钛纳米管表面固定GL13K抗菌肽的实验研究
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摘要
目的探讨在二氧化钛(TiO_2)纳米管表面固定GL13K抗菌肽(AMP)的方法并对其效果进行比较,为钛种植体表面的抗菌改性及种植体周围炎治疗研究提供新的思路。方法采用物理吸附、共价结合及层层自组装将GL13K抗菌肽固定至TiO_2纳米管表面,构建生物活性抗菌涂层,并比较其固定效果。结果通过场发射扫描电镜、正置荧光显微镜、接触角测量仪、X射线光电子能谱仪、傅里叶红外光谱以及紫外分光光度计对样品进行表征,证实3种方法均可将GL13K抗菌肽固定至TiO_2纳米管表面。结论层层自组装法的缓释效应最佳,有望提高钛种植体表面的持续抗菌性能。
Objective To investigate the method of immobilizing GL13 K antibacterial peptide( AMP) on the surface of titanium dioxide( Ti O2) nanotubes and compare the effects thereof. Finallly,offering new ideas for antimicrobial modification of titanium implant surface and the treatment of peri-implantitis. Methods The GL13 K AMP was immobileized on the Ti O2 nanotubes by physical adsorption,covalently bonding and layer-by-layer self-assembly to construct a bioactive antibacterial coating,and the fixation effect was compared. Results We characterized the s GL13 Kles by field emission scanning electron microscopy,fluorescence microscopy,contact angle measureing instrument,X-ray photoelectron spectroscopy,Fourier infrared spectroscopyand ultraviolet spectrophotometer. It was confirmed that all three methods can fix GL13 K AMP on the Ti O2 nanotubes. Conclusion Layer-by-layer selfassembly method has the best sustained release effect and it is expected to improve the sust-ained antibacterial properties of the titanium implant surface.
Objective To investigate the method of immobilizing GL13 K antibacterial peptide( AMP) on the surface of titanium dioxide( Ti O2) nanotubes and compare the effects thereof. Finallly,offering new ideas for antimicrobial modification of titanium implant surface and the treatment of peri-implantitis. Methods The GL13 K AMP was immobileized on the Ti O2 nanotubes by physical adsorption,covalently bonding and layer-by-layer self-assembly to construct a bioactive antibacterial coating,and the fixation effect was compared. Results We characterized the s GL13 Kles by field emission scanning electron microscopy,fluorescence microscopy,contact angle measureing instrument,X-ray photoelectron spectroscopy,Fourier infrared spectroscopyand ultraviolet spectrophotometer. It was confirmed that all three methods can fix GL13 K AMP on the Ti O2 nanotubes. Conclusion Layer-by-layer selfassembly method has the best sustained release effect and it is expected to improve the sust-ained antibacterial properties of the titanium implant surface.
引文
[1] Gallo J,Holinka M,Moucha C S. Antibacterial surface treatment for orthopaedic implants[J]. Int J Mol SCI,2014,15(8):13849-80.
[2] Li T,Wang N,Chen S,et al. Antibacterial activity and cytocompatibility of an implant coating consisting of Ti O2nanotubes combined with a GL13K antimicrobial peptide[J]. Int J Nanomedicine,2017,12:2995-3007.
[3] Bae I H,Yun K D,Kim H S,et al. Anodic oxidized nanotubular titanium implantsenhance bone morphogenetic protein-2 delivery[J]. J Biomed Mater Res B Appl Biomater,2010,93(2):484-91.
[4]柳江枫,胡天驹.抗菌肽的作用机制、生物活性及应用研究进展[J].微生物学免疫学进展,2012,40(5):84-8.
[5] Ohlsen K,Dandekar G,Schwarz R,et al. New trends in phar-macogenomic strategies against resistance development in microbial infections[J]. Pharmacogenomics,2008,9(11):1711-23.
[6] Gorr S U,Abdolhosseini M,Shelar A. Dual host-defence functions of SPLUNC2/PSP and synthetic peptides derived from the protein[J]. Biochem Soc Trans,2011,39(4):1028-32.
[7] Hirt H,Gorr S U. Antimicrobial peptide GL13K is effective in reducing biofilms of pseudomonas aeruginosa[J]. Antimicrob Agents Chemother,2013,57(10):4903-10.
[8] Bhardwaj G. Webster T J. Reduced bacterial growth and increased osteoblast proliferation on titanium with a nanophase Ti O2surface treatment[J]. International J Nanomedicine,2017,12:363-9.
[9] Guo Y P,Yao Y B,Guo Y J,et al. Hydrothermal fabrication of mesoporous carbonated hydroxyapatite microspheres for a drug delivery system[J]. Micropor Mesopor Mat,2012,155:245-51.
[10] Wang T,Weng Z,Liu X,et,al. Controlled release and biocompatibility of polymer/titania nanotube array system on titanium implants[J]. Bioact Mater,2017,2(1):44-50.
[11] Kazemzadeh-Narbat M,Lai B F,Ding C,et al. Multilayered coating on titanium for controlled release of antimicrobial peptides for the prevention of implant-associated infections[J]. Biomaterials,2013,34(24):5969-77.
[12]高啟坤,宋婷婷,吴齐越,等.钛纳米管表面固定成骨细胞特异性多肽的实验研究[J].安徽医科大学学报,2017,52(12):1790-94.
[13] Pawar S N,Edgar K J. Alginate derivatization:a review of chemistry,properties and applications[J]. Biomaterials,2012,33(11):3279-305.
[2] Li T,Wang N,Chen S,et al. Antibacterial activity and cytocompatibility of an implant coating consisting of Ti O2nanotubes combined with a GL13K antimicrobial peptide[J]. Int J Nanomedicine,2017,12:2995-3007.
[3] Bae I H,Yun K D,Kim H S,et al. Anodic oxidized nanotubular titanium implantsenhance bone morphogenetic protein-2 delivery[J]. J Biomed Mater Res B Appl Biomater,2010,93(2):484-91.
[4]柳江枫,胡天驹.抗菌肽的作用机制、生物活性及应用研究进展[J].微生物学免疫学进展,2012,40(5):84-8.
[5] Ohlsen K,Dandekar G,Schwarz R,et al. New trends in phar-macogenomic strategies against resistance development in microbial infections[J]. Pharmacogenomics,2008,9(11):1711-23.
[6] Gorr S U,Abdolhosseini M,Shelar A. Dual host-defence functions of SPLUNC2/PSP and synthetic peptides derived from the protein[J]. Biochem Soc Trans,2011,39(4):1028-32.
[7] Hirt H,Gorr S U. Antimicrobial peptide GL13K is effective in reducing biofilms of pseudomonas aeruginosa[J]. Antimicrob Agents Chemother,2013,57(10):4903-10.
[8] Bhardwaj G. Webster T J. Reduced bacterial growth and increased osteoblast proliferation on titanium with a nanophase Ti O2surface treatment[J]. International J Nanomedicine,2017,12:363-9.
[9] Guo Y P,Yao Y B,Guo Y J,et al. Hydrothermal fabrication of mesoporous carbonated hydroxyapatite microspheres for a drug delivery system[J]. Micropor Mesopor Mat,2012,155:245-51.
[10] Wang T,Weng Z,Liu X,et,al. Controlled release and biocompatibility of polymer/titania nanotube array system on titanium implants[J]. Bioact Mater,2017,2(1):44-50.
[11] Kazemzadeh-Narbat M,Lai B F,Ding C,et al. Multilayered coating on titanium for controlled release of antimicrobial peptides for the prevention of implant-associated infections[J]. Biomaterials,2013,34(24):5969-77.
[12]高啟坤,宋婷婷,吴齐越,等.钛纳米管表面固定成骨细胞特异性多肽的实验研究[J].安徽医科大学学报,2017,52(12):1790-94.
[13] Pawar S N,Edgar K J. Alginate derivatization:a review of chemistry,properties and applications[J]. Biomaterials,2012,33(11):3279-305.