鳙鱼鱼鳔黏多糖的提取及其抗氧化性活性评价
|
摘要
【目的】探讨鳙鱼(Aristichthys nobilis)鱼鳔黏多糖的提取工艺及提取物的化学组成及抗氧化活性。【方法】采用热水浸提法和碱液浸提法提取黏多糖,用GC-MS法测定分析所提取多糖的化学组成和抗氧化活性等性质。【结果】碱液浸提法的最佳工艺条件为料液质量体积比(g/m L)1∶20、碱液质量分数1%、提取温度70℃、提取时间为4 h,在此条件下,黏多糖的提取率为5.58%;热水浸提和碱液浸提得到的鳙鱼鱼鳔黏多糖量分别为5.3%和25.33%;气质联用色谱(GC-MC)测定结果显示,热水提取的黏多糖(WANVM)主要由质量分数13.72%木糖、19.57%甘露糖、16.11%葡萄糖及32.49%半乳糖构成;碱液提取的黏多糖(AANVM)主要由质量分数50%甘露糖和34.87%半乳糖构成。在相同质量分数条件下,WANVM清除羟基自由基、DPPH自由基及总还原力均高于AANVM。【结论】碱液浸提对黏多糖的提取效果优于热水浸提,AANVM、WANVM组分不同,且前者抗氧化活性略高于后者。
【Objective】 The technology of extracting mucopolysaccharide from Aristichthys nobilis swim bladder, the chemical characteristics, and antioxidantion properties of the extracted mucopolysaccharide were analyzed.【Methods】Mucopolysaccharide was extracted from Aristichthys nobilis swim bladder by hot water and alkali method. 【Results】The optimum processing conditions of alkali extraction are material(g) to solution(m L) ratio 1∶20, 1% alkali, 70 ℃ and 4 h with the extraction rate 5.58% The polysaccharide mass fraction obtained by hot water and alkali extraction were 42.75% and 54.78%. The GC-MC analysis showed that hot water extract of mucopolysaccharide(WANVM) by GC-MC analysis mainly contained xylose(13.72%), mannose(19.57%), glucose(16.11%) and galactose(32.49%), and the alkali extract of mucopolysaccharide(AANVM) mainly contained mannose(50%) and galactose(34.87%). As compared to WANVM, AANVM showed higher OH-scavenging activity, DPPH-scavenging activity and reducing power with the same concentration.【Conclusion】The mucopolysaccharide extract obtained by alkali method is better than that by hot water extraction method. Moreover, the composition of AANVM and WANVM, is different, with the antioxidant activity of the former slightly higher than that of the later.
【Objective】 The technology of extracting mucopolysaccharide from Aristichthys nobilis swim bladder, the chemical characteristics, and antioxidantion properties of the extracted mucopolysaccharide were analyzed.【Methods】Mucopolysaccharide was extracted from Aristichthys nobilis swim bladder by hot water and alkali method. 【Results】The optimum processing conditions of alkali extraction are material(g) to solution(m L) ratio 1∶20, 1% alkali, 70 ℃ and 4 h with the extraction rate 5.58% The polysaccharide mass fraction obtained by hot water and alkali extraction were 42.75% and 54.78%. The GC-MC analysis showed that hot water extract of mucopolysaccharide(WANVM) by GC-MC analysis mainly contained xylose(13.72%), mannose(19.57%), glucose(16.11%) and galactose(32.49%), and the alkali extract of mucopolysaccharide(AANVM) mainly contained mannose(50%) and galactose(34.87%). As compared to WANVM, AANVM showed higher OH-scavenging activity, DPPH-scavenging activity and reducing power with the same concentration.【Conclusion】The mucopolysaccharide extract obtained by alkali method is better than that by hot water extraction method. Moreover, the composition of AANVM and WANVM, is different, with the antioxidant activity of the former slightly higher than that of the later.
引文
[1]邬冠鹏.鱼鳔肽的开发与利用[C]//亚太多肽学会多肽抗肿瘤国际论坛论文集,中国武汉,2004:4.http://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=IPF D&dbname=IPFD9914&filename=ZGBJ200410003023&uid=WEEv REcw Sl JHSld Ra1Fhc EE0RVZxej Rid Ggz M0d Wd XNn Qm N4WDl Zen VSND0=$9A4h F_YAuv Q5obg V Aq NKPCYc Ej Kens W4gg I8Fm4g Tko UKa ID8j8g Fw!!&v=MDQ3NTd4Tkt1a GRobmo5OFRuan Fxe GRFZU1PVUt ya WZad TV2Rl Nqb VU3dkl Kb HNRUHly Slp MRz RId Fh O cj Q5Rlorc05E.
[2]ZHAO X,QING Y,LI G J,et al.Preventive effects of the polysaccharide of Larimichthys crocea swim bladder on carbon tetrachloride(CCl4)-induced hepatic damage[J].Chinese Journal of Natural Medicines,2015,13(7):521-528.
[3]SUO H Y,SONG J L,ZHOU Y L,et al.Induction of apoptosis in HCT-116 colon cancer cells by polysaccharide of Larimichthys crocea swim bladder[J].Oncology Letters,2015,9(2):972-978.
[4]JIANG X H,ZHAO X,LUO H L,et al.Therapeutic effect of polysaccharide of large yellow croaker swim bladder on lupus nephritis of mice[J].Nutrients,2014,6(3):1223-1235.
[5]段振华,汪菊兰,殷安齐,等.几种鱼鳔的营养成分分析与评价[J].食品研究与开发,2007,28(10):62-65.
[6]扈瑞平,张兴夫,杜玲,等.沙葱多糖Sevage法除蛋白工艺的研究[J].内蒙古大学学报(自然科学版),2009,40(6):658-662.
[7]DUBOIS M,GILLES K A,HAMILTON J K,et al.Colorimetric method for determination of sugars and related substances[J].Analytical Chemistry,1956,28(3):350-356.
[8]张惟杰.糖复合物生化研究技术[M].2版.杭州:浙江大学出版社,1999:413-414.
[9]许会生,张铁军,赵广荣,等.一种测定酸性多糖中糖醛酸和中性糖含量的改良方法[J].食品工业科技,2007,28(7):197-199.
[10]DIETRICH C P,MCDUFFIE N M,SAMPAIO L O.Identification of acidic mucopolysaccharides by agarose gel electrophoresis[J].Journal of Chromatography A,1977,130:299-304.
[11]宋珅,赵保堂,殷振雄,等.籽瓜多糖的提取分离及单糖组成的GC-MS分析[J].食品与发酵工业,2013,39(5):238-245.
[12]SHIMADA K,FUJIKAWA K,YAHAR K,et al.Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion[J].Journal of Agricultural and Food Chemistry,1992,40(6):945-948.
[13]LIU X,SUN Z L,ZHANG M S,et al.Antioxidant and antihyperlipidemic activities of polysaccharides from sea cucumber Apostichopus japonicus[J].Carbohydrate Polymers,2012,90(4):1664-1670.
[14]董晓静.波纹巴非蛤糖胺聚糖抗氧化与降血脂活性研究[D].湛江:广东海洋大学,2010.
[15]OYAIZU M.Studies on product of browning reaction prepared from glucose amine[J].Japanese journal of Nutrition,1986,44(6):307-315.
[16]刘义,钱和.糖胺聚糖的提取、分离方法[J].海洋水产研究,2005,26(4):89-93.
[17]QIAO D L,KE C L,HU B,et al.Antioxidant activities of polysaccharides from Hyriopsis cumingii[J].Carbohydrate Polymers,2009,78(2):199-204.
[18]罗晓航.PEF结合酶法提取鲍鱼脏器粗多糖及其抗氧化活性研究[D].福州:福建农林大学,2012.
[19]孙丽平,鲍长俊,柳建华,等.乌鳢分泌多糖和黏多糖的提取及其抗氧化活性研究[J].食品与发酵工业,2015,41(7):81-85.
[20]孙晓朋.四角蛤蜊糖胺聚糖的提取、纯化及生理活性研究[D].天津:天津科技大学,2011.
[21]许桂芹.福寿螺多糖的分离纯化及其生物活性研究[D].福州:福建农林大学,2008.
[2]ZHAO X,QING Y,LI G J,et al.Preventive effects of the polysaccharide of Larimichthys crocea swim bladder on carbon tetrachloride(CCl4)-induced hepatic damage[J].Chinese Journal of Natural Medicines,2015,13(7):521-528.
[3]SUO H Y,SONG J L,ZHOU Y L,et al.Induction of apoptosis in HCT-116 colon cancer cells by polysaccharide of Larimichthys crocea swim bladder[J].Oncology Letters,2015,9(2):972-978.
[4]JIANG X H,ZHAO X,LUO H L,et al.Therapeutic effect of polysaccharide of large yellow croaker swim bladder on lupus nephritis of mice[J].Nutrients,2014,6(3):1223-1235.
[5]段振华,汪菊兰,殷安齐,等.几种鱼鳔的营养成分分析与评价[J].食品研究与开发,2007,28(10):62-65.
[6]扈瑞平,张兴夫,杜玲,等.沙葱多糖Sevage法除蛋白工艺的研究[J].内蒙古大学学报(自然科学版),2009,40(6):658-662.
[7]DUBOIS M,GILLES K A,HAMILTON J K,et al.Colorimetric method for determination of sugars and related substances[J].Analytical Chemistry,1956,28(3):350-356.
[8]张惟杰.糖复合物生化研究技术[M].2版.杭州:浙江大学出版社,1999:413-414.
[9]许会生,张铁军,赵广荣,等.一种测定酸性多糖中糖醛酸和中性糖含量的改良方法[J].食品工业科技,2007,28(7):197-199.
[10]DIETRICH C P,MCDUFFIE N M,SAMPAIO L O.Identification of acidic mucopolysaccharides by agarose gel electrophoresis[J].Journal of Chromatography A,1977,130:299-304.
[11]宋珅,赵保堂,殷振雄,等.籽瓜多糖的提取分离及单糖组成的GC-MS分析[J].食品与发酵工业,2013,39(5):238-245.
[12]SHIMADA K,FUJIKAWA K,YAHAR K,et al.Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion[J].Journal of Agricultural and Food Chemistry,1992,40(6):945-948.
[13]LIU X,SUN Z L,ZHANG M S,et al.Antioxidant and antihyperlipidemic activities of polysaccharides from sea cucumber Apostichopus japonicus[J].Carbohydrate Polymers,2012,90(4):1664-1670.
[14]董晓静.波纹巴非蛤糖胺聚糖抗氧化与降血脂活性研究[D].湛江:广东海洋大学,2010.
[15]OYAIZU M.Studies on product of browning reaction prepared from glucose amine[J].Japanese journal of Nutrition,1986,44(6):307-315.
[16]刘义,钱和.糖胺聚糖的提取、分离方法[J].海洋水产研究,2005,26(4):89-93.
[17]QIAO D L,KE C L,HU B,et al.Antioxidant activities of polysaccharides from Hyriopsis cumingii[J].Carbohydrate Polymers,2009,78(2):199-204.
[18]罗晓航.PEF结合酶法提取鲍鱼脏器粗多糖及其抗氧化活性研究[D].福州:福建农林大学,2012.
[19]孙丽平,鲍长俊,柳建华,等.乌鳢分泌多糖和黏多糖的提取及其抗氧化活性研究[J].食品与发酵工业,2015,41(7):81-85.
[20]孙晓朋.四角蛤蜊糖胺聚糖的提取、纯化及生理活性研究[D].天津:天津科技大学,2011.
[21]许桂芹.福寿螺多糖的分离纯化及其生物活性研究[D].福州:福建农林大学,2008.