猪NLRP3蛋白的原核表达与多克隆抗体制备
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摘要
为了获得猪源NLRP3重组蛋白以及针对猪NLRP3蛋白的多克隆抗体,本试验采用原核表达技术对猪源NLRP3蛋白的主要抗原区域进行了截短表达与鉴定,并以重组蛋白为免疫原免疫兔制备兔抗NLRP3蛋白多克隆抗体。结果显示,经RT-PCR方法扩增到大小为864 bp的NLRP3蛋白的主要抗原区域基因;将目的片段定向克隆至pET-32α原核表达载体,转化大肠埃希菌BL21,经IPTG诱导获得了大小为50 000的重组蛋白;重组蛋白经纯化后Western blot检测其可与抗His标签单克隆抗体发生特异性反应;以纯化蛋白为免疫原制备的兔抗NLRP3蛋白多克隆抗体的抗体效价达1∶25 600,Western blot检测其可与NLRP3重组蛋白发生特异性反应。结果表明,获得了具有良好反应活性的NLRP3重组蛋白与多克隆抗体,为研究NLRP3蛋白的结构与功能、NLRP3蛋白与猪炎症性疾病的相互作用机制奠定了基础。
in order to obtain the recombinant protein of the pig source NLRP3 and the polyclonal antibody against the pig NLRP3 protein,the primary antigen region of the pig source NLRP3 protein was truncated and identified by prokaryotic expression technology,and the rabbit anti NLRP3 protein polyclonal antibody was prepared by the recombinant protein as immunogen.The results showed that the main antigen region gene of NLRP3 protein with 864 bp was amplified by RT-PCR,and the target fragment was cloned into the pET-32α prokaryotic expression vector,the Escherichia coli BL21 was transformed and the recombinant protein of 50 000 was induced by IPTG.The recombinant protein was purified and detected by Western blot,which could react to the anti His tag monoclonal antibody,the antibody titer of the rabbit anti NLRP3 protein polyclonal antibody prepared by the purified protein was 1∶25 600.Western blot detection showed that it could react specifically to NLRP3 recombinant protein.The results indicated that the recombinant protein and polyclonal antibody of NLRP3 with good reactive activity were obtained,which laid the foundation for the study of the structure and function of NLRP3 protein and the interaction mechanism of NLRP3 protein with swine inflammatory diseases.
in order to obtain the recombinant protein of the pig source NLRP3 and the polyclonal antibody against the pig NLRP3 protein,the primary antigen region of the pig source NLRP3 protein was truncated and identified by prokaryotic expression technology,and the rabbit anti NLRP3 protein polyclonal antibody was prepared by the recombinant protein as immunogen.The results showed that the main antigen region gene of NLRP3 protein with 864 bp was amplified by RT-PCR,and the target fragment was cloned into the pET-32α prokaryotic expression vector,the Escherichia coli BL21 was transformed and the recombinant protein of 50 000 was induced by IPTG.The recombinant protein was purified and detected by Western blot,which could react to the anti His tag monoclonal antibody,the antibody titer of the rabbit anti NLRP3 protein polyclonal antibody prepared by the purified protein was 1∶25 600.Western blot detection showed that it could react specifically to NLRP3 recombinant protein.The results indicated that the recombinant protein and polyclonal antibody of NLRP3 with good reactive activity were obtained,which laid the foundation for the study of the structure and function of NLRP3 protein and the interaction mechanism of NLRP3 protein with swine inflammatory diseases.
引文
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[11] 张涵,马晶,张云凌,等.肺炎支原体感染RAW264.7细胞NLRP3炎性体及下游分子的表达[J].中国病原生物学杂志,2014,9(6):496-499.
[2] JACOBS S R,DAMANIA B.NLRs,inflammasomes,and viral infection[J].J Leukoc Biol,2012,92(3):469-477.
[3] 蒋丹露,刘阳阳,孙如愚,等.NLRP3炎症小体介导的炎症相关疾病研究进展[J].生命科学,2017,29(9):898-907.
[4] 郑金娟,金丹.NLRP3炎症小体的研究进展[J].延边大学医学学报,2017,40(4):302-304.
[5] WANG S L,ZHAO G,ZHU W,et al.Herpes simplex virus-1 infection or Simian virus 40-mediated immortalization of corneal cellscauses permanent translocation of NLRP3 to the nuclei[J].Int J Ophthalmol,2015,8(1):46-51.
[6] OZAKI E,CAMPBELL M,DOYLE S L.Targeting the NLRP3 inflammasome in chronic inflammatory diseases:current perspectives[J].J Inflamm Res,2015(8):15-27.
[7] 陶攀,张凯照,刘健新,等.猪NLRP3基因克隆及其在DF-1细胞的表达[J].中国兽医学报,2017,37(4):735-740.
[8] KIM J J,JO E K.NLRP3 inflammasome and host protection against bacterial infection[J].J Korean Med Sci,2013,28(10):1415,1423.
[9] 金芬芬,宋若会,屠彦红.NLRP3炎性小体研究进展[J].中医药临床杂志,2018(3):564-567.
[10] 王文秀,张艳,王宝琴,等.巴马香猪氨基肽酶N截短基因的克隆与原核表达[J].动物医学进展,2017,38(8):29-33.
[11] 张涵,马晶,张云凌,等.肺炎支原体感染RAW264.7细胞NLRP3炎性体及下游分子的表达[J].中国病原生物学杂志,2014,9(6):496-499.