子宫肌瘤患者孕激素受体的表达及意义
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摘要
目的探讨子宫肌瘤患者孕激素受体(PR)的表达及意义。方法选取2017年1月至2017年12月我院保存的子宫肌瘤组织标本21例,同时选取正常子宫平滑肌组织作为对照,采用免疫组化染色检测PR表达;原代培养子宫肌瘤细胞和正常子宫平滑肌细胞,采用Western-blot检测PR-A、PR-B和PR-M表达,同时筛选出仅表达PR-M的细胞株(PR-M阳性组)和仅表达PR-A/B的细胞株(PR-M阴性组)进行CCK-8实验。结果子宫肌瘤组织PR阳性表达率为95.24%,明显高于正常子宫平滑肌组织(P<0.05);子宫肌瘤细胞PR-A、PR-B、PR-M蛋白相对表达量分别为(0.762±0.116)、(0.987±0.107)和(0.097±0.042),明显高于子宫平滑肌细胞(P<0.05);PR-M阳性组培养24h、48h吸光值(OD)分别为(0.352±0.097)和(0.411±0.091),明显高于PR-M阴性组(P<0.05)。结论子宫肌瘤组织以及细胞PR表达升高,其中PR-M蛋白可能与子宫肌瘤细胞增殖有关,值得进一步研究。
Objective To investigate the expression and significance of progesterone receptor(PR) in patients with uterine fibroids. Methods A total of 21 specimens of hysteromyoma preserved in our hospital from January 2017 to December 2017 were selected and normal uterine smooth muscle tissue was selected as the control,the expression of PR was detected with immunohistochemical staining;The myoma cells and normal uterine smooth muscle cells were primary cultured,the expression of PR-A,PR-B and PR-M were detected by using Western-blot,the cell line(PR-M positive group) only expressing PR-M and the cell line only expressing PR-A/B(PR-M negative group) were screened out for CCK-8 test. Results The positive expression rate of PR in hysteromyoma was 95.24%,which was significantly higher than that of normal uterine smooth muscle tissue,and the difference was statistically significant(P<0.05);The relative expressions of PR-A,PR-B and PR-M in hysteromyoma cells were(0.762±0.116),(0.987±0.107) and(0.097±0.042),which were significantly higher than those in uterine smooth muscle cells(P<0.05);The absorbance values(OD) at 24 h and 48 h in PR-M positive group were(0.352±0.097) and(0.411± 0.091),which were significantly higher than those in PR-M negative group,and the difference was statistically significant(P<0.05). Conclusion The expression of PR in hysteromyoma tissues and cells are increased,and PR-M protein may be related to the proliferation of myoma cells,is worth further study.
Objective To investigate the expression and significance of progesterone receptor(PR) in patients with uterine fibroids. Methods A total of 21 specimens of hysteromyoma preserved in our hospital from January 2017 to December 2017 were selected and normal uterine smooth muscle tissue was selected as the control,the expression of PR was detected with immunohistochemical staining;The myoma cells and normal uterine smooth muscle cells were primary cultured,the expression of PR-A,PR-B and PR-M were detected by using Western-blot,the cell line(PR-M positive group) only expressing PR-M and the cell line only expressing PR-A/B(PR-M negative group) were screened out for CCK-8 test. Results The positive expression rate of PR in hysteromyoma was 95.24%,which was significantly higher than that of normal uterine smooth muscle tissue,and the difference was statistically significant(P<0.05);The relative expressions of PR-A,PR-B and PR-M in hysteromyoma cells were(0.762±0.116),(0.987±0.107) and(0.097±0.042),which were significantly higher than those in uterine smooth muscle cells(P<0.05);The absorbance values(OD) at 24 h and 48 h in PR-M positive group were(0.352±0.097) and(0.411± 0.091),which were significantly higher than those in PR-M negative group,and the difference was statistically significant(P<0.05). Conclusion The expression of PR in hysteromyoma tissues and cells are increased,and PR-M protein may be related to the proliferation of myoma cells,is worth further study.
引文
[1]Pessarrodona A,Isern J,Rodríguez J,et al.Tratamiento de los miomas uterinos mediante ultrasonidos de alta intensidad[J].Medicina Clínica,2013,141(6):22-29.
[2]Doherty L,Mutlu L,Sinclair D,et al.Uterine Fibroids[J].Reproductive Sciences,2014,21(9):1067-1092.
[3]封全灵,王智霆,刘慧云.子宫肌瘤细胞中孕激素受体M、A/B及porin蛋白表达观察[J].山东医药,2017,57(15):45-47.
[4]封全灵,熊祯祯,王智霆,等.米非司酮对孕激素受体M阳性子宫肌瘤细胞增殖、凋亡的影响[J].山东医药,2017,57(3):13-15.
[5]刘晓敏,王英红.雌、孕激素受体在子宫肌瘤中的研究进展[J].中国妇幼保健,2014,29(14):2292-2294.
[6]莫玉俏,陈小菊.腹腔镜下不同子宫肌瘤剔除方式妊娠结局的比较[J].现代仪器与医疗,2017,23(3):43-44.
[7]Wo?niak S,Szkodziak P,Czuczwar P,et al.The effect of ulipristal acetate treatment on symptomatic uterine fibroids within 12-months follow-up[J].Menopausal Review,2014,1(5):18-21.
[8]李斯静,李晓菲,张娟,等.腹腔镜超声辅助子宫肌瘤剔除术的临床应用[J].中华医学杂志,2016,96(33):2652-2654.81
[9]刘冉,陶国伟,甄军晖,等.ER、PR在妊娠期子宫肌瘤组织中的表达及意义[J].山东大学学报:医学版,2016,54(5):45-49.
[10]唐佳松,吕小娟,闫淑芬,等.PR、Fas的基因多态性与子宫肌瘤发病的相关性研究[J].吉林医学,2017,38(4):633-635.
[11]欧阳俊,黄利红.子宫肌瘤患者外周血胰岛素样生长因子水平与激素受体表达的相关性[J].中国妇幼保健,2015,30(30):5155-5157.
[12]史凡黎,宋燕,王敏,等.miR-23及孕激素受体蛋白在子宫肌瘤组织中表达及临床意义研究[J].中国实用妇科与产科杂志,2017,33(6):655-658.
[13]汪伟,李冬梅,张立会,等.雌激素受体和孕激素受体在复发子宫肌瘤组织中的表达及意义[J].中国妇幼保健,2014,29(13):2092-2094.
[14]吴海莉,宋燕,周莹莹,等.子宫肌瘤组织SULT1E1和ERα、ERβ、PRA、PRB受体蛋白的表达及临床意义[J].现代肿瘤医学,2015,23(10):1419-1424.
[15]谢建军,李健和,易利丹,等.子宫肌瘤的发病机制与药物治疗[J].中国医药导报,2011,8(1):16-18.
[16]李秋粉.血清孕酮、β-HCG、CA125以及子宫内膜厚度在诊断早期异位妊娠患者中的应用价值[J].实验与检验医学,2017,35(3):403-405.
[2]Doherty L,Mutlu L,Sinclair D,et al.Uterine Fibroids[J].Reproductive Sciences,2014,21(9):1067-1092.
[3]封全灵,王智霆,刘慧云.子宫肌瘤细胞中孕激素受体M、A/B及porin蛋白表达观察[J].山东医药,2017,57(15):45-47.
[4]封全灵,熊祯祯,王智霆,等.米非司酮对孕激素受体M阳性子宫肌瘤细胞增殖、凋亡的影响[J].山东医药,2017,57(3):13-15.
[5]刘晓敏,王英红.雌、孕激素受体在子宫肌瘤中的研究进展[J].中国妇幼保健,2014,29(14):2292-2294.
[6]莫玉俏,陈小菊.腹腔镜下不同子宫肌瘤剔除方式妊娠结局的比较[J].现代仪器与医疗,2017,23(3):43-44.
[7]Wo?niak S,Szkodziak P,Czuczwar P,et al.The effect of ulipristal acetate treatment on symptomatic uterine fibroids within 12-months follow-up[J].Menopausal Review,2014,1(5):18-21.
[8]李斯静,李晓菲,张娟,等.腹腔镜超声辅助子宫肌瘤剔除术的临床应用[J].中华医学杂志,2016,96(33):2652-2654.81
[9]刘冉,陶国伟,甄军晖,等.ER、PR在妊娠期子宫肌瘤组织中的表达及意义[J].山东大学学报:医学版,2016,54(5):45-49.
[10]唐佳松,吕小娟,闫淑芬,等.PR、Fas的基因多态性与子宫肌瘤发病的相关性研究[J].吉林医学,2017,38(4):633-635.
[11]欧阳俊,黄利红.子宫肌瘤患者外周血胰岛素样生长因子水平与激素受体表达的相关性[J].中国妇幼保健,2015,30(30):5155-5157.
[12]史凡黎,宋燕,王敏,等.miR-23及孕激素受体蛋白在子宫肌瘤组织中表达及临床意义研究[J].中国实用妇科与产科杂志,2017,33(6):655-658.
[13]汪伟,李冬梅,张立会,等.雌激素受体和孕激素受体在复发子宫肌瘤组织中的表达及意义[J].中国妇幼保健,2014,29(13):2092-2094.
[14]吴海莉,宋燕,周莹莹,等.子宫肌瘤组织SULT1E1和ERα、ERβ、PRA、PRB受体蛋白的表达及临床意义[J].现代肿瘤医学,2015,23(10):1419-1424.
[15]谢建军,李健和,易利丹,等.子宫肌瘤的发病机制与药物治疗[J].中国医药导报,2011,8(1):16-18.
[16]李秋粉.血清孕酮、β-HCG、CA125以及子宫内膜厚度在诊断早期异位妊娠患者中的应用价值[J].实验与检验医学,2017,35(3):403-405.