两种麦冬传统性状与显微特征的关联性研究
|
摘要
目的寻找麦冬传统性状与显微特征的关联性,为麦冬传统经验鉴别提供组织学依据。方法采用性状和显微鉴定方法对湖北麦冬、川麦冬进行比较,采用灰色关联分析对其性状和显微鉴定结果进行关联性分析。结果灰色关联分析显示:麦冬中柱直径与木质部束个数关系最为密切,关联度为0.85,即中柱直径越大,则木质部束个数越多,导致麦冬断面木芯明显;性状和显微鉴定显示:两种麦冬表皮皱缩深浅度的不同与其根被细胞数目及列数排布的均匀性有关。结论将性状鉴定、显微鉴定和数学分析方法相结合,可科学诠释性状差异与内部组织结构的相关性,为阐明中药传统经验鉴别精髓提供新的思路和方法。
Objective To find out the correlation between the traditional traits and microscopic features of Radix Ophiopogonis, and provide the histological basis for empirical identification.Methods Hubei Radix Ophiopogonis and Chuan Radix Ophiopogonis were compared by macroscopic identification and microscopic identification, the correlation of the macroscopic identification and microscopic identification results was performed using grey correlation analysis. Results The grey relational analysis showed that the diameter of the vascular cylinder was closely related to the number of xylem bundles, and the correlation degree is 0.85, If the diameter of the vascular cylinder is larger, the number of xylem bundles will increase, leading to obvious wood core of the Radix Ophiopogonis section; The macroscopic and microscopic identification showed that the difference shrinkage between the two kinds of Radix Ophiopogonis epidermis was related to the number of velamen cell and the distributed uniformity. Conclusion The combination of macroscopic identification, microscopic identification and mathematical analysis methods can scientifically interpret the correlation between trait and internal organizational structure,and provided new ideas and methods for elucidating the essence of empirical identification of traditional Chinese medicine.
Objective To find out the correlation between the traditional traits and microscopic features of Radix Ophiopogonis, and provide the histological basis for empirical identification.Methods Hubei Radix Ophiopogonis and Chuan Radix Ophiopogonis were compared by macroscopic identification and microscopic identification, the correlation of the macroscopic identification and microscopic identification results was performed using grey correlation analysis. Results The grey relational analysis showed that the diameter of the vascular cylinder was closely related to the number of xylem bundles, and the correlation degree is 0.85, If the diameter of the vascular cylinder is larger, the number of xylem bundles will increase, leading to obvious wood core of the Radix Ophiopogonis section; The macroscopic and microscopic identification showed that the difference shrinkage between the two kinds of Radix Ophiopogonis epidermis was related to the number of velamen cell and the distributed uniformity. Conclusion The combination of macroscopic identification, microscopic identification and mathematical analysis methods can scientifically interpret the correlation between trait and internal organizational structure,and provided new ideas and methods for elucidating the essence of empirical identification of traditional Chinese medicine.
引文
[1] 赵中振,陈虎彪,肖培根,等.传统中药鉴定学科的继承与创新[J].中国中药杂志,2015,40(17):3385.
[2] 张静,张建逵,王冰,等.基于生物计量学的野菊花及其伪品的鉴别研究[J].时珍国医国药,2014,25(12):2918.
[3] Choy S K,Tong C S,Zhao Z Z.A novel and effective multistage classification system for microscopic starch grain images[J].Microsc Res Tech,2010,73(1):77.
[4] 康廷国.中药鉴定学[M].北京:中国中医药出版社,2012:201.
[5] 莫建霞.常用商品麦冬类药材的比较鉴别[J].中药材.2010,33(7):1074.
[6] 刘霞,曹秀荣,陈科力,等.湖北麦冬的研究进展[J].医药导报,2008,27(10):1231.
[7] 吴发明,杨瑞山,李敏,等.麦冬主流品种药材质量比较研究[J].中国药学杂志,2017,52(6):447.
[8] 林以宁,朱丹妮,寇俊萍,等.麦冬类药材皂苷元含量与其抑制中性粒细胞呼吸爆发的相关性[J].中国药科大学学报,2007,38(6):549.
[9] 魏从师,郑虹.麦冬与山麦冬的鉴定使用[J].北方药学,2011,8(3):80.
[10] 叶宝兴,毕建杰,孙印石.植物细胞与组织研究方法[M].北京:化学工业出版社,2011.
[11] 梁健钦,王剑,熊万娜,等.基于灰色关联分析的芒果叶提取物抗炎作用的谱效关系[J].中国实验方剂学杂志,2015,21(1):121.
[12] 秦雪梅,孔增科,张丽增,等.中药材"辨状论质"解读及商品规格标准研究思路[J].中草药,2012,43(11):2093.
[2] 张静,张建逵,王冰,等.基于生物计量学的野菊花及其伪品的鉴别研究[J].时珍国医国药,2014,25(12):2918.
[3] Choy S K,Tong C S,Zhao Z Z.A novel and effective multistage classification system for microscopic starch grain images[J].Microsc Res Tech,2010,73(1):77.
[4] 康廷国.中药鉴定学[M].北京:中国中医药出版社,2012:201.
[5] 莫建霞.常用商品麦冬类药材的比较鉴别[J].中药材.2010,33(7):1074.
[6] 刘霞,曹秀荣,陈科力,等.湖北麦冬的研究进展[J].医药导报,2008,27(10):1231.
[7] 吴发明,杨瑞山,李敏,等.麦冬主流品种药材质量比较研究[J].中国药学杂志,2017,52(6):447.
[8] 林以宁,朱丹妮,寇俊萍,等.麦冬类药材皂苷元含量与其抑制中性粒细胞呼吸爆发的相关性[J].中国药科大学学报,2007,38(6):549.
[9] 魏从师,郑虹.麦冬与山麦冬的鉴定使用[J].北方药学,2011,8(3):80.
[10] 叶宝兴,毕建杰,孙印石.植物细胞与组织研究方法[M].北京:化学工业出版社,2011.
[11] 梁健钦,王剑,熊万娜,等.基于灰色关联分析的芒果叶提取物抗炎作用的谱效关系[J].中国实验方剂学杂志,2015,21(1):121.
[12] 秦雪梅,孔增科,张丽增,等.中药材"辨状论质"解读及商品规格标准研究思路[J].中草药,2012,43(11):2093.