聚磺酸甜菜碱功能化金纳米粒子的抗蛋白吸附
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摘要
针对金纳米粒子(GNPs)在体内吸附蛋白质从而引起生物相容性的问题,通过两性离子聚合物表面修饰来提高金纳米粒子的抗蛋白吸附性能。通过可逆加成断裂链转移聚合(RAFT)制备了聚磺酸甜菜碱(PSBMA),并利用巯基与金之间的强耦合作用获得了PSBMA修饰的金纳米粒子PSBMA-@-GNPs。利用UVvis光谱、动态光散射(DLS)和透射电镜(TEM)证实了PSBMA-@-GNPs的核-壳纳米结构。以牛血清白蛋白(BSA)为蛋白模型,对比了GNPs、PEG-@-GNPs和PSBMA-@-GNPs对BSA的吸附性能。研究结果表明:由于表面PSBMA特殊的聚合物长链和两性离子结构,大大降低了GNPs的蛋白吸附。
In order to solve the problem that protein-adsorbing capacities of gold nanoparticles( GNPs) caused the poor biocompatibility in vivo,anti fouling properties of GNPs were increased by surface coating of zwitterionic polymer. Poly-sulfobetaine methaerylate( PSBMA) was prepared by reverslable addtion fragmentation chain transfer pdymerization( RAFT),and then was coated on the surface of GNPs to prepare PSBMA-@-GNPs via the strong binding between the thiol end-functionalization of polymer and gold. The coreshell structure of PSBMA-@-GNPs was determined by UV-vis spectra,dynamic light scattering( DLS) and transmission electron microscopy( TEM). Taking bovine serum albumin( BSA) as a protein model,the protein absorption of GNPs,PEG-@-GNPs and PSBMA-@-GNPs was investigated. The results indicate that the protein absorption of GNPs is greatly reduced by PSBMA modification due to its long-chain structure and zwitterionic property.
In order to solve the problem that protein-adsorbing capacities of gold nanoparticles( GNPs) caused the poor biocompatibility in vivo,anti fouling properties of GNPs were increased by surface coating of zwitterionic polymer. Poly-sulfobetaine methaerylate( PSBMA) was prepared by reverslable addtion fragmentation chain transfer pdymerization( RAFT),and then was coated on the surface of GNPs to prepare PSBMA-@-GNPs via the strong binding between the thiol end-functionalization of polymer and gold. The coreshell structure of PSBMA-@-GNPs was determined by UV-vis spectra,dynamic light scattering( DLS) and transmission electron microscopy( TEM). Taking bovine serum albumin( BSA) as a protein model,the protein absorption of GNPs,PEG-@-GNPs and PSBMA-@-GNPs was investigated. The results indicate that the protein absorption of GNPs is greatly reduced by PSBMA modification due to its long-chain structure and zwitterionic property.
引文
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[3]LIU G Y,LUO Q Q,WANG H B,et al.In situ synthesis of multidentate PEGylated chitosan modified gold nanoparticles with good stability and biocompatibility[J].RSC advances,2015,5(86):70109-70116.
[4]吴疆.抗蛋白质非特异性吸附材料内在机理和特性的研究[D].杭州:浙江大学,2014.
[5]翟宏菊,戴晓威,曹爽,等.金纳米粒子的合成、性质及其应用新进展[J].吉林师范大学学报(自然科学版),2017,38(2):13-16.
[6]LI W,LIU Q,LIU L.Antifouling gold surfaces grafted with aspartic acid and glutamic acid based zwitterionic polymer brushes[J].Langmuir the ACS journal of surfaces&colloids,2014,30(42):12619.
[7]慈吉良,康宏亮,刘晨光,等.两性离子聚合物的抗蛋白质吸附机理及其应用[J].化学进展,2015,27(9):1198-1212.
[8]陈杨军,刘湘圣,王海波,等.生物医用纳米颗粒表面的两性离子化设计[J].化学进展,2014,26(11):1849-1858.
[9]魏秀珍,孔新,王松雪,等.两性离子在高分子膜改性及提高膜抗污染性中的研究进展[J].功能材料,2014,45(2):2007-2013.
[10]张倩,沈冲,孟琴.磺基甜菜碱接枝共聚物共混改性聚砜超滤膜的研究[J].高校化学工程学报,2014,28(6):1366-1371.
[11]张杨.两性离子材料改性芳香聚酰胺膜的制备及性能研究[D].杭州:浙江大学,2013.
[12]WANG Y T,LU X M,ZHU F,et al.The preparation of gold nanoparticles and evaluation of their immunological function effects on rats[J].Bio-medical materials and engineering,2014,24(1):885-892.
[13]VAISHANAV S K,CHANDRAKER K,KORRAM J,et al.Protein nanoparticle interaction:a spectrophotometric approach for adsorption kinetics and binding studies[J].Journal of molecular structure,2016,1117:300-310.
[14]李军波,韩晨,张世杰,等.聚甲基丙烯酸单层保护金纳米粒子选择性检测Pb2+[J].高等学校化学学报,2013,34(10):2303-2307.
[15]QIAO C M,LIU J D,YANG J,et al.Enhanced non-inflammasome mediated immune responses by mannosylated zwitterionic-based cationic liposomes for HIV DNA vaccines[J].Biomaterials,2016,85(4):1-17.