紫皮石斛西洋参颗粒的研制及增强免疫力功能评价
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摘要
以紫皮石斛、西洋参为主要原料,研制具有增强免疫力功能的颗粒剂。通过正交试验,筛选最佳提取工艺;试制紫皮石斛西洋参颗粒,并进行增强免疫力功能评价。正交试验结果表明紫皮石斛西洋参颗粒的最佳提取工艺为:西洋参先用30倍量70%乙醇回流提取3 h,再与紫皮石斛一起加30倍量水,提取2次,每次4 h。增强免疫力功能实验结果显示其具有增强小鼠免疫力的功能。紫皮石斛西洋参颗粒具有良好的开发保健食品的潜力。
Granule with enhancing immunity were developed with the use of Dendrobium devonianum and Panax quinquefolium as main raw materials.The optimal extraction process of the Dendrobium devonianum Panax quinquefolium Granule was determined by orthogonal experiment.The results showed that the best extraction process of the Dendrobium devonianum Panax quinquefolium Granule was that:the Panax quinquefolium were extracted with 30 times amount of 70 % ethanol for 3 hours,and then reflux extraction with 30 times amount of water together with Dendrobium devonianum for 2 times,each time for 4 hours.Moreover,the effect of Dendrobium devonianum Panax quinquefolium Granule on immunity of mice was studied.Result showed that Dendrobium devonianum Panax quinquefolium Granule had function of enhancing immunity of mice.
Granule with enhancing immunity were developed with the use of Dendrobium devonianum and Panax quinquefolium as main raw materials.The optimal extraction process of the Dendrobium devonianum Panax quinquefolium Granule was determined by orthogonal experiment.The results showed that the best extraction process of the Dendrobium devonianum Panax quinquefolium Granule was that:the Panax quinquefolium were extracted with 30 times amount of 70 % ethanol for 3 hours,and then reflux extraction with 30 times amount of water together with Dendrobium devonianum for 2 times,each time for 4 hours.Moreover,the effect of Dendrobium devonianum Panax quinquefolium Granule on immunity of mice was studied.Result showed that Dendrobium devonianum Panax quinquefolium Granule had function of enhancing immunity of mice.
引文
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[12]李囡囡,卢莉蓉,刘芳,等.基于MATLAB优化工具箱优化西洋参总皂苷提取工艺[J].中成药,2017,39(10):2187-2190
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[15]李荣乔,贾东升,温春秀,等.槐花多糖对免疫抑制小鼠免疫功能的影响研究[J].食品研究与开发,2016,37(24):155-159
[2]陈玲玲,杨发建,王洪云,等.齿瓣石斛最新研究进展[J].亚太传统医药,2015,11(14):31-33
[3]SUN J,ZHANG F,YANG M,et al.Isolation ofα-glucosidase inhibitors including a new flavonol glycoside from Dendrobiumdevonianum[J].Nat Prod Res,2014,28(21):1900-1905
[4]DENG Y,LI M,CHEN L X,et al.Chemical characterization and immunomodulatory activity of acetylated polysaccharides from Dendrobium devonianum[J].Carbohydr Polym,2018,180:238-245
[5]中华人民共和国药典委员会.中国药典2015年版[M].北京:中国医药科技出版,2015:131-132,282-283
[6]QI B,WANG S,WANG Q,et al.Characterization and immunostimulating effects on murine peritoneal macrophages of a novel protein isolated from Panax quinquefolius L[J].J Ethnopharmacol,2016,193:700-705
[7]王洪云,王文静,马光宇,等.正交试验提取齿瓣石斛多糖工艺优化研究[J].云南中医中药杂志,2017,38(10):75-76
[8]陈盛余,赵丹丹,谢瑜,等.铁皮石斛多糖的微波辅助提取工艺研究[J].食品研究与开发,2017,38(6):49-52
[9]HU Y,ZHANG J,ZOU L,et al.Chemical characterization,antioxidant,immune-regulating and anticancer activities of a novel bioactive polysaccharide from Chenopodium quinoa seeds[J].Int J Biol Macromol,2017,99:622-629
[10]ZHANG H F,NIU L L,YANG X H,et al.Analysis of water-soluble polysaccharides in an edible medicinal plant Epimedium:method development,validation,and application[J].J AOAC Int,2014,97(3):784-790
[11]李美晨,孙敬蒙,张静,等.正交试验优选西洋参总皂苷的提取工艺[J].人参研究,2017(1):5-7
[12]李囡囡,卢莉蓉,刘芳,等.基于MATLAB优化工具箱优化西洋参总皂苷提取工艺[J].中成药,2017,39(10):2187-2190
[13]中华人民共和国卫生部.保健食品检验与评价技术规范(2003年版)[M].北京:中华人民共和国卫生部,2003:1-34,306-307
[14]张岫秀,蔡盈,吴中梅,等.连翘叶多糖对小鼠免疫功能影响的研究[J].食品研究与开发,2015,36(23):25-27
[15]李荣乔,贾东升,温春秀,等.槐花多糖对免疫抑制小鼠免疫功能的影响研究[J].食品研究与开发,2016,37(24):155-159