高血压合并Hp感染者游离脂肪酸和氧化应激水平相关性分析
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摘要
目的观察原发性高血压(essential hypertension,EH)合并幽门螺旋杆菌(helicobacter pylori,Hp)感染者游离脂肪酸(free fatty acid,FFA)和氧化应激水平,分析其相关性。方法选择2017-01-01-2018-06-30利津县第二人民医院临床确诊的EH患者180例及对照组60例,检测14 C尿素呼气试验(14 C-UBT),采用酶联法检测FFA水平,采用比色法检测血清丙二醛(superoxide dismutase,SOD)活性,采用TBA法检测血清丙二醛(malondialdehyde,MDA)水平。结果EH组血清FFA水平为(0.79±0.20)mmol/L,高于对照组的(0.45±0.12)mmol/L,t=16.957,P<0.001;EH组血清MDA水平为(11.02±2.35)nmol/mL,高于对照组的(4.79±0.99)nmol/mL,t=6.168,P<0.001;EH组血清SOD水平为(92.75±8.92)U/mL,低于对照组的(131.73±10.06)U/mL,t=12.993,P<0.001。EH患者Hp感染组血清FFA水平为(0.97±0.19)mmol/L,高于Hp非感染组的(0.65±0.17)mmol/L,t=9.512,P<0.001;MDA水平为(14.16±2.31)nmol/mL,高于Hp非感染组的(8.62±1.94)nmol/mL,t=4.617,P<0.001;SOD水平为(77.55±8.05)U/mL,低于Hp非感染组的(104.37±8.79)U/mL,t=8.652,P<0.001。EH合并Hp感染者,血清FFA水平分别与血清MDA水平呈正相关,r=0.501,P<0.01;FFA水平与血清SOD活性呈负相关,r=-0.443,P<0.01。结论EH合并Hp感染者机体内游离脂肪酸水平升高及氧化应激反应增强,血清游离脂肪酸水平与氧化应激水平相关。
OBJECTIVE To explore the levels of free fatty acids(FFA)and oxidative stress in patients with essential hypertension(EH)complicated with helicobacter pylori infection,and to analyze their correlation.METHODS A total of 180 patients with clinical diagnosis of EH and the control group with 60 cases were selected.14 C urea breath test(14 C-UBT)was performed,the FFA levels,serum superoxide dismutase(SOD)activity,and the serum malondialdehyde(MDA)level were detected with enzyme-linked method,colorimetry and TBA method,respectively.RESULTS The serum FFA(0.79±0.20)mmol/L and MDA levels(11.02±2.35)nmol/ml of EH group were higher than those of control group(0.45±0.12)mmol/L,(4.79±0.99)nmol/ml.The SOD activity of EH group was(92.75±8.92)U/ml,lower than that of the control group(131.73±10.06)U/ml.The differences were all statistically significant(t=16.957,6.168,12.993,all P<0.001).The serum FFA and MDA levels,the SOD activity of Hpinfection group and Hpnon-infection group in EH patients were compared[(0.97±0.19)mmol/Lvs(0.65±0.17)mmol/L,(14.16±2.31)nmol/mL vs(8.62±1.94)nmol/mL,(77.55±8.05)U/mL vs(104.37±8.79)U/mL],and the differences were statistically significant(t=9.512,4.617,8.652,all P<0.001).Serum FFA level in EH patients with Hpinfection showed a significant positive correlation with serum MDA level(r=0.501,P<0.01),and a significant negative correlation with serum SOD activity(r=-0.443,P<0.01).CONCLUSIONS EH combined Hpinfection significantly increase the level of FFA in body and enhance oxidative stress.And oxidative stress is significantly related to the level of serum FFA.
OBJECTIVE To explore the levels of free fatty acids(FFA)and oxidative stress in patients with essential hypertension(EH)complicated with helicobacter pylori infection,and to analyze their correlation.METHODS A total of 180 patients with clinical diagnosis of EH and the control group with 60 cases were selected.14 C urea breath test(14 C-UBT)was performed,the FFA levels,serum superoxide dismutase(SOD)activity,and the serum malondialdehyde(MDA)level were detected with enzyme-linked method,colorimetry and TBA method,respectively.RESULTS The serum FFA(0.79±0.20)mmol/L and MDA levels(11.02±2.35)nmol/ml of EH group were higher than those of control group(0.45±0.12)mmol/L,(4.79±0.99)nmol/ml.The SOD activity of EH group was(92.75±8.92)U/ml,lower than that of the control group(131.73±10.06)U/ml.The differences were all statistically significant(t=16.957,6.168,12.993,all P<0.001).The serum FFA and MDA levels,the SOD activity of Hpinfection group and Hpnon-infection group in EH patients were compared[(0.97±0.19)mmol/Lvs(0.65±0.17)mmol/L,(14.16±2.31)nmol/mL vs(8.62±1.94)nmol/mL,(77.55±8.05)U/mL vs(104.37±8.79)U/mL],and the differences were statistically significant(t=9.512,4.617,8.652,all P<0.001).Serum FFA level in EH patients with Hpinfection showed a significant positive correlation with serum MDA level(r=0.501,P<0.01),and a significant negative correlation with serum SOD activity(r=-0.443,P<0.01).CONCLUSIONS EH combined Hpinfection significantly increase the level of FFA in body and enhance oxidative stress.And oxidative stress is significantly related to the level of serum FFA.
引文
[1]韩秀英,杜咏梅,付金国.老年原发性高血压患者血清脂联素水平与胰岛素抵抗的相关性研究[J].医学临床研究,2016,33(7):1405-1406,1407.
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[13]陈效琴,任静.游离脂肪酸、胰岛素抵抗与代谢综合征相关因素的关系[J].检验医学,2016,31(7):553-558.
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[16]李旭升,郭长城.幽门螺杆菌感染对血脂、血清炎症因子的影响及其与冠心病的相关性[J].实验与检验医学,2017,35(6):940-942.
[17]郝志华,陈春彦,王俊明,等.糖尿病患者幽门螺杆菌感染情况及颈动脉粥样硬化相关因素研究[J].中国全科医学,2012,15(17):1914-1916.
[18]张同庆,杨文东.急性脑梗死并发幽门螺旋杆菌感染者血脂水平及氧化应激反应的观察[J].现代检验医学杂志,2018,33(2):66-69.
[19]胡彩虹,韦锦涛,郭东亮.游离脂肪酸检测在2型糖尿病胰岛素抵抗临床应用[J].糖尿病新世界,2017,20(4):61-62.
[2]杨文东.游离脂肪酸与妊娠期糖尿病胰岛素抵抗的关系[J].现代检验医学杂志,2013,28(1):124-126.
[3]许银银,刘北忠.血清游离脂肪酸、同型半胱氨酸与代谢综合征的相关性研究[J].检验医学与临床,2015,12(21):3131-3132,3136.
[4]韩秀敏,杨文东.原发性高血压并发颈动脉粥样硬化患者血清同型半胱氨酸水平与氧化应激的关系[J].中国基层医药,2017,17(13):1929-1932.
[5]陈凯,卢乐,蔡恩照,等.2型糖尿病患者幽门螺杆菌感染与胰岛素抵抗的关系[J].中国慢性病预防与控制,2016,24(11):851-853.
[6]安振国,杨文东.2型糖尿病合并冠心病与幽门螺杆菌感染的相关性研究[J].国际医药卫生导报,2014,20(18):2872-2875.
[7]杨文东.原发性高血压患者颈动脉粥样硬化与幽门螺杆菌感染的相关性研究[J].中国医师杂志,2014,22(3):347-349,352.
[8]中国高血压防治指南修订委员会.中国高血压防治指南2010[J].中华高血压杂志,2011,19(8):701-743.
[9]周道平,贺小丹.幽门螺杆菌感染和胃食管反流病的关系探讨[J].实验与检验医学,2016,34(6):753-755.
[10]杜洁,杨维,张桂侠,等.幽门螺杆菌感染与血脂代谢及动脉粥样硬化的相关性[J].现代检验医学杂志,2017,32(4):104-106.
[11]杜洁,朱娜,李红艳,等.幽门螺杆菌感染对慢性阻塞性肺疾病血气分析及肺功能的影响[J].现代检验医学杂志,2017,32(6):99-101.
[12]朱文奎,徐绮,陈晋.血清钙、游离脂肪酸与社区老年代谢综合征的相关性分析[J].检验医学,2018,33(5):407-410.
[13]陈效琴,任静.游离脂肪酸、胰岛素抵抗与代谢综合征相关因素的关系[J].检验医学,2016,31(7):553-558.
[14]Saxena R,Kaur J.Th1/Th2cytokines and their genotypes as predictors ofhepatitis B virus related hepatocellular carcinoma[J].World JHepatol,2015,7(11):1572-1580.
[15]Verit A,Yuksel OH,Kivrak M,et al.Are Helicoba-cter pylori and benign prostatic hyperplasia related,and if so,how?[J].Urol J,2015,12(4):2271-2275.
[16]李旭升,郭长城.幽门螺杆菌感染对血脂、血清炎症因子的影响及其与冠心病的相关性[J].实验与检验医学,2017,35(6):940-942.
[17]郝志华,陈春彦,王俊明,等.糖尿病患者幽门螺杆菌感染情况及颈动脉粥样硬化相关因素研究[J].中国全科医学,2012,15(17):1914-1916.
[18]张同庆,杨文东.急性脑梗死并发幽门螺旋杆菌感染者血脂水平及氧化应激反应的观察[J].现代检验医学杂志,2018,33(2):66-69.
[19]胡彩虹,韦锦涛,郭东亮.游离脂肪酸检测在2型糖尿病胰岛素抵抗临床应用[J].糖尿病新世界,2017,20(4):61-62.