波形蛋白在卵巢子宫内膜样腺癌组织中的表达及临床意义
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摘要
目的探讨波形蛋白在卵巢子宫内膜样腺癌(OEA)组织中的表达及临床意义。方法采用免疫组化技术检测40例OEA组织、10例卵巢交界性子宫内膜样肿瘤组织、30例卵巢子宫内膜异位症组织以及30例正常卵巢组织中波形蛋白的表达情况。比较4种卵巢组织以及不同临床病理特征OEA患者的波形蛋白阳性表达率。结果波形蛋白在正常卵巢、卵巢子宫内膜异位症、卵巢交界性子宫内膜样肿瘤和OEA组织中的阳性率依次升高(P <0. 05)。临床分期Ⅲ～Ⅳ期者、伴淋巴结或远处转移者癌组织的波形蛋白阳性表达率分别高于Ⅰ～Ⅱ期者、无淋巴结或远处转移者(均P <0. 05)。结论波形蛋白在OEA组织中呈高表达,其可能与OEA的发生发展相关,并参与OEA的侵袭、转移过程。
Objective To investigate the expression and clinical significance of vimentin in the ovarian endometrioid adenocarcinoma( OEA) tissues. Methods Immunohistochemistry was performed to detect the expressions of vimentin in 40 cases of OEA tissues,11 cases of ovarian borderline endometrioid cancer tissues,30 cases of ovarian endometriosis tissues,and 30 cases of normal ovarian tissues. The positive expression rate of vimentin was compared among these 4 types of ovary tissues,and among patients with different clinicopathologic features. Results The positive expression rate of vimentin increased in the order of normal ovarian tissues,ovarian endometriosis tissues,ovarian borderline endometrioid cancer tissues and OEA tissues( P < 0. 05). The positive expression rates of vimentin were higher in the cases with clinical grade Ⅲ-Ⅳ and with lymph node/distant metastasis than in the cases with gradeⅠ-Ⅱ and without lymph node/distant metastasis,respectively( all P < 0. 05). Conclusion Vimentin is highly expressed in the OEA tissues,and it might be related to the occurrence and development of OEA and participate into the invasion and metastasis of OEA.
Objective To investigate the expression and clinical significance of vimentin in the ovarian endometrioid adenocarcinoma( OEA) tissues. Methods Immunohistochemistry was performed to detect the expressions of vimentin in 40 cases of OEA tissues,11 cases of ovarian borderline endometrioid cancer tissues,30 cases of ovarian endometriosis tissues,and 30 cases of normal ovarian tissues. The positive expression rate of vimentin was compared among these 4 types of ovary tissues,and among patients with different clinicopathologic features. Results The positive expression rate of vimentin increased in the order of normal ovarian tissues,ovarian endometriosis tissues,ovarian borderline endometrioid cancer tissues and OEA tissues( P < 0. 05). The positive expression rates of vimentin were higher in the cases with clinical grade Ⅲ-Ⅳ and with lymph node/distant metastasis than in the cases with gradeⅠ-Ⅱ and without lymph node/distant metastasis,respectively( all P < 0. 05). Conclusion Vimentin is highly expressed in the OEA tissues,and it might be related to the occurrence and development of OEA and participate into the invasion and metastasis of OEA.
引文
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[2]Zhao Y,Wang S,Qu YM,et al.Prognostic analysis for Chinese patients with stageⅠovarian endometrioid carcinoma[J].J Ovarian Res,2017,10(1):63.
[3]Ge P,Wei L,Zhang M,et al.TRPC1/3/6 inhibition attenuates the TGF-β1-induced epithelial-mesenchymal transition in gastric cancer via the Ras/Raf1/ERK signaling pathway[J].Cell Biol Int,2018,42(8):975-984.
[4]Ji DG,Guan LY,Luo X,et al.Inhibition of MALAT1 sensitizes liver cancer cells to 5-flurouracil by regulating apoptosis through IKKαNF-γB pathway[J].Biochem Biophys Res Commun,2018,501(1):33-40.
[5]Kai K,Iwamoto T,Zhang D,et al.CSF-1/CSF-1R axis is associated with epithelial/mesenchymal hybrid phenotype in epithelial-like inflammatory breast cancer[J].Sci Rep,2018,8(1):9 427.
[6]Duan L,Ye L,Zhuang L,et al.VEGFC/VEGFR3 axis mediates TGFβ1-induced epithelial-to-mesenchymal transition in non-small cell lung cancer cells[J].PLo S One,2018,13(7):e0200452.
[7]刘春花,江庆萍,林丹,等.子宫内膜癌中丝裂原活化蛋白激酶4和波形蛋白的表达及临床意义[J].南方医科大学学报,2017,32(2):157-164.
[8]Li SS,Xu LZ,Zhou W,et al.p62/SQSTM1 interacts with vimentin to enhance breast cancer metastasis[J].Carcinogenesis,2017,38(11):1 092-1 103.
[9]Zhu Y,Zhang Y,Sui Z,et al.USP14 de-ubiquitinates vimentin and miR-320a modulates USP14 and vimentin to contribute to malignancy in gastric cancer cells[J].Oncotarget,2017,8(30):48 725-48 736.
[10]Jin H,Zhao Y,Zhang S,et al.Hyperthermia inhibits the motility of gemcitabine-resistant pancreatic cancer PANC-1cells through the inhibition of epithelial-mesenchymal transition[J].Mol Med Rep,2018,17(5):7 274-7 280.
[11]Tadokoro A,Kanaji N,Liu D,et al.Vimentin regulates invasiveness and is a poor prognostic marker in non-small cell lung cancer[J].Anticancer Res,2016,36(4):1 545-1 551.
[12]Lin H,Huang B,Wang H,et al.MTHFD2 overexpression predicts poor prognosis in renal cell carcinoma and is associated with cell proliferation and vimentin-modulated migration and invasion[J].Cell Physiol Biochem,2018,51(2):991-1 000.
[13]Niknami Z,Eslamifar A,Emamirazavi A,et al.The association of vimentin and fibronectin gene expression with epithelial-mesenchymal transition and tumor malignancy in colorectal carcinoma.[J].EXCLI J,2017,16:1 009-1 017.
[14]Li X,Yang J,Wang X,et al.Role of TWIST2,E-cadherin and Vimentin in epithelial ovarian carcinogenesis and prognosis and their interaction in cancer progression[J].Eur JGynaecol Oncol,2016,37(1):100-108.
[15]周丹丹.ZEB1、E-cadherin和Vimentin在上皮性卵巢癌中的表达及相关性研究[D].苏州:苏州大学,2015.
[16]Kenda Suster N,Smrkolj S,Virant-Klun I.Putative stem cells and epithelial-mesenchymal transition revealed in sections of ovarian tumor in patients with serous ovarian carcinoma using immunohistochemistry for vimentin and pluripotency-related markers[J].J Ovarian Res,2017,10(1):11.
[17]Davidson B,Holth A,Hellesylt E,et al.The clinical role of epithelial-mesenchymal transition and stem cell markers in advanced-stage ovarian serous carcinoma effusions[J].Hum Pathol,2015,46(1):1-8.
[18]Huang HN,Lin MC,Tseng LH,et al.Ovarian and endometrial endometrioid adenocarcinomas have distinct profiles of microsatellite instability,PTEN expression,and ARID1Aexpression[J].Histopathology,2015,66(4):517-528.
[19]任芳,王丹波.卵巢子宫内膜样腺癌中富含半胱氨酸的酸性分泌蛋白/骨连接素2与基质金属蛋白酶2表达及相关性研究[J].中国实用妇科与产科杂志,2015,31(12):1 125-1 128.
[20]Desouki MM,Kallas SJ,Khabele D,et al.Differential vimentin expression in ovarian and uterine corpus endometrioid adenocarcinomas:diagnostic utility in distinguishing double primaries from metastatic tumors[J].Int J Gynecol Pathol,2014,33(3):274-281.