高频电针对心肌缺血损伤大鼠经穴及非穴皮肤温度的影响
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摘要
目的借助大鼠心肌缺血损伤模型,探讨高频电针刺激"内关"穴或非穴后,相关经穴、非相关经穴及非区穴皮肤温度的变化规律及其随时间的变化情况。方法雄性Wistar大鼠随机分为空白对照组、假手术组、心肌缺血损伤组(简称模型组)、高频电针经穴组和高频电针非穴组。高频电针经穴组、高频电针非穴组均在成功制备模型的基础上给予电针治疗。于第3天电针治疗后30、60 min,应用红外热成像仪记录"内关"穴、"阳陵泉"穴、非穴区皮肤红外热像图并进行数据统计分析。结果与空白对照组比较,模型组"内关"穴区皮肤温度显著降低(P<0.01)。与模型组比较,高频电针经穴组"内关"穴区皮肤温度升高(P<0.01),高频电针非穴组"内关"穴、"阳陵泉"穴、非穴区皮肤温度差异无统计学意义;其中高频电针经穴组"内关"穴区皮肤温度以治疗后30 min更接近空白对照组。结论 "内关"穴区皮肤温度能相对特异性地反映大鼠心肌所处的不同状态,高频电针"内关"穴能阻抑由于心肌缺血损伤诱发的相关经穴皮肤温度的下降,其机制有待进一步研究。
Objective To compare the skin temperature around relevant and irrelevant meridian acupoints,or non-meridian sham acupoints in rats with myocardial ischemia by high frequency electro-acupuncture( EA) at Neiguan( PC6) point or sham acupoint,in healthy rats and myocardial ischemic rats.Methods Fifty male Wister rats were randomly divided into five groups: blank control group,sham-operation group,model group,high frequency EA at Neiguan group( PC6) and high frequency electro-acupuncture at sham acupoint groups. Rats in high frequency EA at and at sham acupoints group received EA intervention after their models were successfully established. Temperature around the skin of Neiguan( PC6),Yanglingquan( GB34) and sham acupoints were measured and recorded by infrared thermogra-phy in every group at 30 minutes 60 minutes after three-day treatment for statistical analysis. Results Compared with the blank control group,skin temperature around PC6 in model groups was significantly lower( P < 0. 01); Compared with the model group,skin temperature around PC6 in high frequency EA at PC6 group was significantly increased( P < 0. 01); Skin temperature around PC6,GB34 and sham acupoint in high frequency EA at sham acupoint groups presented with no statistical significance; skin temperature around PC6 in high frequency EA at PC6 group was similar to the skin temperature of blank control group,which was measured 30 minutes after the treatment. Conclusion The relative specificity of skin temperature around PC6 can be useful for the reflection of different conditions of rats' myocardium. High-frequency electro-acupuncture at PC6 can prevent the reduction of skin temperature caused by myocardial ischemia injury,of which the mechanism needs further study.
Objective To compare the skin temperature around relevant and irrelevant meridian acupoints,or non-meridian sham acupoints in rats with myocardial ischemia by high frequency electro-acupuncture( EA) at Neiguan( PC6) point or sham acupoint,in healthy rats and myocardial ischemic rats.Methods Fifty male Wister rats were randomly divided into five groups: blank control group,sham-operation group,model group,high frequency EA at Neiguan group( PC6) and high frequency electro-acupuncture at sham acupoint groups. Rats in high frequency EA at and at sham acupoints group received EA intervention after their models were successfully established. Temperature around the skin of Neiguan( PC6),Yanglingquan( GB34) and sham acupoints were measured and recorded by infrared thermogra-phy in every group at 30 minutes 60 minutes after three-day treatment for statistical analysis. Results Compared with the blank control group,skin temperature around PC6 in model groups was significantly lower( P < 0. 01); Compared with the model group,skin temperature around PC6 in high frequency EA at PC6 group was significantly increased( P < 0. 01); Skin temperature around PC6,GB34 and sham acupoint in high frequency EA at sham acupoint groups presented with no statistical significance; skin temperature around PC6 in high frequency EA at PC6 group was similar to the skin temperature of blank control group,which was measured 30 minutes after the treatment. Conclusion The relative specificity of skin temperature around PC6 can be useful for the reflection of different conditions of rats' myocardium. High-frequency electro-acupuncture at PC6 can prevent the reduction of skin temperature caused by myocardial ischemia injury,of which the mechanism needs further study.
引文
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[7]戴健,嵇波,路雅雯,等.大鼠不同心肌状态对相关经穴和非相关经穴皮肤温度的影响[J].长春中医药大学学报,2017,33(2):208-211.Dai J,Ji B,Lu YW,et al.Effect of myocardium in rats with different states on the skin temperature changes of heart-related acupoints and heart-irrelevant acupoints[J].Journal of Changchun University of Chinese Medicine,2017,33(2):208-211.
[8]张栋,王淑友,马惠敏,等.内脏病变(心包炎)体表循经温度反应的红外热像图显示[J].中国中医基础医学杂志,2004,10(7):52-54.Zhang D,Wang SY,Ma HM,et al.Infrared thermography of body surface temperature response to visceral lesions(pericarditis)[J].Chinese Journal of Basic Medicine in Traditional Chinese Medicine,2004,10(7):52-54.
[9]陈冰俊,屈箫箫,张栋,等.针灸调整面瘫患者面部穴位温度对称性观察[J].中国中医基础医学杂志,2011,17(5):556-557.Chen BJ,Qu XX,Zhang D,et al.Observation on symmetry of facial acupoint temperature in patients with facial paralysis by Acupuncture[J].Chinese Journal of Basic Medicine in Traditional Chinese Medicine,2011,17(5):556-557.
[10]唐丽梅,王淑友,李顺月,等.电针合谷对面部温度和迎香穴区经皮氧分压的影响[J].中国中医基础医学杂志,2014,20(9):1277-1280.Tang LM,Wang SY,Li SY,et al.Effects of superficial temperature and transcutaneous oxygen pressure of Yingxiang point induced by EA Hegu[J].Chinese Journal of Basic Medicine in Traditional Chinese Medicine,2014,20(9):1277-1280.
[11]杨佳敏,沈小雨,罗丽,等.针刺不同穴位对寒凝证类痛经大鼠体表区域温度的影响[J].医学研究生学报,2014,27(9):900-904.Yang JM,Shen XY,Luo L,et al.Effects of needling different acupoints on skin temperature in SP6,SP10 and CV4 in the rat model of cold congealing and dysmenorrhea[J].J Med Postgra,2014,27(9):900-904.
[12]沈雪勇,丁光宏,邓海平,等.冠心病患者内关穴红外辐射光谱病理信息分析[J].红外与毫米波学报,2006,25(6):443-446.Shen XY,Ding GH,Deng HP,et al.Analysis on pathological information of infrared radiation spectrums at acupuncture point Neiguan(PC6)for patients with coronary heart disease[J].J Infrared Millim Waves,2006,25(6):443-446.
[13]邓海平,沈雪勇,丁光宏,等.太渊穴自发红外辐射的相对特异性[J].中华中医药学刊,2008,26(3):494-495.Deng HP,Shen XY,Ding GH,et al.Study on the speciality of the infrared radiation at Taiyuan(LU9)[J].Chinese archives of traditional Chinese medicine,2008,26(3):494-495.
[14]王树东,董宝强,陈文娜,等.电针“内关”穴对心肌缺血大鼠TXB2、PGFIα和心肌形态影响的实验研究[J].辽宁中医杂志,2015,42(2):413-414.Wang SD,Dong BQ,Chen WN,et al.Experimental study of electro-acupuncture at Neiguan point on TXB2,PGFIαand myocardial morphology in rats with myocardial ischemia[J].Liaoning Journal of Traditional Chinese Medicine,2015,42(2):413-414.
[2]郭义,陈爽白,张春煦,等.健康人体经穴Ca2+浓度分布特异性的观察[J].上海针灸杂志,2002,21(1):37-38.Guo Y,Chen SB,Zhang CX,et al.Specific distribution of Ca2+concentration in acupoints of healthy human bodies[J].Shanghai J Acu-mox,2002,21(1):37-38.
[3]王淑友,张栋,朱元根,等.穴位温度与电阻相关关系的研究[J].辽宁中医杂志,2007,34(1):5-6.Wang SY,Zhang D,Zhu YG,et al.Research on the correlation between acupoint temperature and resistance[J].Liaoning Journal of Traditional Chinese Medicine,2007,34(1):5-6.
[4]嵇波,胡琅琳,李晓泓,等.急性心肌缺血损伤后大鼠穴区NO和NOS的变化及不同频率电针的干预作用[J].北京中医药大学学报,2012,35(8):563-566.Ji B,Hu LL,Li XH,et al.Changes of NO and NOS around acupoints and intervention of electro-acupuncture with various frequency in rats with acute myocardial ischemia[J].Journal of Beijing University of Traditional Chinese Medicine,2012,35(8):563-566.
[5]岑珏,赵影,陈跃来.膀胱过度活动症患者中极穴红外辐射温度特性研究[J].上海针灸杂志,2012,31(6):438-440.Chen J,Zhao Y,Chen YL.Study of the infrared radiation temperature characteristic of point Zhongji(CV3)in overactive bladder patients[J].Shanghai J Acu-mox,2012,31(6):438-440.
[6]黄日龙,张伟,东贵荣.电针内关穴对急性心肌缺血家兔心肌组织能量代谢的实验研究[J].针灸临床杂志,2010,26(12):44-48.Huang RL,Zhang W,Dong GR.The experimental study of effectiveness on energy metabolism of cardiac muscle of acute heart muscle ischemic a rabbits by means of“Neiguan”electroacupuncture[J].JCAM,2010,26(12):44-48.
[7]戴健,嵇波,路雅雯,等.大鼠不同心肌状态对相关经穴和非相关经穴皮肤温度的影响[J].长春中医药大学学报,2017,33(2):208-211.Dai J,Ji B,Lu YW,et al.Effect of myocardium in rats with different states on the skin temperature changes of heart-related acupoints and heart-irrelevant acupoints[J].Journal of Changchun University of Chinese Medicine,2017,33(2):208-211.
[8]张栋,王淑友,马惠敏,等.内脏病变(心包炎)体表循经温度反应的红外热像图显示[J].中国中医基础医学杂志,2004,10(7):52-54.Zhang D,Wang SY,Ma HM,et al.Infrared thermography of body surface temperature response to visceral lesions(pericarditis)[J].Chinese Journal of Basic Medicine in Traditional Chinese Medicine,2004,10(7):52-54.
[9]陈冰俊,屈箫箫,张栋,等.针灸调整面瘫患者面部穴位温度对称性观察[J].中国中医基础医学杂志,2011,17(5):556-557.Chen BJ,Qu XX,Zhang D,et al.Observation on symmetry of facial acupoint temperature in patients with facial paralysis by Acupuncture[J].Chinese Journal of Basic Medicine in Traditional Chinese Medicine,2011,17(5):556-557.
[10]唐丽梅,王淑友,李顺月,等.电针合谷对面部温度和迎香穴区经皮氧分压的影响[J].中国中医基础医学杂志,2014,20(9):1277-1280.Tang LM,Wang SY,Li SY,et al.Effects of superficial temperature and transcutaneous oxygen pressure of Yingxiang point induced by EA Hegu[J].Chinese Journal of Basic Medicine in Traditional Chinese Medicine,2014,20(9):1277-1280.
[11]杨佳敏,沈小雨,罗丽,等.针刺不同穴位对寒凝证类痛经大鼠体表区域温度的影响[J].医学研究生学报,2014,27(9):900-904.Yang JM,Shen XY,Luo L,et al.Effects of needling different acupoints on skin temperature in SP6,SP10 and CV4 in the rat model of cold congealing and dysmenorrhea[J].J Med Postgra,2014,27(9):900-904.
[12]沈雪勇,丁光宏,邓海平,等.冠心病患者内关穴红外辐射光谱病理信息分析[J].红外与毫米波学报,2006,25(6):443-446.Shen XY,Ding GH,Deng HP,et al.Analysis on pathological information of infrared radiation spectrums at acupuncture point Neiguan(PC6)for patients with coronary heart disease[J].J Infrared Millim Waves,2006,25(6):443-446.
[13]邓海平,沈雪勇,丁光宏,等.太渊穴自发红外辐射的相对特异性[J].中华中医药学刊,2008,26(3):494-495.Deng HP,Shen XY,Ding GH,et al.Study on the speciality of the infrared radiation at Taiyuan(LU9)[J].Chinese archives of traditional Chinese medicine,2008,26(3):494-495.
[14]王树东,董宝强,陈文娜,等.电针“内关”穴对心肌缺血大鼠TXB2、PGFIα和心肌形态影响的实验研究[J].辽宁中医杂志,2015,42(2):413-414.Wang SD,Dong BQ,Chen WN,et al.Experimental study of electro-acupuncture at Neiguan point on TXB2,PGFIαand myocardial morphology in rats with myocardial ischemia[J].Liaoning Journal of Traditional Chinese Medicine,2015,42(2):413-414.