光合细菌PSB06浸种对水稻促生作用研究
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摘要
光合细菌作为一种微生物肥料和菌剂,在农业生产应用上越来越广泛。探讨光合细菌对植物的促生作用将为光合细菌的进一步应用推广提供理论依据。使用光合细菌中的沼泽红假单胞菌PSB06菌剂对水稻种子浸种,结果表明,光合细菌PSB06菌剂处理能促进种子萌发。与对照(蒸馏水浸种)相比,其种子发芽率提高5个百分点,萌发种子的芽长和根长分别增加250%和249%;同时,光合细菌处理也能促进苗期生长。水稻茎秆比对照高36.0%,叶片体内叶绿素a、b含量分别提高27.5%和18.0%。在干旱胁迫下,光合细菌浸种过的苗期水稻SOD值和POD值比对照分别提高72.0%和38.5%,能更有效维持植物体内自由基的平衡。因此,光合细菌PSB06浸种可提高种子活力、促进苗期生长和增强耐胁迫能力,是一种高效的浸种剂,对水稻具有良好促生作用。
As a microbial fertilizer and bacterial inoculant, photosynthetic bacteria have been more and more widely used in agriculture. Clarifying the effects of photosynthetic bacteria on growth-promoting can provide theoretical basis for further practical application. The research on soaking rice seed with the Rhodopseudomonas plurties PSB06 inoculant in photosythetic bacteria was conducted. The results showed that the PSB06 inoculant could enhance seed germination. Compared with the control(distilled water soaking), it increased seed germination rate by five percentage points, bud length by 250% and root length by 249%. The PSB06 inoculant could also promote the growth of seedlings. The plant height of seedling were 36% higher, Chlorophyll a content 27.5% higher and Chlorophyll b content 18.0% higher than the control. Under the drought stress conditions, when treated with PSB06 inoculant, the superoxide dismutase(SOD) content and the peroxidase(POD) content in leaf increased by 72.0% and 38.5%, respectively, compared with the control, indicating that the treatment of PSB06 inoculant could effectively keep the balance in the contents of free radicals in plant body. Therefore, the PSB06 inoculant of photosythetic bacteria, which can increase seed vitality, promote seedling growth and improve tolerance to drought stress, is an efficient seed-soaking agent and has a good promoting effect on rice growth.
As a microbial fertilizer and bacterial inoculant, photosynthetic bacteria have been more and more widely used in agriculture. Clarifying the effects of photosynthetic bacteria on growth-promoting can provide theoretical basis for further practical application. The research on soaking rice seed with the Rhodopseudomonas plurties PSB06 inoculant in photosythetic bacteria was conducted. The results showed that the PSB06 inoculant could enhance seed germination. Compared with the control(distilled water soaking), it increased seed germination rate by five percentage points, bud length by 250% and root length by 249%. The PSB06 inoculant could also promote the growth of seedlings. The plant height of seedling were 36% higher, Chlorophyll a content 27.5% higher and Chlorophyll b content 18.0% higher than the control. Under the drought stress conditions, when treated with PSB06 inoculant, the superoxide dismutase(SOD) content and the peroxidase(POD) content in leaf increased by 72.0% and 38.5%, respectively, compared with the control, indicating that the treatment of PSB06 inoculant could effectively keep the balance in the contents of free radicals in plant body. Therefore, the PSB06 inoculant of photosythetic bacteria, which can increase seed vitality, promote seedling growth and improve tolerance to drought stress, is an efficient seed-soaking agent and has a good promoting effect on rice growth.
引文
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[27]Wei H,Okunishi S,Yoshikawa T,et al.Synergistic effect of photosynthetic bacteria and isolated bacteria in their antifungal activities against root rot fungi[J].Biocontrol Science,2016,21(3):173-177.
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[29]谢春琼,魏兰芳,汪钱龙.植物促生细菌对辣椒生长的影响[J].贵州农业科学,2013,41(2):124-126.
[30]任红,罗丰,许彦,等.菜心叶绿素测定方法比较研究[J].安徽农业科学,2012,40(3):1 455-1 456.
[31]刘晶,张鹤婷,殷悦,等.外源硫化氢对干旱胁迫下萌发水稻种子抗氧化代谢的影响[J].南方农业学报,2017,48(1):31-37.
[32]王兰,赛千蕙.低温胁迫对水稻苗期SOD、POD活性的影响[J].湖南农业科学,2011(11):56-58.
[33]徐刚,王彩莲,赵孔南,等.培养基和培养时间对水稻成熟和胚培养力的影响[J].种子,1990(04):21-24.
[2]Olson J M.Chlorophyll organization and function in green photosynthetic bacteria[J].Photochemistry&Photobiology,2010,67(1):61-75.
[3]Idi A,Nor M H M,Wahab M F A,et al.Photosynthetic bacteria:an eco-friendly and cheap tool for bioremediation[J].Reviews in Environmental Science&Biotechnology,2015,14(2):271-285.
[4]Hallenbeck P C,Liu Y.Recent advances in hydrogen production by photosynthetic bacteria[J].International Journal of Hydrogen Energy,2016,41(7):4 446-4 454.
[5]Lu H,Zhang G,Dai X,et al.Comparing three methods for photosynthetic bacteria separation and recycling during wastewater treatment[J].Desalination&Water Treatment,2016,57(27):12 467-12 477.
[6]张全国,王毅.光合细菌生物制氢技术研究进展[J].农业机械学报,2013,44(06):156-161.
[7]Lu H,Zhang G,Lu Y,et al.Using co-metabolism to accelerate synthetic starch wastewater degradation and nutrient recovery in photosynthetic bacterial wastewater treatment technology[J].Environmental Technology,2016,37(7):775.
[8]Zhou Q,Zhang P,Zhang G,et al.Biomass and pigments production in photosynthetic bacteria wastewater treatment:Effects of photoperiod[J].Bioresource Technology,2015,179(179C):505.
[9]Hosseini S S,Aghbashlo M,Tabatabaei M,et al.Exergy analysis of biohydrogen production from various carbon sources via anaerobic photosynthetic bacteria(Rhodospirillum rubrum)[J].Energy,2015,93:730-739.
[10]付保荣,曹向宇,冷阳,等.光合细菌对水产养殖水质和水生生物的影响[J].生态科学,2008(02):102-106.
[11]黄雪娇,杨冲,罗雅雪,等.光合细菌在水污染治理中的研究进展[J].中国生物工程杂志,2014,34(11):119-124.
[12]丁洪玲,张庆宇,黄初女,等.光合细菌对大豆的根瘤及产量的影响[J].大豆科技,2016(05):9-13.
[13]杨芳,田俊岭,杨盼盼,等.高效光合细菌菌剂对番茄品质、土壤肥力及微生物特性的影响[J].华南农业大学学报,2014(1):49-54.
[14]张信娣,曹慧,徐冬青,等.光合细菌和有机肥对土壤主要微生物类群和土壤酶活性的影响[J].土壤,2008,(03):443-447.
[15]刘勇,张德咏,王小平.光合细菌PSB-1对辣椒苗期生长和产量的影响[J].湖南农业科学,2000(5):19-20.
[16]胡青平,卫红萍,高红,等.光合细菌PSB-B浸种对小麦种子萌发的影响[J].麦类作物学报,2011,31(04):720-723.
[17]王秋菊,焦峰,崔战利,等.光合细菌浸种对水稻种子萌发的影响[J].作物杂志,2012(03):126-129.
[18]赵志平,聂鑫,丁杰,等.光合细菌研究进展[J].四川理工学院学报(自科版),2012,25(5):1-5.
[19]王秋菊.光合细菌对苗期水稻抗氧化作用的影响[J].黑龙江农业科学,2009(06):33-35.
[20]戴建平,程菊娥,刘勇,等.光合细菌PSB07-15对辣椒及土壤中甲氰菊酯残留的生物修复[J].生态环境学报,2010,19(10):2 441-2 444.
[21]崔中利,崔利霞,黄彦,等.农药污染微生物降解研究及应用进展[J].南京农业大学学报,2012,35(5):93-102.
[22]Daniel G,Cecile B.Method of degrading TBP using a photosynthetic bacterial strain:EP,US 20080145917 A1[P].2015.
[23]陈莎.光合细菌cs-1对稻曲病菌拮抗活性的测定[D].长沙:中南大学,2012.
[24]成飞雪,王忠勇,刘勇.光合细菌与芽孢杆菌生防菌剂防治黄瓜根结线虫病研究[J].长江蔬菜,2012(20):80-83.
[25]Su P,Tan X,Li C,et al.Photosynthetic bacterium Rhodopseudomonas palustris GJ-22 induces systemic resistance against viruses[J].Microbial Biotechnology,2017,10(3):612-624.
[26]罗源华,陈冰,张卓,等.光合细菌对辣椒疫病的田间防治试验[J].南方农业学报,2013,44(10):1 658-1 661.
[27]Wei H,Okunishi S,Yoshikawa T,et al.Synergistic effect of photosynthetic bacteria and isolated bacteria in their antifungal activities against root rot fungi[J].Biocontrol Science,2016,21(3):173-177.
[28]赵蕾,滕安娜.木霉对植物的促生及诱导抗性研究进展[J].植物保护,2010,36(3):43-46.
[29]谢春琼,魏兰芳,汪钱龙.植物促生细菌对辣椒生长的影响[J].贵州农业科学,2013,41(2):124-126.
[30]任红,罗丰,许彦,等.菜心叶绿素测定方法比较研究[J].安徽农业科学,2012,40(3):1 455-1 456.
[31]刘晶,张鹤婷,殷悦,等.外源硫化氢对干旱胁迫下萌发水稻种子抗氧化代谢的影响[J].南方农业学报,2017,48(1):31-37.
[32]王兰,赛千蕙.低温胁迫对水稻苗期SOD、POD活性的影响[J].湖南农业科学,2011(11):56-58.
[33]徐刚,王彩莲,赵孔南,等.培养基和培养时间对水稻成熟和胚培养力的影响[J].种子,1990(04):21-24.