不同品种甜橙类酚类物质检测及含量差异性研究
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摘要
甜橙类是世界栽培最广泛的柑桔种类,主要分为普通甜橙、脐橙、血橙等,具有非常高的营养价值。该研究以我国25个主要甜橙类品种为试材,采用超高效液相色谱法,检测不同品种甜橙类(普通甜橙、脐橙、血橙)及其不同部位(果皮、果肉和果汁)中酚类物质的种类和含量,比较其差异性,探讨甜橙类生物活性成分与品种之间的相互关系。结果表明,甜橙类三大基本类型的果皮、果肉、果汁中,酚类物质含量丰富,不同类型间种类和含量差异不大。甜橙类3种类型的不同代表中酚类物质具有相同的变化模式,果皮、果肉、果汁的酚类物质只存在丰度上的差异。清家脐橙(日-81-1)类黄酮含量较丰富,脐血橙酚酸物质含量较丰富,具有较高的利用价值。
Sweet orange is the most widely cultivated citrus species in the world with very high nutritional values.In this study,25 main varieties of orange in China were measured. The classification and contents of phenols in the peel,pulp,and juice of different kinds of orange( ordinary sweet orange,navel orange,and blood orange) were analyzed by ultra high performance liquid chromatography( UPLC). Besides,the relationship between bioactive compounds and varieties of oranges was discussed. The results showed that the contents of phenols were abundant and showed some differences in the peel,pulp,and juice of three basic types of orange. There were little differences in the types and contents of phenols in different types of orange,and also the changing patterns of phenols were the same. Moreover,Seike navel orange( Japan-81-1) had more abundant flavonoid content and Washington Sanguine had more abundant phenolic acids and therefore had high utilization value.
Sweet orange is the most widely cultivated citrus species in the world with very high nutritional values.In this study,25 main varieties of orange in China were measured. The classification and contents of phenols in the peel,pulp,and juice of different kinds of orange( ordinary sweet orange,navel orange,and blood orange) were analyzed by ultra high performance liquid chromatography( UPLC). Besides,the relationship between bioactive compounds and varieties of oranges was discussed. The results showed that the contents of phenols were abundant and showed some differences in the peel,pulp,and juice of three basic types of orange. There were little differences in the types and contents of phenols in different types of orange,and also the changing patterns of phenols were the same. Moreover,Seike navel orange( Japan-81-1) had more abundant flavonoid content and Washington Sanguine had more abundant phenolic acids and therefore had high utilization value.
引文
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[19] MOULEHI I,BOURGOU S,OURGHEMMI I,et al. Variety and ripening impact on phenolic composition and antioxidant activity of mandarin(Citrus reticulate Blanco)and bitter orange(Citrus aurantium L.)seeds extracts[J]. Industrial Crops and Products,2011,39:74-80.
[2] CHOE S C,KIM H S,JEONG T S,et al. Naringin has an antiatherogenic effect with the inhibition of intercellular adhesion molecule-1 in hypercholesterolemic rabbits[J]. Journal of Cardiovascular Pharmacology,2001,38(6):947-955.
[3] LI Shiming,SANG Shengmin,PAN MinHsiung,et al. Anti-inflammatory property of the urinary metabolites of nobiletin in mouse[J]. Bioorganic and Medicinal Chemistry Letters,2007,17(18):5 177-5 181.
[4] WILMSEN P K,SPADA D S,SALVADOR M. Antioxidant activity of the flavonoid hesperidin in chemical and biological systems[J]. Journal of Agricultural and Food Chemistry,2005,53(12):4 757-4 761.
[5] JEON S M,BOK S H,JANG M K,et al. Antioxidative activity of naringin and lovastatin in high cholesterol-fed rabbits[J]. Life Sciences,2001,69(24):2 855-2 866.
[6] RAMFUL D,TARNUS E,ARUOMA O I,et al. Polyphenol composition,vitamin C content and antioxidant capacity of Mauritian citrus fruits pulps[J]. Food Research International,2011,44(7):2 088-2 099.
[7]汪志辉,刘世福,严巧巧,等.石棉县黄果柑生物学特性调查与差异珠系比较[J].北方园艺,2011(14):20-24.
[8] ZHOU Yuan,HE Weizhu,ZHENG Wulin,et al. Fruit sugar and orange acid were significantly related to fruit Mg of six citrus cultivars[J]. Food Chemistry,2018,259:278-285.
[9] CLAUDIO G,SERENA G,PATRIZIA R,et al. Flavanone plasma pharmacokinetics from blood orange juice in human subjects[J]. British Journal of Nutrition,2007,98(1):165-172.
[10] GATTUSO G,BARRECA D,GARGIULLI C,et al. Flavonoid composition of citrus juices[J]. Molecules,2007,12(8):1 641-1 673.
[11]冉玥,焦必宁,赵其阳,等.超高效液相色谱法同时测定柑橘中11种类黄酮物质[J].食品科学,2013,34(4):168-173.
[12]余艳峰,祁春节.中国甜橙国际竞争力实证分析[J].新疆农垦经济,2007(7):13-19.
[13]张元梅,周志钦,孙玉敬,等.高效液相色谱法同时测定柑橘果实中18种类黄酮的含量[J].中国农业科学,2012,45(17):3 558-3 565.
[14] PETERSON J J,BEECHER G R,BHAGWAT S A,et al.Flavanones in grapefruit,lemons,and limes:A compilation and review of the data from the analytical literature[J].Journal of Food Composition and Analysis,2006,19(S):S74-S80.
[15] BOLUDA-AGUILAR M,GARCIA-VIDAL L,GONZLEZCASTAEDA,et al. Mandarin peel wastes pretreatment with steam explosion for bioethanol production[J]. Bioresource Technology,2010,101(10):3 506-3 513.
[16] HIASA S,IWAMOTO S,ENDOT,et al. Isolation of cellulose nanofibrils from mandarin(Citrus unshiu)peel waste[J]. Industrial Crops and Products,2014,62:280-285.
[17] HO S C,LIN C C. Investigation of heat treating conditions for enhancing the anti-inflammatory activity of citrus fruit(Citrus reticulata)peels[J]. Journal of Agricultural and Food Chemistry,2008,56(17):7 976-7 982.
[18] HUANG Y S,HO S C. Polymethoxy flavones are responsible for the anti-inflammatory activity of citrus fruit peel[J]. Food Chemistry,2010,119(3):868-873.
[19] MOULEHI I,BOURGOU S,OURGHEMMI I,et al. Variety and ripening impact on phenolic composition and antioxidant activity of mandarin(Citrus reticulate Blanco)and bitter orange(Citrus aurantium L.)seeds extracts[J]. Industrial Crops and Products,2011,39:74-80.