树红根病病原菌r DNA-ITS序列鉴定[J].热带作物学报,2014,35(7):1 393-1 397.
[18]赵嘉平.树木溃疡病菌—葡萄座腔菌属及相关真菌系统分类研究[D].北京:中国林业科学研究院,2007.
[19]Punithalingam E.Plant diseases attributed to Botryodiplodia theobromae Pat[M].Berlin:Biblioteca Mycologica J,1980.
[20]Malik M T,Rahman M U,Yasmin T,et a1.Genetic diversity among Botryodiplodia theobromae isolates causing collar/stem rot of mango in Pakistan[C].International Conference on Mango and Date Palm:Culture and Export,2005.
[21]Sangeetha G,Anandan A,Rani S U.Morphological and molectilar characterization of Lasiodiplodia theobromae from various banana cultivars causing crowll rot disease in fruits[J].Archives of Phytopathology and Plant Protection,2011:1-12.
[22]Ko W H,Wang I T,Ann P J.Lasiodiplodia theobromae as a causal agent of kumquat dieback in Taiwan[J].Plant Disease,2004,88(12):1 383.
[23]Frrari F D,Ochoa C F M,Subero M L J.Dieback and gummosis induced by Lasiodiplodia theobromae(Pat.)Griffon&Maubl,on three citrus tree species[J].Anales De Bota Ilica Agricola,1996,3:46-49.
[24]Ceneno L,Pru E P.Identification of Botryodiplodia theobroma as the cause of lesions and gummosis on citrus[J].Fitopatologia Venezolana,1992,5(1):10-13.
[25]Celiker N M,Michailides T J.First report of Lasiodiplodia theobromae causing canker and shoot blight of fig in Turkey[J].New Disease Reports,2012,25:12.
[26]Safdar A,Khan S A,Safdar M A.Pathogenic association and management of Botryodiplodia theobromae in guava orchards at Sheikhupura District,Pakistan[J].International Journal of Agriculture and Biology,2015,17:297-304.
[27]Mbenoun M,Mono Z E H,Samuels G,et a1.Dieback due to Lasiodiplodia theobromae,a new constraint to cocoa production in Cameroon[J].Plant Pathology,2008,57:381.
[28]杨波,胡美姣,杨冬平,等.山竹蒂腐病病菌鉴定及其生物学特性研究[J].热带农业科学,2011,31(10):81-86.
[29]谢红辉.桑毛色二孢根腐病的病原、发生规律及其防治研究[D].南宁:广西大学,2016.
[30]Radhakrishnan N V,Ramabadran R,Jayaraj J.Botryodiplodia root rot-a new disease of mulberry[J].Indian Phytopathology,1 995,48(4):490.
[31]Prajapati H N,Patil R K.Diversity analysis of Lasiodiplodia theobromae inciting root and collar rot of Jatropha curcas using molecular tools[J].Indian Phytopathology,2015,68(3):305-310.
[32]Pereira O L,Durra D C,Dias L A.Lasiodiplodia throbromae is the causal agent of a damaging root and collar rot disease on the biofuel plant Jatropha curcas in Brazil[J].Australasian Plant Disease Notes,2009,4:120-123.
[33]Khalil O.Lasiodiplodia theobromae associated with gummosis in Eucalyptus spp.in the Sudan[J].University of Africa Journal of Sciences,2010,1:27-34.
[34]Fan M C,Yeh H C,Hong C F.First report of Lasiodiplodia theobromae causing dieback of Aquilaria sinensis in Taiwan[J].Plant Disease,2013,97(5):690.
[35]Onyeka T J,Ekpo E J A,Dixon A G O.Virulence and hostpathogen interaction of Botryodiplodia theobromae isolates of cassava root rot disease[J].Journal of Phytopathology,2005,153:726-729.
[36]Nghia N A,Chi V T Q,Dong N X.Molecular analysis of Botryodiplodia theobromae isolates from rubber in Vietnam using r DNA ITS sequencing and ISSR markers[C].IRRDB International Rubber Conference,2012:28-31.
[37]Cedeno L,Mohali S,Pru S P.Ultrastructure of Lasiodiplodia theobromae causal agent of Caribean pine blue stain in Venezuela[J].Interciencia,1996,21(5):246-271.
[38]杨卫君,王有科,赵桂华,等.杨木变色菌的分离鉴定及其生物学特性的研究[J].湖北农业科学,2009,48(5):1 225-1 228.
[39]席刚俊.杨木变色菌的分离鉴定及生防细菌的研究[D].南京:南京林业大学,2008.
[40]符永碧,施振华,利群.引起橡胶和马尾松木材蓝变的真菌—可可球二孢[J].林业科学研究,1988(2):195-200,246.
[41]黄思良,邓卫利,杨胜远,等.芒果蒂腐病菌(Botryodiplodia theobromae)生物学特性研究[J].西南农业学报,1999(1):83-87.
[42]刘媛,罗建举,任世奇,等.2种木材蓝变菌的生物学特性[J].福建林业科技,2016,43(3):76-79.
[2]肖倩莼,余卓桐.芒果幼树回枯病研究[J].热带作物研究,1990(1):58-61.
[3]肖倩莼,黄国标,杨明兴,等.芒果树流胶病病原菌鉴定和防治研究[J].热带作物研究,1995(2):25-27.
[4]赵桂华,宋桢,何文龙.橡胶木变色菌和霉菌的研究[J].热带作物学报,1991,12(2):63-67.
[5]Encinas O,Daniell D.Degradation of the gelatinous layer in aspen and rubberwood by the blue stain fungus Lasiodiplodia theobromae[J].IAWA Journal,1997,18:107-115.
[6]Cardoso J E,Vidal J C,Dos S A A.First report of black branch dieback of cashew caused by Lasiodiplodia theobromae in Brazil[J].Plant Disease,2002,86(5):558.
[7]赵桂华.马尾松蓝变木材中长喙壳属的两个新种[J].南京林业大学学报,2005(3):113-117.
[8]郑晓慧,徐彪,何平,等.四川石榴枯萎病的病原菌[J].菌物学报,2012,31(4):523-530.
[9]方中达.植病研究方法[M].北京:中国农业出版社,1998:46,50,179-181.
[10]高秀兵,李增平,李晓娜,等.橡胶树几种根病的人工接种方法[J].热带作物学报,2010,31(4):626-630.
[11]高波,王容燕,马娟,等.甘薯爪哇黑腐病的病原鉴定[J].植物保护,2016,42(05):200-204,209.
[12]崔朝宇.江西葡萄生长后期3种烂果病害病原菌的初步研究[D].南昌:江西农业大学,2015.
[13]郭立佳,黄俊生,王国芬,等.香蕉黑腐病菌(Botryodiplodia theobromae)的PCR检测[J].植物病理学报,2007(3):248-254.
[14]陆家云.植物病原真菌学[M].北京:中国农业出版社,2001:501-502.
[15]戚佩坤.广东果树真菌病害志[M].北京:中国农业出版社,2000:18-208.
[16]胡美姣,杨冬平,杨波,等.芒果树回枯病病原菌鉴定及其生物学特性研究[J].热带作物学报,2012,33(1):122-126.
[17]彭军,张贺,张欣,等.橡胶树红根病病原菌r DNA-ITS序列鉴定[J].热带作物学报,2014,35(7):1 393-1 397.
[18]赵嘉平.树木溃疡病菌—葡萄座腔菌属及相关真菌系统分类研究[D].北京:中国林业科学研究院,2007.
[19]Punithalingam E.Plant diseases attributed to Botryodiplodia theobromae Pat[M].Berlin:Biblioteca Mycologica J,1980.
[20]Malik M T,Rahman M U,Yasmin T,et a1.Genetic diversity among Botryodiplodia theobromae isolates causing collar/stem rot of mango in Pakistan[C].International Conference on Mango and Date Palm:Culture and Export,2005.
[21]Sangeetha G,Anandan A,Rani S U.Morphological and molectilar characterization of Lasiodiplodia theobromae from various banana cultivars causing crowll rot disease in fruits[J].Archives of Phytopathology and Plant Protection,2011:1-12.
[22]Ko W H,Wang I T,Ann P J.Lasiodiplodia theobromae as a causal agent of kumquat dieback in Taiwan[J].Plant Disease,2004,88(12):1 383.
[23]Frrari F D,Ochoa C F M,Subero M L J.Dieback and gummosis induced by Lasiodiplodia theobromae(Pat.)Griffon&Maubl,on three citrus tree species[J].Anales De Bota Ilica Agricola,1996,3:46-49.
[24]Ceneno L,Pru E P.Identification of Botryodiplodia theobroma as the cause of lesions and gummosis on citrus[J].Fitopatologia Venezolana,1992,5(1):10-13.
[25]Celiker N M,Michailides T J.First report of Lasiodiplodia theobromae causing canker and shoot blight of fig in Turkey[J].New Disease Reports,2012,25:12.
[26]Safdar A,Khan S A,Safdar M A.Pathogenic association and management of Botryodiplodia theobromae in guava orchards at Sheikhupura District,Pakistan[J].International Journal of Agriculture and Biology,2015,17:297-304.
[27]Mbenoun M,Mono Z E H,Samuels G,et a1.Dieback due to Lasiodiplodia theobromae,a new constraint to cocoa production in Cameroon[J].Plant Pathology,2008,57:381.
[28]杨波,胡美姣,杨冬平,等.山竹蒂腐病病菌鉴定及其生物学特性研究[J].热带农业科学,2011,31(10):81-86.
[29]谢红辉.桑毛色二孢根腐病的病原、发生规律及其防治研究[D].南宁:广西大学,2016.
[30]Radhakrishnan N V,Ramabadran R,Jayaraj J.Botryodiplodia root rot-a new disease of mulberry[J].Indian Phytopathology,1 995,48(4):490.
[31]Prajapati H N,Patil R K.Diversity analysis of Lasiodiplodia theobromae inciting root and collar rot of Jatropha curcas using molecular tools[J].Indian Phytopathology,2015,68(3):305-310.
[32]Pereira O L,Durra D C,Dias L A.Lasiodiplodia throbromae is the causal agent of a damaging root and collar rot disease on the biofuel plant Jatropha curcas in Brazil[J].Australasian Plant Disease Notes,2009,4:120-123.
[33]Khalil O.Lasiodiplodia theobromae associated with gummosis in Eucalyptus spp.in the Sudan[J].University of Africa Journal of Sciences,2010,1:27-34.
[34]Fan M C,Yeh H C,Hong C F.First report of Lasiodiplodia theobromae causing dieback of Aquilaria sinensis in Taiwan[J].Plant Disease,2013,97(5):690.
[35]Onyeka T J,Ekpo E J A,Dixon A G O.Virulence and hostpathogen interaction of Botryodiplodia theobromae isolates of cassava root rot disease[J].Journal of Phytopathology,2005,153:726-729.
[36]Nghia N A,Chi V T Q,Dong N X.Molecular analysis of Botryodiplodia theobromae isolates from rubber in Vietnam using r DNA ITS sequencing and ISSR markers[C].IRRDB International Rubber Conference,2012:28-31.
[37]Cedeno L,Mohali S,Pru S P.Ultrastructure of Lasiodiplodia theobromae causal agent of Caribean pine blue stain in Venezuela[J].Interciencia,1996,21(5):246-271.
[38]杨卫君,王有科,赵桂华,等.杨木变色菌的分离鉴定及其生物学特性的研究[J].湖北农业科学,2009,48(5):1 225-1 228.
[39]席刚俊.杨木变色菌的分离鉴定及生防细菌的研究[D].南京:南京林业大学,2008.
[40]符永碧,施振华,利群.引起橡胶和马尾松木材蓝变的真菌—可可球二孢[J].林业科学研究,1988(2):195-200,246.
[41]黄思良,邓卫利,杨胜远,等.芒果蒂腐病菌(Botryodiplodia theobromae)生物学特性研究[J].西南农业学报,1999(1):83-87.
[42]刘媛,罗建举,任世奇,等.2种木材蓝变菌的生物学特性[J].福建林业科技,2016,43(3):76-79.