大孔吸附树脂纯化锥栗壳色素的工艺研究
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摘要
为获得高纯度的锥栗壳色素,比较7种大孔吸附树脂(NKA-9、D4020、XAD-7、DA-201、AB-8、X-5、D101)对锥栗壳粗提色素的静态吸附与解吸性能,筛选出最佳的大孔树脂,并通过单因素试验和正交试验考察其最佳的动态吸附工艺条件。结果表明,D101型大孔树脂对锥栗壳粗提色素有较好的吸附和解析效果,其动态吸附纯化的最佳工艺条件为:吸附柱径高比1:10,上样流速1.5 mL/min,上样浓度0.100 mg/mL。利用80%乙醇溶液作为洗脱剂,洗脱流速1.0 mL/min时解吸率最大。采用此纯化及解吸条件,锥栗壳色素的色价从31.2提高至74.3。纯化后的色素易溶解,溶液呈深红棕色、澄清透亮、稳定性好。
In order to obtain high purity pigment,seven types of macroporous resin(NKA-9,D4020,XAD-7,DA-201,AB-8,X-5,D101)were comparatively studied for their adsorption and desorption properties towards crudely extracted Castanea henryi shell pigment in this paper. The optimal macroporous resin was selected and the optimum dynamic adsorption technological conditions were investigated by single factor experiment and orthogonal experiment. The results showed that D101 macroporous resin had better adsorption and desorption effect on the crudely extracted Castanea henryi shell pigment,and the optimum process conditions for dynamic adsorption and purification of pigment were as follows:adsorption column diameter-height ratio 1:10,sample flow rate 1.5 mL/min,sample concentration 0.100 mg/mL. Using 80 % ethanol solution as eluant,desorption rate was the highest when the elution flow rate was 1.0 mL/min. The color value of Castanea henryi shell pigment was increased from 31.2 to 74.3 under the above purification and desorption conditions. Purified pigment was easily dissolved and its solution was crimson brown,clear and bright,and stable.
In order to obtain high purity pigment,seven types of macroporous resin(NKA-9,D4020,XAD-7,DA-201,AB-8,X-5,D101)were comparatively studied for their adsorption and desorption properties towards crudely extracted Castanea henryi shell pigment in this paper. The optimal macroporous resin was selected and the optimum dynamic adsorption technological conditions were investigated by single factor experiment and orthogonal experiment. The results showed that D101 macroporous resin had better adsorption and desorption effect on the crudely extracted Castanea henryi shell pigment,and the optimum process conditions for dynamic adsorption and purification of pigment were as follows:adsorption column diameter-height ratio 1:10,sample flow rate 1.5 mL/min,sample concentration 0.100 mg/mL. Using 80 % ethanol solution as eluant,desorption rate was the highest when the elution flow rate was 1.0 mL/min. The color value of Castanea henryi shell pigment was increased from 31.2 to 74.3 under the above purification and desorption conditions. Purified pigment was easily dissolved and its solution was crimson brown,clear and bright,and stable.
引文
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[16]姚丽敏,旷慧,张龙.大孔树脂纯化榛子壳棕色素粗提液的研究[J].食品工业科技,2014,35(20):317-321
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[20]王金亭,鞠秀萍,吴广庆.大孔吸附树脂纯化紫荆花红色素的研究[J].食品研究与开发,2009,30(4):49-53
[2]张发备.锥栗高产栽培技术[J].福建农业科技,2011(1):31-32
[3]蔡可柳.锥栗乌壳长芒品种的特征特性及栽培要点.[J].浙江农业科学,2009,1(2):278,281
[4]魏剑生.建瓯锥栗亩产十年翻四倍[EB/OL].(2015-10-31).http://news.ifeng.com/a/20151031/46062293_0.shtml.
[5]陈世平.建瓯市锥栗深加工产业快速发展[J].中国果业信息,2012,29(9):48-49
[6]刘辉,魏剑生.建瓯锥栗:名品如何变名牌?[N].福建日报,2017-10-31(06)
[7]黄艳,黄晓菲,吴琳珊,等.响应面法优化恒温-回流提取锥栗壳色素工艺[J].食品安全质量检测学报,2018,9(4):897-904
[8]黄俊娴,梁荣选,杨建男.天然色素的提取方法和应用[J].广东化工,2006,33(6):63-66
[9]徐青,卢莹莹,辛建美,等.大孔树脂吸附分离海芦笋中黄酮类化合物工艺[J].食品科学,2011,32(2):115-119
[10]孟维,李湘洲,吴志平,等.AB-8型大孔吸附树脂分离纯化茶皂素工艺[J].食品科技,2013(9):224-228
[11]王锋,谭兴和,郭时印,等.大孔树脂纯化黑花生衣色素的研究[J].湖南农业大学学报(自然科学版),2007,33(4):500-505
[12]展亚莉,刘晓波,展康华,等.大孔树脂吸附法纯化精制板栗壳天然棕色素的研究[J].中国食品添加剂,2015(5):105-109
[13]张海晖,段玉清,李金凤,等.板栗壳中原花青素大孔吸附树脂分离纯化工艺优化[J].农业机械学报,2011,42(5):156-159,155
[14]刘平.板栗壳棕色素提取、纯化及抗氧化性的研究[D].武汉:武汉工业学院,2008:28-40
[15]王金玲,姚丽敏,旷慧.榛子壳棕色素的分离纯化及其理化性质的研究[J].食品与生物技术学报,2016,35(7):770-777
[16]姚丽敏,旷慧,张龙.大孔树脂纯化榛子壳棕色素粗提液的研究[J].食品工业科技,2014,35(20):317-321
[17]唐红,谭沙,吴栋.大孔树脂分离红花中羟基红花黄色素A的优化工艺[J].广州化工,2017,45(8):50
[18]周俊良,沈佳奇,马玉华,等.大孔树脂吸附法纯化火龙果皮甜菜色素工艺[J].贵州农业科学,2017,45(4):112-115
[19]赵昕,常晓娟,展亚莉,等.大孔树脂分离纯化黑胡萝卜红色素的研究[J].中国食品添加剂,2017(3):68-73
[20]王金亭,鞠秀萍,吴广庆.大孔吸附树脂纯化紫荆花红色素的研究[J].食品研究与开发,2009,30(4):49-53