不同栽培方式银耳多糖单糖组成分析及体外抗氧化活性比较
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摘要
对代料和段木两种栽培方式的银耳子实体采用碱式提取其多糖,通过测定多糖的营养成分、单糖组成及体外抗氧化活性,比较两种栽培方式银耳品质的差别。结果表明,脱蛋白后段木和代料银耳多糖中蛋白质残留量分别2.65±0.05、0.45±0.06 mg/mL,两种银耳多糖的单糖都由甘露糖、葡萄糖、木糖和葡萄糖醛酸组成,代料银耳与段木银耳多糖中葡萄糖的质量比为13.62∶2.43。不同银耳多糖的体外抗氧化能力均小于维生素C,在两种银耳DPPH清除率趋于稳定时,段木银耳多糖的DPPH自由基清除率比代料银耳高3%,而代料银耳羟自由基清除率比段木银耳高0.25%。
The Tremella fuciformis mesocarps of the two cultivation methods of the substitute and the wood were extracted by basic extraction,and the difference of the properties of the two methods of Tremella fuciformis was compared by measuring the monosaccharide composition of the polysaccharide and the antioxidant activity in vitro. The results showed that the protein residues in the polysaccharides from Tremella fuciformis of wood and the substitute were 2.65±0.05 mg/mL and 0.45±0.06 mg/mL,respectively. The monosaccharides of both Tremella fuciformis polysaccharides were composed of mannose,glucose,xylose and glucuronic acid. And the mass ratio of glucose in the polysaccharides of two methods of Tremella fuciformis was 13.62∶2.43.It was showed that the anti-oxidation ability of different Tremella fuciformis polysaccharides was lower than that of vitamin C. When the DPPH scavenging rate of the two Tremella fuciformis tended to be stable,the DPPH free radical scavenging rate of the Tremella fuciformis polysaccharide was 3% higher than that of the substitute Tremella fuciformis. The rate of scavenging hydroxyl radicals of substituted Tremella fuciformis was 0.25% higher than that of the wood Tremella fuciformis.
The Tremella fuciformis mesocarps of the two cultivation methods of the substitute and the wood were extracted by basic extraction,and the difference of the properties of the two methods of Tremella fuciformis was compared by measuring the monosaccharide composition of the polysaccharide and the antioxidant activity in vitro. The results showed that the protein residues in the polysaccharides from Tremella fuciformis of wood and the substitute were 2.65±0.05 mg/mL and 0.45±0.06 mg/mL,respectively. The monosaccharides of both Tremella fuciformis polysaccharides were composed of mannose,glucose,xylose and glucuronic acid. And the mass ratio of glucose in the polysaccharides of two methods of Tremella fuciformis was 13.62∶2.43.It was showed that the anti-oxidation ability of different Tremella fuciformis polysaccharides was lower than that of vitamin C. When the DPPH scavenging rate of the two Tremella fuciformis tended to be stable,the DPPH free radical scavenging rate of the Tremella fuciformis polysaccharide was 3% higher than that of the substitute Tremella fuciformis. The rate of scavenging hydroxyl radicals of substituted Tremella fuciformis was 0.25% higher than that of the wood Tremella fuciformis.
引文
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[20]颜军,徐光域,郭晓强,等.银耳粗多糖的纯化及抗氧化活性研究[J].食品科学,2005(9):169-172.
[21]申明月,聂少平,谢明勇,等.茶叶多糖的糖醛酸含量测定及抗氧化活性研究[J].天然产物研究与开发,2007(5):830-833.
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[2]古小露,王冉冉,马嫄,等.基于营养成分和矿质元素含量判别银耳栽培方式[J].食品科技,2017,42(8):90-94.
[3]周永昌,汪昭月.银耳段木栽培[J].食用菌,1980(1):35-36.
[4]李清总.代料栽培银耳高产技术[J].食用菌,1993(2):26-27.
[5]马素云,贺亮,姚丽芬.银耳多糖结构与生物活性研究进展[J].食品科学,2010(23):411-416.
[6]江玉姬,孙淑静,官宁,等.银耳多糖提取工艺的研究[J]. 农业工程学报,2004(增1):262-263.
[7]吴琼,郑成,宁正祥,等.碱溶性银耳粗多糖的提取及其清除自由基作用的研究[J].食品科学,2007(6):153-155.
[8]吴振亚.银耳多糖的提取纯化、理化性质及抗氧化活性研究[D].雅安:四川农业大学,2015.
[9]曹桂华. 银耳多糖的制备、化学结构及活性研究[D]. 长春:长春中医药大学,2013.
[10]曾丽萍,梁钻好,杨子银,等.银耳多糖提取优化及得率与质构相关性[J].农业工程,2016(5):61-64,69.
[11]吴琼,代永刚,高长城,等.酸降解水溶性银耳多糖及抗氧化作用研究[J].食品科学,2009(13):93-96.
[12]姜瑞芝,陈英红,杨勇杰,等.银耳多糖中糖醛酸含量的测定[J].中草药,2004(9):36-38.
[13]招钰,周小玲,郭海燕,等.茶多糖中单糖组成比较[J].安徽农业大学学报,2007(4):547-550.
[14]郭晓强,何钢,姚倩,等.乙酰化银耳多糖的制备及其取代度测定[J].食品工业科技,2013(12):255-257.
[15]韩威,姜瑞芝,陈英红,等.银耳多糖单糖组成分析的三种色谱方法比较[J].天然产物研究与开发,2012(3):359-361,302.
[16]刘培勋,高小荣,徐文清,等.银耳碱提多糖抗氧化活性的研究[J].中国生化药物杂志,2005(3):169-170.
[17]Deng C,Sun Y,Fu H,et al. Antioxidant and immunostimulatory activities of polysaccharides extracted from Tremella aurantial bamycelia[J]. Molecular Medicine Reports,2016,14(5):4857.
[18]张泽生,孙东,徐梦莹,等.银耳多糖抗氧化作用的研究[J].食品研究与开发,2014(18):10-15.
[19]陈英红,姜翔之,罗浩铭,等.柱前衍生化高效液相色谱法分析银耳多糖的单糖组成[J].特产研究,2012(1):37-39,54.
[20]颜军,徐光域,郭晓强,等.银耳粗多糖的纯化及抗氧化活性研究[J].食品科学,2005(9):169-172.
[21]申明月,聂少平,谢明勇,等.茶叶多糖的糖醛酸含量测定及抗氧化活性研究[J].天然产物研究与开发,2007(5):830-833.
[22]王琪. 灵芝子实体多糖的分离纯化、硫酸化及生物活性研究[D].南京:南京农业大学,2010.