昆虫学案例在遗传学教学中的应用
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摘要
遗传学是相关专业本科生的核心课程之一。国内大部分高校在遗传学教学中使用普通遗传学教材,但不同专业背景的学生使用普通遗传学教材会产生较大的教学质量差异,特别是蚕学、蜂学、植物保护、动物科学等专业的本科生。虽然普通遗传学教材中有一些昆虫学案例,但没有随着昆虫学发展进行更新,且不能满足昆虫相关专业学生的专业素质培养需求。因此,本文对可应用于遗传学课程的昆虫学案例进行梳理,引入遗传学教学,更替部分普通遗传学教材中的非经典案例,并补充一些普通遗传学教材中使用较少的昆虫发育遗传学、核外遗传学和进化遗传学等案例。建立适用于昆虫学相关专业的遗传学理论体系与实验教学设计,使遗传学教学突出昆虫学相关专业的特色,以期对相关专业的学生学习和教师教学有所帮助。
Genetics is one of the core courses for undergraduates majoring in related disciplines. Most universities use general genetics textbooks in teaching, which will lead to different teaching qualities for students in different majors,especially in sericulture, apiculture, plant protection, and animal science. This is especially true for students majoring in insects. Although there are some entomology cases in general textbooks, they have not been updated with developments in entomology and cannot meet the needs of comprehensive quality training. Therefore, we review entomology cases that can be used in genetics courses, introduce genetics teaching points, offers replacements for some non-classical cases in general genetics textbooks, and supplement several cases of insect developmental genetics, extranuclear genetics, and evolutionary genetics, which are seldom described in general genetics textbooks. We also establish a genetics theoretical system and experimental teaching design for entomology-related majors to highlight the characteristics of entomologyrelated majors in genetics teaching, aiming to help students and teachers in related majors.
Genetics is one of the core courses for undergraduates majoring in related disciplines. Most universities use general genetics textbooks in teaching, which will lead to different teaching qualities for students in different majors,especially in sericulture, apiculture, plant protection, and animal science. This is especially true for students majoring in insects. Although there are some entomology cases in general textbooks, they have not been updated with developments in entomology and cannot meet the needs of comprehensive quality training. Therefore, we review entomology cases that can be used in genetics courses, introduce genetics teaching points, offers replacements for some non-classical cases in general genetics textbooks, and supplement several cases of insect developmental genetics, extranuclear genetics, and evolutionary genetics, which are seldom described in general genetics textbooks. We also establish a genetics theoretical system and experimental teaching design for entomology-related majors to highlight the characteristics of entomologyrelated majors in genetics teaching, aiming to help students and teachers in related majors.
引文
[1]Wang QM,Zhao G.An overview on case method researches at home and abroad.J Ningbo Univ(Educ Sci Ed),2009,31(3):7-11.王青梅,赵革.国内外案例教学法研究综述.宁波大学学报(教育科学版),2009,31(3):7-11.
[2]Wang QM,Cui JG,Yu CZ,Zhang Z,Wu YL,Zhang LJ,Lin M.Application of case teaching in genetics courses to students majoring in forestry.Hereditas(Beijing),2017,39(10):939-946.王钦美,崔建国,于长志,张智,吴月亮,张丽杰,林梅.案例教学在林学专业遗传学教学中的应用.遗传,2017,39(10):939-946.
[3]He ZM,Bie LS,Li W.Application of medical cases in general genetics teaching in universities.Hereditas(Beijing),2018,40(1):75-85.贺竹梅,别林赛,李蔚.医学病例在高校普通遗传学教学中的运用.遗传,2018,40(1):75-85.
[4]Kang L.Miraculous insects.Chin Nat,2016,4:1.康乐.神奇的昆虫.大自然,2016,(4):1.
[5]Zhang QW.Insect genetics.Beijing:Science Press,2000.张青文.昆虫遗传学.北京:科学出版社,2000.
[6]Marian R.Goldsmith and Frantisek Marec.Molecular biology and genetics of the Lepidoptera.Chongqing:Southwest China Normal University Press,2011.玛丽安·戈德史密斯,弗兰蒂塞克·马莱克.鳞翅目昆虫的分子生物学和遗传学.重庆:西南师范大学出版社,2011.
[7]Peng JX,Yang H,Hong HZ.Insect molecular genetics.Wuhan:Central China Normal University Press,2006.彭建新,杨红,洪华珠.昆虫分子遗传学.武汉:华中师范大学出版社,2006.
[8]Liu ZD,Qiao SY,Wu YH,Zhao SY.Genetics.3rd ed.Beijing:Higher Education Press,2013.刘祖洞,乔守怡,吴燕华,赵寿元.遗传学(第三版).北京:高等教育出版社,2013.
[9]Xing WJ,Morigen,Damirin A,Su HM.Restructuring teaching contents of genetics by focusing on the genetic information and its connection with other courses.Hereditas(Beijing),2011,33(6):661-664.邢万金,莫日根,阿拉坦高勒,苏慧敏.以遗传信息为主线的遗传学教学架构及与其他课程的衔接.遗传,2011,33(6):661-664.
[10]Xu J,Dong Q,Yu Y,Niu B,Ji D,Li M,Huang Y,Chen X,Tan A.Mass spider silk production through targeted gene replacement in Bombyx mori.Proc Natl Acad Sci USA,2018,115(35):8757-8762.
[11]He ZM.Modern genetics analysis:from gene to phenotype.3rd ed.Beijing:Higher Education Press,2017.贺竹梅.现代遗传学教程:从基因到表型的剖析(第3版).北京:高等教育出版社,2017.
[12]Lv HS.Principles of sex control in the silkworm.Shanghai:Shanghai Scientific and Technical Publishers,2010.吕鸿声.家蚕性别控制原理.上海:上海科学技术出版社,2010.
[13]Sima YH.Study on construction of molecular linkage map and location of QTLs controlling cocoon traits in silkworm[D].Southwest Agricultural University,2003.司马杨虎.家蚕分子图谱的构建及茧质性状的QTL定位研究[学位论文].西南农业大学,2003.
[14]Shi W,Ding GL,Zhang XL,Lv LP.The developing history of honeybee genetices.Apicul China,2006,57(6):21-22.石巍,丁桂玲,张秀琳,吕丽萍.蜜蜂遗传学发展简史.中国蜂业,2006,57(6):21-22.
[15]Van Leeuwen T,Tirry L,Nauen R.Complete maternal inheritance of bifenazate resistance in Tetranychus urticae Koch(Acari:Tetranychidae)and its implications in mode of action considerations.Insect Biochem Molec,2006,36(11):869-877.
[16]Kageyama D,Ohno M,Sasaki T,Yoshido A,Konagaya T,Jouraku A,Kuwazaki S,Kanamori H,Katayose Y,Narita S,Miyata M,Riegler M,Sahara K.Feminizing Wolbachia endosymbiont disrupts maternal sex chromosome inheritance in a butterfly species.Evol Lett,2017,1(5):232-244.
[17]Xia Q,Zhou Z,Lu C,Cheng D,Dai F,Li B,Zhao P,Zha X,Cheng T,Chai C,Pan G,Xu J,Liu C,Lin Y,Qian J,Hou Y,Wu Z,Li G,Pan M,Li C,Shen Y,Lan X,Yuan L,Li T,Xu H,Yang G,Wan Y,Zhu Y,Yu M,Shen W,Wu D,Xiang Z,Yu J,Wang J,Li R,Shi J,Li H,Li G,Su J,Wang X,Li G,Zhang Z,Wu Q,Li J,Zhang Q,Wei N,Xu J,Sun H,Dong L,Liu D,Zhao S,Zhao X,Meng Q,Lan F,Huang X,Li Y,Fang L,Li C,Li D,Sun Y,Zhang Z,Yang Z,Huang Y,Xi Y,Qi Q,He D,Huang H,Zhang X,Wang Z,Li W,Cao Y,Yu Y,Yu H,Li J,Ye J,Chen H,Zhou Y,Liu B,Wang J,Ye J,Ji H,Li S,Ni P,Zhang J,Zhang Y,Zheng H,Mao B,Wang W,Ye C,Li S,Wang J,Wong GK,Yang H.A draft sequence for the genome of the domesticated silkworm(Bombyx mori).Science,2004,306(5703):1937-1940.
[18]Li Z,Zeng B,Ling L,Xu J,You L,Aslam AF,Tan A,Huang Y.Enhancement of larval RNAi efficiency by over-expressing Argonaute2 in Bombyx mori.Int J Biol Sci,2015,11(2):176-185.
[19]Wei W,Xin H,Roy B,Dai J,Miao Y,Gao G.Heritable genome editing with CRISPR/Cas9 in the silkworm,Bombyx mori.PLoS One,2014,9(7):e101210.
[20]Futahashi R,Shirataki H,Narita T,Mita K,Fujiwara H.Comprehensive microarray-based analysis for stage-specific larval camouflage pattern-associated genes in the swallowtail butterfly,Papilio xuthus.BMC Biol,2012,10:46.
[21]Tan A,Fu G,Jin L,Guo Q,Li Z,Niu B,Meng Z,Morrison NI,Alphey L,Huang Y.Transgene-based,female-specific lethality system for genetic sexing of the silkworm,Bombyx mori.Proc Natl Acad Sci USA,2013,110(17):6766-6770.
[22]Jiang L,Xia Q.The progress and future of enhancing antiviral capacity by transgenic technology in the silkworm Bombyx mori.Insect Biochem Molec,2014,48:1-7.
[23]Li ZQ,Chen K,Yang FY,Huang YP.Application of genetic regulation technique for invasive pest management.Bull Chin Acad Sci,2017,32(8):836-844.李芝倩,陈凯,杨芳颖,黄勇平.适用于入侵害虫治理的遗传调控技术.中国科学院院刊,2017,32(8):836-844.
[24]Crosland MW,Crozier RH.Myrmecia pilosula,an Ant with only one pair of chromosomes.Science,1986,231(4743):1278.
[25]Lukhtanov VA.The blue butterfly Polyommatus(Plebicula)atlanticus(Lepidoptera,Lycaenidae)holds the record of the highest number of chromosomes in the non-polyploid eukaryotic organisms.Comp Cytogenet,2015,9(4):683-690.
[26]Kan L,Grozhik AV,Vedanayagam J,Patil DP,Pang N,Lim KS,Huang YC,Joseph B,Lin CJ,Despic V,Guo J,Yan D,Kondo S,Deng WM,Dedon PC,Jaffrey SR,Lai EC.The m6A pathway facilitates sex determination in Drosophila.Nat Commun,2017,8:15737.
[27]Guo J,Tang HW,Li J,Perrimon N,Yan D.Xio is a component of the Drosophila sex determination pathway and RNA N6-methyladenosine methyltransferase complex.Proc Natl Acad Sci USA,2018,115(14):3674-3679.
[28]Kawaoka S,Kadota K,Arai Y,Suzuki Y,Fujii T,Abe H,Yasukochi Y,Mita K,Sugano S,Shimizu K,Tomari Y,Shimada.The silkworm W chromosome is a source of female-enriched piRNAs.RNA,2011,17(12):2144-2151.
[29]Kiuchi T,Koga H,Kawamoto M,Shoji K,Sakai H,Arai Y,Ishihara G,Kawaoka S,Sugano S,Shimada T,Suzuki Y,Suzuki MG,Katsuma S.A single female-specific piRNA is the primary determiner of sex in the silkworm.Nature,2014,509(7502):633-636.
[30]Li Z,You L,Yan D,James AA,Huang Y,Tan A.Bombyx mori histone methyltransferase BmAsh2 is essential for silkworm piRNA-mediated sex determination.PLoS Genet,2018,14(2):e1007245.
[31]Xu J,Chen S,Zeng B,James AA,Tan A,Huang Y.Bombyx mori P-element somatic inhibitor(BmPSI)is a key auxiliary factor for silkworm male sex determination.PLoS Genet,2017,13(1):e1006576.
[32]Mo JC,Tang ZH.Application of quantitative genetics in evolutionary study of pest population resistance.Chin Bul Entomol Knowl,1997,34(3):183-186.莫建初,唐振华.数量遗传学在害虫种群抗性进化研究中的应用.昆虫知识,1997,34(3):183-186.
[33]Luo AR,Zhang YZ,Ding L,Zhu CD.Research advances on QTL of honey bees in the post-genomic era.Acta Entomol Sin,2007,50(9):950-956.罗阿蓉,张彦周,丁亮,朱朝东.后基因组时代的蜜蜂QTL研究.昆虫学报,2007,50(9):950-956.
[34]Bakke MJ,Agusti C,Bruusgaard JC,Sundaram AYM,Horsberg TE.Deltamethrin resistance in the salmon louse,lepeophtheirus salmonis(Kr?yer):maternal inheritance and reduced apoptosis.Sci Rep,2018,8(1):8450.
[35]Knorr E,Schmidtberg H,Arslan D,Bingsohn L,Vilcinskas A.Translocation of bacteria from the gut to the eggs triggers maternal transgenerational immune priming in tribolium castaneum.Biol Lett,2015,11(12):20150885.
[36]Freitak D,Schmidtberg H,Dickel F,Lochnit G,Vogel H,Vilcinskas A.The maternal transfer of bacteria can mediate trans-generational immune priming in insects.Virulence,2014,5(4):547-554.
[37]Dong P,Wang JJ.Reproductive manipulation of Wolbachia to its hosts.Chin Bul Entomol,2006,43(3):288-294.董鹏,王进军.沃尔巴克氏体Wolbachia对宿主的生殖调控作用及其研究进展.昆虫知识,2006,43(3):288-294.
[38]Kondo N,Nikoh N,Ijichi N,Shimada M,Fukatsu T.Genome fragment of Wolbachia endosymbiont transferred to X chromosome of host insect.Proc Natl Acad Sci USA,2002,99(22):14280-14285.
[39]Hou L,Zhan S,Zhou X,Li F,Wang XH.Advances in research on insect genomics in China.Chin Bull Entomol,2017,54(5):693-704.侯丽,詹帅,周欣,李飞,王宪辉.中国昆虫基因组学的研究进展.应用昆虫学报,2017,54(5):693-704.
[40]Shi YY,Wang ZL,Zeng ZJ.Advances in research on epigenetics and caste differentiation in the honey bee.Chin Bull Entomol,2014,51(6):1406-1412.石元元,王子龙,曾志将.表观遗传学与蜜蜂级型分化的研究进展.应用昆虫学报,2014,51(6):1406-1412.
[41]Graveley BR,Brooks AN,Carlson JW,Duff MO,Landolin JM,Yang L,Artieri CG,van Baren MJ,Boley N,Booth BW,Brown JB,Cherbas L,Davis CA,Dobin A,Li R,Lin W,Malone JH,Mattiuzzo NR,Miller D,Sturgill D,Tuch BB,Zaleski C,Zhang D,Blanchette M,Dudoit S,Eads B,Green RE,Hammonds A,Jiang L,Kapranov P,Langton L,Perrimon N,Sandler JE,Wan KH,Willingham A,Zhang Y,Zou Y,Andrews J,Bickel PJ,Brenner SE,Brent MR,Cherbas P,Gingeras TR,Hoskins RA,Kaufman TC,Oliver B,Celniker SE.The developmental transcriptome of Drosophila melanogaster.Nature,2011,471(7339):473-479.
[42]Zeng B,Huang Y,Xu J,Shiotsuki T,Bai H,Palli SR,Huang Y,Tan A.The FOXO transcription factor controls insect growth and development by regulating juvenile hormone degradation in the silkworm,Bombyx mori.JBiol Chem,2017,292(28):11659-11669.
[43]Ma C,Kang L.Population genetics and the subspecific taxonomy of the migratory locust.Chin Bull Entomol,2013,50(1):1-8.马川,康乐.飞蝗的种群遗传学与亚种地位.应用昆虫学报,2013,50(1):1-8.
[44]Li HS,Pang H.Population genetics of predatory ladybirds and its applications in biological control.J Enviro Entomol,2016,38(2):221-227.李浩森,庞虹.天敌瓢虫种群遗传学及其在生物防治中的应用.环境昆虫学报,2016,38(2):221-227.
[45]Tao HM,Cai ZH,He ZB,Cao K,Wu XF.Observation of chromosome meiosis of locusta migratoria manilensis.JChongqing Norm Univ(Nat Sci Ed),2010,27(2):20-22.陶红梅,蔡志华,何正波,曹锴,吴学芳.东亚飞蝗染色体的减数分裂观察.重庆师范大学学报(自然科学版),2010,27(2):20-22.
[46]Fu JY,Zhang QW,Cai QN,Xu J,Ma XM,Zhang LS.Observations on mitotic and meiotic chromosomes of the beet armyworm,spodoptera exigua hübner(Lepidoptera:Noctuidae).Acta Entomol Sin,2005,48(1):135-138.
[47]Elwess NL,Latourelle SM,Myers L.DNA barcoding of stoneflies(Plecoptera)in a general genetics course.J Biol Educ,2018,52(4):406-414.
[48]Li RN,Wang Q,Li XH,Deng XM,Wu CX.Example of molecular genetic experimental design in teaching of Animal Genetics Experiment.Exp Technol Manag,2017,34(7):207-210.李荣妮,王勤,李新海,邓学梅,吴常信.“动物遗传学实验”教学中分子遗传实验设计举例.实验技术与管理,2017,34(7):207-210.
[49]Duffy JB.GAL4 system in Drosophila:a fly geneticist's Swiss army knife.Genesis,2002,34(1-2):1-15.
[50]Li KX,Ma EB,Zhang JZ,Zhao YD,Zhang XB.Combination of Gal80tsand Gal4 flexibly manipulates the expression levels of UAS transgenes in drosophila.Acta Entomol Sin,2016,59(5):481-488.李开霞,马恩波,张建珍,赵一丹,张徐波.Gal80ts与Gal4组合灵敏控制果蝇中UAS转基因的表达水平.昆虫学报,2016,59(5):481-488.
[51]Deng YW,Li JH.Teaching reform of genetics experiment course based on improving college students'innovative ability.Course Educ Res,2018,(7):247-248.邓岳文,李俊辉.基于提高大学生创新能力的遗传学实验课教学改革.课程教育研究,2018,(7):247-248.
[52]Pi Y,Su MJ,Wei LQ,Lu DR,Qiao SY.An exploration of teaching in the genetic experiments--developing a type with classic experiments and new technology fused(1).Biol Teach Univ Elect Edit,2017,7(3):53-57.皮妍,苏明杰,韦理强,卢大儒,乔守怡.遗传学实验的教学改革--经典实验与新技术相融合(一).高校生物学教学研究(电子版),2017,7(3):53-57.
[53]Guo B,Mo AW,Cai L,Qiao SY,Pi Y.An exploration of teaching in the genetic experiments--developing a type with classic experiments and new technology fused(2).Biol Teach Univ Elect Edit,2018,8(1):56-60.郭滨,莫翱溦,蔡亮,乔守怡,皮妍.遗传学实验的教学改革--经典实验与新技术的融合(二).高校生物学教学研究(电子版),2018,8(1):56-60.
[54]Li BC.Animal genetics.Beijing:China Agricultural University Press,2008.李碧春.动物遗传学.北京:中国农业大学出版社,2008.
[2]Wang QM,Cui JG,Yu CZ,Zhang Z,Wu YL,Zhang LJ,Lin M.Application of case teaching in genetics courses to students majoring in forestry.Hereditas(Beijing),2017,39(10):939-946.王钦美,崔建国,于长志,张智,吴月亮,张丽杰,林梅.案例教学在林学专业遗传学教学中的应用.遗传,2017,39(10):939-946.
[3]He ZM,Bie LS,Li W.Application of medical cases in general genetics teaching in universities.Hereditas(Beijing),2018,40(1):75-85.贺竹梅,别林赛,李蔚.医学病例在高校普通遗传学教学中的运用.遗传,2018,40(1):75-85.
[4]Kang L.Miraculous insects.Chin Nat,2016,4:1.康乐.神奇的昆虫.大自然,2016,(4):1.
[5]Zhang QW.Insect genetics.Beijing:Science Press,2000.张青文.昆虫遗传学.北京:科学出版社,2000.
[6]Marian R.Goldsmith and Frantisek Marec.Molecular biology and genetics of the Lepidoptera.Chongqing:Southwest China Normal University Press,2011.玛丽安·戈德史密斯,弗兰蒂塞克·马莱克.鳞翅目昆虫的分子生物学和遗传学.重庆:西南师范大学出版社,2011.
[7]Peng JX,Yang H,Hong HZ.Insect molecular genetics.Wuhan:Central China Normal University Press,2006.彭建新,杨红,洪华珠.昆虫分子遗传学.武汉:华中师范大学出版社,2006.
[8]Liu ZD,Qiao SY,Wu YH,Zhao SY.Genetics.3rd ed.Beijing:Higher Education Press,2013.刘祖洞,乔守怡,吴燕华,赵寿元.遗传学(第三版).北京:高等教育出版社,2013.
[9]Xing WJ,Morigen,Damirin A,Su HM.Restructuring teaching contents of genetics by focusing on the genetic information and its connection with other courses.Hereditas(Beijing),2011,33(6):661-664.邢万金,莫日根,阿拉坦高勒,苏慧敏.以遗传信息为主线的遗传学教学架构及与其他课程的衔接.遗传,2011,33(6):661-664.
[10]Xu J,Dong Q,Yu Y,Niu B,Ji D,Li M,Huang Y,Chen X,Tan A.Mass spider silk production through targeted gene replacement in Bombyx mori.Proc Natl Acad Sci USA,2018,115(35):8757-8762.
[11]He ZM.Modern genetics analysis:from gene to phenotype.3rd ed.Beijing:Higher Education Press,2017.贺竹梅.现代遗传学教程:从基因到表型的剖析(第3版).北京:高等教育出版社,2017.
[12]Lv HS.Principles of sex control in the silkworm.Shanghai:Shanghai Scientific and Technical Publishers,2010.吕鸿声.家蚕性别控制原理.上海:上海科学技术出版社,2010.
[13]Sima YH.Study on construction of molecular linkage map and location of QTLs controlling cocoon traits in silkworm[D].Southwest Agricultural University,2003.司马杨虎.家蚕分子图谱的构建及茧质性状的QTL定位研究[学位论文].西南农业大学,2003.
[14]Shi W,Ding GL,Zhang XL,Lv LP.The developing history of honeybee genetices.Apicul China,2006,57(6):21-22.石巍,丁桂玲,张秀琳,吕丽萍.蜜蜂遗传学发展简史.中国蜂业,2006,57(6):21-22.
[15]Van Leeuwen T,Tirry L,Nauen R.Complete maternal inheritance of bifenazate resistance in Tetranychus urticae Koch(Acari:Tetranychidae)and its implications in mode of action considerations.Insect Biochem Molec,2006,36(11):869-877.
[16]Kageyama D,Ohno M,Sasaki T,Yoshido A,Konagaya T,Jouraku A,Kuwazaki S,Kanamori H,Katayose Y,Narita S,Miyata M,Riegler M,Sahara K.Feminizing Wolbachia endosymbiont disrupts maternal sex chromosome inheritance in a butterfly species.Evol Lett,2017,1(5):232-244.
[17]Xia Q,Zhou Z,Lu C,Cheng D,Dai F,Li B,Zhao P,Zha X,Cheng T,Chai C,Pan G,Xu J,Liu C,Lin Y,Qian J,Hou Y,Wu Z,Li G,Pan M,Li C,Shen Y,Lan X,Yuan L,Li T,Xu H,Yang G,Wan Y,Zhu Y,Yu M,Shen W,Wu D,Xiang Z,Yu J,Wang J,Li R,Shi J,Li H,Li G,Su J,Wang X,Li G,Zhang Z,Wu Q,Li J,Zhang Q,Wei N,Xu J,Sun H,Dong L,Liu D,Zhao S,Zhao X,Meng Q,Lan F,Huang X,Li Y,Fang L,Li C,Li D,Sun Y,Zhang Z,Yang Z,Huang Y,Xi Y,Qi Q,He D,Huang H,Zhang X,Wang Z,Li W,Cao Y,Yu Y,Yu H,Li J,Ye J,Chen H,Zhou Y,Liu B,Wang J,Ye J,Ji H,Li S,Ni P,Zhang J,Zhang Y,Zheng H,Mao B,Wang W,Ye C,Li S,Wang J,Wong GK,Yang H.A draft sequence for the genome of the domesticated silkworm(Bombyx mori).Science,2004,306(5703):1937-1940.
[18]Li Z,Zeng B,Ling L,Xu J,You L,Aslam AF,Tan A,Huang Y.Enhancement of larval RNAi efficiency by over-expressing Argonaute2 in Bombyx mori.Int J Biol Sci,2015,11(2):176-185.
[19]Wei W,Xin H,Roy B,Dai J,Miao Y,Gao G.Heritable genome editing with CRISPR/Cas9 in the silkworm,Bombyx mori.PLoS One,2014,9(7):e101210.
[20]Futahashi R,Shirataki H,Narita T,Mita K,Fujiwara H.Comprehensive microarray-based analysis for stage-specific larval camouflage pattern-associated genes in the swallowtail butterfly,Papilio xuthus.BMC Biol,2012,10:46.
[21]Tan A,Fu G,Jin L,Guo Q,Li Z,Niu B,Meng Z,Morrison NI,Alphey L,Huang Y.Transgene-based,female-specific lethality system for genetic sexing of the silkworm,Bombyx mori.Proc Natl Acad Sci USA,2013,110(17):6766-6770.
[22]Jiang L,Xia Q.The progress and future of enhancing antiviral capacity by transgenic technology in the silkworm Bombyx mori.Insect Biochem Molec,2014,48:1-7.
[23]Li ZQ,Chen K,Yang FY,Huang YP.Application of genetic regulation technique for invasive pest management.Bull Chin Acad Sci,2017,32(8):836-844.李芝倩,陈凯,杨芳颖,黄勇平.适用于入侵害虫治理的遗传调控技术.中国科学院院刊,2017,32(8):836-844.
[24]Crosland MW,Crozier RH.Myrmecia pilosula,an Ant with only one pair of chromosomes.Science,1986,231(4743):1278.
[25]Lukhtanov VA.The blue butterfly Polyommatus(Plebicula)atlanticus(Lepidoptera,Lycaenidae)holds the record of the highest number of chromosomes in the non-polyploid eukaryotic organisms.Comp Cytogenet,2015,9(4):683-690.
[26]Kan L,Grozhik AV,Vedanayagam J,Patil DP,Pang N,Lim KS,Huang YC,Joseph B,Lin CJ,Despic V,Guo J,Yan D,Kondo S,Deng WM,Dedon PC,Jaffrey SR,Lai EC.The m6A pathway facilitates sex determination in Drosophila.Nat Commun,2017,8:15737.
[27]Guo J,Tang HW,Li J,Perrimon N,Yan D.Xio is a component of the Drosophila sex determination pathway and RNA N6-methyladenosine methyltransferase complex.Proc Natl Acad Sci USA,2018,115(14):3674-3679.
[28]Kawaoka S,Kadota K,Arai Y,Suzuki Y,Fujii T,Abe H,Yasukochi Y,Mita K,Sugano S,Shimizu K,Tomari Y,Shimada.The silkworm W chromosome is a source of female-enriched piRNAs.RNA,2011,17(12):2144-2151.
[29]Kiuchi T,Koga H,Kawamoto M,Shoji K,Sakai H,Arai Y,Ishihara G,Kawaoka S,Sugano S,Shimada T,Suzuki Y,Suzuki MG,Katsuma S.A single female-specific piRNA is the primary determiner of sex in the silkworm.Nature,2014,509(7502):633-636.
[30]Li Z,You L,Yan D,James AA,Huang Y,Tan A.Bombyx mori histone methyltransferase BmAsh2 is essential for silkworm piRNA-mediated sex determination.PLoS Genet,2018,14(2):e1007245.
[31]Xu J,Chen S,Zeng B,James AA,Tan A,Huang Y.Bombyx mori P-element somatic inhibitor(BmPSI)is a key auxiliary factor for silkworm male sex determination.PLoS Genet,2017,13(1):e1006576.
[32]Mo JC,Tang ZH.Application of quantitative genetics in evolutionary study of pest population resistance.Chin Bul Entomol Knowl,1997,34(3):183-186.莫建初,唐振华.数量遗传学在害虫种群抗性进化研究中的应用.昆虫知识,1997,34(3):183-186.
[33]Luo AR,Zhang YZ,Ding L,Zhu CD.Research advances on QTL of honey bees in the post-genomic era.Acta Entomol Sin,2007,50(9):950-956.罗阿蓉,张彦周,丁亮,朱朝东.后基因组时代的蜜蜂QTL研究.昆虫学报,2007,50(9):950-956.
[34]Bakke MJ,Agusti C,Bruusgaard JC,Sundaram AYM,Horsberg TE.Deltamethrin resistance in the salmon louse,lepeophtheirus salmonis(Kr?yer):maternal inheritance and reduced apoptosis.Sci Rep,2018,8(1):8450.
[35]Knorr E,Schmidtberg H,Arslan D,Bingsohn L,Vilcinskas A.Translocation of bacteria from the gut to the eggs triggers maternal transgenerational immune priming in tribolium castaneum.Biol Lett,2015,11(12):20150885.
[36]Freitak D,Schmidtberg H,Dickel F,Lochnit G,Vogel H,Vilcinskas A.The maternal transfer of bacteria can mediate trans-generational immune priming in insects.Virulence,2014,5(4):547-554.
[37]Dong P,Wang JJ.Reproductive manipulation of Wolbachia to its hosts.Chin Bul Entomol,2006,43(3):288-294.董鹏,王进军.沃尔巴克氏体Wolbachia对宿主的生殖调控作用及其研究进展.昆虫知识,2006,43(3):288-294.
[38]Kondo N,Nikoh N,Ijichi N,Shimada M,Fukatsu T.Genome fragment of Wolbachia endosymbiont transferred to X chromosome of host insect.Proc Natl Acad Sci USA,2002,99(22):14280-14285.
[39]Hou L,Zhan S,Zhou X,Li F,Wang XH.Advances in research on insect genomics in China.Chin Bull Entomol,2017,54(5):693-704.侯丽,詹帅,周欣,李飞,王宪辉.中国昆虫基因组学的研究进展.应用昆虫学报,2017,54(5):693-704.
[40]Shi YY,Wang ZL,Zeng ZJ.Advances in research on epigenetics and caste differentiation in the honey bee.Chin Bull Entomol,2014,51(6):1406-1412.石元元,王子龙,曾志将.表观遗传学与蜜蜂级型分化的研究进展.应用昆虫学报,2014,51(6):1406-1412.
[41]Graveley BR,Brooks AN,Carlson JW,Duff MO,Landolin JM,Yang L,Artieri CG,van Baren MJ,Boley N,Booth BW,Brown JB,Cherbas L,Davis CA,Dobin A,Li R,Lin W,Malone JH,Mattiuzzo NR,Miller D,Sturgill D,Tuch BB,Zaleski C,Zhang D,Blanchette M,Dudoit S,Eads B,Green RE,Hammonds A,Jiang L,Kapranov P,Langton L,Perrimon N,Sandler JE,Wan KH,Willingham A,Zhang Y,Zou Y,Andrews J,Bickel PJ,Brenner SE,Brent MR,Cherbas P,Gingeras TR,Hoskins RA,Kaufman TC,Oliver B,Celniker SE.The developmental transcriptome of Drosophila melanogaster.Nature,2011,471(7339):473-479.
[42]Zeng B,Huang Y,Xu J,Shiotsuki T,Bai H,Palli SR,Huang Y,Tan A.The FOXO transcription factor controls insect growth and development by regulating juvenile hormone degradation in the silkworm,Bombyx mori.JBiol Chem,2017,292(28):11659-11669.
[43]Ma C,Kang L.Population genetics and the subspecific taxonomy of the migratory locust.Chin Bull Entomol,2013,50(1):1-8.马川,康乐.飞蝗的种群遗传学与亚种地位.应用昆虫学报,2013,50(1):1-8.
[44]Li HS,Pang H.Population genetics of predatory ladybirds and its applications in biological control.J Enviro Entomol,2016,38(2):221-227.李浩森,庞虹.天敌瓢虫种群遗传学及其在生物防治中的应用.环境昆虫学报,2016,38(2):221-227.
[45]Tao HM,Cai ZH,He ZB,Cao K,Wu XF.Observation of chromosome meiosis of locusta migratoria manilensis.JChongqing Norm Univ(Nat Sci Ed),2010,27(2):20-22.陶红梅,蔡志华,何正波,曹锴,吴学芳.东亚飞蝗染色体的减数分裂观察.重庆师范大学学报(自然科学版),2010,27(2):20-22.
[46]Fu JY,Zhang QW,Cai QN,Xu J,Ma XM,Zhang LS.Observations on mitotic and meiotic chromosomes of the beet armyworm,spodoptera exigua hübner(Lepidoptera:Noctuidae).Acta Entomol Sin,2005,48(1):135-138.
[47]Elwess NL,Latourelle SM,Myers L.DNA barcoding of stoneflies(Plecoptera)in a general genetics course.J Biol Educ,2018,52(4):406-414.
[48]Li RN,Wang Q,Li XH,Deng XM,Wu CX.Example of molecular genetic experimental design in teaching of Animal Genetics Experiment.Exp Technol Manag,2017,34(7):207-210.李荣妮,王勤,李新海,邓学梅,吴常信.“动物遗传学实验”教学中分子遗传实验设计举例.实验技术与管理,2017,34(7):207-210.
[49]Duffy JB.GAL4 system in Drosophila:a fly geneticist's Swiss army knife.Genesis,2002,34(1-2):1-15.
[50]Li KX,Ma EB,Zhang JZ,Zhao YD,Zhang XB.Combination of Gal80tsand Gal4 flexibly manipulates the expression levels of UAS transgenes in drosophila.Acta Entomol Sin,2016,59(5):481-488.李开霞,马恩波,张建珍,赵一丹,张徐波.Gal80ts与Gal4组合灵敏控制果蝇中UAS转基因的表达水平.昆虫学报,2016,59(5):481-488.
[51]Deng YW,Li JH.Teaching reform of genetics experiment course based on improving college students'innovative ability.Course Educ Res,2018,(7):247-248.邓岳文,李俊辉.基于提高大学生创新能力的遗传学实验课教学改革.课程教育研究,2018,(7):247-248.
[52]Pi Y,Su MJ,Wei LQ,Lu DR,Qiao SY.An exploration of teaching in the genetic experiments--developing a type with classic experiments and new technology fused(1).Biol Teach Univ Elect Edit,2017,7(3):53-57.皮妍,苏明杰,韦理强,卢大儒,乔守怡.遗传学实验的教学改革--经典实验与新技术相融合(一).高校生物学教学研究(电子版),2017,7(3):53-57.
[53]Guo B,Mo AW,Cai L,Qiao SY,Pi Y.An exploration of teaching in the genetic experiments--developing a type with classic experiments and new technology fused(2).Biol Teach Univ Elect Edit,2018,8(1):56-60.郭滨,莫翱溦,蔡亮,乔守怡,皮妍.遗传学实验的教学改革--经典实验与新技术的融合(二).高校生物学教学研究(电子版),2018,8(1):56-60.
[54]Li BC.Animal genetics.Beijing:China Agricultural University Press,2008.李碧春.动物遗传学.北京:中国农业大学出版社,2008.