阴道电阻值测定法与阴道角化细胞涂片法检测蓝狐发情的比较研究
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摘要
为了比较阴道电阻值测定法和阴道角化细胞涂片法对蓝狐发情鉴定的准确率和实用性,试验选取60只青年雌种狐,随机分为2组,每组30只。Ⅰ组使用阴道电阻值测定法,Ⅱ组使用阴道角化细胞涂片法对繁殖期雌蓝狐进行发情鉴定。结果表明:Ⅰ组鉴定准确率为86. 7%,Ⅱ组鉴定准确率为56. 7%,阴道电阻值测定法鉴定蓝狐发情的准确率极显著高于阴道角化细胞涂片法(P<0. 01);阴道角化细胞涂片在蓝狐排卵±3 d内变化不明显。说明使用阴道电阻值测定法对蓝狐进行发情鉴定,其准确率优于阴道角化细胞涂片法,且快速、简便。
In order to compare the accuracy and practicability of the vaginal resistance method and vaginal keratinocyte method in the evaluation of blue foxes estrus,60 young female foxes were selected and randomly divided into two groups,30 in each group. The estrus of female blue foxes in reproductive period was identified by vaginal resistance method in group I and vaginal keratinocyte method in group Ⅱ. The results showed that the accuracy rate of identification was 86. 7% in group Ⅰ and 56. 7% in group Ⅱ,which was significantly higher( P< 0. 01) in group Ⅰ than that of in group Ⅱ. Also,the keratinocytes did not change significantly within±3 days after the blue fox's ovulation. The results suggest that the accuracy of vaginal resistance method is better than vaginal keratinocytes method for the identification of blue fox's estrus with the characteristics of rapid and simple.
In order to compare the accuracy and practicability of the vaginal resistance method and vaginal keratinocyte method in the evaluation of blue foxes estrus,60 young female foxes were selected and randomly divided into two groups,30 in each group. The estrus of female blue foxes in reproductive period was identified by vaginal resistance method in group I and vaginal keratinocyte method in group Ⅱ. The results showed that the accuracy rate of identification was 86. 7% in group Ⅰ and 56. 7% in group Ⅱ,which was significantly higher( P< 0. 01) in group Ⅰ than that of in group Ⅱ. Also,the keratinocytes did not change significantly within±3 days after the blue fox's ovulation. The results suggest that the accuracy of vaginal resistance method is better than vaginal keratinocytes method for the identification of blue fox's estrus with the characteristics of rapid and simple.
引文
[1]刘志平.影响人工授精母狐受孕率的因素[J].经济动物学报,2000,4(1):1-4.
[2] MOLLER O M. Temporal relationships between hormonal concentrations and the electrical resistance of the vaginal tract of blue foxes(Alopex lagopus)at pro-oestrus and oestrus[J]. J Reprod Fertil,1984,70(1):15-24.
[3]布隆斯泰特,芬尼,哈里霍尔.养殖狐指南[M].刘志平,译.哈尔滨:东北林业大学出版社,2010.
[4] YAMAUCHI S,NAKAMURA S,YOSHIMOTO T,et al. Prediction of the estrous cycle and optimal insemination time by monitoring vaginal electrical resistance(VER)in order to improve the reproductive efficiency of the Okinawan native Agu pig[J]. Anim Reprod Sci,2009,113(1/2/3/4):311-316.
[5]邓启发.乌苏里貉发情周期中阴道涂片及阴道电阻值变化的研究[J].河南林业科技,2005,25(1):11-13.
[6] CANFIELD R W,BUTLER W R. Accuracy of predicting the LH surge and optimal insemination time in Holstein heifers using a vaginal resistance probe[J]. Theriogenology,1989,31(4):835-842.
[7] HOCKEY C D,NORMAN S T,MORTON J M,et al. Use of vaginal electrical resistance to diagnose oestrus,dioestrus and early pregnancy in synchronized tropically adapted beef heifers[J].Reprod Domest Anim,2010,45(4):629-636.
[8] ZULUAGA J F,SALDARRIAGA J P,COOPER D A,et al. Evaluation of vaginal electrical resistance as an indicator of follicular maturity and suitability for timed artificial insemination in beef cows subjected to a synchronization of ovulation protocol[J]. Anim Reprod Sci,2008,109(1/2/3/4):17-26.
[9] GHONEIM I M,WAHEED M M,ADAM M I,et al. Relationship between the size of the dominant follicle,vaginal electrical resistance,serum concentrations of oestradiol and progesterone and sexual receptivity during the follicular phase of the dromedary camel(Camelus dromedarius)[J]. Anim Reprod Sci,2015,154:63-67.
[10] PETERS A R. Changes in electrical resistance of the vaginal mucosa in prostaglandin-treated cows[J]. Vet Rec,1989,124(19):505-507.
[11] WEI S,ZOU W,ZHANG T. Effects of GnRH agonist immunization on vaginal electrical resistance, FSH, LH, and ovaries in prepubertal female sheep[J]. J Anim Sci,2013,58(9):420-428.
[12] GUPTA K A,PUROHIT G N. Use of vaginal electrical resistance(VER)to predict estrus and ovarian activity,its relationship with plasma progesterone and its use for insemination in buffaloes[J].Theriogenology,2001,56(2):235-245.
[13] MCCAUGHEY W J. Pregnancy diagnosis in cattle by measuring vaginal electrical resistance[J]. Vet Res Commun,1981,5(1):85-90.
[2] MOLLER O M. Temporal relationships between hormonal concentrations and the electrical resistance of the vaginal tract of blue foxes(Alopex lagopus)at pro-oestrus and oestrus[J]. J Reprod Fertil,1984,70(1):15-24.
[3]布隆斯泰特,芬尼,哈里霍尔.养殖狐指南[M].刘志平,译.哈尔滨:东北林业大学出版社,2010.
[4] YAMAUCHI S,NAKAMURA S,YOSHIMOTO T,et al. Prediction of the estrous cycle and optimal insemination time by monitoring vaginal electrical resistance(VER)in order to improve the reproductive efficiency of the Okinawan native Agu pig[J]. Anim Reprod Sci,2009,113(1/2/3/4):311-316.
[5]邓启发.乌苏里貉发情周期中阴道涂片及阴道电阻值变化的研究[J].河南林业科技,2005,25(1):11-13.
[6] CANFIELD R W,BUTLER W R. Accuracy of predicting the LH surge and optimal insemination time in Holstein heifers using a vaginal resistance probe[J]. Theriogenology,1989,31(4):835-842.
[7] HOCKEY C D,NORMAN S T,MORTON J M,et al. Use of vaginal electrical resistance to diagnose oestrus,dioestrus and early pregnancy in synchronized tropically adapted beef heifers[J].Reprod Domest Anim,2010,45(4):629-636.
[8] ZULUAGA J F,SALDARRIAGA J P,COOPER D A,et al. Evaluation of vaginal electrical resistance as an indicator of follicular maturity and suitability for timed artificial insemination in beef cows subjected to a synchronization of ovulation protocol[J]. Anim Reprod Sci,2008,109(1/2/3/4):17-26.
[9] GHONEIM I M,WAHEED M M,ADAM M I,et al. Relationship between the size of the dominant follicle,vaginal electrical resistance,serum concentrations of oestradiol and progesterone and sexual receptivity during the follicular phase of the dromedary camel(Camelus dromedarius)[J]. Anim Reprod Sci,2015,154:63-67.
[10] PETERS A R. Changes in electrical resistance of the vaginal mucosa in prostaglandin-treated cows[J]. Vet Rec,1989,124(19):505-507.
[11] WEI S,ZOU W,ZHANG T. Effects of GnRH agonist immunization on vaginal electrical resistance, FSH, LH, and ovaries in prepubertal female sheep[J]. J Anim Sci,2013,58(9):420-428.
[12] GUPTA K A,PUROHIT G N. Use of vaginal electrical resistance(VER)to predict estrus and ovarian activity,its relationship with plasma progesterone and its use for insemination in buffaloes[J].Theriogenology,2001,56(2):235-245.
[13] MCCAUGHEY W J. Pregnancy diagnosis in cattle by measuring vaginal electrical resistance[J]. Vet Res Commun,1981,5(1):85-90.