不同浓度下紫草提取物对子宫内膜异位症大鼠炎性因子、凋亡基因的影响
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摘要
目的探讨不同浓度下紫草提取物对子宫内膜异位症大鼠炎性因子、凋亡基因的影响。方法取SD大鼠40只,均采用腹腔注射苯甲酸雌二醇(0.1 mL/kg)方法制作子宫内膜异位症大鼠模型,将造模成功大鼠随机分为4组,每组10只。紫草提取物高浓度组每天灌胃紫草提取物5.6 g/kg,紫草提取物中浓度组每天灌胃紫草提取物2.8 g/kg,紫草提取物低浓度组每天灌胃紫草提取物1.4 g/kg,对照组则灌胃等量的生理盐水。4周后观察各组大鼠异位病灶组织匀浆中肿瘤坏死因子-α(TNF-α)、前列腺素E_2(PGE_2)、白细胞介素-6(IL-6)及凋亡基因Bcl-2和Bax表达情况。结果与对照组相比,紫草提取物各组大鼠TNF-α、PGE_2、IL-6及Bcl-2表达量均明显降低(P均<0.05),凋亡指数及Bax表达量均明显升高(P均<0.05),且紫草提取物高浓度组TNF-α、PGE_2、IL-6及Bcl-2表达量均明显低于紫草提取物低浓度组、紫草提取物中浓度组(P均<0.05),凋亡指数、Bax表达量均明显高于紫草提取物低浓度组、紫草提取物中浓度组(P均<0.05)。结论紫草提取物可降低子宫内膜异位症模型大鼠异位病灶内炎性因子水平,调节子宫内膜异位细胞凋亡基因表达量,且紫草提取物高浓度作用更好。
Objective It is to investigate the effects of lithospermum arborescens extracts(LAE) under different concentrations on the inflammatory and apoptosis genes in endometriosis rats. Methods Forty SD rats were selected and injected intraperitoneally with estradiol benzoate(0.1 mL/kg) to make rat models of endometriosis. The successful models were randomly divided into 4 groups, each group had 10 rats. The high concentration group of LAE was intragastrically administered 5.6 g/kg per day, the medium concentration group of LAE was intragastrically administered 2.8 g/kg per day, and the low concentration group of LAE was intragastrically administered 1.4 g/kg per day. The control group was given an equal amount of normal saline by gavage. After 4 weeks, the expressions of tumor necrosis factor(TNF-α), prostaglandin 2(PGE_2), interleukin-6(IL-6) and apoptosis genes Bcl-2 and Bax were observed in the ectopic lesions of rats in each group. Happening. Results Compared with the control group, the expressions of TNF-α, PGE_2, IL-6 and Bcl-2 in every LAE group were significantly decreased(P<0.05), and the apoptotic index and Bax expression were significantly increased(P<0.05), and the expression levels of TNF-α, PGE_2, IL-6 and Bcl-2 in the high concentration group of LAE were significantly lower than those in the low and medium concentration groups of LAE, the expressions of apoptotic index and Bax were significantly higher than those in the low and medium concentration groups of LAE(P<0.05). Conclusion LAE can reduce the level of inflammatory factors in ectopic lesions of endometriosis model rats and increase the expression of endometriotic cells, and the high concentration of LAE is better.
Objective It is to investigate the effects of lithospermum arborescens extracts(LAE) under different concentrations on the inflammatory and apoptosis genes in endometriosis rats. Methods Forty SD rats were selected and injected intraperitoneally with estradiol benzoate(0.1 mL/kg) to make rat models of endometriosis. The successful models were randomly divided into 4 groups, each group had 10 rats. The high concentration group of LAE was intragastrically administered 5.6 g/kg per day, the medium concentration group of LAE was intragastrically administered 2.8 g/kg per day, and the low concentration group of LAE was intragastrically administered 1.4 g/kg per day. The control group was given an equal amount of normal saline by gavage. After 4 weeks, the expressions of tumor necrosis factor(TNF-α), prostaglandin 2(PGE_2), interleukin-6(IL-6) and apoptosis genes Bcl-2 and Bax were observed in the ectopic lesions of rats in each group. Happening. Results Compared with the control group, the expressions of TNF-α, PGE_2, IL-6 and Bcl-2 in every LAE group were significantly decreased(P<0.05), and the apoptotic index and Bax expression were significantly increased(P<0.05), and the expression levels of TNF-α, PGE_2, IL-6 and Bcl-2 in the high concentration group of LAE were significantly lower than those in the low and medium concentration groups of LAE, the expressions of apoptotic index and Bax were significantly higher than those in the low and medium concentration groups of LAE(P<0.05). Conclusion LAE can reduce the level of inflammatory factors in ectopic lesions of endometriosis model rats and increase the expression of endometriotic cells, and the high concentration of LAE is better.
引文
[1] 詹志来,胡峻,刘谈. 紫草化学成分与药理活性研究进展[J]. 中国中药杂志,2015,40(21):4127-4134
[2] 袁冬平,史颖莉,顾林,等. 紫草素通过调控RANTES抑制子宫内膜异位症单核细胞募集[J]. 中国免疫学杂志,2010,26(7):619-623
[3] 赵海青,刘军锋,刘珂. 新疆紫草羟基萘醌类化学成分的研究及对PDE4的抑制作用[J]. 中国实验方剂学杂志,2010,16(10):96
[4] 毕景芹,薄国荣,赵华兴,等. 中西医结合治疗子宫内膜异位症临床研究[J]. 现代中西医结合杂志,2013,22(11):1162-1164
[5] 张玲玲,应小燕. 腹腔镜手术联合促性腺激素释放激素激动剂治疗子宫内膜异位症疗效的比较研究[J]. 中国微创外科杂志,2015,15(4):305-308
[6] 邓姗,郎景和,冷金花. 曼月乐对子宫内膜异位症患者在位内膜增殖与凋亡的影响[J]. 生殖与避孕,2006,26(10):589-601
[7] 夏晓梦,郭李璐,苏津平. 来曲唑对大鼠子宫内膜异位症的作用及对异位内膜细胞凋亡的影响[J]. 中南大学学报:医学版,2013,38(1):54-59
[8] Kyama CM,Mihalyi A,Simsa P,et al. Non-steroidal targets in the diagnosis and treatment of endometriosis[J]. Curr Med Chem,2008,15(10):1006-1017
[9] Boroujerdjerdi A,Welser-Alves JV,Milner R. Matrix metalloproei-nase-9 mediates post-hypoxic vascular pruning of cerebral blood vessels by degrading laminin and claud in-5[J]. Angiogenesis,2015,18(3):255-264
[10] 徐妍,何珏. 子宫内膜异位症模型大鼠接受米非司酮干预后炎性因子、侵袭和凋亡基因表达的评估[J]. 海南医学院学报,2017,23(9):1169-1174
[11] 代洪波,张艳. IL-6和TNF-α在子宫内膜异位症不孕患者血清中水平的变化及意义研究[J]. 中国性科学,2016,25(5):54-56
[12] 詹志来,胡峻,刘谈. 紫草化学成分与药理活性研究进展[J]. 中国中药杂志,2015,40(21):4127-4135
[13] 杨武斌,罗晓玲,胡娟. 紫草抗肿瘤活性成分及作用机制研究现状[J]. 解放军药学学报,2017,33(4):359-362
[14] 袁冬平,史颖莉,顾林. 紫草素抑制单核细胞募集及其对人鼠嵌合内异症模型的影响[J]. 生殖与避孕,2010,30(5):289-293
[15] 倪婕,顾林,史颖莉. 紫草素对人鼠嵌合内异位症模型血管内皮生长因子表达的影响[J]. 中国优生与遗传杂志,2011,19(1):56-57
[16] 张卓琦,曹希传,朱文玲. 紫草素抑制血管平滑肌细胞及巨噬细胞肿瘤坏死因子-α启动子活性[J]. 中国病理生理杂志,2007,23(7):1378-1381
[2] 袁冬平,史颖莉,顾林,等. 紫草素通过调控RANTES抑制子宫内膜异位症单核细胞募集[J]. 中国免疫学杂志,2010,26(7):619-623
[3] 赵海青,刘军锋,刘珂. 新疆紫草羟基萘醌类化学成分的研究及对PDE4的抑制作用[J]. 中国实验方剂学杂志,2010,16(10):96
[4] 毕景芹,薄国荣,赵华兴,等. 中西医结合治疗子宫内膜异位症临床研究[J]. 现代中西医结合杂志,2013,22(11):1162-1164
[5] 张玲玲,应小燕. 腹腔镜手术联合促性腺激素释放激素激动剂治疗子宫内膜异位症疗效的比较研究[J]. 中国微创外科杂志,2015,15(4):305-308
[6] 邓姗,郎景和,冷金花. 曼月乐对子宫内膜异位症患者在位内膜增殖与凋亡的影响[J]. 生殖与避孕,2006,26(10):589-601
[7] 夏晓梦,郭李璐,苏津平. 来曲唑对大鼠子宫内膜异位症的作用及对异位内膜细胞凋亡的影响[J]. 中南大学学报:医学版,2013,38(1):54-59
[8] Kyama CM,Mihalyi A,Simsa P,et al. Non-steroidal targets in the diagnosis and treatment of endometriosis[J]. Curr Med Chem,2008,15(10):1006-1017
[9] Boroujerdjerdi A,Welser-Alves JV,Milner R. Matrix metalloproei-nase-9 mediates post-hypoxic vascular pruning of cerebral blood vessels by degrading laminin and claud in-5[J]. Angiogenesis,2015,18(3):255-264
[10] 徐妍,何珏. 子宫内膜异位症模型大鼠接受米非司酮干预后炎性因子、侵袭和凋亡基因表达的评估[J]. 海南医学院学报,2017,23(9):1169-1174
[11] 代洪波,张艳. IL-6和TNF-α在子宫内膜异位症不孕患者血清中水平的变化及意义研究[J]. 中国性科学,2016,25(5):54-56
[12] 詹志来,胡峻,刘谈. 紫草化学成分与药理活性研究进展[J]. 中国中药杂志,2015,40(21):4127-4135
[13] 杨武斌,罗晓玲,胡娟. 紫草抗肿瘤活性成分及作用机制研究现状[J]. 解放军药学学报,2017,33(4):359-362
[14] 袁冬平,史颖莉,顾林. 紫草素抑制单核细胞募集及其对人鼠嵌合内异症模型的影响[J]. 生殖与避孕,2010,30(5):289-293
[15] 倪婕,顾林,史颖莉. 紫草素对人鼠嵌合内异位症模型血管内皮生长因子表达的影响[J]. 中国优生与遗传杂志,2011,19(1):56-57
[16] 张卓琦,曹希传,朱文玲. 紫草素抑制血管平滑肌细胞及巨噬细胞肿瘤坏死因子-α启动子活性[J]. 中国病理生理杂志,2007,23(7):1378-1381