苯甲酸、富马酸及其复合酸对常见致病菌体外抑菌效果的研究
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摘要
旨在探讨苯甲酸、富马酸及其两种酸组合的复合酸对常见致病菌的体外抑菌效果.选取致病性大肠杆菌(ATCC83912)、沙门菌(ATCC21512)、金黄色葡萄球菌(ATCC65213)三种常见的致病菌为指示菌,苯甲酸、富马酸和复合酸(苯甲酸与富马酸按1:1的比例复合)浓度设置0. 25、0. 50、0. 75、1. 00 g/m L四个水平,以无菌水为空白对照.采用牛津杯法测定不同有机酸制剂的抑菌圈直径(mm),以此评价三种不同有机酸制剂体外抑菌活性.结果表明,有机酸对三种致病性菌具有明显的体外抑菌活性.其中,复合酸对大肠杆菌和金黄色葡萄球菌的抑菌效果显著优于富马酸和苯甲酸(P <0. 05),而对于沙门菌,苯甲酸和复合酸的抑菌效果要显著优于富马酸(P <0. 05);随着有机酸浓度的升高,抑菌效果显著增强(P <0. 05);不同有机酸制剂和浓度二因素对沙门菌和金黄色葡萄球菌存在显著的互作影响(P <0. 05).综上所述:在0. 25～0. 75 g/m L浓度下,复合酸对大肠杆菌、沙门菌和金黄色葡萄球菌的抑菌活性更优于苯甲酸、富马酸单一使用时的效果.
This study was conducted to investigate the antibacterial effect of benzoic acid,fumaric acid and two kinds of compound acids against common pathogenic bacteria in vitro. Pathogenic Escherichia coli( ATCC83912),Salmonella( ATCC21512)and Staphylococcalaureus( ATCC65213) were selected as indicator bacteria in the experiment. The concentration of benzoic acid,fumaric acid and compound acids( benzoic acid was compounded with fumaric acid by 1: 1) was set to 0. 25,0. 50,0. 75 and 1. 00 g/m L,respectively,compared with sterile water as the blank control. The antibacterial effects of three different organic acids were evaluated by measuring the antibacterial circle diameter by the Oxford Cup method. The results showed that organic acids had significant antibacterial activity against three pathogenic bacteria in vitro. The antibacterial effect of the compound acid against Escherichia coli and Staphylococcalaureus was significantly higher than those of fumaric acid and benzoic acid( P <0. 05). The antibacterial effect of benzoic acid and compound acid against Salmonella were significantly better than that of fumaric acid( P < 0. 05). With the concentration of organic acid increasing,the antibacterial effect gradually increased( P <0. 05). There were significant interactions between different organic acid types and concentrations on Salmonella and Staphylococcus aureus( P < 0. 05). In conclusion,at a concentration of 0. 25 g/m L to 0. 75 g/m L,the antibacterial effect of the compound acids against Escherichia coli,Salmonella and Staphylococcus aureus is better than those of benzoic acid and fumaric acid alone.
This study was conducted to investigate the antibacterial effect of benzoic acid,fumaric acid and two kinds of compound acids against common pathogenic bacteria in vitro. Pathogenic Escherichia coli( ATCC83912),Salmonella( ATCC21512)and Staphylococcalaureus( ATCC65213) were selected as indicator bacteria in the experiment. The concentration of benzoic acid,fumaric acid and compound acids( benzoic acid was compounded with fumaric acid by 1: 1) was set to 0. 25,0. 50,0. 75 and 1. 00 g/m L,respectively,compared with sterile water as the blank control. The antibacterial effects of three different organic acids were evaluated by measuring the antibacterial circle diameter by the Oxford Cup method. The results showed that organic acids had significant antibacterial activity against three pathogenic bacteria in vitro. The antibacterial effect of the compound acid against Escherichia coli and Staphylococcalaureus was significantly higher than those of fumaric acid and benzoic acid( P <0. 05). The antibacterial effect of benzoic acid and compound acid against Salmonella were significantly better than that of fumaric acid( P < 0. 05). With the concentration of organic acid increasing,the antibacterial effect gradually increased( P <0. 05). There were significant interactions between different organic acid types and concentrations on Salmonella and Staphylococcus aureus( P < 0. 05). In conclusion,at a concentration of 0. 25 g/m L to 0. 75 g/m L,the antibacterial effect of the compound acids against Escherichia coli,Salmonella and Staphylococcus aureus is better than those of benzoic acid and fumaric acid alone.
引文
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[13]乔军,孟庆龄,贾桂珍.运用OD值法进行细菌计数的研究[J].中国家禽,1996(4):26-27.
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[15]马绪荣,苏德模.药品微生物学检验手册[M].北京:科学出版社,2001.
[16]刘冬梅,李理,杨晓泉,等.用牛津杯法测定益生菌的抑菌活力[J].食品研究与开发,2006,27(3):110-111.
[17]郭洋,何利昆,马晓威.五种中药对仔猪白痢大肠杆菌的体外抑制试验[J].现代畜牧兽医,2009,(4):5 4-55.
[18]段晨磊,李文立.延胡索酸的作用机理及其在畜禽生产中的应用[J].中国饲料,2013(5):10-12.
[19]殷海琴.天然抗菌物质群组的提取及其在化妆品中的应用研究[D].广州:华南理工大学,2014.
[20]张军,田子罡,王建华,等.有机酸抑菌分子机理研究进展[J].畜牧兽医学报,2011,42(3):323-328.
[21]REUT HAZAN,ALEXANDRA LEVINE,HAGAI ABELIOVICH.Benzoic acid,a weak organic acid food preservative,exerts specific effects on intracellular membrane trafficking pathways in saccharomyces cerevisiae[J].Applied and Environmental Microbiology,2004,70(8):4449-4457.
[22]ISLAM M N.Inhibition of mold in bread by dimethyl Fumarate[J].Journal of Food Science,2010,47(5):1710-1712.
[23]丁发源,臧莹安.再认识酸化剂发展绿色畜禽业[J].中国禽业导刊,2004(14):37-38.
[24]吴秋珏,王建军,吕佳琪,等.富马酸对动物胃肠道微生物的影响研究[J].饲料工业,2011,32(4):52-53.
[25]秦圣涛,张宏福,唐湘方,等.酸化剂主要生理功能和复合酸选配依据[J].动物营养学报,2007,19(s1):515-520.
[2]何忠武,INDRESH H C.有机酸和植物提取物作为抗生素替代品是可能的有效选择[J].国外畜牧学(猪与禽),2008,28(2):48-50.
[3]张丽娟.苯甲酸衍生物对酪氨酸酶的效应及其抑菌作用[D].厦门:厦门大学,2006.
[4]DIBNER J J,BUTTIN P.Use of organic acids as a model to study the impact of gut microflora on nutrition and metabolism[J].Journal of Applied Poultry Research,2002,11(4):453-463.
[5]HARDY B.The issue of antibiotic use in the livestock industry:what have we learned?[J].Animal Biotechnology,2002,13(1):129.
[6]MROZ Z.Organic acids as potential alternatives to antibiotic growth promoters for pigs[J].Advances in Pork Production,2005,16(2):169-182.
[7]KRISTENSEN N B,NRGAARD J V,WAMBERG S,et al.Absorption and metabolism of benzoic acid in growing pigs[J].Journal of Animal Science,2009,87(9):2815-2822.
[8]BUHLER K,BUCHER B,WENK C,et al.Influence of benzoic acid in high fibre diets on nutrient digestibility and VFA production in growing/finishing pigs[J].Archives of Animal Nutrition,2009,63(2):127-136.
[9]KLUGE H,BROZ J,EDER K.Effect of benzoic acid on growth performance,nutrient digestibility,nitrogen balance,gastrointestinal microflora and parameters of microbial metabolism in piglets[J].Journal of Animal Physiology&Animal Nutrition,2010,90(7-8):316-324.
[10]PAPATSIROS V G,TASSIS P D,TZIKA E D,et al.Effect of benzoic acid and combination of benzoic acid with a probiotic containing Bacillus cereus var.Toyoi in weaned pig nutrition[J].Polish Journal of Veterinary Sciences,2011,14(1):117-25.
[11]LAWLOR P G,LYNCH P B,CAFFREY P J.Effect of creep feeding,dietary fumaric acid and level of dairy product in the diet on post-weaning pig performance[J].Irish Journal of Agricultural&Food Research,2005,44(1):45-55.
[12]丁月云,余大华,孟云,等.中草药复方对猪常见致病菌的体外抑菌试验[J].江苏农业学,2013,41(11):236-238.
[13]乔军,孟庆龄,贾桂珍.运用OD值法进行细菌计数的研究[J].中国家禽,1996(4):26-27.
[14]钱存柔,黄仪秀.微生物学实验教程[M].北京:北京大学出版社,2000.
[15]马绪荣,苏德模.药品微生物学检验手册[M].北京:科学出版社,2001.
[16]刘冬梅,李理,杨晓泉,等.用牛津杯法测定益生菌的抑菌活力[J].食品研究与开发,2006,27(3):110-111.
[17]郭洋,何利昆,马晓威.五种中药对仔猪白痢大肠杆菌的体外抑制试验[J].现代畜牧兽医,2009,(4):5 4-55.
[18]段晨磊,李文立.延胡索酸的作用机理及其在畜禽生产中的应用[J].中国饲料,2013(5):10-12.
[19]殷海琴.天然抗菌物质群组的提取及其在化妆品中的应用研究[D].广州:华南理工大学,2014.
[20]张军,田子罡,王建华,等.有机酸抑菌分子机理研究进展[J].畜牧兽医学报,2011,42(3):323-328.
[21]REUT HAZAN,ALEXANDRA LEVINE,HAGAI ABELIOVICH.Benzoic acid,a weak organic acid food preservative,exerts specific effects on intracellular membrane trafficking pathways in saccharomyces cerevisiae[J].Applied and Environmental Microbiology,2004,70(8):4449-4457.
[22]ISLAM M N.Inhibition of mold in bread by dimethyl Fumarate[J].Journal of Food Science,2010,47(5):1710-1712.
[23]丁发源,臧莹安.再认识酸化剂发展绿色畜禽业[J].中国禽业导刊,2004(14):37-38.
[24]吴秋珏,王建军,吕佳琪,等.富马酸对动物胃肠道微生物的影响研究[J].饲料工业,2011,32(4):52-53.
[25]秦圣涛,张宏福,唐湘方,等.酸化剂主要生理功能和复合酸选配依据[J].动物营养学报,2007,19(s1):515-520.