九香虫血淋巴三种生物分子对胃癌SGC-7901细胞增殖影响
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摘要
现代医学证明九香虫具有良好的抑癌抗癌作用。为了明确九香虫抑癌抗癌活性组份大类,采用超滤技术分离九香虫血淋巴小分子物质、60%丙酮沉淀蛋白质、醇提法提取多糖成分,通过MTT法检测三种生物分子对SGC-7901细胞的增殖影响。结果表明,在较高的作用浓度条件下,蛋白质提取物的增殖抑制活性显著高于小分子物质(P<0.05),多糖提取物各作用浓度与无药对照相比均无明显的抑制活性,说明九香虫蛋白质组份是其抑癌抗癌的主要功能分子。
Modern medicine has proved that Aspongopuschinensis chinensis has obvious anticancer effects. In order to clarify the active components of A. chinensis,the ultrafiltration technique was employed to separate small molecules of hemolymph,60% acetone was used to precipitate proteins,and alcohol extraction method was carried out to extract polysaccharides. The proliferation of SGC-7901 cells was detected by MTT assay.The inhibitory activity of the protein extract was significantly higher than that of the small molecules( P <0. 05) under the higher concentration conditions. The polysaccharide extract had no obvious inhibitory activity compared with no drug control. The results showed that the protein extract of A. chinensis is the main anticancer molecules.
Modern medicine has proved that Aspongopuschinensis chinensis has obvious anticancer effects. In order to clarify the active components of A. chinensis,the ultrafiltration technique was employed to separate small molecules of hemolymph,60% acetone was used to precipitate proteins,and alcohol extraction method was carried out to extract polysaccharides. The proliferation of SGC-7901 cells was detected by MTT assay.The inhibitory activity of the protein extract was significantly higher than that of the small molecules( P <0. 05) under the higher concentration conditions. The polysaccharide extract had no obvious inhibitory activity compared with no drug control. The results showed that the protein extract of A. chinensis is the main anticancer molecules.
引文
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[15]Gajski G,Garajvrhovac V.Melittin:a lytic peptide with anticancer properties[J].Environmental Toxicology Pharmacology,2013,36(2):697-705.
[2]Khazir J,Riley DL,Pilcher LA,et al.Anticancer agents from diverse natural sources[J].Natural Product Communications,2014,9(11):1655-1669.
[3]Feng Y,Zhao M,Zhao HE,et al.Research and utilization of medicinal insects in China[J].Entomological Research,2010,39(5):313-316.
[4]彩万志,庞雄飞,花保祯,等.普通昆虫学[M](2版).北京:中国农业大学出版社,2011.
[5]Seabrooks L,Hu L.Insects:an underrepresented resource for the discovery of biologically active natural products[J].Acta Pharmaceutica Sinica,2017,7(4):409-426.
[6]高源,陈建伟,李鹏,等.九香虫抗凝血作用的研究[J].现代中药研究与实践,2010,24(03):34-36.
[7]檀军,郭建军,魏超,等.九香虫血淋巴对胃癌SGC-7901细胞体外增殖的抑制作用[J].山地农业生物学报,2013,32(02):119-122.
[8]Ratcliffe NA,Mello CB,Garcia ES,et al.Insect natural products and processes:new treatments for human disease[J].Insect Biochemistry Molecular Biology,2011,41(10):747-769.
[9]李文宾,吴敏丽,廉振民,等.中国昆虫资源研究开发现状[J].氨基酸和生物资源,2008(04):21-25.
[10]Jiang,SJ.Anti-cancer insect medicinal materials in China[J].Chin.Med.Mater,1990(13):11-14.
[11]潘大理.九香虫治愈血管瘤4例[J].中医杂志,1987,28(11):40.
[12]林普莲.九香抗癌定痛散治癌痛23例观察[J].福建中医学院学报,2005(S1):123-124.
[13]张卫东,赵惠儒,阎影,等.斑蝥素诱导人肺癌A549细胞凋亡及其分子机制的研究[J].中华肿瘤杂志,2005(06):330-334.
[14]李晓飞,侯晓晖,陈祥盛.芫菁斑蝥素对喉癌细胞和胃癌细胞的抑制作用[J].昆虫学报,2009,52(09):946-951.
[15]Gajski G,Garajvrhovac V.Melittin:a lytic peptide with anticancer properties[J].Environmental Toxicology Pharmacology,2013,36(2):697-705.