黄芩抑菌物的提取工艺及抑菌稳定性
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摘要
以黄芩提取物对葡萄座腔菌抑菌圈直径为衡量指标,筛选黄芩的提取工艺条件,对活性物质抑菌稳定性进行研究。结果表明,以80%乙醇作为提取溶剂时,黄芩提取物具有较强抑菌效果,在料液比1∶27.5、超声提取2.5 h、提取温度72.5℃、超声功率850 W条件下,抑菌圈直径达到31.8 mm,相比以丙酮作为提取溶剂,料液比1∶20、在40℃、700 W超声波提取2.0 h条件下的抑菌能力提高了28.69%。紫外光照射10 h以上、偏碱性环境、Ag~+、Fe~(3+)、Cu~(2+)金属离子对提取物活性均产生抑制作用,其中pH值为12时的抑菌圈直径比pH值为5时降低了44.10%;低于25℃储存40 d左右,对提取物活性的影响较小。
Based on antimicrobial circle diameter of Scutellaria baicalensis extract against Botryosphaeria dothidea,the extraction conditions of antibacterial active substances from Scutellaria baicalensis and the antibacterial stability of the active substances were determined.Experimental results show that when 80% ethanol is used as extraction solvent,the extract has strong bacteriostatic effect;the optimum extraction process condition includes ratio of material to liquid 1∶27.5,ultrasonic extraction time 2.5 h,extraction temperature 72.5 ℃,and ultrasonic power 850 W,under which the diameter of bacteriostasis circle reached 31.8 mm.The antimicrobial activity of 80% ethanol extract increased by 28.69%,compared to acetone extract in condition with ratio of material to liquid 1∶20,temperature 40 ℃,and 700 W ultrasound for 2.0 h.UV irradiation over 10 h,an alkaline environment,and the presence of metal ions Ag~+,Fe~(3+),Cu~(2+) all showed inhibition effects on the activity of Scutellaria baicalensis extract.Compared to a pH 5 environment,the diameter of bacteriostasis circle decreased by 44.10% at pH 12.The activity of extracts was less affected by storage below 25 ℃ for about 40 days.
Based on antimicrobial circle diameter of Scutellaria baicalensis extract against Botryosphaeria dothidea,the extraction conditions of antibacterial active substances from Scutellaria baicalensis and the antibacterial stability of the active substances were determined.Experimental results show that when 80% ethanol is used as extraction solvent,the extract has strong bacteriostatic effect;the optimum extraction process condition includes ratio of material to liquid 1∶27.5,ultrasonic extraction time 2.5 h,extraction temperature 72.5 ℃,and ultrasonic power 850 W,under which the diameter of bacteriostasis circle reached 31.8 mm.The antimicrobial activity of 80% ethanol extract increased by 28.69%,compared to acetone extract in condition with ratio of material to liquid 1∶20,temperature 40 ℃,and 700 W ultrasound for 2.0 h.UV irradiation over 10 h,an alkaline environment,and the presence of metal ions Ag~+,Fe~(3+),Cu~(2+) all showed inhibition effects on the activity of Scutellaria baicalensis extract.Compared to a pH 5 environment,the diameter of bacteriostasis circle decreased by 44.10% at pH 12.The activity of extracts was less affected by storage below 25 ℃ for about 40 days.
引文
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[3]ZHANG J,SI G,ZOU J,et al.Antimicrobial effects of silver nanoparticles synthesized by fatsia japonica leaf extracts for preservation of citrus fruits[J].Journal of Food Science,2017,82(8):1861-1866.
[4]ALOUI H,KHWALDIA K,SáNCHEZ-GONZáLEZ L,et al.Alginate coatings containing grapefruit essential oil or grapefruit seed extract for grapes preservation[J].International Journal of Food Science&Technology,2014,49(4):952-959.
[5]CHEN C,PENG X,ZENG R,et al.Ficus hirta fruits extract incorporated into an alginate-based edible coating for Nanfeng mandarin preservation[J].Scientia Horticulturae,2016,202:41-48.
[6]MANSO T,NUNES C.Metschnikowia andauensis,as a new biocontrol agent of fruit postharvest diseases[J].Postharvest Biology&Technology,2011,61(1):64-71.
[7]WU P,YIN Y,ZHU B,et al.Study on fresh-keeping activity of Fructus rhodomyrti extracts on fruits and vegetables[J].Acs National Meeting Book of Abstracts,2014,274:199-212.
[8]孙思胜,张化阁,李光辉,等.不同中药提取物对“夏黑”葡萄保鲜效果的影响[J].食品工业,2018(4):96-99.
[9]刘英学,刘中刚,苏兰,等.黄芩化学成分研究[J].中国药物化学杂志,2009,19(1):59-62.
[10]吉晓丼.黄芩的化学成分与药理作用研究进展[J].中医临床研究,2017,9(9):128-129.
[11]李明,杜弢,王艳.四种药用植物对植物病原真菌的抑制作用[J].中国生物防沺学报,2008(增刊1):81-84.
[12]FU Y,LEI P,HAN Y M,et al.Investigation on the process of sapindus saponin purified with macroporous adsorption resin and screening of its bacteriostasis[J].Journal of Chinese Medicinal Materials,2010,33(2):267-270.
[13]JUE L,JUN H E,YONG H W,et al.Evaluation of freshkeeping activity of chinese herbal medicine extracts and optimal concentrations test on postharvest tomatoes fruits[J].Acta Botanica Boreali-Occidentalia Sinica,2013,33(8):1682-1690.
[14]YU B B,LYU L,YU Z Y,et al.Optimization of Scutellaria baicalensis extraction process by orthogonal experiment combined with pharmacodynamic index[J].China Journal of Chinese Materia Medica,2013,38(24):4314-4317.
[15]葛慧芳,陈继承,张孝林,等.黄芩抗金黄葡萄球菌提取工艺的优化[J].热带生物学报,2017,8(2):220-226.
[16]YI Y,WANG Y,LIU H.Preparation of new crosslinked chitosan with crown ether and their adsorption for silver ion for antibacterial activities[J].Carbohydrate Polymers,2003,53(4):425-430.
[17]BRIAN P W.Inhibition of catechol bacteriostasis by molybdates[J].Nature,1947,159:373-378.
[18]PENG W X,ZHANG Z F,ZHI L,et al.Molecular characteristics of biomedical and bacteriostasis extractives of Illicium verum fruit[J].Journal of Pure&Applied Microbiology,2013,7(3):2017-2024.