血清蛋白磷酸酯酶2A水平与卵巢癌患病的相关性研究
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摘要
目的在血清学水平探讨卵巢癌患者蛋白磷酸酯酶2A(protein phosphatase,PP2A)蛋白表达水平及其与卵巢癌肿瘤标志物糖链抗原125(sugar chain antigen 125,CA125)和人附睾蛋白4(human epididymal protein 4,HE4)水平的相关性。方法分别选取正常对照组30例,卵巢囊肿15例,卵巢癌患者20例进行研究。采用酶联免疫吸附实验(ELISA)检测血清PP2A蛋白表达水平。结果与正常对照组相比,卵巢囊肿组血清PP2A水平下降,差异有统计学意义(P<0.05),同时PP2A酶活性下调,PP2A活性仅为正常对照组的80%,差异有统计学意义(P<0.05);卵巢癌组血清PP2A水平下降明显,差异有统计学意义(P<0.01),同时酶活性显著下调,PP2A酶活性仅为正常组的65%,差异有统计学意义(P<0.01)。与卵巢囊肿组相比,卵巢癌组血清PP2A水平下调,差异有统计学意义(P<0.05),酶活性水平下调,差异有统计学意义(P<0.05)。进一步做血清PP2A水平与肿瘤标志物相关性分析,结果显示无论是卵巢囊肿组还是卵巢癌组PP2A与CA125及HE4均无相关性,仅卵巢癌组中CA125和HE4呈正相关(P<0.01)。结论卵巢癌患者血清中PP2A蛋白表达水平显著下降,血清中PP2A水平与CA125及HE4均无相关性,提示血清中PP2A水平可能为卵巢癌独立的危险因素,检测患者血清PP2A水平可早期预测卵巢癌。
[Objective]To discuss the correlation between the expression level of protein phosphatase 2 A(PP2A) and the level of sugar chain antigen 125( CA125) and human epididymal protein 4(HE4) in o varian cancer patients at serological level.[Methods]30 cases of normal controls,15 cases of ovarian cyst and 20 cases of ovarian cancer were enrolled in the study. The serum PP2A protein expression was detected by enzyme linked immunosorbent assay(ELISA).[Results]Compared with normal control group,the serum level of PP2A in ovarian cyst group decreased,the difference was statistically significant(P<0.05),while the serum activity of PP2A decreased,which was only 80% of that in the normal control group,the difference was statistically significant(P <0.05). The serum level of PP2A in ovarian cancer group decreased significantly,the difference was statistically significant(P <0.01),while the serum enzyme activity decreased,which was only 65% of that in the normal group,the difference was statistically significant(P<0.01). Compared with the ovarian cyst group,the serum PP2A level of the ovarian cancer group was significantly reduced(P<0.05),the enzyme activity decreased,and the difference was statistically significant(P<0.05). The further correlation analysis found that there were no correlations between PP2A and CA125,HE4 in both ovarian cyst group and ovarian cancer group,and CA125 was positively correlated with HE4 in ovarian cancer group(P<0.01).[Conclusion]The serum PP2A protein expression level drops significantly in ovarian cancer patients,serum PP2A level is not correlated with level of CA125 and HE4,indicating serum PP2A level may be independent risk factors for ovarian cancer,and detection of serum PP2A level can predict ovarian cancer in early stage.
[Objective]To discuss the correlation between the expression level of protein phosphatase 2 A(PP2A) and the level of sugar chain antigen 125( CA125) and human epididymal protein 4(HE4) in o varian cancer patients at serological level.[Methods]30 cases of normal controls,15 cases of ovarian cyst and 20 cases of ovarian cancer were enrolled in the study. The serum PP2A protein expression was detected by enzyme linked immunosorbent assay(ELISA).[Results]Compared with normal control group,the serum level of PP2A in ovarian cyst group decreased,the difference was statistically significant(P<0.05),while the serum activity of PP2A decreased,which was only 80% of that in the normal control group,the difference was statistically significant(P <0.05). The serum level of PP2A in ovarian cancer group decreased significantly,the difference was statistically significant(P <0.01),while the serum enzyme activity decreased,which was only 65% of that in the normal group,the difference was statistically significant(P<0.01). Compared with the ovarian cyst group,the serum PP2A level of the ovarian cancer group was significantly reduced(P<0.05),the enzyme activity decreased,and the difference was statistically significant(P<0.05). The further correlation analysis found that there were no correlations between PP2A and CA125,HE4 in both ovarian cyst group and ovarian cancer group,and CA125 was positively correlated with HE4 in ovarian cancer group(P<0.01).[Conclusion]The serum PP2A protein expression level drops significantly in ovarian cancer patients,serum PP2A level is not correlated with level of CA125 and HE4,indicating serum PP2A level may be independent risk factors for ovarian cancer,and detection of serum PP2A level can predict ovarian cancer in early stage.
引文
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[11]KAUKO O,WESTERMARCK J.Non-genomic mechanisms of protein phosph atase 2A(PP2A)regulation in cancer[J/OL].Int J Biochem Cell Biol,2018,96:157-164.[2018-03-05].https://www.ncbi.nlm.nih.gov/pubmed/29355757.DOI:10.1016/j.biocel.2018.01.005.
[12]CHENG JC,AUERSPERG N,LEUNG PC.Inhibition of p53 induces invasion of serous borderline ovarian tumor cells by accentuating PI3K/Akt-mediated suppression of E-cadherin[J].Oncogene,2011,30(9):1020-1031.
[13]LIU L,HUANG Z,CHEN J,et al.Protein phosphatase 2A mediates JS-K-induced apoptosis by affecting Bcl-2 family proteins in human hepatocellular carcinoma HepG2 cells[J].J Cell Biochem,2018,119(8):6633-6643.
[14]郑红云,李艳,申复进,等.宫颈癌患者宫颈组织中PP2A水平的研究[J].临床和实验医学杂志,2016,15(20):1973-1975.
[2]张肖肖,王玲,吴晓彤,等.卵巢癌分子靶向治疗进展[J].中国妇幼保健,2018,33(8):1918-1921.
[3]杨卫.肿瘤标志物联合检测在卵巢癌诊断中的应用[J].数理医药学杂志,2018,31(4):525-526.
[4]LIU XP,ZHENG HY,QU M,et al.Upregulation of astrocytes protein phosphatase-2A stimulates astro cytes migration via inhibiting p38MAPK in tg2576 mice[J].Glia,2012,60(9):1279-1288.
[5]郑红云,李艳,申复进.血浆蛋白磷酸酯酶2A水平与宫颈癌的相关性研究[J].检验医学与临床,2015,12(24):721-723.
[6]杨帆,谢树红.卵巢癌在代谢组学中的研究进展[J].东南国防医药,2018,20(2):160-163.
[7]郑红云,余奇,申复进,等.血清HE4、CA125和CA199联合检测对卵巢癌早期诊断的临床研究[J].国际检验医学杂志,2017,38(19):2680-2682.
[8]孙洪林,孙树凯.卵巢癌患者血清中间皮素、人附睾上皮分泌蛋白4含量与细胞浸润性生长的相关性研究[J].海南医学院学报,2017,23(18):2577-2580.
[9]郑红云,李艳.下调蛋白磷酸酯酶2A活性可抑制海马神经元轴突生长[J].卒中与神经疾病,2015,22(1):3-5.
[10]ZHU LQ,ZHENG HY,PENG CX,et al.Protein phosphatase 2A facilitates functional axonogenesis by dephosphorylating CRMP2[J].J Neurosci,2010,30(10):3839-3848.
[11]KAUKO O,WESTERMARCK J.Non-genomic mechanisms of protein phosph atase 2A(PP2A)regulation in cancer[J/OL].Int J Biochem Cell Biol,2018,96:157-164.[2018-03-05].https://www.ncbi.nlm.nih.gov/pubmed/29355757.DOI:10.1016/j.biocel.2018.01.005.
[12]CHENG JC,AUERSPERG N,LEUNG PC.Inhibition of p53 induces invasion of serous borderline ovarian tumor cells by accentuating PI3K/Akt-mediated suppression of E-cadherin[J].Oncogene,2011,30(9):1020-1031.
[13]LIU L,HUANG Z,CHEN J,et al.Protein phosphatase 2A mediates JS-K-induced apoptosis by affecting Bcl-2 family proteins in human hepatocellular carcinoma HepG2 cells[J].J Cell Biochem,2018,119(8):6633-6643.
[14]郑红云,李艳,申复进,等.宫颈癌患者宫颈组织中PP2A水平的研究[J].临床和实验医学杂志,2016,15(20):1973-1975.